Rps6ka2 (NM_057128) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217149 representing NM_057128 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTGAACATGAAGAAGTTCACGGTGCGCAGGTTCTTCTCAGTGTACCTGCGCAAGAAGTCGCGCT CCAAGAGCTCCAGTCTGAGTCGCCTCGAGGAAGAAGGCATCGTGAAGGAGATTGACATTAGCAGCCATGT GAAGGAAGGCTTCGAGAAGGCAGACCCCTCCCAGTTCGAGCTGCTAAAAGTTTTAGGACAAGGGTCGTAT GGAAAGGTGTTCTTGGTGAGGAAAGTCACAGGATCGGATGCTGGTCAGCTCTACGCCATGAAAGTCCTGA AGAAAGCCACCTTGAAAGTGCGGGACCGGGTCCGGTCTAAGATGGAGAGAGATATCCTGGCAGAGGTGAA TCACCCTTTCATCGTCAAGCTGCATTACGCCTTTCAGACCGAAGGCAAGCTCTACCTGATCCTGGACTTC CTGCGGGGAGGAGACCTCTTCACCAGGCTTTCCAAAGAGGTGATGTTTACGGAGGAGGATGTTAAGTTCT ACCTGGCTGAGCTGGCCTTGGCTCTGGACCACCTCCACGGCCTAGGGATCATCTACAGGGATCTGAAGCC TGAAAATATCCTCCTGGATGAAGAGGGACATATTAAGATCACAGATTTTGGCTTGAGCAAGGAGGCCATC GACCATGACAAGAGAGCCTATTCATTTTGTGGGACTATTGAATACATGGCGCCCGAGGTGGTGAACCGGC GTGGACACACACAGAGTGCTGACTGGTGGTCCTTTGGTGTGCTCATGTTCGAGATGCTCACAGGATCCCT GCCGTTTCAGGGGAAGGACAGGAAGGAAACAATGGCCCTCATCCTCAAAGCCAAGCTGGGCATGCCTCAG TTCCTCAGTGCGGAGGCACAGAGCCTGCTGAGAGCCCTTTTCAAGCGGAATCCCTGTAACCGGCTAGGTG CTGGTGTTGACGGAGTGGAGGAAATTAAACGTCACCCGTTCTTTGTCACCATAGACTGGAATAAGCTGTA TCGCAAGGAAATCAAGCCACCCTTCAAGCCAGCAGTGGGAAGGCCTGAGGACACCTTCCACTTTGACCCC GAGTTTACTGCAAGGACCCCCACAGACTCTCCTGGTGTCCCCCCAAGTGCAAACGCCCACCACCTGTTCA GGGGGTTCAGCTTTGTGGCCTCCAGCCTGGTCCAGGAGCCTTCACAGCAAGACGTGCCCAAGGCCCCCAT TCACCCGATTGTGCAGCAGTTACATGGGAACAATATCCACTTCACTGATGGCTACGAGATCAAGGAGGAC ATCGGGGTGGGCTCCTACTCAGTGTGCAAGCGGTGTGTACACAAAGCCACGGATGCCGAGTACGCCGTGA AGATCATCGATAAGAGCAAAAGGGATCCCTCAGAGGAGATTGAGATCCTCCTGCGGTATGGCCAACACCC CAACATCATCACCCTAAAAGATGTCTATGATGATGGAAAATACGTATACCTGGTGATGGAGCTCATGCGA GGTGGGGAGCTGCTGGACCGCATCCTCCGCCAGCGGTGCTTCTCAGAGCGGGAGGCCAGTGATGTGCTGT ACACCATCGCCAGGACCATGGACTACCTGCACTCCCAAGGGGTTGTTCATCGGGACCTGAAACCAAGTAA CATTCTGTACATGGATGAATCTGGAAACCCCGAATCTATCCGCATCTGTGACTTTGGGTTTGCCAAACAG CTGCGAGCAGAAAATGGACTGCTCATGACCCCCTGCTATACTGCGAACTTCGTGGCTCCTGAGGTCCTGA AGCGGCAAGGCTACGACGCAGCGTGTGATGTCTGGAGCCTGGGAATCCTGCTGTACACCATGCTGGCTGG GTTCACCCCTTTTGCTAATGGGCCAGACGATACCCCCGAGGAGATTCTGGCGAGGATTGGCAGTGGGAAG TACGCCCTTTCTGGAGGAAACTGGGACTCCATATCGGATGCAGCAAAGGACGTCGTGTCCAAGATGCTCC ACGTGGACCCGCAGCAACGCCTAACAGCAGTTCAAGTGTTGAAGCACCCATGGATCGTGAACAGAGAGTA CCTATCCCAAAACCAGCTGAGCAGACAGGACGTCCATCTAGTGAAGGGTGCCATGGCAGCCACCTACTTT GCTCTGAACAGGACCCCACAGGCGCCGAGGCTGGAGCCTGTGCTCTCCTCTAGCTTGGCCCAACGCAGAG GCATGAAGAGACTCACGTCTACCCGGTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217149 representing NM_057128 Red=Cloning site Green=Tags(s) MELNMKKFTVRRFFSVYLRKKSRSKSSSLSRLEEEGIVKEIDISSHVKEGFEKADPSQFELLKVLGQGSY GKVFLVRKVTGSDAGQLYAMKVLKKATLKVRDRVRSKMERDILAEVNHPFIVKLHYAFQTEGKLYLILDF LRGGDLFTRLSKEVMFTEEDVKFYLAELALALDHLHGLGIIYRDLKPENILLDEEGHIKITDFGLSKEAI DHDKRAYSFCGTIEYMAPEVVNRRGHTQSADWWSFGVLMFEMLTGSLPFQGKDRKETMALILKAKLGMPQ FLSAEAQSLLRALFKRNPCNRLGAGVDGVEEIKRHPFFVTIDWNKLYRKEIKPPFKPAVGRPEDTFHFDP EFTARTPTDSPGVPPSANAHHLFRGFSFVASSLVQEPSQQDVPKAPIHPIVQQLHGNNIHFTDGYEIKED IGVGSYSVCKRCVHKATDAEYAVKIIDKSKRDPSEEIEILLRYGQHPNIITLKDVYDDGKYVYLVMELMR GGELLDRILRQRCFSEREASDVLYTIARTMDYLHSQGVVHRDLKPSNILYMDESGNPESIRICDFGFAKQ LRAENGLLMTPCYTANFVAPEVLKRQGYDAACDVWSLGILLYTMLAGFTPFANGPDDTPEEILARIGSGK YALSGGNWDSISDAAKDVVSKMLHVDPQQRLTAVQVLKHPWIVNREYLSQNQLSRQDVHLVKGAMAATYF ALNRTPQAPRLEPVLSSSLAQRRGMKRLTSTRL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_057128
ORF Size	2199 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_057128.1, NP_476469.1
RefSeq Size	5396 bp
RefSeq ORF	2202 bp
Locus ID	117269
MW	83.2 kDa
Gene Summary	ribosomal protein; may be upregulated by the insulin signaling pathway in skeletal muscle [RGD, Feb 2006]

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