Becn1 (NM_001034117) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Beclin1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR216694 representing NM_001034117 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGGGTCTAAGGCGTCCAGCAGCACCATGCAGGTGAGCTTCGTGTGCCAGCGCTGTAGCCAGCCTC TGAAACTGGACACGAGCTTCAAGATCCTGGACCGAGTGACCATTCAGGAACTCACAGCTCCATTACTTAC CACAGCCCAGGCGAAACCAGGAGAGAGCCAGGAGGAAGAGGCTAACTCAGGAGAGGAGCCATTTATTGAA ACTCGCCAGGATGGTGTCTCTCGAAGATTCATCCCCCCAGCCAGGATGATGTCTACAGAAAGTGCTAATA GCTTCACTCTGATCGGGGAGGCATCTGATGGTGGCACCATGGAGAACCTCAGCCGGAGACTCAAGGTCAC TGGAGACCTTTTTGACATCATGTCTGGCCAGACAGATGTGGATCACCCACTGTGTGAGGAATGCACAGAT ACTCTTTTAGACCAGCTGGACACTCAGCTCAATGTTACTGAAAACGAGTGTCAGAACTACAAACGCTGTT TGGAGATGTTGGAGCAAATGAATGAGGGCGACAGTGAACAGCTACAGAGGGAGCTGAAGGAGTTGGCCTT GGAGGAGGAGAGGCTGATCCAGGAGCTGGAAGATGTGGAAAAAAACCGAAAGGTGGTGGCAGAAAACCTG GAGAAGGTCCAGGCTGAGGCGGAGAGACTGGACCAGGAGGAAGCTCAGTACCAGCGAGAATATAGTGAAT TTAAAAGGCAGCAGCTGGAGCTGGATGATGAGCTCAAGAGTGTAGAGAACCAGATGCGCTATGCCCAGAT GCAGCTGGACAAGCTCAAGAAAACCAATGTCTTCAATGCGACCTTCCATATCTGGCACAGCGGACAATTT GGCACGATCAATAATTTCAGACTGGGTCGCTTGCCCAGTGCTCCTGTGGAATGGAATGAAATCAATGCTG CCTGGGGCCAGACAGTGTTGTTGCTCCATGCTTTGGCCAATAAGATGGGTCTGAAATTTCAGAGGTACCG ACTTGTTCCCTATGGAAATCACTCGTATCTGGAGTCCCTGACAGACAAATCTAAGGAGTTGCCGTTGTAC TGTTCTGGGGGTTTGCGGTTTTTCTGGGACAACAAGTTTGACCATGCAATGGTAGCTTTTCTGGACTGTG TGCAGCAGTTCAAAGAAGAGGTGGAAAAAGGAGAGACTCGATTTTGTCTTCCGTACAGGATGGACGTGGA GAAAGGCAAGATTGAAGACACTGGAGGCAGTGGCGGCTCCTATTCCATCAAAACCCAGTTTAACTCTGAG GAGCAGTGGACAAAGGCGCTCAAGTTCATGCTGACGAATCTCAAGTGGGGTCTTGCTTGGGTGTCCTCAC AGTTCTATAACAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR216694 representing NM_001034117 Red=Cloning site Green=Tags(s) MEGSKASSSTMQVSFVCQRCSQPLKLDTSFKILDRVTIQELTAPLLTTAQAKPGESQEEEANSGEEPFIE TRQDGVSRRFIPPARMMSTESANSFTLIGEASDGGTMENLSRRLKVTGDLFDIMSGQTDVDHPLCEECTD TLLDQLDTQLNVTENECQNYKRCLEMLEQMNEGDSEQLQRELKELALEEERLIQELEDVEKNRKVVAENL EKVQAEAERLDQEEAQYQREYSEFKRQQLELDDELKSVENQMRYAQMQLDKLKKTNVFNATFHIWHSGQF GTINNFRLGRLPSAPVEWNEINAAWGQTVLLLHALANKMGLKFQRYRLVPYGNHSYLESLTDKSKELPLY CSGGLRFFWDNKFDHAMVAFLDCVQQFKEEVEKGETRFCLPYRMDVEKGKIEDTGGSGGSYSIKTQFNSE EQWTKALKFMLTNLKWGLAWVSSQFYNK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001034117
ORF Size	1344 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001034117.1, NP_001029289.1
RefSeq Size	2118 bp
RefSeq ORF	1347 bp
Locus ID	114558
UniProt ID	Q91XJ1
MW	51.6 kDa
Gene Summary	mouse homolog is a haploinsufficient tumor suppressor gene; plays a role in early embryonic development [RGD, Feb 2006]

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