Ppme1 (NM_001191838) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	PME-1; RGD1309683
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR216537 representing NM_001191838 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGCCCTTGAAAAAAGCATGCACCTCGGCCGCTTACCTTCTCGCCCTCCTCTACCCGGCAGCGGGG GCAGTCAGAGCGGAGCCAAGATGCGGATGGGCCCTGGAAGGAAGCGGGACTTTACCCCTGTTCCATGGAG TCAGTACTTCGAGTCAATGGAAGATGTGGAGGTGGAGAATGAAACTGGCAAGGATACTTTTCGAGTTTAC AAGAGTGGTTCCGAGGGTCCAGTCCTGCTGCTGCTTCATGGAGGAGGCCATTCTGCCCTTTCCTGGGCAG TATTCACGGCAGCAATCATCAGTAGAGTACAGTGTCGGATTGTGGCTCTGGATCTACGAAGCCATGGAGA AACTAAAGTCAAGAATTCTGAAGACCTGTCAGCAGAAACAATGGCAAAAGATGTTGGTAATGTGGTGGAA GCCATGTATGGCGACCTCCCTCCTCCCGTCATGCTGATTGGGCACAGCATGGGCGGTGCCATTGCAGTAC ACACAGCAGCAGCTAACCTGGTGCCAAGCCTTTTGGGTCTTTGTATGATTGATGTTGTGGAAGGTACAGC CATGGATGCCCTTAATAGCATGCAGAATTTCTTACGGGGTCGGCCCAAAACCTTCAAGTCTCTGGAGAAT GCTATTGAATGGAGTGTGAAGAGTGGCCAGATTCGAAATCTAGAATCTGCCCGTGTATCCATGGTCGGCC AAGTCAAACAGTGTGAAGGAATTACAAGTCCAGAAAGTTCCAAATCCATAGTAGAAGGAATCATAGAGGA GGAGGAAGAAGATGAAGAAGGAAGTGAGTCAGTTAACAAGAGGAAAAAGGAAGACGACATGGAAACCAAG AAGGATCATCCATACACCTGGAGAATTGAGCTGGCAAAAACAGAGAAGTACTGGGACGGCTGGTTCCGAG GCTTATCCAATCTCTTTCTTAGCTGTCCTATTCCTAAACTGCTGCTCTTGGCAGGTGTTGATAGATTGGA TAAAGATCTGACCATAGGCCAGATGCAGGGGAAGTTCCAGATGCAGGTCTTACCCCAGTGTGGCCATGCA GTCCATGAGGATGCCCCTGACAAGGTAGCTGAAGCTGTTGCCACTTTCCTGATCCGACACAGGTTTGCAG AGCCCATCGGAGGATTCCAGTGTGTGTTTCCTGGCTGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR216537 representing NM_001191838 Red=Cloning site Green=Tags(s) MSALEKSMHLGRLPSRPPLPGSGGSQSGAKMRMGPGRKRDFTPVPWSQYFESMEDVEVENETGKDTFRVY KSGSEGPVLLLLHGGGHSALSWAVFTAAIISRVQCRIVALDLRSHGETKVKNSEDLSAETMAKDVGNVVE AMYGDLPPPVMLIGHSMGGAIAVHTAAANLVPSLLGLCMIDVVEGTAMDALNSMQNFLRGRPKTFKSLEN AIEWSVKSGQIRNLESARVSMVGQVKQCEGITSPESSKSIVEGIIEEEEEDEEGSESVNKRKKEDDMETK KDHPYTWRIELAKTEKYWDGWFRGLSNLFLSCPIPKLLLLAGVDRLDKDLTIGQMQGKFQMQVLPQCGHA VHEDAPDKVAEAVATFLIRHRFAEPIGGFQCVFPGC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191838
ORF Size	1158 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191838.1, NP_001178767.1
RefSeq Size	2436 bp
RefSeq ORF	1161 bp
Locus ID	361613
UniProt ID	Q4FZT2
MW	42.3 kDa
Gene Summary	Demethylates proteins that have been reversibly carboxymethylated. Demethylates PPP2CB (in vitro) and PPP2CA. Binding to PPP2CA displaces the manganese ion and inactivates the enzyme (By similarity).[UniProtKB/Swiss-Prot Function]

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