Map7 (NM_001198638) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Mtap7
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217043 representing NM_001198638 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGCAGGGAGCTGGAGGCGACGGCCACAGGGGTGGCGACGGCGCTACGCAGAGCGACCCAGCGT CTGATGGCTATAAAATACAAGGAAAGAGACCTGCCCCCAGCCGCCCTACTTCCACACTTTCAGGACAAAC GAGCAACCACTCAGGAAATAAACCAGATCCCCCACCTGTGCTACGGGTTGATGACCGGCAGCGTCTGGCC AGAGAACGCCGTGAGGAACGAGAAAAACAGCTAGCTGCACGAGAAACCGTCTGGCTAGAGAGAGAAGAGC GAGCCCGGCAGCACTATGAGAGGCACCTGGAGGCACGGAAGAAGAAGCTGGAGGACCAGCGGCTGAAGGA GGAGCGGAGGAGAGCTGCGGTGGAGGAGAAGCGGAGACAGAGGCTGGAGGAGGACAAGGAGCGCCATGAA GCTGTTGTTCGGCGGACGATGGAAAGGAGCCAGAAGCCGAAGCAGAAGTATAACCGGTGGTCGTGGGGAA GCCCTCTCCACGGGAGCTCTAGCATTCACAGTGGAGCATCTTGCAGCCCCATCACCACGCCCTTCAAAAC TGCACACTCTAGAAACCCAGTGGATCGACCAAAACTCTTTATAACGCCGCCCGAGGGCTCTGCTCGGAGG AGGACCATTCACGGGATATCGAGCTATAAAAGAGAACGAGAACACATTCCCTTCCACGTTTCCCCTGGTG TCCGAAGGACTCTGTCTCCATCTAATCTCAAGGCGAGATCCCCAGCTCCAGCCCGCCTTTGGCCCCCATC CAAGTCCATGCCTCATCTGCCCGGGACACCCCGACCGGCATCCTCCTTGACTCCTGGCCCCGTCAGAGCT GCTGCTTCTTCCTCGTCTCCTGGCAACATCCGCCCTGTCAAGAGAGAAGTGAAAGTGGAGCCTGAGAAAA AAGACCCCGAGAAGAAAGTGACCAATGACCCCACAATAAAGGGCAGGGTGCCATTAGTGAAGGTGGAGGA GGGCACAGTTGAAGAGGGGACACCCGTGAAGCCACCTGACCCTGCTGTTCCAGCCCCCACTCCAGCGACG GACCCAGCCCCAGCACCACCATCACCATCCTCTGTGAGTGTCGGTGTAATTCCTAAGACTTCTGCAGGCA CCACTGACCCAGAAGAGGCTACGAGGCTGCTGGCTGAGAAGAGGCGTCTAGCCAGAGAGCAAAGGGAGAA GGAAGAAAGGGAGAGGAAGGAGAAGGAAGAGCTGGAGAGACAAAAGATAGAGGAATTGGCGCGAAGGGTG GCTGAAGAGCGAAGTCGCAGGGAAGAGGAAGCGCGCAGGCTGGAAGCTGAGCAGGCGCGCAAGAGAGAGG AGCTGGCGCTGCGTCTGGCTGAAGAGGAGCGGGAGCGGTGGGAGAGGGAGGAGGTGGAGCGCGCACAGAA GCAGAAAGAAGAAGAAGCCCGAGCCCGGGAGGAGGCAGAGAAGGCTCGGCAGGAACGAGAGAAACATTTT CAGAAAGAAGAGCAGGAGCGCCTGGAGAGGAAGAAGCGACTTGAAGAGATCATGAGAAGAACCAGAAGGA CAGAGACAGCGGATAAGAAAACCACTGGGCAAAGCAATGGCGACATGGTCAAAGGAGTTCTCGCCGGAGA GACAGAGGTATCTGCCCTGCCAGGTGTGAACTCTCCAGGACATGTCGAGTCTGCAGGCAGCACACATGGA GTGGCTTTGCAGCAATCAAAAGTGACCATAGAGAGTTCTCCAGATTTGGAGAAGCAGCCAAATGAAAATG GAATGTCCACACAAAATGAAAATTTTGAAGAAATTATAAACTTACCTGTTGGATCAAAAGCATCCAGATT AGACGTCTCCAATGAGAACCCAGAAATTCCCTTGAAACCAATTTTGGCCTTTAATGATGAAGGGACACTT GGGCCCCTACCTCAGGTGGATGGTGTGCAGACACAACAGACCACAGAAGTTATA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217043 representing NM_001198638 Red=Cloning site Green=Tags(s) MAEQGAGGDGHRGGDGATQSDPASDGYKIQGKRPAPSRPTSTLSGQTSNHSGNKPDPPPVLRVDDRQRLA RERREEREKQLAARETVWLEREERARQHYERHLEARKKKLEDQRLKEERRRAAVEEKRRQRLEEDKERHE AVVRRTMERSQKPKQKYNRWSWGSPLHGSSSIHSGASCSPITTPFKTAHSRNPVDRPKLFITPPEGSARR RTIHGISSYKREREHIPFHVSPGVRRTLSPSNLKARSPAPARLWPPSKSMPHLPGTPRPASSLTPGPVRA AASSSSPGNIRPVKREVKVEPEKKDPEKKVTNDPTIKGRVPLVKVEEGTVEEGTPVKPPDPAVPAPTPAT DPAPAPPSPSSVSVGVIPKTSAGTTDPEEATRLLAEKRRLAREQREKEERERKEKEELERQKIEELARRV AEERSRREEEARRLEAEQARKREELALRLAEEERERWEREEVERAQKQKEEEARAREEAEKARQEREKHF QKEEQERLERKKRLEEIMRRTRRTETADKKTTGQSNGDMVKGVLAGETEVSALPGVNSPGHVESAGSTHG VALQQSKVTIESSPDLEKQPNENGMSTQNENFEEIINLPVGSKASRLDVSNENPEIPLKPILAFNDEGTL GPLPQVDGVQTQQTTEVI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001198638
ORF Size	1944 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001198638.1, NP_001185567.1
RefSeq Size	3564 bp
RefSeq ORF	1947 bp
Locus ID	293016
MW	73.1 kDa

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