Cd101 (NM_001271217) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Igsf2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217414 representing NM_001271217 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAGCATCCTGTGTGTGGCATCTCTCTTTCTCTGTCTAACTAAGCTCAGCATCAGCCAGAGGGAAG TGAAAATACAAGAAGGACCTCTGTACAGAACCGAAGGTTACCCTGTCAGCATCAGTTGCAACGTAAGTGG TCATCGGGGTCCTTCTACACAGGATTTCCAGTGGTCTATTTACCTGCCAAGTGCCCCGACCCAAGAAGTC CAGATCATCAGTTCCAAGGATGCCGGCTTCTCTTACACCATGTATGCCCGGAGGGTGCAGAGCAAGGAGA TCTATGTAGAGAGGCTGCGGGGCAACTCGGTCCTGCTGCGTTTCTCGAAACTCCAGATGAAGGATACTGG CGAGTATGAGTGCCACACACCCAACACGGACGGAAAGTACTTTGGAAGTTACAGTGCAAAGACATATCTA ACTGTGATTCCCGACACCCTGTCTGCCACCATGCCCTCCCAGACACTCGGTAAGAAGGAAGGCGAGCCGT TGGAACTCACCTGCGAGGTGACCAAAGCCACGACTCAACATACCCATCTCTCCCTCACCTGGTACCTGAC GCAGGAAGGAGGAGGGAGCCACGCCACCGAGATCATTTCTCTCTCCAAGGATTTTATATTGACCCCTGGG CCCTCCTACACAGACAGATTTGTGGCCGGCGATGTACGGCTGGACAAGCTTGGAGCCACTTTCTTCAAAC TGTCCATAGGCAAGCTCCAGCTCTCAGATCAAGGCCATGTGTTCTGCGAGGCCACAGAGTGGATTCAGGA TCCAGATGAAGCATGGACTTTGATCACAAGAAAGCAGACAGATCAAACAGCTCTGAAGATCCAGCCAACA GCAAGAGATTTTGAAGTGAGCATCACGGCCAGCAGCTCCCCTGATGAAGGGAAACCCTTGGAACTGGTCT GCCTGGCTGTCGGCAGAGGAGGTGACCCACAGCTTCAGGGTACTTGGTTCCTCGATGGGAAGGAAATTGC CCAGATTGATGCTCGCGGGATCCTGGATCTGAAGAGAGACTACAGAGACAGAGCAAGCCAAGGACAGCTG CAGGTTTCAAAGTTAAGTGCCCAGACTTTCTCTCTCAAGATCTTCTCCGTGGGTCCGGAGGACGTAGGCG CCTACACTTGTGAAGTGGCAGAGGTGGTGAGGACTCAGACAGCCTCCTGGCAGGTACTTCAGAGAAAGCA GTCAGTAGGCCACCCAGTGCAGCTGAGGGAGCCGGCAGCAAGAAGTGTGACCGTGTCTGCAGAGCAGCGT GCCGTGTGGGAAGGAGAGACAGTCACCCTTCTCTGCAAGGCAGCTGGAGATGCAGCTGCTCTGTCTGTCA GTTGGTGGCTCACCCCACAGGACCAGACCACACCCGTGTTTGTGGCTGGCATGGGGCAAGATGGTATTGT GCAGCCAGGAGTCTCTTCTCCCGGCCCTGGGTACCATGGTAACAGGAGGTTGGAGAAAGTGGACTGGGCC ACCTTTCGGCTGGAGATTGCTTCTGCCACGGTCACAGACAGCGGTACTTATGAATGCAGAGTGTCAGAGA GACCCCAGAACCAGGCCAAAGATTTGCAGTCAACCCAGAAGATTTCAGTCACAGTAAAATCTCTGAAATC AAGTTTGCGAGTTAATCTGATGAGCCGGCAGCCACAAGTGATGTTAGCCCATACCTTCGACCTGTCTTGT ATAGTGAGGGCCAACTACTCGGATCTCAAGCTGCCATTCTCCGTGATGTGGCAGTTCCAGCCAGCTGGCT CTGGAGCCTTCCATCAGCTTATTCGAGTCACCCATGATGGCCGAGTTGAATGGGGGGACACCCTCCCCCA GCTCCACAGGAAGACGAAGGTGTCACAGTCTTTCTTTCGTTCTCAACTCCAAATCCACGATGCTGCCATG GAGGAGACAGGGGTGTATCGGTGCGAAGTGGAGGTTTATGACAGAAATTCCGTGTGCACAAGTGGCCCGG CCAGGGTGTCTGCCACCTCTAACCTATTAATGATTACAGTCACCTTTCCAGAGAGCAAACTGAGAGTGAA CTCGAGCAGTCAGGTCCAAGAGCTAACCATCAGCTCCAGCACTCAAATAGAATGTGCCATCTTGTCCCGA