Mib2 (NM_001005901) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217359 representing NM_001005901 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCTGGACCCCCATGCAGGTGTGCAGGTGGGCATGCGTGTGGTACGCGGAATGGACTGGAAATGGG GCCAGCAGGATGGAGGTGAGGGCGGTGTGGGCACCGTGGTGGAGCTTGGCCGCCATGGCAGCCCCTCGAC CCCCGACCGTACAGTTGTCGTTCAGTGGGACCAGGGCACACGTACCAACTATCGAGCTGGCTACCAAGGT GCCCATGACCTGTTGCTCTATGACAACGCCCAAATCGGTATCCGCCATCCCAACATCATCTGTGACTGTT GCAAGAAACATGGGCTTCGTGGCATGCGTTGGAAGTGCCGTGTCTGCTTTGACTATGATCTCTGCACGCA GTGCTACATGCACAACAAACATGACCTTACCCATGCCTTCGAGCGCTATGAGACATCTCACTCTCGCCCG GTTACGCTGAGTCCCCGACAGGGCCTCCCTCGAATCCCACTGAGGGGCATCTTTCAAGGAGCAAAAGTGG TACGAGGCCCTGACTGGGAATGGGGCTCACAAGATGGAGGGGAAGGCAAGACAGGCCGTGTGGTGGACAT CCGTGGCTGGGATGTGGAGACAGGCCGAAGTGTGGCCAGTGTGACATGGGCAGATGGAACCACAAATGTG TACCGAGTGGGCCACAAAGGCAAGGTGGATCTCAAATGTGTTGGTGAGGCAGCTGGCGGCTTTTACTACA AGGAGCACCTCCCGAAGCTTGGCAAGCCAGCAGAGCTACAGCGCAGGGTGAGTGCTGATGGCCAGCCCTT CCAGCGTGGGGACAAGGTCAAGTGTCTGCTGGACACAGATGTCCTAAGGGACATGCAAGAAGGCCATGGT GGCTGGAACCCTAGGATGGCAGAGATGGGCACCGTGCACCGCATCACAGACCGTGGGGACGTGCGTGTGC AGTTCAACCATGAAACCCGCTGGACCTTCCACCCTGGGGCTCTCACCAAGCACAACAGCTTCTGGGTAGG TGATGTGGTCCGGGTCATCGATGACCTTGACACTGTGAAGCGACTGCAAGCTGGACATGGAGAATGGACT GACGACATGGCCCCCGCCCTGGGCCGAGTGGGGAAAGTGGTGAAGGTGTTTGGAGATGGAAACTTGCGTG TGGCGGTTGGTGGCCAGCGGTGGACCTTCAGCCCCGCCTGCCTAGTGGCCTGCCGGCCTGAGGAGGATGC CAACCTGGGCGTGGCTGAGCGCGCCAGAGAGAACAAAAGCTCACTGAGTGTGGCCCTGGACAAGCTTCGG ACACAGAAGAGCGACCCAGAGCACCCAGGGAGGCTGGTAGTGGAGGCTGCACTGGGAAATGTAGCCCGGG CCCTGGACCTGCTTCGAAGGCATCCTGAGCAGGTGGACACCAAGAACCAGGGTAGGACTGCCCTGCAGGT GGCTGCTTACCTGGGCCAGGTAGAACTGGTACGGCTGCTGCTGCAGGCAAGAGCAAGTGTGGACCTGCTG GATGAGGAGGGCAACACTGCCCTTCACTACACAGCCATGGGGAACCAGCCTGAGGCCACAAGGCTGCTCC TGAGTGCCGGATGTGGGGTGGATGCTCAGAATGGCACACGCAGCACAGCCCTACACGTGGCTGTACAGAG GGGCTTCCTAGAGGTGGTAAAGATCCTGTGTGAACATGGTTGTGATGTCAACTTGCCGGATGCCCATGCA GACACACCCCTGCATTCTGCCATCTCTGCGGGTGCCGGTGCCAGCAGCATTGTTGAAGTCCTCACCGAGG TGCCTGGCATCGATGTCACTGCTACCAATAGCCAGGGCTTCACACTGCTGCACCATGCATCCCTCAAGGG CCATGTGCTAGCAGTTAGAAAGATTCTGGCAAGAGCACGGCAGCTGGTGGATGCCAAGAAGGAGGACGGC TTTACTGCGCTACATCTGGCAGCTCTTAACAACCACCGTGAGGTGGCCCAGGTCCTAATCCGAGAGGGCC GCTGCGATGTGAATGTGCGAAACAGGAAGCTTCAATCCCCACTGCATCTGGCTGTGCAGCAGGCCCACCT GGGGCTCGTACCGCTGCTGGTGGACGCTGGCTGCAATGTCAACACTGAGGATGAGGAGGGTGACACAGCC