Bag1 (NM_001256084) Rat Tagged ORF Clone
CAT#: RR216075
- TrueORF®
Bag1 (myc-DDK-tagged) - Rat BCL2-associated athanogene (Bag1), transcript variant 1
"NM_001256084" in other vectors (1)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Bag-1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR216075 representing NM_001256084
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCAGACCGAGGAGATGGTCCAGACGGAGGAAATGGAACCACCCACACTCAGCGTGGTCGTCACCC ACAGCAATGAGAGGTATGACCTTCTTGTTACCCCACAGCAAGGTAACAGTGAGCCAATTGTCCAAGACCT GGCTCAGCTTGTTGAAGAGGCCACAGGAGTTCCACTACCTTTTCAGAAGCTCATATTTAAGGGCAAATCT CTGAAAGAAATGGAAACACCCTTGTCAGCACTTGGAATGCAAAATGGTTGCCGAGTCATGTTAATTGGTG AAAAGAGCAATCCAGAAGAAGAGGCTGAGTTGAAAAAGCTGAAGGACTTGGAGGTATCTGTGGAGAAGAC AGCTAACCACCTGGAAGAGTTGAATAAAGAGCTTTCTGACATCCAGCAGGGTTTTCTGGCTAAGGAATTA CAAGCGGAGGCTCTCTGCAGACTTGATAGGAAAATAAAGGCCACAATTGAGCAATTCATGAAGATCTTGG AGGAGATTGACACAATGGTCCTACCAGAAAACTTTAAAGACAGCAGGCTAAAAAGGAAGAATTTGGTGAA AAAGGTTCAGGTGTTCCTAGCAGAGTGTGATACAGTGGAGCAGTACATCTGCCAAGAGACAGAGCGGCTG CAGTCTACAAACTTGGCCCTGCCTGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR216075 representing NM_001256084
Red=Cloning site Green=Tags(s) MAQTEEMVQTEEMEPPTLSVVVTHSNERYDLLVTPQQGNSEPIVQDLAQLVEEATGVPLPFQKLIFKGKS LKEMETPLSALGMQNGCRVMLIGEKSNPEEEAELKKLKDLEVSVEKTANHLEELNKELSDIQQGFLAKEL QAEALCRLDRKIKATIEQFMKILEEIDTMVLPENFKDSRLKRKNLVKKVQVFLAECDTVEQYICQETERL QSTNLALPE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001256084 |
ORF Size | 657 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001256084.1, NP_001243013.1 |
RefSeq Size | 1308 bp |
RefSeq ORF | 660 bp |
Locus ID | 297994 |
UniProt ID | B0K019 |
MW | 24.9 kDa |
Gene Summary | The oncogene Bcl2 encodes a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. Studies in human and mouse suggest that the protein encoded by this gene (referred to as Bcl2-associated athanogene) binds to Bcl2 protein. It enhances the anti-apoptotic effects of Bcl2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. At least two protein isoforms are encoded by this mRNA through the use of a non-AUG (CUG) start site and an alternative, downstream, AUG translation initiation site. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN216075 | Bag1 (untagged) - Rat BCL2-associated athanogene (Bag1), transcript variant 1 |
CNY 2,190.00 |