Kcnq5 (NM_001134643) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Kcnq5l
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217357 representing NM_001134643 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCGCCACCACGCGGGAGGAGAGGAGGGCGGTGCCGCCGGGCTCTGGGTGAGGAGCGGCGCGGCGG CGGCGGCGGGTGGGGGGCGCCCGGGCAGCGGCATGAAGGATGTGGAGTCCGGCCGCGGCAGGGTGCTGCT GAACTCGGCGGCCGCCAGGGGCGACGGCCTGCTGCTGCTCGGCACCCGCGCGGCGGCGCTCGGCGGAGGC GGCGGCGGCTTGAGAGAGAGCCGCCGGGGCAAGCAGGGGGCCCGGATGAGCCTGCTGGGGAAGCCGCTCT CCTACACCAGCAGCCAGAGCTGTCGGCGCAACGTCAAGTACCGGAGGGTGCAGAACTATCTGTACAACGT GCTGGAGAGACCCCGCGGCTGGGCGTTCGTCTACCACGCGTTCGTTTTTCTCCTTGTGTTTGGTTGCTTG ATTTTGTCAGTGTTTTCTACCATCCCTGAGCACACAAAGTTGGCTTCAAGTTGCCTCTTAATTCTGGAGT TCGTGATGATCGTTGTCTTTGGCTTGGAGTTCATCATTCGAATCTGGTCTGCAGGTTGCTGTTGTCGTTA TAGAGGATGGCAAGGGAGACTGAGGTTTGCTCGAAAGCCTTTCTGTGTTATAGATACCATTGTTCTTATC GCTTCAATAGCAGTTGTTTCTGCAAAAACTCAGGGTAATATTTTCGCCACGTCAGCGCTCAGAAGTCTCC GGTTCCTACAGATCCTGCGTATGGTGCGCATGGACAGAAGGGGAGGGACCTGGAAGTTGCTGGGCTCCGT GGTTTACGCTCACAGCAAGGAATTAATCACAGCCTGGTACATTGGATTTCTGGTTCTTATTTTTTCATCC TTCCTTGTCTATCTTGTGGAAAAAGATGCCAATAAAGAGTTTTCAACATATGCGGATGCTCTCTGGTGGG GCACAATTACACTGACAACCATTGGCTATGGAGACAAAACACCCCTAACTTGGCTGGGAAGATTGCTCTC TGCAGGCTTCGCCCTTCTTGGTATTTCTTTCTTTGCACTTCCTGCTGGCATTCTTGGCTCAGGTTTTGCC TTAAAAGTACAGGAACAGCACCGCCAGAAGCATTTTGAGAAAAGAAGGAACCCAGCTGCCAACCTCATCC AGTGTGTCTGGCGTAGCTATGCAGCTGATGAGAAGTCGGTCTCCATCGCAACCTGGAAGCCACATCTGAA GGCCTTGCACACCTGCAGCCCTACCAAGAAAGAACAAGGGGAGGCAGCAAGCAGTAAGTTCTGTAGTAAT AAGCAGAAGTTCTTCAGAATGTACACCTCACGGAAGCAGAGTCAGAAGCTGAGCTTTAAGGAGCGAGTGC GCATGGCTAGCCCAAGAGGCCAGAGCATTAAGAGCAGACAAGCATCGGTAGGTGACCGGAGATCCCCAAG CACTGACATCACTGCTGAGGGCAGCCCCACCAAAGTGCAGAAGAGCTGGAGCTTCAACGACCGAACCCGC TTCAGGCCCTCACTACGCCTCAAGAGTTCCCAGCCGAAGACGGTGATAGACGCTGACACAGCCCTTGGCA TTGACGACGTATACGATGAGAAAGGATGCCAGTGTGACGTGTCCGTGGAGGACCTCACCCCACCACTCAA AACCGTCATCCGAGCCATCAGAATCATGAAGTTTCATGTTGCAAAGCGGAAGTTTAAGGAAACATTACGC CCATATGATGTAAAGGATGTCATTGAGCAATACTCTGCTGGCCATCTGGACATGCTATGTAGAATTAAAA GCCTTCAAACACGCGTTGATCAAATTCTTGGAAAAGGACAAATCACATCAGATAAAAAGAGCCGAGAGAA AATAACGGCAGAACACGAGACAACGACAGATGACCTCAGCATGCTGGGCCGAGTTGTGAAGGTTGAAAAA CAGGTACAATCCATTGAATCCAAGCTGGACTGCTTGCTGGATATCTACCAGCAGGTCCTCCGGAAAGGCT