Iqsec2 (NM_001277386) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1562109
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217356 representing NM_001277386 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACCCCCCTGGAAGAGCAGCAGGTCCACAGCGTCTCATACTCTACACCAGTATTGCTGTCCTACTC AGGTCCTTGACTCCATGAAGCTTACCCCCTCAGGCAGGCTGGCAGAGAGCAGTGTGGAGGGTGATGCCCC AGGCAGTGACCTGAGCACAGCGGTTGATAGTCCTGGGAGCCAACCCCCCTACCGGCTGAGCCAACTGCCC CCAACCAGCAGCCACATGGGGGGCCCCCCTGCTGGCGTGGGCCTACCTTGGTCTCAGAGGGCTCGCCTCC AGCCAGCCAGTGTCGCCCTGAGGAAGCAGGAGGAGGAGGAGATAAAGCGCTCCAAGGCCCTGTCAGACAG CTATGAACTCTCCACAGACCTACAGGACAAGAAGGTGGAAATGCTGGAGAGGAAGTATGGGGGCTCCTTC CTGAGCCGCAGGGCTGCCAGGACCATCCAGACAGCCTTTCGCCAGTATCGTATGAATAAGAACTTTGAGC GACTGCGCAGCTCGGCTTCAGAGAGCCGCATGTCCCGCCGCATCATCTTGTCCAACATGCGGATGCAGTT CTCCTTTGAGGAGTACGAGAAAGCACAGAACCCTGCATACTTCGAAGGCAAGCCTGCCTCATTGGATGAG GGTGCAATGGCTGGAGCCCGGAGCCACCGGCTTGAACGGGGCCTCCCCTATGGAGGCTCCTGTGGTGGGG GCATCGATGGTGGTGGAAGTTCAGTCACCACATCTGGAGAGTTTTCTAATGACATCACAGAGCTCGAGGA CTCCTTCTCCAAACAGGTAAAGTCTCTGGCTGAATCCATTGATGAGGCCTTGAACTGCCACCCATCAGGG CCTATGTCTGAGGAGCCAGGGTCAGCCCAACTGGAGAAGCGGGAATCAAAGGAACAGCAAGAAGACAGCT CAGCCACATCCTTCAGTGACCTCCCCCTCTACTTGGATGACCCAGTTCCTCCTCCATCACCTGAGCGACT GCCCAGCACAGAGCCCCCACCCCAAGGCCGCCCTGAGTTCTGGGCACCAGCTCCCCTCCCACCAGTTCCT CCACCAATGCCACCAGGGACCCGGGAAGATGGTAGCCGTGAGGAAGGCACTCGAAGGGGTCCTGGGTGCT TGGAGTGCCGGGATTTCCGGCTCCGGGCTGCACACCTCCCCCTCCTCACCATTGAGCCTCCCAGTGACAG TTCTGTAGACCTGAGTGACCGCTCAGATCGCGGCTCTGTCCACCGCCAGCTGGTGTATGAGGCTGATGGC TGCAGCCCCCATGGGACCCTGAAGCACAAGGGGCCACCAGGCAGGGCCCCAATCCCACACCGCCACTACC CTGCCCCTGAGGGTCCAGCTCCAGCCCCACCAGGGCCCCTGCCACCAGCCCCCAATAGTGGTACTGGGCC CAGTGGTGTGGCTGGAGGTCGGAGGTTGGGAAAGTGTGAGGCAGCAGGTGAGAACTCTGATGGTGGAGAC AATGAGAGCCTTGAGAGCTCCAGCAACTCTAATGAGACCATCAACTGCAGCTCTGGCTCCTCCTCTCGGG ATAGCCTGAGGGAACCTCCAGCCACTGGCCTGTGCAAGCAGACTTACCAGCGGGAGACAAGGCACAGCTG GGACTCGCCAGCCTTCAACAATGACGTGGTACAAAGAAGGCATTACAGAATTGGCCTCAACCTCTTCAAT AAGAAGCCAGAGAAGGGTATCCAGTATTTGATCGAGCGAGGCTTCCTGTCAGACACTCCTGTGGGGGTGG CTCACTTCATCCTGGAGCGGAAAGGCCTGAGTCGGCAGATGATAGGGGAATTCCTGGGGAATCGACAGAA GCAGTTCAACAGAGATGTGTTGGACTGTGTGGTGGATGAGATGGACTTCTCCTCTATGGATTTGGACGAT GCACTCAGGAAGTTCCAATCCCATATCCGAGTTCAGGGTGAGGCCCAGAAAGTGGAGCGACTCATTGAAG