Tle1 (NM_001173433) Rat Tagged ORF Clone
CAT#: RR217192
- TrueORF®
Tle1 (myc-DDK-tagged) - Rat transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila) (Tle1)
"NM_001173433" in other vectors (1)
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CNY 7,320.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR217192 representing NM_001173433
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCCTCAGAGCCGGCACCCAACGCCGCACCAGGCTGCAGGCCAGCCCTTTAAGTTCACTATCCCGG AGTCCCTGGACCGGATTAAAGAGGAATTCCAGTTCCTGCAGGCGCAGTATCACAGTCTTAAATTGGAGTG TGAGAAATTGGCAAGTGAAAAGACAGAAATGCAGAGACACTACGTGATGTATTATGAAATGTCATATGGA TTAAACATTGAAATGCATAAACAGACCGAAATCGCCAAGAGATTGAATACCATTTGTGCACAAGTCATCC CATTTCTGTCTCAGGAACATCAACAACAGGTGGCCCAGGCTGTCGAACGTGCCAAACAGGTGACCATGGC AGAGTTGAATGCCATCATCGGGCAGCAGCAGTTGCAAGCTCAGCATCTCTCCCATGGCCATGGACCCCCA GTACCTCTCACGCCTCACCCTTCAGGACTTCAGCCTCCTGGCATCCCGCCCCTTGGGGGCAGTGCCAGCC TTCTCGCGCTGTCTAGTGCTCTGAGTGGGCAGTCTCACTTGGCAATAAAAGATGACAAGAAGCACCATGA TGCAGAGCGCCACAGAGACAGAGAGCCGGGCACGAGTAATTCCCTCTTGGTCCCAGACAGCCTAAGAGGC ACAGATAAACGCAGAAATGGTCCAGAGTTTTCCAGTGACATCAAAAAACGGAAGGTGGATGATAAGGATA ACTATGACAGTGATGGGGACAAGAGTGACGACAACTTAGTTGTGGATGTGTCTAATGAGGACCCTTCTTC TCCACATGCAAGCCCCACACACTCACCCCGGGAAAACGGAATTGACAAAAACCGTCTGTTGAAGAAAGAT GCTTCAGGTAGCCCGGCATCCACAGCCTCCTCTGGAAGTTCCTCATCCCTGAAATCCAAAGAAGTGAACC TGCATGAAAAAGCCAACACGCCTGTTCTGAAATCCAGCACACCAACGCCTCGGAGTGACATGCCAACCCC GGGCACCAGCGCTACTCCAGGCCTCCGTCCAGGTCTCGGCAAGCCTCCAGCCATGGAGCCCCTTGTCAAC CAAGCAGCAGCTGGCCTGAGGACGCCCCTGGCAGTGCCTGGCCCATACCCTGCCCCCTTTGGCATGGTGC CCCATGCAGGCATGAACGGAGAGCTGACCAGCCCTGGCGCTGCCTATGCAGGTCTACACAGCATGTCTCC ACAGATGAGCGCTGCAGCTGCTGCAGCTGCTGCTGCTGTGGTGGCCTATGGGCGCTCCCCAATGGTTGGT TTTGATCCTCCTCCTCACATGAGAGTACCTTCTATCCCTCCCAACCTGGCAGGAATACCTGGCGGGAAAC CAGCATACTCCTTCCACGTTACGGCTGACGGACAAATGCAGCCTGTCCCTTTTCCCCCTGACGCCCTCAT TGGACCTGGGATTCCCCGACATGCTCGGCAGATCAATACCCTCAACCACGGGGAGGTGGTGTGTGCAGTG ACCATCAGCAACCCCACAAGGCACGTGTACACAGGTGGCAAGGGCTGCGTTAAGGTCTGGGACATCAGCC ACCCTGGCAACAAGAGCCCCGTCTCTCAGCTGGATTGTCTGAACAGAGATAACTACATCCGTTCCTGTAA ATTGCTACCTGATGGCTGTACTCTCATAGTGGGAGGGGAAGCCAGTACTCTATCCATCTGGGACCTGGCA GCTCCAACTCCCCGAATTAAGGCAGAGCTGACATCCTCAGCCCCTGCCTGCTATGCTCTGGCCATCAGCC CTGACTCCAAGGTCTGCTTCTCATGCTGCAGTGATGGCAACATCGCAGTATGGGACCTGCACAACCAGAC CCTGGTGAGGCAATTCCAGGGCCACACAGACGGAGCCAGCTGTATTGACATTTCTAATGATGGCACCAAG CTCTGGACAGGCGGTTTAGACAACACTGTGAGGTCCTGGGACCTGAGAGAAGGAAGGCAGCTGCAGCAGC ATGATTTCACTTCACAGATCTTCTCCTTGGGATACTGCCCAACTGGGGAGTGGCTTGCCGTGGGCATGGA AAGCAGCAACGTGGAAGTTCTGCATGTGAACAAGCCTGACAAATACCAGCTTCACCTCCACGAGAGTTGT GTGCTGTCCCTAAAATTTGCTTATTGTGGCAAGTGGTTTGTGAGTACTGGAAAAGACAACCTCCTAAATG CCTGGCGGACACCATATGGAGCCAGCATATTCCAGTCCAAAGAATCTTCATCAGTGCTTAGCTGTGACAT CTCTGTGGATGATAAATACATAGTCACTGGCTCAGGGGACAAAAAGGCTACCGTTTATGAGGTCATCTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR217192 representing NM_001173433
Red=Cloning site Green=Tags(s) MFPQSRHPTPHQAAGQPFKFTIPESLDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSYG LNIEMHKQTEIAKRLNTICAQVIPFLSQEHQQQVAQAVERAKQVTMAELNAIIGQQQLQAQHLSHGHGPP VPLTPHPSGLQPPGIPPLGGSASLLALSSALSGQSHLAIKDDKKHHDAERHRDREPGTSNSLLVPDSLRG TDKRRNGPEFSSDIKKRKVDDKDNYDSDGDKSDDNLVVDVSNEDPSSPHASPTHSPRENGIDKNRLLKKD ASGSPASTASSGSSSSLKSKEVNLHEKANTPVLKSSTPTPRSDMPTPGTSATPGLRPGLGKPPAMEPLVN QAAAGLRTPLAVPGPYPAPFGMVPHAGMNGELTSPGAAYAGLHSMSPQMSAAAAAAAAAVVAYGRSPMVG FDPPPHMRVPSIPPNLAGIPGGKPAYSFHVTADGQMQPVPFPPDALIGPGIPRHARQINTLNHGEVVCAV TISNPTRHVYTGGKGCVKVWDISHPGNKSPVSQLDCLNRDNYIRSCKLLPDGCTLIVGGEASTLSIWDLA APTPRIKAELTSSAPACYALAISPDSKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDISNDGTK LWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHVNKPDKYQLHLHESC VLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISVDDKYIVTGSGDKKATVYEVIY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001173433 |
ORF Size | 2310 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001173433.1, NP_001166904.1 |
RefSeq Size | 2892 bp |
RefSeq ORF | 2313 bp |
Locus ID | 362533 |
MW | 83.1 kDa |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN217192 | Tle1 (untagged) - Rat transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila) (Tle1) |
CNY 7,320.00 |