Garem2 (NM_001191874) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Fam59b; Gareml
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217293 representing NM_001191874 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAAGCTGGCGGCCGGGCTTGCCGGGCTGCGCTGGAGCATGGGCGCTTTCCCGCTGGACCTCATCG TCAGCCGCTGTCGCCTGCCCACGCTCGCCTGCCTCGGGCCAGGGGAGTATGCTGAGGGTGTCAGTGAACG AGATATCCTGCTGATCCACTCCTGCCGCCAGTGGACCACAGTGACCGCCCATACCCTGGAGGAGGGCCAC TACGTCATTGGACCCAAGATTGACATCCCCCTGCAGTACCCAGGCAAATTCAAGCTCCTGGAGCAGGCTC GGGATGTGCGGGAGCCGGTGAGATACTTCAGCAGTGTGGAGGAGGTGGCCAGTGTCTTCCCTGACCGAAT CTTTGTGATGGAAGCCATCACCTTCAGTGTCAAGGTGGTGTCAGGCGAGTTCAGCGAGGACAGCGAGGTG TACAACTTCACACTGCACGCAGGCGACGAGCTCACTCTCATGGGTCAGGCCGAGATTCTGTGCGCCAAGA CAACCAAAGAGCGCTCGCGCTTCACCACGCTCCTGCGCAAGTTGGGCCGTGCAGGGGCCTTGGCTGGAAT CGGCGGTCCCGGGAGTATGGGGGCCACAGGAGGTGGCGGCGGGGCCGCCAGGCCCGTCAGAGGCAAGATG CCCTGTCTCATCTGCATGAACCACCGCACCAACGAGAGCCTGAGCCTGCCCTTCCAATGCCAGGGCCGCT TTAGCACGCGCAGCCCTCTGGAGCTACAAATGCAGGAGGGCGAGCACACGGTGCGCGCCATCATCGAGCG CGTGAGGCTTCCGGTGAACGTGCTGGTACCCAGCCGGCCGCCGCGCAACCCTTACGACCTACACCCTGTG CGGGAGGGCCACTGCTATAAGCTGGTCAGCATCATCTCCAAGACCGTGGTGCTGGGGCTGGCGCTGCGCC GTGAGGGTCCGGCGCCACTGCACTTCCTGCTGCTCACCGACACGCCGCGCTTCACTCTGCCGCAGGGTCT GCTGGCCGGGGACCCGCGCGTCGAGCGCCTGGTGCGCGACAGCGCCTCCTACTGTCGCGAGCGCTTCGAT CCGGACGAGTACTCCACTGCTGTGCGCGAGGCGCCCGCGGAGCTCGCGGACGATTGCGCCAGTCCGCGCC GTGCGCGCCTCTGCCTGCCCGCGCCGCGTGTTCCCGGGTCTGTCCGTGTGCCTGGACCGCCGGGTCGCCT GGGTCCTGCCCTGCCAGCACCGAGCGACAGCGACCAGGAGTACGTGAGCCCTGATTGGGCGGGTGCGCCC GAGCCCGCGGCCGCGTGCGCCGAGATCCCCTACGAGGAGCTGTGGGTGCACCAAGGCCCCGAAAGCCTCG CCGACGCCCGTGCACGGCCGCTGACAGGGCCGGATCTCATCTCTTTTGGGACCGTGGGGCCCCCACAGCA TGAACCAGAGGCACCACCGCCTCCTGTCCCCCCCAAATCTGAGGCGGTGAAGGAAGAGTGCCGCCTGCTT CACGCCCCGCCTGTGCCTCCTAGGGGTGGCAGCAGCTGCAGTAGGCTCACAGGCAGCCCTCCAGTGCCGC CCCGCTTCCCCAAGCTGCAGTCTGTCCACTCACCGAGCTCCAGTCTCTCGTACTATTCCTCTGGCCTGCA GGATGGGGCAGGATCCCGCAGTGGCAGTGGTTCCCCGTCACCGGATGCATACTCTCTCTATTGCTACCCT TGCACCTGGGGAGATTGTAAAGCCAGCGAGTCCTCTAGTCGCCCGCCACCAGGACCCCTACCTTCTACCA CCACCCAGCCGCCCAGTCAGGCCTCAAGAGCCCTAGCAGAGCCTTTGAGTGGTCGAACCGCCTCCCTCTT