CTTTCAGCCAGCGGTACTGTGTCTGTAACCCTGCCCTCGTACGACAGTTCCGGAACCCAGACACTATCTT CATCCTTGCTTTTGCCATCATCCTCCTCAATACCGATATGTACAGCCCCAGTGTCAAGGCTGAACGCAAG ATGAAGCTGGATGACTTCATTAAGAACCTGAGAGGAGTTGACAATGGTGAAGACATCCCCCGAGACCTCC TAGTAGGCATCTACCAGCGCATCCAAGGTCGTGAACTAAGGACCAACGATGACCATGTGTCCCAGGTGCA GGCTGTGGAGCGCATGATTGTTGGCAAGAAACCGGTCCTGTCGCTCCCTCACCGTCGACTGGTTTGCTGC TGCCAGCTCTATGAGGTGCCAGATCCAAATCGCCCCCAAAGGCTGGGGCTGCATCAGCGGGAGGTCTTCC TCTTCAATGACCTCCTTGTGGTCACCAAAATTTTCCAGAAGAAGAAGATCTTGGTGACATACAGCTTCCG TCAGTCCTTTCCCCTGGTGGAAATGCATATGCAGCTCTTCCAGAATTCATATTATCAGTTTGGAATCAAG TTACTATCTGCGGTACCTGGAGGGGAACGAAAAGTTCTCATTATCTTTAATGCTCCTAGCCTCCAAGACA GGCTACGCTTTACCTCTGACCTGCGGGAGTCCATTGCTGAGGTGCAGGAGATGGAGAAGTACCGTGTGGA ATCGGAGCTGGAGAAGCAGAAAGGTATGATGCGGCCTAATGCCTCACAACCTGGAGGAGCCAAGGACTCA GTGAATGGGACTCTGGCCCGCAGTAGTCTGGAGGACACTTATGGGGCAGGCGATGGGCTCAAGCGGGGTG CACTCAGTAGTTCCCTGCGAGACCTCTCTGATGCAGGGGTCTGTTAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217356 representing NM_001277386 Red=Cloning site Green=Tags(s) MEPPWKSSRSTASHTLHQYCCPTQVLDSMKLTPSGRLAESSVEGDAPGSDLSTAVDSPGSQPPYRLSQLP PTSSHMGGPPAGVGLPWSQRARLQPASVALRKQEEEEIKRSKALSDSYELSTDLQDKKVEMLERKYGGSF LSRRAARTIQTAFRQYRMNKNFERLRSSASESRMSRRIILSNMRMQFSFEEYEKAQNPAYFEGKPASLDE GAMAGARSHRLERGLPYGGSCGGGIDGGGSSVTTSGEFSNDITELEDSFSKQVKSLAESIDEALNCHPSG PMSEEPGSAQLEKRESKEQQEDSSATSFSDLPLYLDDPVPPPSPERLPSTEPPPQGRPEFWAPAPLPPVP PPMPPGTREDGSREEGTRRGPGCLECRDFRLRAAHLPLLTIEPPSDSSVDLSDRSDRGSVHRQLVYEADG CSPHGTLKHKGPPGRAPIPHRHYPAPEGPAPAPPGPLPPAPNSGTGPSGVAGGRRLGKCEAAGENSDGGD NESLESSSNSNETINCSSGSSSRDSLREPPATGLCKQTYQRETRHSWDSPAFNNDVVQRRHYRIGLNLFN KKPEKGIQYLIERGFLSDTPVGVAHFILERKGLSRQMIGEFLGNRQKQFNRDVLDCVVDEMDFSSMDLDD ALRKFQSHIRVQGEAQKVERLIEAFSQRYCVCNPALVRQFRNPDTIFILAFAIILLNTDMYSPSVKAERK MKLDDFIKNLRGVDNGEDIPRDLLVGIYQRIQGRELRTNDDHVSQVQAVERMIVGKKPVLSLPHRRLVCC CQLYEVPDPNRPQRLGLHQREVFLFNDLLVVTKIFQKKKILVTYSFRQSFPLVEMHMQLFQNSYYQFGIK LLSAVPGGERKVLIIFNAPSLQDRLRFTSDLRESIAEVQEMEKYRVESELEKQKGMMRPNASQPGGAKDS VNGTLARSSLEDTYGAGDGLKRGALSSSLRDLSDAGVCY TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001277386
ORF Size	2847 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001277386.1, NP_001264315.1
RefSeq Size	4547 bp
RefSeq ORF	2850 bp
Locus ID	685244
MW	105.2 kDa

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