Tll2 (NM_001191898) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1559756; Tll2b
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217411 representing NM_001191898 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCAGGCGACCGCTCTTGGCACCCTGGTGCTACTGTTGCTGCTACCGCTACTCCTCCGTGCTGAAG TGACTGGGGACCACTCGGATGTCGCGTTGGATTACGGGGCCTTGGAAGGCGAGGAGGGCACGGAGCAGGA GCAGCAGCTGCAATACCACGACCCCTGCAAAGCTGCTGTCTTCTGGGGAGATATCGCCTTGGATGAAGAT GATCTCAAGTTATTTCACATTGACAAGGCTGAAGACTGGACCAAGCCATCCATGGAAAGAATGGGACACG ACACTGGTGGCCTTGAGGAAACATCTGCAAGGTGGCCAAATGATACAGCCTCTAACAGCAGCATCCAGGC ACTAAGAAAGGATGGCAAGGATAGCACCACACTTCTGCCTGGCCCTGGGACCTCAAACACCACCGCTAAG ACCTTCTCTGCTCGGGTCCGAAGAGCTACAACCTCAAGGACAGAGCGGATATGGCCTGGAGGGGTCATTC CTTATGTCATCGGAGGGAACTTTACTGGCACCCAGAGGGCCATTTTCAAACAGGCCATGAGGCACTGGGA GAAGCACACCTGTGTGACCTTCATAGAGAGGACAGACGAGGAGAGCTTCATTGTTTTCAGTTACAGGACC TGTGGCTGTTGTTCCTACGTGGGACGCCGAGGAGGTGGCCCTCAGGCCATATCCATTGGGAAAAACTGTG ACAAGTTCGGCATCGTGGCTCACGAGCTGGGCCACGTGGTTGGGTTCTGGCACGAACACACTCGGCCAGA CCGAGACCAGCATGTCACCATCATCAGAGAAAACATCCAGCCAGGTCAGGAGTATAATTTCTTAAAAATG GAAGCCGGCGAGGTGAGCTCTCTGGGAGAGACCTACGACTTCGACAGCATCATGCACTATGCCCGGAACA CCTTCTCAAGAGGAGTTTTCTTAGACACCATCCTCCCCCGTCGAGACGACAATGGCGTCAGGCCAACCAT TGGCCAACGCGTGCGGCTCAGTCAGGGAGATATAGCTCAAGCCAGGAAGCTGTACAAATGCCCAGCGTGT GGGGAAACACTACAGGACACATCAGGAAACTTCTCTGCACCTGGTTTCCCAAATGGCTACCCCTCTTATT CCCACTGCGTCTGGAGGATCTCCGTCACCCCAGGGGAAAAGATCATCCTAAATTTCACCTCCATGGATTT GTTTAAGAGCCGCTTGTGCTGGTACGATTACGTGGAGATCCGGGATGGTTACTGGAGAAAGGCCCCCCTG TTGGGGAGGTTTTGCGGCGATAAGGTTCCTGAGCCCCTCGTCTCCTCGGACAGCCGGCTGTGGGTGGAAT TCCGTAGTAGCAGCAGCATCCTGGGCAAGGGCTTCTTCGCTGTCTATGAAGCCATGTGTGGGGGAGACAT AACTAAAGATGCGGGCCAGATTCAATCTCCCAACTACCCTGACGACTACAGGCCTTCCAAGGAATGTGTG TGGAGAATCACAGTAGCAGACGGGTTTCATGTGGGGCTTACCTTCCAGTCCTTTGAGATTGAAAGACATG ACAGTTGTGCATATGACTATCTGGAAATCCGAGACGGTCCCACAGAGGAGAGCGCCCTGATTGGCCATTT CTGTGGCTACGAGAAGCCAGAGGCTGTGAAATCCAGTGCCAACCGACTATGGGTGAAGTTTGTGTCGGAC GGCTCCATCAATAAAGCAGGCTTTGCAGCCAGTTTCTTCAAGGAGGTGGATGAGTGCTCCTGGCCAGACC ATGGTGGGTGTGAGCAGCGCTGTGTAAACACACTCGGCAGCTACACGTGTGCCTGTGACCCTGGCTACGA GCTAGCTGCTAACAAGAAGACATGTGAAGTGGCCTGTGGTGGATTTATTACCAAGCTAAACGGCACCATC ACCAGCCCTGGATGGCCGAAGGAGTACCCCACAAACAAGAACTGTGTCTGGCAGGTGGTGGCCCCCATGC AGTACCGCATCTCCCTGCAGTTCGAAGCATTCGAGTTGGAAGGCAATGACGTTTGTAAGTATGACTTCGT AGAGGTGCGCAGTGGCCTGTCCCCAGATGCCAAGCTTCATGGCAAGTTCTGTGGCTCCGAGACCCCGGAG