Myo19 (NM_001163736) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1559961
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217370 representing NM_001163736 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACCAGCAGGTGAATGGCCACAGTTTAGGCTCTGATACCCAAGGCAGGACATCCCTCAAGGGCGACC TAAGGGAGTTCCTGGGTGGGGAGGTCCCACTGCACCAGCTGGATGACCTCACCAAGGTGAATCCCGTGAC ACTGGAAACAGTCCTGAGGTGCCTGCAGGCCCGGTACACAGAAGACATATTCTATACCAATGCTGGCTGT ACCTTGGTAGCTCTGAACCCCTTTAAGCACATCCCTCAACTCTATGCACCAGAACTGATGCAAGAGTACC ATGCTGCACCTCAGCCTCAGAAATTAAAACCCCACATCTTCACAGTGGGTGAGCAGACCTACAGGAATGT CAAGAGCTTGATTGAGCCGATCAACCAATCCATTGTGGTCAGTGGAGAGAGCGGAGCAGGAAAGACATGG ACATCCCGATGCCTGATGAAGTTCTATGCTGTGGTGGCTGCCTCACCTACTTCCTGTGAGAACCACAAGA TGGCAGAGCGGATTGAAGAGCGGATCCTGAACTCCAACCCTGTCATGGAAGCTTTTGGGAACGCATGCAC ACTGAGGAATAACAACAGCAGTCGCTTTGGGAAGTTCATCCAGCTCCAGCTGAACAGAGCCCAACAGATG ACAGGAGCTGCAGTCCAGACCTACCTCCTAGAGAAAACCCGTGTGGCCTGTCAGGCCGCCAGTGAGAGGA ACTTCCACATCTTCTACCAGATTTGCAAAGGAGCCACCAAGGAAGAAAGGCTCCAGTGGTGCCTCCCTGA GGGAACTGCCTTCTCCTGGCTACCCAATCCAGAAAGCAGTCTGGAAGAGGATTGTTTCGAGGTGACCAGA GAGGCCATGCTCCATCTGGGCATCGACACCCCCACCCAGAACAACATCTTTAAGGTCCTAGCTGGACTAC TGCACCTTGGCAATGTCCACTTCGTTGACTCAGAGGATGAAGCCCAGCCCTGCCAACTGATGGATGGTAC CAAGGGCTCTGTCAGGACCTCAGCCTTGCTGCTGCAGCTCCCAGACAACATGCTGCTGGAGAGCATGCAG ATTCGAACCATCAAGGCAGGCAAGCAGCAGCAGGTGTTCCAGAAGCCCTGCTCCAGAGCTGAGTGTGACA CACGCAGAGACTGTCTGGCCAAACTGATCTATGCACGGCTATTCGACTGGCTGGTATCTGTGATCAACAG CAGCATCTGTGCAGACTTCAGCTCATGGACTGCCTTCATAGGTCTGCTAGATGTGTACGGGTTTGAGTCA TTTCCTGACAACAGTCTGGAACAGTTGTGCATCAATTATGCCAATGAGAAGCTACAGCAGCACTTCGTGG CTCACTACCTCAGGGCCCAGCAGGAGGAATATGAAGTTGAGGGCCTGGAGTGGTCATTTGTCAACTACCA GGACAACCAGACCTGTTTAGATCTCCTCGAAGGGAGCCCCATCAGCATCTGTTCCCTCATAAATGAGGAA TGCCGCCTTAACCGTCCGAGCAGTGCAGCACAACTACAGACACGCATTGAGAGCACGCTGGCAGGCAGGC CCTGCCTGGGCCATAACAAGCTCAGCCGGGAGCCCAGCTTTGTGGTGATGCATTTCGCAGGACCTGTACG GTACTGCACAGCGGGCCTAGTGGAAAAGAACAAGGACCCTGTTCCCCCTGAGCTGACTGGGCTCCTGCAG CAATCCCAAGACCCCCTGCTCATGATGCTGTTTCCTGCTAACCCTGAAGAGAAGACCCAGGAGGAGCTGT CTGGCCAGAGCAGGGCTCCTGCATTGACTGTGGTGTCCAAGTTCAAGGCCTCACTGGAACAGCTCCTGCA GGTCCTGCATAAAACAACCCCCCACTACATTCGCTGCATCAAGCCCAACAGCCAGAGTCAGCCGCAGACT TTCCTCCAGGAAGAGGTCCTGAACCAGTTAGAGGCCTGTGGTCTTGTGGAGACCATCCACATCAGTGCTG CTGGCTTTCCCATCCGGGTCTCTCACCAGAACTTCATGGAAAGATATAAATTGCTAACAAGACTCCGACC