Crebrf (NM_001277157) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1310862
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217030 representing NM_001277157 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCAGCCCAGCGTAAGCGGAATGGATCCGCCTTTTGGGGATGCCTTTCGAAGCCACACCTTTTCAG AACAGACTCTGATGAGCACAGATCTCTTAGCCAACAGTTCTGATCCAGATTTCATGTATGAGCTGGATAG AGAGATGAATTATCAACAGAATCCTAGAGACAACTTCCTTTCTTTGGAAGACTGCAAAGACATTGAAAAT CTGGAGACTTTCACAGATGTCCTGGACAATGAGGATGCTTTGACTTCAAACTGGGAACAGTGGGATACAT ACTGTGAAGACTTAACTAAGTATACCAAACTAACCAGCTGTGACATCTGGGGGACAAAAGAAGTGGATTA CCTGGGTCTTGATGACTTTTCTAGCCCTTACCAAGATGAAGAGGTCATCAGTAAAACTCCAACCTTGGCC CAGCTCAATAGTGAGGACTCTCAGTCTGTTTCTGATTCCCTTTATTATCCTGATTCACTCTTCAGTGTCA AACAAAACCCCTTGCCCCCCTCTTCTTTTCCTAGTAAAAAGATCACAAGTAGAGCAGCTGCCCCTGTGTG CTCTTCAAAGATACTTCAGGCTGAGGTCCCATCGTCAGACTGTGTCCAAAAAGCAAGCAAACCTACTTCA AGCACACAGATCATGGTGAAGACCAACATGTATCATAATGAAAAAGTGAATTTTCATGTCGAATGTAAAG ACTATGTAAAAAAAGCAAAAGTCAAGATCAACCCTGTGCAACAGGGCCGGCCCTTGCTGAGCCAGGTGCA CATAGATGCGGCAAAGGAGAACACCTGCTACTGTGGCGCTGTGGTAAAGAGGCAGGAGAAGAGGGGGGTG GAGCCTCACCAGGGTCATGGCACTCCTGCTTTGCCTTTCAGAGAAACCCAGGAACTATTACTTAGTCCTC TGCCCCAGCATGGTCCTGGGTTGGTTGCCACAGCAGAGAGTGGCAGCCTTTCTGCCAGCACTTCTGTTTC AGATTCGTCCCAGAAAAAAGAAGAGCACAATTATTCTCTTTTTGTCTCTGACAACATGAGAGAACAGCCA ACCAAATACAGTCCTGAAGATGATGAGGATGATGAAGATGATATTGATGATGAGGACCATGATGAAGGGT TTGGCAGTGAGCATGAGCTTTCTGAAAATGAAGAGGAGGAAGAAGAGGAAGAGGATTATGAGGATGACAG AGATGATGATATCAGCGACACATTCTCTGAACCAGGCTATGAAAACGACTCTGTAGAGGACTTGAAGGAG ATGACTTCCATATCTTCTCGGAAGAGAGGGAAAAGAAGGTACTTCTGGGAGTATAGTGAGCAGTTGACGC CATCACAGCAAGAGAGGATTCTGAGGCCATCTGAGTGGAATCGAGACACCTTGCCAAGTAATATGTACCA GAAAAATGGCTTACATCATGGGAAATACGCAGTTAAGAAGTCACGGAGAACTGATGTGGAAGACCTGACT CCAAATCCTAAAAAACTACTCCAGATTGGTAATGAACTACGGAAACTGAATAAGGTGATCAGTGACCTGA CTCCAGTTAGTGAGCTTCCCTTAACAGCGAGGCCAAGGTCAAGGAAAGAAAAAAATAAGCTGGCTTCCAG AGCTTGTAGGCTGAAGAAGAAAGCCCAGTATGAAGCCAATAAAGTGAAGCTCTGGGGCCTCAACACTGAA TATGACAATTTATTATTTGTAATCAACTCCATCAAGCAAGACATTGTAAACCGAGTTCAGAATCCAAGAG AGGAGAGAGGACCCAGCATGGGGCAGAAGCTTGAAATCCTCATTAAAGACACACTCGGTCTCCCAGTCGC TGGGCAAACCTCAGAATTTGTTAACCAAGTGTTAGGGAAGACTGCTGAAGGCAACCCCACTGGAGGCCTT GTAGGACTAAGGATACCAGCATCAAAAGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217030 representing NM_001277157 Red=Cloning site Green=Tags(s) MPQPSVSGMDPPFGDAFRSHTFSEQTLMSTDLLANSSDPDFMYELDREMNYQQNPRDNFLSLEDCKDIEN LETFTDVLDNEDALTSNWEQWDTYCEDLTKYTKLTSCDIWGTKEVDYLGLDDFSSPYQDEEVISKTPTLA QLNSEDSQSVSDSLYYPDSLFSVKQNPLPPSSFPSKKITSRAAAPVCSSKILQAEVPSSDCVQKASKPTS STQIMVKTNMYHNEKVNFHVECKDYVKKAKVKINPVQQGRPLLSQVHIDAAKENTCYCGAVVKRQEKRGV EPHQGHGTPALPFRETQELLLSPLPQHGPGLVATAESGSLSASTSVSDSSQKKEEHNYSLFVSDNMREQP TKYSPEDDEDDEDDIDDEDHDEGFGSEHELSENEEEEEEEEDYEDDRDDDISDTFSEPGYENDSVEDLKE MTSISSRKRGKRRYFWEYSEQLTPSQQERILRPSEWNRDTLPSNMYQKNGLHHGKYAVKKSRRTDVEDLT PNPKKLLQIGNELRKLNKVISDLTPVSELPLTARPRSRKEKNKLASRACRLKKKAQYEANKVKLWGLNTE YDNLLFVINSIKQDIVNRVQNPREERGPSMGQKLEILIKDTLGLPVAGQTSEFVNQVLGKTAEGNPTGGL VGLRIPASKV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001277157
ORF Size	1920 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001277157.1, NP_001264086.1
RefSeq Size	6459 bp
RefSeq ORF	1923 bp
Locus ID	303016
MW	72.5 kDa

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