Adgrg2 (NM_001270871) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Gpr64; He6; Re6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217401 representing NM_001270871 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTTTCTCTGGGGGTCAGTACAGCCCTGTTGGCAGGCCTGAAGAGGTTTTACTGATATACAAGATAT TCCTTGTCATCATTTGTTTTCATGCCATTCTTGTTACATCCCTGAAAGAAAATGCTGGTAATTCCAGTTT GTTGTCACCATCTGATGCTGCTTCCGAAGTATTGTCGACTTTAAACAGAACAGAAAAATCTAAAATCACT ATATTAAAAACCTTCAATGCATCAGGAGTCAAATCCCAGAGAAATATCTGCAATTTGTCATCTATTTGCA GTGACTCAGTGTTTTTTAGAGGTGAGATAGTGTTTCAACATGATGACCACTACAACGTTACTCAGAATCA AGATATAGTTAACAGCACCTTCGCTGGAGTCCTGTCTCTAAGCGAACTGAAGCGAACAGAACTCAACAAA ACTCTACAGACCTTAAGTGAGACTTACTTTATAGTGTGTGCTACCGCAGAGGCCCAAAACACATTAAACT GTACATTCACAGTAAAACTGAATGAGACCATGAATGTGTGTGCCATGATGGTTACTTTCAAAAGTGTACA GATTCGGCCAATGGAACAGTGCTGCTGTTCCCCCAGGACTCCCTGCCCTTCCTCGCCGGAAGAGTTAGAA AAACTACAGTGTGATCTGCAGGATCCCATTGTCTGCCTTGCCGATCAACCACATGGCCCACCAGTATCTT CTTCCAGCAAGCCTGTGCCAGTTGTACCTCAGGCCACCATTTTTTCCCACGTTGCTAGTGACTTCTCTTT GGCTGAACCCCTTGATCATGCCCTTATGACCTCAAGCACACCCTCTCTGGCACAAGAGACACGCCTTCCA TCTCCTCAGCCTACAATCTCCCTGACTTCCAGTCCTGCCATTGACTTGCCTGTTCAACATGTAGTGGCTT CTTCCTCCTTGCCCCAAACTGATCTTTCCCACACCCTATCACCTGTGCAGTCCTCCATTCCCTCTCCTAC CACAGCAGCTCCATCTGTCCCTGAAAAAGTGGTCGCCATCAGCACACCTCCTGGCGAGACAGTTGTCAAC ACGAGTAGTGTTCCTGATCTGGAAGCCCAAGTATCTCAAATGGAGAAAGCTTTGTCTTTGGGCAGCTTGG AACCTAATCTTGCGGGTGAAATGGTGAACCGAGTCAGCAAACTCCTTCACTCTCCACTTGCCTTGCTAGC CCCTCTAGCTCAAAGGTTGCTAAAAGTGGTAGATGCCATTGGCTTACAGCTGAATTTTTCATCTACAACT ATCAGTCTAACTTCTCCTTCTTTGGCTCTTGCTGTGATCAGAGTGAATGCCAGTAATTTCAATACAACAA CCTTTGCAGCCCAAGACCCAGCAAATCTCCAGGTGTCTCTGGAAGCCCAGGCTCCTAAGAATAGTATTGG CGCCATTACTCTACCCTCATCGCTGATGAGTAATTTGCCAGCTAGTGAGGTAGAATTGGCTTCGAGGGTT CAGTTCAATTTCTTTGAAACACCTGCCCTATTTCAGGACCCCTCCCTGGAGAACCTCTCTCTGATAAGCT ATGTCATATCATCAAGTGTCACAAACATGACCATCAAGAACTTGACAAGAAACGTGACAGTTGCACTGAA GCACATCAACCCAAGTCAGGATGACTTAACTGTCAAATGTGTTTTCTGGGACTTGAACAGAAATGGTGGC AGAGGAGGTTGGTCATCTGATGGCTGTTCCGTCAAAGAGAAGAGGATGAACGAAACTATCTGTACCTGTA GCCACCTTACAAGTTTTGGCATCCTATTGGATCTATCTCGGACATCCTTACCACCAAGTCAAATGATGGC TCTGACATTTATCACGTATATTGGCTGTGGGCTTTCATCAATTTTTCTGTCAGTTACTCTTGTAACCTAT ATAGCCTTTGAAAAGATCCGGAGGGATTACCCTTCCAAAATCCTCATTCAACTGTGTGCTGCCCTGCTTC TGCTCAACCTGGTCTTCCTCTTAGACTCCTGGATTGCACTGTATAATGCCCGAGGTTTCTGCATCTCCGT GGCTGTATTTCTTCACTATTTTCTCTTGGTATCATTCACATGGATGGGCTTAGAAGCATTTCATATGTAC CTAGCACTGGTTAAGGTTTTTAATACTTACATCCGAAAGTACATCCTTAAATTCTGCATTGTTGGCTGGG