CTGCATGTGGCACTACAGCGTCATCAGCTGCTGCCCCTGGTGGCTGACAGGGCTGGGGGAGACCCAGGGC CCTTGCAGCTGCTGTCAAGGTTACAGGCCTCAGGCCTCCCAGGCAGCACAGAGTTGACTGTAGGCGCCGC AGTGGCCTGCTTCCTGGCATTGGAGGGTGCTGATGTGAGCTACGCAAACCACCGCGGCCGGAGCCCACTG GACCTGGCCACAGAAGGCCGAGTGCTCAAGGCCTTGCAGGGCTGTGCCCAGCGCTTCAGGGAGCGACAGG CAGGTGGCGGTGGGGGTGTGCCCCCGGGCCCCCGGCATGTGCTGAGTACTCCTAACACCGTGACGAACCT GCATGTGTCTGGCACAGCAGGGCCAGAAGCTGCCGAGTGCCTGGTGTGCTCGGAGCTGGCATTGCTAGTT CTGTTCTCACCGTGTCAGCACCGCACAGTGTGTGAGGAGTGCGCACGAAGGATGAAGAAGTGTATCAGGT GCCAGGTGATCATCAGCAAGAAGCTTCGCCCAGATGGTTCAGAGGTGGTTAATGCCATCCAGGTGCCCGG CCCGCCTCGGCAACTGGTAGAGGAGCTACAGAGTCGCTACAGGCAGATGGAGGAGCGCATCACCTGCCCC ATCTGCATCGACAGCCACATCCGCCTGGTGTTCCAGTGCGGCCATGGAGCATGTGCGCCCTGTGGCGCTG CCCTTAACGCCTGCCCCATTTGCCGCCAGCCCATCCGTGACCGCATTCAGATCTTCGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217359 representing NM_001005901 Red=Cloning site Green=Tags(s) MDLDPHAGVQVGMRVVRGMDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQG AHDLLLYDNAQIGIRHPNIICDCCKKHGLRGMRWKCRVCFDYDLCTQCYMHNKHDLTHAFERYETSHSRP VTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKTGRVVDIRGWDVETGRSVASVTWADGTTNV YRVGHKGKVDLKCVGEAAGGFYYKEHLPKLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLRDMQEGHG GWNPRMAEMGTVHRITDRGDVRVQFNHETRWTFHPGALTKHNSFWVGDVVRVIDDLDTVKRLQAGHGEWT DDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPACLVACRPEEDANLGVAERARENKSSLSVALDKLR TQKSDPEHPGRLVVEAALGNVARALDLLRRHPEQVDTKNQGRTALQVAAYLGQVELVRLLLQARASVDLL DEEGNTALHYTAMGNQPEATRLLLSAGCGVDAQNGTRSTALHVAVQRGFLEVVKILCEHGCDVNLPDAHA DTPLHSAISAGAGASSIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHVLAVRKILARARQLVDAKKEDG FTALHLAALNNHREVAQVLIREGRCDVNVRNRKLQSPLHLAVQQAHLGLVPLLVDAGCNVNTEDEEGDTA LHVALQRHQLLPLVADRAGGDPGPLQLLSRLQASGLPGSTELTVGAAVACFLALEGADVSYANHRGRSPL DLATEGRVLKALQGCAQRFRERQAGGGGGVPPGPRHVLSTPNTVTNLHVSGTAGPEAAECLVCSELALLV LFSPCQHRTVCEECARRMKKCIRCQVIISKKLRPDGSEVVNAIQVPGPPRQLVEELQSRYRQMEERITCP ICIDSHIRLVFQCGHGACAPCGAALNACPICRQPIRDRIQIFV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001005901
ORF Size	2859 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001005901.2, NP_001005901.2
RefSeq Size	3799 bp
RefSeq ORF	2862 bp
Locus ID	474147
MW	103.9 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.