Prmt9 (NM_001191599) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Prmt10; RGD1306157
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217254 representing NM_001191599 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGAACTCGAGGCCCAGGTCCCGCCGGAGCGCCGGGGGTGGCGCAGGTACGGCCGGCCGGGACCGGC TGGTGGCACAGTCCTTGCAGAGCGCTGAGCACTGTCTGGGCGACCAGGACTTCGGGACTGCCTACGCCCA TTACCTTCTGGTGCTCAGCTTGGCGCCAGAACTCAAAGAGGACGTGAAGGAAACTTTCCAGTATACACTT TTCAAGTGGGCTGAAGAGCTTCATGCACTGAATCGAATACAAGACTTACTTGGTTGCTACGAGCAGGCCT TAGAGCTGTTTCCTGATGATGAAGTGATTTGCAACAGTATGGGAGAGCATCTGTTCAGAATGGGCTTTCG AGATGAGGCAGCTGGGTATTTTCATAAAGCAGTGAAGCTAAACCCTGATTTCAACGATGCAAAGGAGAAC TTCTATCGTGTGGCAAACTGGTTGGTAGAGCGCTGGCACTTCATCATGCTTAATGACACCAAGAGAAATG AGATTTATAACGCGGCAATCCAGAAGGCAGTGCGGTTGGGCTCCAAAACAGTGCTGGACATTGGCGCCGG CACTGGGATACTGAGCATGTTTGCCAAAAAAGCTGGTGCACACTCAGTATACGCCTGTGAGTTATCCAAG ACCATGTATGAGCTCGCTTGTGACGTCGTGGCTGCAAACAAGATGGAGGATGGGATCAGGCTCCTGCACA TGAAGTCACTTGATCTAGAAATACCAAAGCACATTCCTGAAAGAGTGTCCCTAGTTGTAACAGAAACTGT CGATGCAGGTGTGTTTGGAGAAGGAATTGTGGAGAGTTTAATTCATGCATGGGAGCATTTACTTCTGCAA CCACAGACCAAAGGTGAAAGTGGAAGTTGGGGGAAGTATGGAAAAGTGATACCGGCCAGTGCGGTCATAT CTGGGATGGCAGTGGAGTGTGCAGAGATCCGAAGGCATCACAGGGTTAGTGCTAAGGACATAGCTGGTAT ACATTTACCAACAAATGTGAGATTTCAGAGCCCAGCCTATGCGTCTGTAGATCCCGAAGAAACGGTTGAA CCATACACAACGGAAAAGATGAGTGGAATCCCTGGAGGATACCGGCCTTTGACAGAATGCTTTCAGATTA TGAAAGTTGATTTCAATAATCTCCAGGAGTTAAAAAGTCTGGCAACTAGGAAGCCACACTCTCTTAGTGT ACCTGCTGTTAAGGAAGGCACGCTGGACGCGATTATGGTCTGGTTTGTTCTCCAGCTTGATGACGAGCAC AGTTTGTCCACAAGTCCCAGTGAGGAGACCTGTTGGGAACAGGCTGTTTATCCAGTACAGGCACTTGAAG ACTACTGGATACAGCCTGGAGACCAAGTGACAATGGAGGCATCTTGTCACGATTGTTACCTAAGAATCCA GGGGATCAGTATCTTGCATTTGGAACATGAAATGGAAGTTATAAAAAGTTTTACCAAGAGTAAAGACTTG CTCTCTCTGGGAAATGAGGCTGAGCTCTGTAGTGCCCTGGCTAACCTTCAGACCAGTAGACCAGAAGCTC TGGAGCAGACCTGTGTGTTAGAGCCCACAGAGATCGCTTTGCTTAATAACATTCCATATCATGAAGGCTT CAGGACAGCAATGAGGAAGGTATTGTCTTCACTGGCCCCAGAGACGCCGAGCCAGCCCATGGAAGCTCAC TGTCAGGACATGGAGACGAACTTGGAGAGTGCACCAAGCACCTCTGACCCTTTCTATGTGTTGGATGTGT