Selenop (NM_001083911) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Sepp1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR216536 representing NM_001083911 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGAGAAGCCTAGGGCTTGCCCTGGCTCTCTGTCTCCTCCCCTATGGAGGAGCAGAGAGCCAAGGCC AAAGCCCTGCTTGTAAGCAAGCTCCACCCTGGAACATAGGAGATCAAAATCCAATGCTAAACTCCGAGGG CACAGTGACAGTGGTTGCTCTTCTTCAAGCCAGCTGATACCTGTGCCTTCTGCAGGCATCCAGATTGGAA GACCTGCGAATAAAACTAGAGAACCAAGGATATTTTAACATCTCCTATATTGTTGTTAATCATCAAGGAT CTCCTTCCCAATTAAAACATGCACATCTTAAAAAGCAGGTGTCAGATCACATTGCTGTTTACAGACAAGA TGAACATCAAACAGATGTCTGGACACTCTTAAATGGAAACAAAGATGACTTCCTCATATATGACAGATGT GGCCGTCTTGTGTATCACCTTGGTTTGCCCTACTCCTTCCTCACTTTCCCGTATGTTGAAGAAGCCATCA AGATCGCTTACTGTGAGAAGAGGTGTGGAAACTGCTCTTTCACGAGTCTTGAAGATGAAGCCTTCTGTAA AAACGTGTCCTCGGCTACTGCAAGTAAAACCACAGAGCCCTCAGAGGAGCATAACCACCACAAGCACCAT GACAAACATGGGCATGAGCATCTTGGGAGCAGTAAGCCTTCAGAGAATCAGCAACCAGGGGCATTAGATG TTGAGACAAGTCTTCCTCCTTCAGGCTTGCACCACCACCACCACCACCATAAGCACAAGGGCCAGCACAG GCAGGGTCACTTAGAGAGCTGAGACATGGGGGCAAGTGAAGGCTTGCAACTTTCACTTGCCCAGAGGAAG CTCTGACGAAGGGGATGCATAAACCAGCTCCTGTGTAAGTTATCTGAGGAGTCTGGGGCAGCTACCAGTA GCTGCTGCTGCCACTGCCGACACCTCATATTTGAGAAGTCAGGATCTGCAATCACTTGACAGTGTGCCGA AAACCTCCCATCCTTGTGTAGCTGACAGGGGCTTTTCGCGGAGGAGAAAGTCATTGAATCCTGTCAATGT AGATCACCTCCAGCTGCCTGACACAGTCAGCATGTAAGCCCCACAGAAGCCAGCCCCAACTGAAGCTGAA ATAATAAGACCAAGAAGTGAAAATGAAATTTGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR216536 representing NM_001083911 Red=Cloning site Green=Tags(s) MWRSLGLALALCLLPYGGAESQGQSPACKQAPPWNIGDQNPMLNSEGTVTVVALLQAS*YLCLLQASRLE DLRIKLENQGYFNISYIVVNHQGSPSQLKHAHLKKQVSDHIAVYRQDEHQTDVWTLLNGNKDDFLIYDRC GRLVYHLGLPYSFLTFPYVEEAIKIAYCEKRCGNCSFTSLEDEAFCKNVSSATASKTTEPSEEHNHHKHH DKHGHEHLGSSKPSENQQPGALDVETSLPPSGLHHHHHHHKHKGQHRQGHLES*DMGASEGLQLSLAQRK L*RRGCINQLLCKLSEESGAATSSCCCHCRHLIFEKSGSAIT*QCAENLPSLCS*QGLFAEEKVIESCQC RSPPAA*HSQHVSPTEASPN*S*NNKTKK*K*NLN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001083911
ORF Size	1155 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001083911.1, NP_001077380.1
RefSeq Size	2115 bp
RefSeq ORF	1158 bp
Locus ID	29360
UniProt ID	P25236
MW	42.8 kDa
Gene Summary	This gene encodes a selenoprotein that is predominantly expressed in the liver and secreted into the plasma. This selenoprotein is unique in that it contains multiple selenocysteine (Sec) residues per polypeptide (10 in rat), and accounts for most of the selenium in plasma. It has been implicated as an extracellular antioxidant, and in the transport of selenium to extra-hepatic tissues via apolipoprotein E receptor-2 (apoER2). Mice lacking this gene exhibit neurological dysfunction, suggesting its importance in normal brain function. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. The mRNA for this selenoprotein contains two SECIS elements. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2017]

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