Rab11fip1 (NM_001197241) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Rab11-FIP1; RGD1562965
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217041 representing NM_001197241 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCTGGCGGCCTCAGCGGGCCGGGGCCCGGGGACTATGTGGTCCCCTACGCACGTACAGGTGACGG TGCTACAGGCACGAGGCCTGCGGGCCAAGGGTCCCGGGGGCACGAGCGACGCGTACGCGGTGATCCAGGT GGGCAAGGAGAAGTACGCCACGTCGGTGTCCGAGCGCAGCCTGGGCGCGCCCGTGTGGCGCGAGGAAGCC ACCTTCGAGCTGCCGCCGCTCCTGTCCTCCGGGACCGCGCCCGCCGCCGCCGCCGCCACCCTGCAACTCA CCGTCCTGCACCGCGCGCTGCTCGGTCTCGATAAGTTCCTGGGTCGCGCCGAAGTGGACCTGCGGGAGCT GCACCGGGACCAGAGCCGCAGGAAGAAGCAATGGTACACCTTGAAGTCCAAACCCGGAAAGAAGGATAAA GAGCGAGGAGAAATCGAGGTGGACATCCAGTTTATGAGAAACAACATGACGGCCAGCATGTTTGACCTCT CCATGAAGGATAAGTCTCGCAATCCGTTTGGGAAACTGAAGGACAAGATCAAAGGGAAGAATAAGGACAA CACCTCGGACACCGCCTCGGCCATCGTCCCCAGCACGACACCCTCGGTCGACAGCGATGACGAGTCCTTC TCAAAAGACAAGAAGAAGAAGTCAAAGATCAAGACTTTGTTTTCCAAGCCGAGTTTGCAGAAGACCCCGC TCTCCCAGTCCATGTCTGTCCTGCCCACTTCCAAGCCAGACAAAGTCCTGCTGCGGCCAGGAGACTTCCA GTCCCGGTGGGGTGACGAGGGCGACAATGAGGACGAGTCCTCCTCTGCCTCGGAGGTCATGTCCCAAAAG CGAACATCCAGTACAGACCATACACAACCGAACCAGTCCAACTTCAGCCTTCCCAAGAAAGAAGGGCTCT CTTTCCTCGGTGGCCTCCGGTCCAAGAATGACTCCCTTTCCCGGTCTAACGTCTGTATCAACGGGAACCA CGTTTACATGGAGCAGCCAGAGGCCAAGAGTGAGATCAGGGAGAGCAGCCCTTCTAACTCCCCCTCCCCG CAGGGCTTCAGGAAAAGGCATTTGTTCTCGTCCACTGAAAACCTGGCTGCCCGGTCTCCTAAGGAGCCAG GGGAAGGAGGTGGCATGTCCTCTGACAGGCGACTGTCCGACTCCTCCACCAAGGACTCCATGAAATCCAT GTCTTTGCCATCCTACCGACCCCTGACCACTGCGGACAGTAGAGAGGGCTTATCTCCAGCAAACATGGAG GTGGCTACCAAAGAGACAAAAGACAGCAAGAAGCAGGAGAGCAAGAAGTCCTCTTTGCTTTCTCTGGTAA CAGGAAAGAAAGATGTGGCTAAAGGCAGTGAAGGCGAACCTCTTCCCCCTGTCTCAGAGAAAGAGAAGGG AAGGAAGGGTGTGCTCGTGGAAGCCCAGCTTCGGGAGGAAGATCTGGTGAGAAGACCTGAGAAAGATGCT GTACCTGTTGCCTCCCAATGGGGCAGCTCCCAGAACCCCTTTGAGGACGCACAGATCTCAGACCTGGAAG CCAGTGTGGAGTCTAAGTGTGAACCGAAACCACCAGTTCCTGTTCCAAGGACTCCCCAGACCAGAGCTGT GAAGCCTAGGCCTCACCCCGTGAAGCCGATGAACACAACAGCCCCCAAGATAACAAACTCTAGCTTGGGA ACCGCCACCATCATCAGTGAGAACCTGATCAATGAGGCCCTCATGAAGAAATACCAGCCCTCGGATCCAG CCTTTGCCTATGCCCAGCTGACCCACGATGAGCTGATCCAGTTGGTCCTCAAACAGAAGGAAACCATCAG CAAGAAGGAGTTCCAGGTGCGGGAGCTGGAAGATTACATCGACAACCTGCTGGTCAGAGTCATGGAGGAG ACCCCCAACATCCTCCGAGTGCCAGCGCAGACAGGCAAAAAGGCTGGAAAGATG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217041 representing NM_001197241 Red=Cloning site Green=Tags(s) MSLAASAGRGPGTMWSPTHVQVTVLQARGLRAKGPGGTSDAYAVIQVGKEKYATSVSERSLGAPVWREEA TFELPPLLSSGTAPAAAAATLQLTVLHRALLGLDKFLGRAEVDLRELHRDQSRRKKQWYTLKSKPGKKDK ERGEIEVDIQFMRNNMTASMFDLSMKDKSRNPFGKLKDKIKGKNKDNTSDTASAIVPSTTPSVDSDDESF SKDKKKKSKIKTLFSKPSLQKTPLSQSMSVLPTSKPDKVLLRPGDFQSRWGDEGDNEDESSSASEVMSQK RTSSTDHTQPNQSNFSLPKKEGLSFLGGLRSKNDSLSRSNVCINGNHVYMEQPEAKSEIRESSPSNSPSP QGFRKRHLFSSTENLAARSPKEPGEGGGMSSDRRLSDSSTKDSMKSMSLPSYRPLTTADSREGLSPANME VATKETKDSKKQESKKSSLLSLVTGKKDVAKGSEGEPLPPVSEKEKGRKGVLVEAQLREEDLVRRPEKDA VPVASQWGSSQNPFEDAQISDLEASVESKCEPKPPVPVPRTPQTRAVKPRPHPVKPMNTTAPKITNSSLG TATIISENLINEALMKKYQPSDPAFAYAQLTHDELIQLVLKQKETISKKEFQVRELEDYIDNLLVRVMEE TPNILRVPAQTGKKAGKM SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001197241
ORF Size	1944 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001197241.1, NP_001184170.1
RefSeq Size	6225 bp
RefSeq ORF	1947 bp
Locus ID	498650
UniProt ID	Q3B7T9
MW	71.1 kDa
Gene Summary	A Rab11 effector protein involved in the endosomal recycling process. Also involved in controlling membrane trafficking along the phagocytic pathway and phagocytosis (By similarity).[UniProtKB/Swiss-Prot Function]

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