TCGTACATCCTCTGGCCTCAGGAGCCTGTGTCCTGCCAAAGGGTCCTCTGAGCAGCCTCTGTGTGCCAAG GAGGCCACACTGCAGCCTCTACTACAGGACATTCTCCATGCTCTGCCAGCTTTAATTCAGACAGCAACCA CTCCCAGTGACCCAGCTAAGACTGCACAAATCCCACTATACTGTGGCAGGACAAAGATTTTCATGACTGA CTCCATGCTAGAGCTTCTGGAATGTGGGCGTGCCCAGATGCTAGAGCAGTGTGCCCGCTGCATCCAGTGT GGCTGGAGGCGACACCGGCTCCAAAAGCAGGAGAAGCAGAGGCGGGCGGCCGTGATCATTCAGGCAGCTT TTCGGTCCTGGTTAACTCGGAAACACATCAGAAGGTTACACATAGCTGCCACAGTCATCAAGCACGCATG GCATAAGTGGAGAATCAGAATGGCCTGTCTTGCCTCTAAAGAACTGGATGGTATGGAGGAAAAACTCATG CCTCAGGCTCCCGGTACCCTGCGTTCCTCGCTGTCCCCAGCACACACTAGGTTCCTGGGAGCAATAATCC ACCTCTGGCCCCTGGGACTGGTGCTAGCCAACTCAGCTGATGGCATGCGTGGCTTTCAGAGGAAACTGGT AGCCCATGCCTGCCTTCGGCTTCCCGCAAGCGGCCCCAGCAACAAAGTCCAGACACCACAACAAGATCAG GCTGGTATCACATCCATCAGAGCACTGCCCCAGGGCTCAATAAAGTTCCACTGCAGGAAGTCTCCACTTC AGTATGCTGACATCTGCCCTGACCCTTCAGCCTCTAATGTTACTGGCTTTAATCAGATTCTGCTGCTAGA AAGACACAGGCCAGTCCAAGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217370 representing NM_001163736 Red=Cloning site Green=Tags(s) MHQQVNGHSLGSDTQGRTSLKGDLREFLGGEVPLHQLDDLTKVNPVTLETVLRCLQARYTEDIFYTNAGC TLVALNPFKHIPQLYAPELMQEYHAAPQPQKLKPHIFTVGEQTYRNVKSLIEPINQSIVVSGESGAGKTW TSRCLMKFYAVVAASPTSCENHKMAERIEERILNSNPVMEAFGNACTLRNNNSSRFGKFIQLQLNRAQQM TGAAVQTYLLEKTRVACQAASERNFHIFYQICKGATKEERLQWCLPEGTAFSWLPNPESSLEEDCFEVTR EAMLHLGIDTPTQNNIFKVLAGLLHLGNVHFVDSEDEAQPCQLMDGTKGSVRTSALLLQLPDNMLLESMQ IRTIKAGKQQQVFQKPCSRAECDTRRDCLAKLIYARLFDWLVSVINSSICADFSSWTAFIGLLDVYGFES FPDNSLEQLCINYANEKLQQHFVAHYLRAQQEEYEVEGLEWSFVNYQDNQTCLDLLEGSPISICSLINEE CRLNRPSSAAQLQTRIESTLAGRPCLGHNKLSREPSFVVMHFAGPVRYCTAGLVEKNKDPVPPELTGLLQ QSQDPLLMMLFPANPEEKTQEELSGQSRAPALTVVSKFKASLEQLLQVLHKTTPHYIRCIKPNSQSQPQT FLQEEVLNQLEACGLVETIHISAAGFPIRVSHQNFMERYKLLTRLRPRTSSGLRSLCPAKGSSEQPLCAK EATLQPLLQDILHALPALIQTATTPSDPAKTAQIPLYCGRTKIFMTDSMLELLECGRAQMLEQCARCIQC GWRRHRLQKQEKQRRAAVIIQAAFRSWLTRKHIRRLHIAATVIKHAWHKWRIRMACLASKELDGMEEKLM PQAPGTLRSSLSPAHTRFLGAIIHLWPLGLVLANSADGMRGFQRKLVAHACLRLPASGPSNKVQTPQQDQ AGITSIRALPQGSIKFHCRKSPLQYADICPDPSASNVTGFNQILLLERHRPVQV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001163736
ORF Size	2892 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001163736.1, NP_001157208.1
RefSeq Size	3841 bp
RefSeq ORF	2895 bp
Locus ID	497974
MW	108.3 kDa

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