Mapk7 (NM_001191547) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Bmk1; Erk5; Prkm7
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217226 representing NM_001191547 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGAACCGTTGAAGGAGGAGGACGGCGAAGATGGCTCTGGGGAACCCCCTGGGCGGGTGAAGGCAG AACCCGTCCACTCCGCTGCATCTGTCGTGGCCAAGAACCTGGCCCTGCTCAAAGCCCGCTCCTTCGACGT GACCTTTGACGTGGGGGACGAGTACGAGATCATCGAGACCATAGGCAATGGGGCCTACGGGGTGGTGTCC TCGGCGCGCCGCCGCCTCACGGGTCAGCAGGTGGCCATCAAGAAGATACCTAATGCTTTTGATGTGGTGA CCAATGCCAAACGGACCCTCAGGGAACTGAAGATCCTCAAACACTTCAAACACGACAATATCATCGCCAT CAAGGACATCCTGAGGCCCACTGTGCCCTATGGAGAATTCAGATCTGTCTACGTGGTACTGGACCTCATG GAAAGTGACCTACACCAGATCATTCACTCTTCACAGCCGCTCACACTAGAACATGTGAGATACTTCCTAT ACCAGCTGCTTCGGGGCCTCAAATACATGCACTCTGCTCAGGTCATCCACCGCGATCTTAAACCCTCTAA CCTTTTGGTCAATGAGAACTGTGAGCTCAAGATTGGTGACTTTGGAATGGCCCGTGGCCTGTGTACATCC CCTGCCGAGCACCAGTACTTCATGACTGAGTATGTGGCCACTCGCTGGTACCGTGCTCCTGAACTCATGC TTTCGCTGCATGAGTACACACAGGCTATTGACCTCTGGTCTGTGGGCTGCATCTTTGGTGAGATGCTGGC TCGGCGACAGCTCTTCCCAGGCAAAAACTATGTGCATCAGTTACAGCTGATCATGATGGTGTTGGGAACT CCATCACCAGCTGTGATTCAGGCTGTGGGGGCTGAAAGGGTGCGAGCCTATATCCAGAGCCTGCCACCAA GGCAGCCTGTACCTTGGGAGACAGTATACCCGGGTGCTGACCGCCAGGCCCTCTCACTGCTGGGACGCAT GTTGCGATTTGAACCCAGTGCCCGAATCTCAGCTGCTGCTGCCCTTCGCCACCCCTTCCTGGCTAAGTAC CACGACCCTGACGATGAGCCTGATTGCGCCCCACCTTTTGACTTTGCTTTTGACCGTGAAGCCCTTACCA GGGAGCGCATTAAGGAGGCCATTGTGGCTGAGATTGAGGACTTCCATGCACGACGGGAGGGCATCCGCCA ACAAATTCGCTTCCAGCCCTCTTTGCAGCCTGTGGCTAGTGAGCCTGTGTGTCCAGATGTCGAGATGCCC AGCCCCTGGGCTCCAAGTGGAGACTGTGCCATGGAGTCACCTCCTCCAGCACTACCACCATGCTCTGGCC CTGCCCCTGACACTGTTGATCTGACTCTGCAGCCTGCCCCGCCCGCCAGTGAGCTTGCTCCGCCAAAAAG AGAGGGTGCCATCTCCGACAATACCAAAGCAGCCCTCAAAGCTGCTCTGCTCAAGTCCCTGAGGAGCCGG CTTCGAGATGGGCCCAGTGCACCCTTGGAGGCCCCTGAACCTCGAAAGCCTGTGACAGCTCAGGAACGTC AGCGAGAGCGAGAAGAGAAGCGCAGGAGGCGACAAGAGAGAGCCAAGGAGAGGGAGAAGCGACGACAAGA GCGGGAGCGCAAGGAGAGGGGGGCTGGCACCTTGGGGGGCCCCTCTACTGACCCTTTGGCTGGATTGGTG CTCAGTGACAATGACCGAAGCCTGCTAGAGCGGTGGACTCGCATGGCTAGGCCTCCTGTCCCTGCACCAG