Mapkapk5 (NM_001164043) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR216750 representing NM_001164043 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGAGGATAGCAACATGGAGAAAGCCATCAAGGAGACCTCCATTTTAGAAGAATATAGTATCAATT GGACTCAGAAACTGGGAGCTGGAATTAGTGGTCCGGTTAGAGTCTGTGTAAAGAAATCTACTCAAGAACG GTTTGCACTGAAAATTCTTCTCGATCGTCCAAAAGCTAGAAATGAGGTGCGCCTGCACATGATGTGTGCC ACACACTCCAACATAGTTGAGATTATTGAAGTGTTTGCTAACAGTGTACAGTTCCCTCATGAGTCCAGCC CCAGGGCTCGACTCTTAATTGTAATGGAGATGATGGAAGGGGGCGAGCTGTTTCACAGAATCAGCCAGCA CCGGCACTTTACAGAGAAGCAAGCCAGCCAAGTAACAAAGCAGATAGCCCTGGCTCTACAGCACTGTCAC TTGCTAAACATTGCGCACAGAGACCTCAAGCCTGAAAATCTGCTTTTCAAGGATAACTCTTTGGACGCCC CTGTGAAGTTATGTGACTTTGGGTTTGCTAAAGTTGACCAAGGTGATTTGATGACGCCCCAGTTTACCCC TTACTATGTAGCACCTCAGGTACTGGAAGCACAGAGAAGGCACCAGAAGGAGAAGTCTGGCCTCATCCCT ACCTCGCCAACGCCCTACACTTACAACAAGAGCTGTGACTTGTGGTCCCTCGGGGTGATAATTTATGTGA TGCTATGCGGATACCCTCCTTTTTACTCCAAACACCACAGTCGGACCATCCCAAAGGACATGCGGAAAAA GATCATGACAGGGAGTTTTGAGTTCCCGGAGGAAGAGTGGAGCCAGATCTCAGAGATGGCCAAAGACGTG GTGAGGAAGCTTCTAAAGGTCAAACCAGAGGAAAGACTCACAATCGAGGGGGTGTTGGACCATCCCTGGC TCAACTCGACAGAGGCCCTGGATAATGTGCTACCCTCTGCTCAGCTGATGATGGATAAGGCAGTGGTTGC AGGGATCCAGCAGGCTCATGCCGAGCAGCTGGCAAACATGAGGATCCAGGACCTCAAAGTCAGCCTCAAA CCCCTGCACTCTGTGAACAACCCCATTCTGAGGAAGAGGAAGCTACTGGGCTCCAAGCCAAAGGATGGTG TTTATATCCACGACCATGAGAATGGAGTCGAGGATTCAAATGTTGCCTTGGAAAAGCTTCGCGATGTCAT TGCCCAGTGCATCCTTCCCCAGGCTGGTAAAGGAGAGAATGAAGATGAGAAGCTCAATGAGGTAATGCAG GAGGCCTGGAAGTACAACCGGGAGTGCAAACTCCTGCGGGACGCGCTGCAGAGTTTTAGCTGGAATGGTC GTGGATTCACAGATAAAGTGGACCGATTAAAGCTGGCAGAGGTTGTGAAGCAGGTGATCGAAGAGCAGAC CCTTCCCCACGAGCCCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR216750 representing NM_001164043 Red=Cloning site Green=Tags(s) MSEDSNMEKAIKETSILEEYSINWTQKLGAGISGPVRVCVKKSTQERFALKILLDRPKARNEVRLHMMCA THSNIVEIIEVFANSVQFPHESSPRARLLIVMEMMEGGELFHRISQHRHFTEKQASQVTKQIALALQHCH LLNIAHRDLKPENLLFKDNSLDAPVKLCDFGFAKVDQGDLMTPQFTPYYVAPQVLEAQRRHQKEKSGLIP TSPTPYTYNKSCDLWSLGVIIYVMLCGYPPFYSKHHSRTIPKDMRKKIMTGSFEFPEEEWSQISEMAKDV VRKLLKVKPEERLTIEGVLDHPWLNSTEALDNVLPSAQLMMDKAVVAGIQQAHAEQLANMRIQDLKVSLK PLHSVNNPILRKRKLLGSKPKDGVYIHDHENGVEDSNVALEKLRDVIAQCILPQAGKGENEDEKLNEVMQ EAWKYNRECKLLRDALQSFSWNGRGFTDKVDRLKLAEVVKQVIEEQTLPHEPQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001164043
ORF Size	1419 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001164043.1, NP_001157515.1
RefSeq Size	1699 bp
RefSeq ORF	1422 bp
Locus ID	498183
MW	54.1 kDa

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