Rbm14 (NM_133388) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CoAA
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217081 representing NM_133388 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGATCTTTGTAGGCAATGTCGACGGGGCGGATACGACGCCGGAGGAGCTGGCAGCCCTCTTCGCGC CCTATGGCACGGTCATGAGCTGCGCCGTCATGAAACAGTTTGCCTTCGTGCACATGCGCGAGAACGCTGG CGCGGTGCGCGCCATCGAGGCCCTGCATGGCCACGAGCTGCGTCCAGGTCGCGCGCTCGTGGTGGAGATG TCGCGCCCGAGGCCCCTGAACACTTGGAAGATTTTCGTGGGCAATGTATCGGCTGCATGCACAAGCCAGG AATTGCGCAGCCTGTTCGAGCGCCGTGGACGCGTCATCGAGTGTGACGTGGTAAAAGACTACGCGTTTGT TCACATGGAGAAGGAAGCAGATGCCAAAGCCGCCATCGCGCAGCTCAACGGCAAAGAAGTGAAGGGCAAG CGCATCAACGTGGAACTCTCAACCAAGGGGCAGAAGAAGGGGCCTGCCCTGGCTATCCAGTCTGGGGACA AGACCAAGAAACCAGGGGCTGGGGATACAGCATTCCCTGGAACTGGTGGCTTCTCTGCCACCTTCGACTA CCAGCAGGCTTTTGGCAACAGCACTGGTGGCTTTGATGGGCAAGCCCGTCAGCCCACACCACCATTCTTT GGTCGCGACCGCAGCCCCCTGCGCCGTTCACCTCCTCGAGCCTCTTATGTGGCTCCTCTGACGGCCCAGC CAGCCACCTACCGGGCCCAACCCTCTGTGTCTCTGGGAGCTGCATATAGGGCCCAGCCTTCGGCCTCTTT GGGTGTCGGCTATCGGACTCAGCCCATGACAGCCCAGGCAGCTTCGTACCGTGCTCAACCCTCTGTCTCC CTTGGGGCCCCATACAGGGGTCAACTGGCTAGCCCTAGTTCCCAGTCTGCTGCAGCTTCCTCACTTGGCC CATATGGTGGAGTCCAGCCCTCAGCCTCAGCCCTTTCCACCTATGGGGGTCAGGCAGCTGCAGCTTCTTC GCTTAATTCCTATGGGGCTCAGGGCTCCTCCCTTGCTTCCTATGGTAACCAGCCATCCTCTTATGGCGCC CAGGCTGCCTCTTCATATGGGGTTCGTGCGGCTGCTTCTTCCTATAACACACAGGGAGCAGCTTCTTCCC TAGGTTCCTATGGGGCTCAAGCAGCCTCCTATGGTGCCCAGTCTGCAGCCTCCTCACTAGCTTATGGAGC CCAGGCAGCCTCTTACAATGCCCAGCCCTCAGCCTCTTACAGTGCCCAGTCTGCCCCATATGCTGCACAA CAGGCTGCTTCTTATTCTTCCCAGCCTGCTGCTTATGTGGCACAGCCAGCCGCAGCTGCTGCCTATGCTA GCCAGCCAGCTGCCTATGCTGCACAGGCCACTACCCCAATGGCCGGTTCCTATGGGGCTCAACCAGTTGT TCAGACCCAGCTGAATAGTTATGGAGCTCAAGCATCTATGGGCCTGTCAGGCTCATATGGAGCTCAGTCT GCTGCTGCGGCCACTGGCTCCTATGGTGCAGCAGCTGCCTATGGGGCGCAGCCTTCTGCCACCTTGGCAG CTCCTTACCGCACTCAGTCATCAGCCTCATTGGCTGCTTCCTATGCTGCCCAGCAGCATCCCCAGGCTGC TGCCTCCTACCGTGGCCAGCCGGGCAGTGCCTACGATGGGACAGGCCAGCCGTCAGCAGCCTACCTGTCT ATGTCCCAGGGGGCCGTTGCCAACGCCAACAGCACCCCACCGCCCTATGAGCGTACCCGCCTCTCCCCAC CCCGGGCCAGCTACGACGATCCCTACAAAAAGGCTGTCGCCATGTCGAAAAGGTATGGTTCCGACCGGCG TTTAGCCGAGCTCTCTGATTACCGCCGTTTATCAGAGTCGCAGCTTTCGTTCCGCCGCTCGCCGACAAAG TCCTCGCTGGATTACCGTCGCCTGCCCGATGCCCATTCCGATTACGCACGCTATTCGGGCTCCTATAATG ATTACCTGCGGGCAGCTCAGATGCACTCTGGCTACCAGCGCCGCATG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217081 representing NM_133388 Red=Cloning site Green=Tags(s) MKIFVGNVDGADTTPEELAALFAPYGTVMSCAVMKQFAFVHMRENAGAVRAIEALHGHELRPGRALVVEM SRPRPLNTWKIFVGNVSAACTSQELRSLFERRGRVIECDVVKDYAFVHMEKEADAKAAIAQLNGKEVKGK RINVELSTKGQKKGPALAIQSGDKTKKPGAGDTAFPGTGGFSATFDYQQAFGNSTGGFDGQARQPTPPFF GRDRSPLRRSPPRASYVAPLTAQPATYRAQPSVSLGAAYRAQPSASLGVGYRTQPMTAQAASYRAQPSVS LGAPYRGQLASPSSQSAAASSLGPYGGVQPSASALSTYGGQAAAASSLNSYGAQGSSLASYGNQPSSYGA QAASSYGVRAAASSYNTQGAASSLGSYGAQAASYGAQSAASSLAYGAQAASYNAQPSASYSAQSAPYAAQ QAASYSSQPAAYVAQPAAAAAYASQPAAYAAQATTPMAGSYGAQPVVQTQLNSYGAQASMGLSGSYGAQS AAAATGSYGAAAAYGAQPSATLAAPYRTQSSASLAASYAAQQHPQAAASYRGQPGSAYDGTGQPSAAYLS MSQGAVANANSTPPPYERTRLSPPRASYDDPYKKAVAMSKRYGSDRRLAELSDYRRLSESQLSFRRSPTK SSLDYRRLPDAHSDYARYSGSYNDYLRAAQMHSGYQRRM SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_133388
ORF Size	2007 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_133388.1, NP_596879.1
RefSeq Size	3144 bp
RefSeq ORF	2010 bp
Locus ID	170900
MW	69.5 kDa
Gene Summary	human homolog interacts with thyroid hormone receptor binding protein; plays a role in transcriptional coactivation [RGD, Feb 2006]

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