CACCAGCACCAGCACCAACACCAAAACCAACACCAACACCAACACAGTCCTCTGCTCAACCCACTAGTCC TCCCAATGGCCCTGTATCTCAGTCTACTGCTCCTCTACAGCCTGCAGGCTCTATTCCTGGTCCTGCCTCC CAGCCTGTTTGCCCACCCCCTGGGCCTGTTCCCCAGCCTGCTGGCCCTGTCCCTGCCCCTCTCCAGACTG CCCCTTCCACTAGCCTTTTGGCCTCCCAGTCACTTGTGCCACCTAGTGGGTTGCCTGGTTCTGGTGCCCC AGAAGTTCTGCCTTACTTCCCATCTGGCCCACCACCTCCAGATCCTGGGCTCACCCCTCAGCCTTCTACA TCAGAGTCACCTGATGTCAACCTGGTGACCCAGCAACTGTCCAAGTCTCAGGTGGAGGACCCCCTGCCTC CCGTGTTCTCAGGCACTCCAAAGGGCAGTGGGGCTGGCTACGGTGTTGGCTTTGACCTGGAGGAATTCTT AAACCAATCTTTTGACATGGGTGTGGCTGATGGGCCACAGGATAGCCAGGCAGACTCAGCCTCACTCTCA GCCTCCCTCCTTGCTGACTGGCTTGAGGGCCACGGCATGAACCCCGCTGACATTGAGTCTCTGCAGCGTG AGATCCAGATGGACTCCCCAATGTTGCTGTCTGACCTGCCTGACCTCCAAGAACCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217226 representing NM_001191547 Red=Cloning site Green=Tags(s) MAEPLKEEDGEDGSGEPPGRVKAEPVHSAASVVAKNLALLKARSFDVTFDVGDEYEIIETIGNGAYGVVS SARRRLTGQQVAIKKIPNAFDVVTNAKRTLRELKILKHFKHDNIIAIKDILRPTVPYGEFRSVYVVLDLM ESDLHQIIHSSQPLTLEHVRYFLYQLLRGLKYMHSAQVIHRDLKPSNLLVNENCELKIGDFGMARGLCTS PAEHQYFMTEYVATRWYRAPELMLSLHEYTQAIDLWSVGCIFGEMLARRQLFPGKNYVHQLQLIMMVLGT PSPAVIQAVGAERVRAYIQSLPPRQPVPWETVYPGADRQALSLLGRMLRFEPSARISAAAALRHPFLAKY HDPDDEPDCAPPFDFAFDREALTRERIKEAIVAEIEDFHARREGIRQQIRFQPSLQPVASEPVCPDVEMP SPWAPSGDCAMESPPPALPPCSGPAPDTVDLTLQPAPPASELAPPKREGAISDNTKAALKAALLKSLRSR LRDGPSAPLEAPEPRKPVTAQERQREREEKRRRRQERAKEREKRRQERERKERGAGTLGGPSTDPLAGLV LSDNDRSLLERWTRMARPPVPAPAPAPAPTPKPTPTPTQSSAQPTSPPNGPVSQSTAPLQPAGSIPGPAS QPVCPPPGPVPQPAGPVPAPLQTAPSTSLLASQSLVPPSGLPGSGAPEVLPYFPSGPPPPDPGLTPQPST SESPDVNLVTQQLSKSQVEDPLPPVFSGTPKGSGAGYGVGFDLEEFLNQSFDMGVADGPQDSQADSASLS ASLLADWLEGHGMNPADIESLQREIQMDSPMLLSDLPDLQEP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191547
ORF Size	2436 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191547.1, NP_001178476.1
RefSeq Size	2738 bp
RefSeq ORF	2439 bp
Locus ID	114509
MW	88.5 kDa
Gene Summary	binds and phosphorylates myocyte enhancer factor 2 (Mef2); may regulate Mef2 transcription activator function [RGD, Feb 2006]

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