GGGAGCTGACACCCCAGCGGTTAAGAACTACCATAGCTGCCCGCCTCTGTTTAAACCCTCACGTCCCCAG AAGTCCCTTGCTCCCTTTGGTGCACTCAACCCATTCTCTGGGCCTGCCCACCCTTCAGGTGCTCCAGCGG CCTCCTCCTCTGGGTCCACATCCACTTCGGGTGCCCTGGCTACCTCCAGCCCCACACACTCCCCAGGCCC AGGCCCACAGGGCCAAGGTTACTCAGCTGCTCCCTCCTCCTCCTTGTCCTCCTCTGAGTGGCAGGAGCCA GCGCTGGAGCCCCTGGACCCGTTTGAGCTGGGGCAGGCCAGTCCTCCTGAGCTGGAGCTGCTCCGCTGCC AGGAGCCCAGAGCTTCGGGGACACCTGGGTCTGGACATTGCCTTTCCCCACTTGGTCCGCCCAAGGCCTT AGAGCCGGAAGGTTTGGTGCTGAGGCAGGTCCCTGCCTCACTGTCACCAGCTGCCTTGCAGGGGCCCGAG GCAGGAGGAACCCTCCTCTTTCTTACCCAAGGGTGCCTGGAAGGGCCTCCTGGCAGCCCACGGGAGGGCG TCCCAGGTGCCGGAGTCCGGGATGCTTCCTCCTGGCAGCCACCAGCAGACTTATCTGCCCTCTCCGTGGA AGAGGTCTCACGGAGCCTGCGCTTCATCGGGCTCTCAGAGGACGTGGTGAGCTTCTTTGCCCGAGAACGC ATTGACGGAAGCATCTTTGTGCAGCTCAGTGAGGACATCTTGGCTGATGACTTTCATCTTACCAAGCTTC AGGTCAAGAAGATCATGCAGTTCATCAAAGGTTGGAGACCCAAGATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217293 representing NM_001191874 Red=Cloning site Green=Tags(s) MEKLAAGLAGLRWSMGAFPLDLIVSRCRLPTLACLGPGEYAEGVSERDILLIHSCRQWTTVTAHTLEEGH YVIGPKIDIPLQYPGKFKLLEQARDVREPVRYFSSVEEVASVFPDRIFVMEAITFSVKVVSGEFSEDSEV YNFTLHAGDELTLMGQAEILCAKTTKERSRFTTLLRKLGRAGALAGIGGPGSMGATGGGGGAARPVRGKM PCLICMNHRTNESLSLPFQCQGRFSTRSPLELQMQEGEHTVRAIIERVRLPVNVLVPSRPPRNPYDLHPV REGHCYKLVSIISKTVVLGLALRREGPAPLHFLLLTDTPRFTLPQGLLAGDPRVERLVRDSASYCRERFD PDEYSTAVREAPAELADDCASPRRARLCLPAPRVPGSVRVPGPPGRLGPALPAPSDSDQEYVSPDWAGAP EPAAACAEIPYEELWVHQGPESLADARARPLTGPDLISFGTVGPPQHEPEAPPPPVPPKSEAVKEECRLL HAPPVPPRGGSSCSRLTGSPPVPPRFPKLQSVHSPSSSLSYYSSGLQDGAGSRSGSGSPSPDAYSLYCYP CTWGDCKASESSSRPPPGPLPSTTTQPPSQASRALAEPLSGRTASLLGADTPAVKNYHSCPPLFKPSRPQ KSLAPFGALNPFSGPAHPSGAPAASSSGSTSTSGALATSSPTHSPGPGPQGQGYSAAPSSSLSSSEWQEP ALEPLDPFELGQASPPELELLRCQEPRASGTPGSGHCLSPLGPPKALEPEGLVLRQVPASLSPAALQGPE AGGTLLFLTQGCLEGPPGSPREGVPGAGVRDASSWQPPADLSALSVEEVSRSLRFIGLSEDVVSFFARER IDGSIFVQLSEDILADDFHLTKLQVKKIMQFIKGWRPKI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191874
ORF Size	2637 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191874.1, NP_001178803.1
RefSeq Size	2662 bp
RefSeq ORF	2640 bp
Locus ID	362801
MW	93.5 kDa

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