CTGCCTCGGCCCTCACTCTGACGTCCTTCCAGATCCCGCCCTTTGAATGTGAACAGACCTCTGACTATCA AAGTCCCGTGGAGAGCAAAGACCTGTCGAGCTCCGCGCAAAACAGTGGCTGCTTAAGCAGGTCAGCCAGT GCCAACATCTCAAGAGGCCTGCAGTTCATCCTAACGCCAAATGAGTTCAGTGCCCAGACTTTCTACGCGC TTAGCCCTACTATGCACAGCCAAACTACACAGGTGCCAATGAGTCAAAACGACGGCTCCTCCGTGGTGGC CACCAATAACCTTGCAAACCAAATAAGCGCCGCACCCAAGCCAGCAGCTCCAACAACTTTACAGATCCCA CCTCCTCTCTCAGCCATCAAGCATCTATCCAGGCCGGAACCCCTGCTGTCCAACCCCCCTGGCGTACACG AGAGTATTTCTGACGTCACCACCTGCCTTGTTGCCTCCAAGGAAAACGTTCAGTTCGCCCAGTCAAACCT GACCAAGGACCGTTCCCTGAGGAAAAGTTTTGACATGGGAGGGGAAACTCTGTTGTCCGTCCGCCCCATG GTGCCCAAGGACTTGGGCAAATCTCTGTCTGTACAAAACCTGATCAGATCGACAGAGGAACTGAACTTAC AGTTTTCGGGCAGTGAGTCAAGTGGCTCTCGAGGCAGCCAAGATTTTTATCCCAAGTGGAGAGAATCCAA ATTGTTTATAACTGATGAGGAGGTCGGTGCCGAGGAGACAGAAACAGATACTTTTGACGGCACCCCACCG CCTGCCGGGGAAGCTGCTTTCTCATCAGATTCTCTAAGGACTGGAAGGTCACGGTCATCTCAGAACATTT GTAAGACGGGAGACAGTACGGATGCCCTCAGTTTGCCTCACGTCAAACTGAAC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217357 representing NM_001134643 Red=Cloning site Green=Tags(s) MPRHHAGGEEGGAAGLWVRSGAAAAAGGGRPGSGMKDVESGRGRVLLNSAAARGDGLLLLGTRAAALGGG GGGLRESRRGKQGARMSLLGKPLSYTSSQSCRRNVKYRRVQNYLYNVLERPRGWAFVYHAFVFLLVFGCL ILSVFSTIPEHTKLASSCLLILEFVMIVVFGLEFIIRIWSAGCCCRYRGWQGRLRFARKPFCVIDTIVLI ASIAVVSAKTQGNIFATSALRSLRFLQILRMVRMDRRGGTWKLLGSVVYAHSKELITAWYIGFLVLIFSS FLVYLVEKDANKEFSTYADALWWGTITLTTIGYGDKTPLTWLGRLLSAGFALLGISFFALPAGILGSGFA LKVQEQHRQKHFEKRRNPAANLIQCVWRSYAADEKSVSIATWKPHLKALHTCSPTKKEQGEAASSKFCSN KQKFFRMYTSRKQSQKLSFKERVRMASPRGQSIKSRQASVGDRRSPSTDITAEGSPTKVQKSWSFNDRTR FRPSLRLKSSQPKTVIDADTALGIDDVYDEKGCQCDVSVEDLTPPLKTVIRAIRIMKFHVAKRKFKETLR PYDVKDVIEQYSAGHLDMLCRIKSLQTRVDQILGKGQITSDKKSREKITAEHETTTDDLSMLGRVVKVEK QVQSIESKLDCLLDIYQQVLRKGSASALTLTSFQIPPFECEQTSDYQSPVESKDLSSSAQNSGCLSRSAS ANISRGLQFILTPNEFSAQTFYALSPTMHSQTTQVPMSQNDGSSVVATNNLANQISAAPKPAAPTTLQIP PPLSAIKHLSRPEPLLSNPPGVHESISDVTTCLVASKENVQFAQSNLTKDRSLRKSFDMGGETLLSVRPM VPKDLGKSLSVQNLIRSTEELNLQFSGSESSGSRGSQDFYPKWRESKLFITDEEVGAEETETDTFDGTPP PAGEAAFSSDSLRTGRSRSSQNICKTGDSTDALSLPHVKLN SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001134643
ORF Size	2853 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001134643.2, NP_001128115.2
RefSeq Size	3807 bp
RefSeq ORF	2856 bp
Locus ID	259273
MW	104.7 kDa

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