TCTGCTGGAAACCTTCCGTTATCCATCATTTGGTACTTCTCTCCCATTTCTACAAATGCATCCCACTTGA AAATCCTAGAAATGGACCAAACCAATGTTGTAAAATATGGGGATGAATTTCAAACCCCTCGGAGCAAGCA AAAATTTTATTCCGAGAAAGTTTCCCAAGACTTATTTCTGCTGAACATTCTGAGCATGGAGGACAGTGAC CAGGGCTGCTACCACTGTGCTGTAGAGGAATGGCTCTTGTCTACAAATGGCACTTGGCAAAAGCTTGAAA GGAAGACTTCAGGACTCACAGAATTGAAACTCAGGCCCACAGGAAGTCAGGTCCATGTCTCCAAAGTAAG CTGGAAGGGAAATGCTACAGAGTATGGAGAGGCGGGCTTCAGCTGCAGCCTGGACGGCCCTGGTAGCACA TCCTCCCTGTACTCTGTGACTTGGTACTGGAGCAAAGGAACCGCTGTGGACGGGAGTCAAATGCTGGTGC ACCTGCAGTATGACGGCTTGCTGCAGTATGGCCAAGAGGGGAGCAGGAAGCTCCTGGACTGCTACCGATC CTCTTCCACAGATTTTGTCCTGAAGCTGCATCGGGTGGAAATGGAGGATGCTGGGATATATTGGTGCAGG GTGGCTGAGTGGCAGCAATATGGCCACCCAGGCAAGTGGATCAACCAAGCCTCAGATGAATCACAACGAA TGGTACTCCGGGTGCTGCGCTCAGAGTCCACGTTCTCTTCCCGGATCTGCTCCTCTGGACCCTTACTCCA CTTCCTCGTCGTCTGCCCCTTCCTCATGTTGCTCCTTCTATTCACGTCCTGCCTCTGCTTGTACTGGAAG GCCAGGAAGTTGTCAGACCTGAGTCTCAGTTCGAAGAAAGAAAAGGCTCTTTGGGTGGGCATGAGGAGGA CATCACTTCAGAAGGAAGGAGGAGAGGAGGGTGAAGGCTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217414 representing NM_001271217 Red=Cloning site Green=Tags(s) MASILCVASLFLCLTKLSISQREVKIQEGPLYRTEGYPVSISCNVSGHRGPSTQDFQWSIYLPSAPTQEV QIISSKDAGFSYTMYARRVQSKEIYVERLRGNSVLLRFSKLQMKDTGEYECHTPNTDGKYFGSYSAKTYL TVIPDTLSATMPSQTLGKKEGEPLELTCEVTKATTQHTHLSLTWYLTQEGGGSHATEIISLSKDFILTPG PSYTDRFVAGDVRLDKLGATFFKLSIGKLQLSDQGHVFCEATEWIQDPDEAWTLITRKQTDQTALKIQPT ARDFEVSITASSSPDEGKPLELVCLAVGRGGDPQLQGTWFLDGKEIAQIDARGILDLKRDYRDRASQGQL QVSKLSAQTFSLKIFSVGPEDVGAYTCEVAEVVRTQTASWQVLQRKQSVGHPVQLREPAARSVTVSAEQR AVWEGETVTLLCKAAGDAAALSVSWWLTPQDQTTPVFVAGMGQDGIVQPGVSSPGPGYHGNRRLEKVDWA TFRLEIASATVTDSGTYECRVSERPQNQAKDLQSTQKISVTVKSLKSSLRVNLMSRQPQVMLAHTFDLSC IVRANYSDLKLPFSVMWQFQPAGSGAFHQLIRVTHDGRVEWGDTLPQLHRKTKVSQSFFRSQLQIHDAAM EETGVYRCEVEVYDRNSVCTSGPARVSATSNLLMITVTFPESKLRVNSSSQVQELTISSSTQIECAILSR SAGNLPLSIIWYFSPISTNASHLKILEMDQTNVVKYGDEFQTPRSKQKFYSEKVSQDLFLLNILSMEDSD QGCYHCAVEEWLLSTNGTWQKLERKTSGLTELKLRPTGSQVHVSKVSWKGNATEYGEAGFSCSLDGPGST SSLYSVTWYWSKGTAVDGSQMLVHLQYDGLLQYGQEGSRKLLDCYRSSSTDFVLKLHRVEMEDAGIYWCR VAEWQQYGHPGKWINQASDESQRMVLRVLRSESTFSSRICSSGPLLHFLVVCPFLMLLLLFTSCLCLYWK ARKLSDLSLSSKKEKALWVGMRRTSLQKEGGEEGEGY TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271217
ORF Size	3051 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271217.1, NP_001258146.1
RefSeq Size	3122 bp
RefSeq ORF	3054 bp
Locus ID	310727
MW	113.3 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.