Eno1 (NM_012554) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Nne
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR216663 representing NM_012554 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCATTCTCAAGATCCATGCCAGAGAGATCTTTGACTCCCGCGGGAATCCCACCGTTGAGGTGGATC TCTACACCGCAAAAGGTCTCTTCCGTGCTGCGGTGCCCAGCGGTGCGTCCACTGGCATCTACGAGGCCCT AGAACTCCGAGACAATGATAAGACCCGCTTCATGGGGAAGGGTGTCTCAAAGGCTGTTGAGCACATCAAT AAAACTATTGCACCTGCTCTGGTTAGCAAGAAACTGAATGTTGTGGAGCAGGAGAAGATTGACCAGCTGA TGATCGAGATGGACGGCACAGAGAATAAATCTAAGTTTGGTGCAAATGCCATCCTGGGAGTGTCCCTGGC TGTCTGCAAGGCTGGTGCCGTGGAGAAGGGGGTGCCCCTTTACCGTCACATTGCCGACTTGGCCGGCAAC CCTGAAGTCATCCTGCCGGTCCCAGCTTTCAATGTGATCAACGGCGGTTCTCATGCTGGCAACAAGTTGG CCATGCAAGAGTTCATGATCCTGCCTGTGGGGGCATCCTCTTTCCGGGAAGCCATGCGCATTGGAGCAGA GGTTTACCACAACCTGAAGAACGTCATCAAGGAGAAGTACGGGAAAGACGCCACCAATGTGGGTGATGAG GGTGGATTCGCACCTAACATCCTGGAGAACAAAGAAGCACTGGAGCTGCTCAAGTCTGCCATTGCAAAGG CCGGCTACACTGACCAGGTTGTCATCGGCATGGATGTGGCTGCCTCCGAGTTCTACAGGGCTGGCAAGTA TGACCTGGACTTCAAGTCTCCAGATGATGCCAGCCGGTACATCACACCCGACCAGCTGGCCGACCTGTAC AAGTCCTTCATCAAGGACTACCCAGTGGTGTCCATTGAAGATCCCTTTGACCAGGACGACTGGGATGCTT GGCAGAAGTTCACAGCTACTGCAGGCATCCAGGTGGTGGGGGATGACCTCACAGTGACCAACCCTAAGCG GATCGCCAAGGCTGCAGGCGAAAAGTCCTGCAACTGCCTCCTGCTCAAAGTGAACCAGATTGGCTCTGTG ACCGAGTCTCTGCAGGCGTGTAAGCTGGCCCAGTCCAATGGCTGGGGTGTCATGGTGTCCCATCGATCTG GGGAGACTGAGGACACTTTCATTGCCGACCTGGTGGTGGGGCTCTGCACTGGGCAGATCAAGACTGGTGC CCCCTGCCGATCTGAGCGCCTGGCCAAGTACAATCAGATCCTTAGAATCGAGGAGGAGCTGGGCAGCAAA GCCAAGTTTGCCGGCAGGTCCTTCAGGAACCCCCTGGCCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR216663 representing NM_012554 Red=Cloning site Green=Tags(s) MSILKIHAREIFDSRGNPTVEVDLYTAKGLFRAAVPSGASTGIYEALELRDNDKTRFMGKGVSKAVEHIN KTIAPALVSKKLNVVEQEKIDQLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGN PEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDE GGFAPNILENKEALELLKSAIAKAGYTDQVVIGMDVAASEFYRAGKYDLDFKSPDDASRYITPDQLADLY KSFIKDYPVVSIEDPFDQDDWDAWQKFTATAGIQVVGDDLTVTNPKRIAKAAGEKSCNCLLLKVNQIGSV TESLQACKLAQSNGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQILRIEEELGSK AKFAGRSFRNPLAK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_012554
ORF Size	1302 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_012554.3, NP_036686.2
RefSeq Size	1754 bp
RefSeq ORF	1305 bp
Locus ID	24333
UniProt ID	P04764
MW	47.1 kDa
Gene Summary	Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.[UniProtKB/Swiss-Prot Function]

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