CTGAAGGCTTCTCTCTTTTGCCTATACTTGCTGGAACCCTTGGTCAGGTTCAGCCCTACAGTTCTGTGGA GAAAGACCAGCACTGTATTGCTTTGGACCTCATATCTGAAGCCAATCACTTCCCTAAGGAAACACTTGAG TTTTGGCTGCGACACATAGAAGATGAAGCTGCTGTGTTGCAAAGACCCAAATCAGACAAGTTATGGAGTA TAATTATACTAGATGTCATTGAGCCGTCTGGGCTCATCCAGCAGGAGATCATGGAGAAGGCCGCAATATC CAGGTGTTTACTGCAGTCTGGAGGCAAGATTTTCCCGCAGTACGTGCTGATGTTTGGAATGCTGGTGGAG TCACAGACGCTGGTAGAAGAGAGTGCTGTTCAAGGAACAGAACACACTCTTGGGTTAAACATAGCACCGT TTATTAACCAGTTTCAGGTGCCTATCCGGGTCTGTTTGGATTTATCTTCCTTGCCATGCATTCCTTTAAG CCAGCCAGTGGAACTGTTGAGATTAGATCTGATGACTCCATATTTGAACACTTCCAATAGAGAAGTGAAG GTCCGTGTGTGCAGGTCTGGCCGATTGACCGCGGTTCCATTTTGGTTTCATTTGTGCCTGGATGACGAGG TCAGGTTGGACACCTCAGGTGAAGCGTCCCATTGGAAGCAGGCTGCAGTGGTCCTAGATAATCCCATCCA AGTACAAGCAGGAGAGGAACTTGTACTTAGTGTTGAGCATCACAAAAGCAACATCAGCATCACAGTGAAG CAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217254 representing NM_001191599 Red=Cloning site Green=Tags(s) MPNSRPRSRRSAGGGAGTAGRDRLVAQSLQSAEHCLGDQDFGTAYAHYLLVLSLAPELKEDVKETFQYTL FKWAEELHALNRIQDLLGCYEQALELFPDDEVICNSMGEHLFRMGFRDEAAGYFHKAVKLNPDFNDAKEN FYRVANWLVERWHFIMLNDTKRNEIYNAAIQKAVRLGSKTVLDIGAGTGILSMFAKKAGAHSVYACELSK TMYELACDVVAANKMEDGIRLLHMKSLDLEIPKHIPERVSLVVTETVDAGVFGEGIVESLIHAWEHLLLQ PQTKGESGSWGKYGKVIPASAVISGMAVECAEIRRHHRVSAKDIAGIHLPTNVRFQSPAYASVDPEETVE PYTTEKMSGIPGGYRPLTECFQIMKVDFNNLQELKSLATRKPHSLSVPAVKEGTLDAIMVWFVLQLDDEH SLSTSPSEETCWEQAVYPVQALEDYWIQPGDQVTMEASCHDCYLRIQGISILHLEHEMEVIKSFTKSKDL LSLGNEAELCSALANLQTSRPEALEQTCVLEPTEIALLNNIPYHEGFRTAMRKVLSSLAPETPSQPMEAH CQDMETNLESAPSTSDPFYVLDVSEGFSLLPILAGTLGQVQPYSSVEKDQHCIALDLISEANHFPKETLE FWLRHIEDEAAVLQRPKSDKLWSIIILDVIEPSGLIQQEIMEKAAISRCLLQSGGKIFPQYVLMFGMLVE SQTLVEESAVQGTEHTLGLNIAPFINQFQVPIRVCLDLSSLPCIPLSQPVELLRLDLMTPYLNTSNREVK VRVCRSGRLTAVPFWFHLCLDDEVRLDTSGEASHWKQAAVVLDNPIQVQAGEELVLSVEHHKSNISITVK Q TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191599
ORF Size	2523 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191599.1, NP_001178528.1
RefSeq Size	2742 bp
RefSeq ORF	2526 bp
Locus ID	291947
MW	93.9 kDa

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