GTCATCACATCTCAGAGCAACAACATGCGCGTGGAGTTCAAGTCCGACAACACCGTCTCCAAGCGAGGCT TCAGGGCTCACTTCTTCTCAGACAAAGACGAGTGTGCCAAAGACAATGGCGGCTGCCAGCAGGAGTGTGT CAACACATTTGGGAGCTACCTGTGCCGATGCAGGAATGGGTACCGGCTGCACGAGAACGGACATGACTGC AAAGAGGCTGGCTGTGCATACAAGATCAGCAGTGCAGAGGGAACCCTGATGAGCCCCAACTGGCCTGACA AATACCCCAGCCGGAAGGAATGTACCTGGAACATTTCATCAACTGCAGGCCACAGGGTGAAAATTACATT CAGTGAGTTTGAGGTTGAGCAGCATCAGGAATGCGCCTACGACCACCTGGAACTGTATGACGGGACGGAC AGCTCGGCCCCCATCCTCGGCCGCTTCTGTGGCAGCAAGAAGCCGGATCCCGTGGTGGCTACAGGCAGCA GCCTGTTCCTCAGGTTTTACTCAGATGCCTCGGTGCAGAGGAAAGGTTTCCAGGCTGTGCACAGCACAGA ATGTGGAGGCAGACTGAAGGCTGAAGTGCAGACCAAAGAGCTCTACTCTCATGCCCAGTTTGGGGACAAC AACTACCCGAGCCAGGCCCACTGTGACTGGGTGATAGTCGCGGAAGACGGTTATGGCGTGGAGCTGGTAT TCCGGACCTTTGAAGTGGAGGAGGAAGCTGACTGTGGCTATGACTACTTGGAGGCCTATGATGGGTACGA CAGCTCAGCACCCAGGCTTGGCCGCTTCTGTGGCTCAGGGCCGTTAGAGGAAATCTACTCTGCAGGAGAC TCACTGATGATTCACTTCCACACGGACGACACCATCAACAAGAAAGGCTTTCACGCCCGATACACCAGCA CCAAGTTCCAAGATGCCTTGCACATGAGGAAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217411 representing NM_001191898 Red=Cloning site Green=Tags(s) MPQATALGTLVLLLLLPLLLRAEVTGDHSDVALDYGALEGEEGTEQEQQLQYHDPCKAAVFWGDIALDED DLKLFHIDKAEDWTKPSMERMGHDTGGLEETSARWPNDTASNSSIQALRKDGKDSTTLLPGPGTSNTTAK TFSARVRRATTSRTERIWPGGVIPYVIGGNFTGTQRAIFKQAMRHWEKHTCVTFIERTDEESFIVFSYRT CGCCSYVGRRGGGPQAISIGKNCDKFGIVAHELGHVVGFWHEHTRPDRDQHVTIIRENIQPGQEYNFLKM EAGEVSSLGETYDFDSIMHYARNTFSRGVFLDTILPRRDDNGVRPTIGQRVRLSQGDIAQARKLYKCPAC GETLQDTSGNFSAPGFPNGYPSYSHCVWRISVTPGEKIILNFTSMDLFKSRLCWYDYVEIRDGYWRKAPL LGRFCGDKVPEPLVSSDSRLWVEFRSSSSILGKGFFAVYEAMCGGDITKDAGQIQSPNYPDDYRPSKECV WRITVADGFHVGLTFQSFEIERHDSCAYDYLEIRDGPTEESALIGHFCGYEKPEAVKSSANRLWVKFVSD GSINKAGFAASFFKEVDECSWPDHGGCEQRCVNTLGSYTCACDPGYELAANKKTCEVACGGFITKLNGTI TSPGWPKEYPTNKNCVWQVVAPMQYRISLQFEAFELEGNDVCKYDFVEVRSGLSPDAKLHGKFCGSETPE VITSQSNNMRVEFKSDNTVSKRGFRAHFFSDKDECAKDNGGCQQECVNTFGSYLCRCRNGYRLHENGHDC KEAGCAYKISSAEGTLMSPNWPDKYPSRKECTWNISSTAGHRVKITFSEFEVEQHQECAYDHLELYDGTD SSAPILGRFCGSKKPDPVVATGSSLFLRFYSDASVQRKGFQAVHSTECGGRLKAEVQTKELYSHAQFGDN NYPSQAHCDWVIVAEDGYGVELVFRTFEVEEEADCGYDYLEAYDGYDSSAPRLGRFCGSGPLEEIYSAGD SLMIHFHTDDTINKKGFHARYTSTKFQDALHMRK SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191898
ORF Size	3042 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191898.1, NP_001178827.1
RefSeq Size	3045 bp
RefSeq ORF	3045 bp
Locus ID	365460
MW	113.5 kDa

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