Icam5 (NM_001172079) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217321 representing NM_001172079 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGGGCCTTCACCAGGGCTGCGCCGAACGCTCCTCGGCCTCTGGGCTGCCCTGGGCCTGGGGATCC TAGGCATCTCAGCGGTCGCGCTAGAACCTTTCTGGGCGGACCTTCAGCCCCGCGTGGCGCTCGTGGAGCG CGGGGGCTCGCTGTGGCTCAACTGCAGCACTAACTGTCCGAGGCCGGAGCGCGGTGGCCTGGAGACCTCG CTACGCCGAAACGGGACCCAGAGGGGTCTGCGCTGGCTGGCTCGACAGCTGGTGGACATCCGAGAGCCTG AAACCCAGCCGGTCTGCTTCTTCCGCTGCGCGCGCCGCACACTCCAAGCGCGTGGGCTCATCCGAACTTT CCAGCGACCGGATCGGGTAGAGCTAGTGCCTCTGCCTCCTTGGCAGCCTGTAGGTGAGAACTTCACCTTG AGCTGCAGGGTCCCGGGGGCAGGACCCCGAGCGAGCCTCACATTGACCTTGCTGCGAGGCGGCCAGGAGC TGATTCGCCGAAGTTTCGTAGGCGAGCCACCCCGAGCTCGGGGTGCGATGCTCACCGCCACGGTCCTGGC GCGCAGAGAGGATCACAGGGCCAATTTCTCATGCCTCGCGGAGCTTGACCTGCGGCCACACGGCTTGGGA CTGTTTGCAAACAGCTCAGCCCCCAGACAGCTCCGCACGTTTGCCATGCCTCCACTTTCCCCGAGCCTTA TTGCCCCACGATTCTTAGAAGTGGGCTCAGAAAGGCCGGTGACTTGCACTTTGGATGGACTGTTTCCTGC CCCAGAAGCCGGGGTTTACCTCTCTCTGGGAGATCAGAGGCTTCATCCTAATGTGACCCTCGACGGGGAG AGCCTTGTGGCCACTGCCACAGCTACAGCAAGTGAAGAACAGGAAGGCACCAAACAGCTGATGTGCATCG TGACCCTCGGGGGCGAAAGCAGGGAGACCCAGGAAAACCTGACTGTCTACAGCTTCCCGGCTCCTCTTCT GACTTTAAGTGAGCCAGAAGCCCCCGAGGGAAAGATGGTGACCGTAAGCTGCTGGGCAGGGGCCCGAGCC CTTGTCACCTTGGAGGGAATTCCAGCTGCGGTCCCTGGGCAGCCCGCTGAGCTCCAGTTAAATGTCACAA AGAATGACGACAAGCGGGGCTTCTTCTGCGACGCTGCCCTCGATGTGGACGGGGAAACTCTGAGAAAGAA CCAGAGCTCTGAGCTTCGTGTTCTGTACGCACCTCGGCTGGATGACTTGGACTGTCCCAGGAGCTGGACG TGGCCAGAGGGTCCAGAGCAGACCCTCCACTGCGAGGCCCGTGGAAACCCTGAGCCCTCCGTGCACTGTG CAAGGCCTGACGGTGGGGCGGTGCTAGCGCTGGGCCTGTTGGGTCCAGTGACCCGTGCCCTCGCGGGCAC TTACCGATGTACAGCAATCAATGGGCAAGGCCAGGCGGTCAAGGATGTGACCCTGACTGTGGAATATGCC CCAGCGCTGGACAGTGTAGGCTGCCCAGAACGTATTACTTGGCTGGAGGGGACAGAGGCATCGCTTAGCT GTGTGGCACACGGGGTCCCACCACCTAGCGTGAGCTGTGTGCGCTCTGGAAAGGAGGAAGTCATGGAAGG GCCCCTGCGTGTGGCCCGGGAGCACGCTGGCACTTACCGATGCGAAGCCATCAACGCCAGGGGATCAGCG GCCAAAAATGTGGCTGTCACGGTGGAATATGGTCCCAGTTTTGAGGAGTTGGGCTGCCCCAGCAACTGGA CTTGGGTAGAAGGATCTGGAAAACTGTTTTCCTGTGAAGTTGATGGGAAGCCGGAACCACGCGTGGAGTG CGTGGGCTCGGAGGGTGCAAGCGAAGGGGTAGTGTTGCCCCTGGTGTCCTCGAACTCTGGTTCCAGAAAC TCTATGACTCCTGGTAACCTGTCACCGGGTATTTACCTCTGCAACGCCACCAACCGGCATGGCTCCACAG TCAAAACAGTCGTCGTGAGCGCGGAATCACCGCCACAGATGGATGAATCCAGTTGCCCGAGTCACCAGAC