GGATACCAGCTGTGGTTGTATCCATCGTCCTGACTATATCCCCAGATAATTATGGGATTGGATCTTATGG GAAATTCCCCAATGGCACACCAGATGACTTTTGCTGGATCAACAGCAGTGTGGTGTTCTATATCACGGTT GTGGGATATTTCTGTGTGATATTTCTACTGAACGTCAGCATGTTCATTGTGGTCTTGGTTCAGCTCTGTC GAATTAAAAAGAAGAAGCAACTGGGAGCGCAGCGCAAAACTAGTATTCAAGACCTCCGGAGTATTGCTGG CCTCACATTTTTACTGGGAATTACTTGGGGCTTTGCCTTCTTTGCCTGGGGACCAGTTAATCTCACCTTC ATGTATCTCTTTGCCATCTTTAACACCTTACAAGGCTTTTTCATATTCATCTTTTACTGTGCAGCAAAAG AGAATGTCAGAAAACAGTGGAGGCGGTATCTTTGTTGTGGAAAATTACGACTGGCTGAAAACTCTGACTG GAGTAAAACTGCTACTAATGGTTTAAAGAAGCAGACTGTAAATCAAGGAGTATCCAGCTCTTCAAATTCC TTACAGTCAAGCTGTAACTCCACTAACTCCACTACACTCCTAGTGAATAGTGATTGCTCAGTGCACGCAA GCGGGAATGGCAATGCATCTACCGAGAGGAATGGGGTTTCTTTCAGTGTTCAGAATGGAGACGTGTGCCT TCATGATCTCACTGGGAAACAGCACATGTTTAGTGACAAAGAGGACTCCTGCAATGGCAAAAGCCGGATG GCACTCAGAAGGACTTCAAAGCGGGGAAGCTTACACTTTATCGAGCAAATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217401 representing NM_001270871 Red=Cloning site Green=Tags(s) MLFSGGQYSPVGRPEEVLLIYKIFLVIICFHAILVTSLKENAGNSSLLSPSDAASEVLSTLNRTEKSKIT ILKTFNASGVKSQRNICNLSSICSDSVFFRGEIVFQHDDHYNVTQNQDIVNSTFAGVLSLSELKRTELNK TLQTLSETYFIVCATAEAQNTLNCTFTVKLNETMNVCAMMVTFKSVQIRPMEQCCCSPRTPCPSSPEELE KLQCDLQDPIVCLADQPHGPPVSSSSKPVPVVPQATIFSHVASDFSLAEPLDHALMTSSTPSLAQETRLP SPQPTISLTSSPAIDLPVQHVVASSSLPQTDLSHTLSPVQSSIPSPTTAAPSVPEKVVAISTPPGETVVN TSSVPDLEAQVSQMEKALSLGSLEPNLAGEMVNRVSKLLHSPLALLAPLAQRLLKVVDAIGLQLNFSSTT ISLTSPSLALAVIRVNASNFNTTTFAAQDPANLQVSLEAQAPKNSIGAITLPSSLMSNLPASEVELASRV QFNFFETPALFQDPSLENLSLISYVISSSVTNMTIKNLTRNVTVALKHINPSQDDLTVKCVFWDLNRNGG RGGWSSDGCSVKEKRMNETICTCSHLTSFGILLDLSRTSLPPSQMMALTFITYIGCGLSSIFLSVTLVTY IAFEKIRRDYPSKILIQLCAALLLLNLVFLLDSWIALYNARGFCISVAVFLHYFLLVSFTWMGLEAFHMY LALVKVFNTYIRKYILKFCIVGWGIPAVVVSIVLTISPDNYGIGSYGKFPNGTPDDFCWINSSVVFYITV VGYFCVIFLLNVSMFIVVLVQLCRIKKKKQLGAQRKTSIQDLRSIAGLTFLLGITWGFAFFAWGPVNLTF MYLFAIFNTLQGFFIFIFYCAAKENVRKQWRRYLCCGKLRLAENSDWSKTATNGLKKQTVNQGVSSSSNS LQSSCNSTNSTTLLVNSDCSVHASGNGNASTERNGVSFSVQNGDVCLHDLTGKQHMFSDKEDSCNGKSRM ALRRTSKRGSLHFIEQM TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001270871
ORF Size	2991 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001270871.1, NP_001257800.1
RefSeq Size	4560 bp
RefSeq ORF	2994 bp
Locus ID	266735
UniProt ID	Q8CJ11
MW	109.1 kDa
Gene Summary	epididymus specific member of the LNB-TM7 subfamily of seven transmembrane receptors [RGD, Feb 2006]

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