Pik3r5 (NM_001191923) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1563261
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217284 representing NM_001191923 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCCAGCAGCCACAACGTGCACAGAGGACCGCATCCAGCACGCTCTGGAGAGATGCTTGCATGGAC TGAGCCTCGGACGCCGTTCTGCTCCCTGGTCAGCCGGGCTGTGTCTGAACTGCTGGAGCCTACAGGAGCT GGTCAGCAGGGACCCGGGCCACTTCCTCATCCTCCTGGAACAGATCCTGCAGAAGACCCAAGAGGTCCAA GAGAAGGGCACCTATGACCTCCTCGCCCCTCTGGCTCTGCTCTTCTATTCCACTGTCCTCTGCACTCCAC ACTTCCCACCAGACTCAGACCTCCTGCTGAAGGCAGCCAGCACCTACCACTGCTTCCTGACCTGGCCGGT TCCCTACTGCAGCATCTGCCGAGAGATGCTCACCTTCATCGATGCTGAGCTCAAGGCCCCAGGAATCTCC TACCAGCGGCTGGTGAGGGCTGAACAGGGCCTGCCCATCAGGAGCCACCGAAGTTCCACTGTCACTGTGC TGTTGCTGAACCCGGTAGAGGTACAGGCTGAGTTCCTCGCTGTGGCAGACAAGCTGAGCACACCGGGACA GTCACCCCATGGCGCCTACACCACCCTGCTCCTGCATGCCTTCCAGGCCACCTTTGGGACCCACTGTGAC CTTCCTAAACTGCACCGCAAACTTCAGTCCAAGACGCTAGAAGAGCTCGAGGACATCTTCACTGAGACTA CAGAAGCCCAGGAGCTGGCATCTGGCATTGGGGATGTAGCAGAGGCCCGTCAGTGGCTCCGGACCAAGCT GCAGGCAGTGGGAGAAAAAGCCGGCTTCCCCGGGATCTTAGACACTGCAAGCCCTGGGAAACTCCACACG ATTCCCATCCCTGTCGCCAGATGCTACACTTACAGCTGGAATCAGGACAGCTTTGACATCCTACAGGAAG TCCTTCTCAAGGAGCAGGAGTTGCTGCAGCCAGGGATCCTGGGAGATGATGAAGAGGAGGAAGAGGAGGA CTTGGAGATTGACAGGCATATTGCTGAGAGGGACTCCCTGCTCTCCACAGGCTCTCTGGTGTCTCACGAC TCCGAACTGTTGCTTACCTCCTCTCAGGCCTCAGGACCAGTGCTGTCCCGCCAGGTGCTGACCACCTTTG TCTCAGGCCTCTCGGATGGCATGGACAGTGGCTACGTTGAGGACAGTGAGGAAAGCTCCTCAGAGTGGCC CAAGAAGCCGGGCAGCCAGCAGCGCCAGGGTCACCGCAGGCCTAGGCAGAAGTTCAACAGGTTCTATAAA CTTTTAAAGAGCACCAGCCAGTTGGTCCTGAGGAGGGACTCTCGGAGTCTGGAATGCAGCCCAGACCCAA CCCTGCCTCTGCGGCGGGCAGGGAGCCTCAGCAGCCCCTTGGACTTTCCGACCCAGCTCCCTTCCAGGGC TCAGCGCTCACGGTCCCTACCCCAGCCCAAGCTCAGCACCCAACTACCCAGCTGGCTTCTGGCACCACCC TCACACCACCAGCGCCGCCGTCCCTTCCTCAGCGGGGATGAGGACCCCAAGGCTTCCACACTACGGGTTG TGGTCTTTGGCTCTGACCGGATCTCAGGGAAGGTGGCCCGGGCCTATAGCAAGCTCAGGCGGCTGGAGAA CAGTCACCCGATCCTCACCCGGTTCTTCAAGCTTCAGTTCTTCTACGTACCTGTGAAGAGAAGCCACGGA GCCAGCTCTAGTGCCTGCCTATCCTCTCTGAACCAGGCATCTCCACTCCTTCCAGACTCCTTGAAGCACC CCAGCCCTGCAGACCTTGGCAAGGCCCCATGGGAGGACAGTACCAATGACATCTCTCACTACCTCGGTAT