Fkbp4 (NM_001191863) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	FKBP-4; FKBP-52; p59
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR216713 representing NM_001191863 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCGCCGAGGAGATGAAGGTGGCGGAGAACGGGGCGCAATCGGCGCCCCTGCCTCTCGAAGGAGTGG ACATCAGCCCCAAGCAGGACGAGGGCGTGCTCAAGGTCATCAAGAGAGAGGGTACAGGCACAGAGACAGC CATGATTGGGGACCGAGTCTTTGTCCACTACACTGGCTGGCTGCTAGATGGCACAAAGTTTGACTCCAGT TTGGACCGCAAGGACAAATTCTCCTTTGACCTGGGAAAAGGGGAGGTCATCAAGGCTTGGGATATTGCTG TGGCAACCATGAAAGTGGGGGAAGTGTGCCACATCACCTGCAAGCCAGAATACGCCTACGGTTCAGCAGG CAGCCCTCCAAAGATTCCCCCAAATGCCACACTTGTATTTGAGGTGGAGCTGTTTGAGTTCAAAGGAGAA GATCTTACAGAAGATGAAGATGGTGGGATCATCCGCAGAATACGGACTCGGGGGGAAGGCTATGCCAGGC CCAATGATGGCGCTATGGTAGAAGTGGCACTAGAAGGCTACTATAATGACCGGCTGTTTGACCAGCGGGA GCTCTGCTTTGAAGTCGGGGAAGGGGAAAGCCTGGATCTGCCCTGTGGGCTGGAGGAGGCCATTCAGCGC ATGGAGAAAGGAGAACATTCCATTGTGTACCTCAAACCTAGCTATGCTTTTGGCAGTGTTGGGAAGGAAA GGTTCCAGATCCCACCACATGCTGAGCTGAGGTATGAAGTACATCTGAAGAGTTTTGAGAAGGCCAAGGC ATCTTGGGAGATGAACTCTGAGGAGAAGCTGGAGCAGAGCAACATAGTGAAAGAAAGGGGCACCGTGTAC TTCAAGGAAGGCAAGTACAAGCAAGCCTTACTGCAGTACAAGAAGATTGTGTCTTGGCTAGAATACGAGT CTAGTTTTTCCGGTGAGGAAATGCAAAAGGTCCATGCGCTCCGACTGGCCTCACATCTCAATCTGGCCAT GTGTCACCTGAAACTGCAGGCCTTCTCAGCTGCCATCGAAAGCTGTAACAAGGCCTTGGAGCTGGACAGC AACAATGAGAAGGGCCTGTTTCGCCGGGGAGAGGCACACCTGGCCGTGAATGACTTTGACCTGGCACGAG CTGACTTCCAAAAGGTCCTGCAGCTCTATCCCAGCAACAAAGCCGCCAAGACCCAGCTGGCTGTGTGCCA GCAGCGGACCCGCAGGCAGCTTGCCCGGGAAAAGAAGCTCTATGCCAACATGTTTGAGAGGCTGGCTGAG GAGGAACATAAGGCCAAGACAGAAGTGGCTGCAGGAGACCATCCCACTGATGCTGAGATGAAGGGTGAGC CGAACAATGTGGCAGGCAACCAGGCTCAGGTGAAGACAGAAGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR216713 representing NM_001191863 Red=Cloning site Green=Tags(s) MTAEEMKVAENGAQSAPLPLEGVDISPKQDEGVLKVIKREGTGTETAMIGDRVFVHYTGWLLDGTKFDSS LDRKDKFSFDLGKGEVIKAWDIAVATMKVGEVCHITCKPEYAYGSAGSPPKIPPNATLVFEVELFEFKGE DLTEDEDGGIIRRIRTRGEGYARPNDGAMVEVALEGYYNDRLFDQRELCFEVGEGESLDLPCGLEEAIQR MEKGEHSIVYLKPSYAFGSVGKERFQIPPHAELRYEVHLKSFEKAKASWEMNSEEKLEQSNIVKERGTVY FKEGKYKQALLQYKKIVSWLEYESSFSGEEMQKVHALRLASHLNLAMCHLKLQAFSAAIESCNKALELDS NNEKGLFRRGEAHLAVNDFDLARADFQKVLQLYPSNKAAKTQLAVCQQRTRRQLAREKKLYANMFERLAE EEHKAKTEVAAGDHPTDAEMKGEPNNVAGNQAQVKTEA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191863
ORF Size	1374 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191863.1, NP_001178792.1
RefSeq Size	2845 bp
RefSeq ORF	1377 bp
Locus ID	260321
UniProt ID	Q9QVC8
MW	51.5 kDa
Gene Summary	Immunophilin protein with PPIase and co-chaperone activities (By similarity). Component of unligated steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90) (By similarity). Plays a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments. May have a protective role against oxidative stress in mitochondria (By similarity). Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly. The PPIase activity controls neuronal growth cones via regulation of TRPC1 channel opening.[UniProtKB/Swiss-Prot Function]

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