ATGGCTGGAAGGAGCCGAGGCTACTGCGCTGGCCTGCAGTGCCAGAGGCCGCCCCTCTCCACGCGTGCGC TGTTCCAGGGAAGGTGCAGCCAGGCTGGAGAGGCTACAGGTGTCCCGAGAGGATGCGGGGACCTACCTGT GTGTGGCTACCAACGCGCATGGCACGGATTCACGGACCGTCACTGTGGGTGTGGAATACCGGCCTGTGGT GGCTGAGCTGGCAGCCTCGCCCCCAAGCGTGCGGCCTGGCGGAAACTTCACTCTGACCTGCCGTGCAGAG GCCTGGCCTCCAGCCCAGATCAGCTGGCGCGCGCCCCCGGGAGCTCTCAACCTCGGTCTCTCCAGCAACA ACAGCACGCTGAGCGTGGCGGGTGCCATGGGCAGCCATGGTGGCGAGTATGAGTGCGCAGCCACCAATGC GCATGGGCGCCACGCACGGCGCATCACGGTGCGCGTGGCCGGTCCATGGCTGTGGGTCGCTGTGGGCGGT GCGGCAGGGGGCGCGGCGCTGCTGGCCGCAGGGGCCGGCCTGGCCTTCTACGTGCAGTCCACCGCTTGCA AGAAGGGAGAGTACAACGTCCAGGAGGCTGAGAGCTCAGGCGAGGCGGTGTGTCTCAATGGCGCGGGCGG GACACCGGGTGCAGAAGGCGGAGCAGAGACCCCCGGCACTGCCGAGTCACCTGCAGATGGCGAGGTTTTC GCCATCCAGCTGACATCTTCC ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA TTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217321 representing NM_001172079 Red=Cloning site Green=Tags(s) MPGPSPGLRRTLLGLWAALGLGILGISAVALEPFWADLQPRVALVERGGSLWLNCSTNCPRPERGGLETS LRRNGTQRGLRWLARQLVDIREPETQPVCFFRCARRTLQARGLIRTFQRPDRVELVPLPPWQPVGENFTL SCRVPGAGPRASLTLTLLRGGQELIRRSFVGEPPRARGAMLTATVLARREDHRANFSCLAELDLRPHGLG LFANSSAPRQLRTFAMPPLSPSLIAPRFLEVGSERPVTCTLDGLFPAPEAGVYLSLGDQRLHPNVTLDGE SLVATATATASEEQEGTKQLMCIVTLGGESRETQENLTVYSFPAPLLTLSEPEAPEGKMVTVSCWAGARA LVTLEGIPAAVPGQPAELQLNVTKNDDKRGFFCDAALDVDGETLRKNQSSELRVLYAPRLDDLDCPRSWT WPEGPEQTLHCEARGNPEPSVHCARPDGGAVLALGLLGPVTRALAGTYRCTAINGQGQAVKDVTLTVEYA PALDSVGCPERITWLEGTEASLSCVAHGVPPPSVSCVRSGKEEVMEGPLRVAREHAGTYRCEAINARGSA AKNVAVTVEYGPSFEELGCPSNWTWVEGSGKLFSCEVDGKPEPRVECVGSEGASEGVVLPLVSSNSGSRN SMTPGNLSPGIYLCNATNRHGSTVKTVVVSAESPPQMDESSCPSHQTWLEGAEATALACSARGRPSPRVR CSREGAARLERLQVSREDAGTYLCVATNAHGTDSRTVTVGVEYRPVVAELAASPPSVRPGGNFTLTCRAE AWPPAQISWRAPPGALNLGLSSNNSTLSVAGAMGSHGGEYECAATNAHGRHARRITVRVAGPWLWVAVGG AAGGAALLAAGAGLAFYVQSTACKKGEYNVQEAESSGEAVCLNGAGGTPGAEGGAETPGTAESPADGEVF AIQLTSS TRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-NotI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001172079
ORF Size	2751 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001172079.1, NP_001165550.1
RefSeq Size	3117 bp
RefSeq ORF	2754 bp
Locus ID	313785
MW	96.9 kDa

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