GCTTGACCCTTGGTATGAACGCAATGTGTTGGGCCTCATGCATCTGCCTCCTGAAGTCCTGTGTCAGCAG TCCCTCAAGGCAGAATCTCGGCCCCTGGAGGGCTCGGCCACCCAGCTGCCCATCCTGGCCGATATGTTAC TCTACTACTGCCGCTTTGCAGCCCGCCCAGTGTTGCTGCAGGTTTATCAGACAGAGCTGACCTTCGTCAC CGGGGAGAAGACCACGGAGATCTTTATCCAGTCTCTGGAGCTGGGTCACTCTGCGACCACTCGTGCTATC AAGGCTTCAGGTCCGGGAAGCAAAAGGCTGGGCATTGGCGATGACCGGGAGGCCGTCCCTCTAACACTAC AGATTATTTACAGCAAGGGAGCCATCAGTGGGCGAAGCCGCTGGAGCAACCTAGAGAAGGTCTGCACCTC TGTTAACCTCAGCAAGGCCTGCCAGAAGCCGGAGGAACTAGATTCCAGCATGGAGGCGCTGACGCTAACC CTGACAGAAGTGGTGAAGAGGCAGAACCCTAAATCCAAAAAGGGCTTTAACCAGATCAGTACATCCTACA TCAAAGTGGACAAGGTGCAGATCATCAGCTCCAGCAGCTGCCCCTTTGCTGTGTGTCTAGACCAGGATGA GAGGAAGATCCTGCAGAGTGTGGTCAGGTGTGAAGTGTCACCCTGCTACAAGCCAGAGAAGAGCAGCCTG CCACCTGAGGGGTCCCTCAGCCAACCAGCAGAGGCTGGGTCTGACCTGTGCTCCCTTCTCTGCCTTCCCA TCATGACTTTCAGTGGTGCTCTGCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217284 representing NM_001191923 Red=Cloning site Green=Tags(s) MQPAATTCTEDRIQHALERCLHGLSLGRRSAPWSAGLCLNCWSLQELVSRDPGHFLILLEQILQKTQEVQ EKGTYDLLAPLALLFYSTVLCTPHFPPDSDLLLKAASTYHCFLTWPVPYCSICREMLTFIDAELKAPGIS YQRLVRAEQGLPIRSHRSSTVTVLLLNPVEVQAEFLAVADKLSTPGQSPHGAYTTLLLHAFQATFGTHCD LPKLHRKLQSKTLEELEDIFTETTEAQELASGIGDVAEARQWLRTKLQAVGEKAGFPGILDTASPGKLHT IPIPVARCYTYSWNQDSFDILQEVLLKEQELLQPGILGDDEEEEEEDLEIDRHIAERDSLLSTGSLVSHD SELLLTSSQASGPVLSRQVLTTFVSGLSDGMDSGYVEDSEESSSEWPKKPGSQQRQGHRRPRQKFNRFYK LLKSTSQLVLRRDSRSLECSPDPTLPLRRAGSLSSPLDFPTQLPSRAQRSRSLPQPKLSTQLPSWLLAPP SHHQRRRPFLSGDEDPKASTLRVVVFGSDRISGKVARAYSKLRRLENSHPILTRFFKLQFFYVPVKRSHG ASSSACLSSLNQASPLLPDSLKHPSPADLGKAPWEDSTNDISHYLGMLDPWYERNVLGLMHLPPEVLCQQ SLKAESRPLEGSATQLPILADMLLYYCRFAARPVLLQVYQTELTFVTGEKTTEIFIQSLELGHSATTRAI KASGPGSKRLGIGDDREAVPLTLQIIYSKGAISGRSRWSNLEKVCTSVNLSKACQKPEELDSSMEALTLT LTEVVKRQNPKSKKGFNQISTSYIKVDKVQIISSSSCPFAVCLDQDERKILQSVVRCEVSPCYKPEKSSL PPEGSLSQPAEAGSDLCSLLCLPIMTFSGALP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191923
ORF Size	2616 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191923.1, NP_001178852.1
RefSeq Size	3031 bp
RefSeq ORF	2619 bp
Locus ID	497931
MW	96.7 kDa

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