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Ngef (NM_001136241) Rat Tagged ORF Clone
CAT＃: RR215599
- TrueORF®
Ngef (Myc-DDK-tagged ORF) - Rat neuronal guanine nucleotide exchange factor (Ngef), (10 ug)
"NM_001136241" in other vectors (3)
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CNY 7,890.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Besh3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR215599 representing NM_001136241
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTGCTGGCTGCCGCTTTCAGCGCTGCCTGCGCCGTGGACCACGACAGCTCCACCTCGGAGAGCG ACACGCGCGACTCGGCGGCGGGACACCTGCCCGGCAGCGAGTCATCCTCCACCCCTGGAAATGGAACCAC ACCCGAGGAGTGCCCGGCCCTCACCGACAGCCCCACCACGCTCACCGAGGCCCTGCAGATGATCCATCCC ATTCCCGCCGACTCCTGGAGAAACCTCATTGAACAAATAGGGCTCCTGTACCAAGAGTATAGAGACAAAT CGACTCTCCAAGAGATCGAAACCCGGAGGCAACAGGATGCAGAAATACAAGGCAACTCGGATGGGTCCCA GGCTGGGGAAGACAATGCAGAGGAGGAGGAAGAGGAGGAGGAGGAGCCGGCCAGCCCCCCTGAGAGGAGA GCTCTGCCTCAGATCTGCCTGCTCAGCAACCCCCACTCCAGGTTCAACCTCTGGCAGGACCTTCCTGAGA TCCAGAGCAGCGGGGTGCTGGACATCCTCCAGCCTGAGGAGACCAAGCTGCAGGAGGCCATGTTTGAGTT GGTCACCTCTGAGGCCTCCTACTATAAGAGCCTGAACCTGCTGGTGTCGCACTTCATGGAGAACGAGCGG CTGAAGAAGATCCTGCACCCGTCCGAGGCCCACATCCTCTTCTCCAATGTCCTGGATGTCATGGCTGTCA GTGAGCGGTTTTTGCTAGAGCTGGAACACCGCATGGAGGAGAACATTGTCATCTCGGATGTGTGTGACAT CGTGTACCGTTATGCGGCTGATCACTTCTCGGTCTATATCACCTACGTCAGTAACCAGACCTACCAGGAA AGGACGTACAAGCAACTCCTACAGGAGAAGACGGCTTTTCGGGAACTGATCGCACAGCTGGAGCTGGACC CCAAGTGCAAGGGCCTGCCCTTGTCCTCCTTCCTCATCTTGCCCTTCCAGAGGATCACGAGACTCAAGCT GCTGGTCCAGAACATCCTGAAGAGGGTGGAGGAAGGGTCTGAACGTGAAGGCACTGCCTTGGATGCCCAC AAGGAGCTAGAAATGGTGGTAAAGGCGTGCAACGAGGGTGTCCGGAAGATGAGCCGCACAGAACAGATGA TCAGCATTCAGAAGAAGATGGAGTTCAAGATCAAGTCGGTACCTATCATCTCACACTCCCGGTGGCTGCT GAAGCAAGGTGAACTACAGCAGATGTCCGGCCCCAAGACCTCCCGCACCCTGCGGACCAAGAAGCTCTTC AGAGAGATTTACCTCTTCCTCTTCAATGACCTGCTGGTGATCTGCCGGCAGATCCCTGGAGACAAGTACC AGGTGTTCGATTCCGCTCCAAGGGGCCTGCTGCGAGTGGAGGAGCTGGAGGACCAGGGCCAGACACTGGC TAATGTGTTCATCCTGCGGCTGCTGGAAAACGCAGATGACCGGGAGGCCACCTATATGCTGAAGGCATCC TCCCAGAGCGAGATGAAGCGTTGGATGACTTCACTGGCCCCCAACAGGAGGACCAAGTTTGTATCCTTCA CATCTCGGCTGTTGGACTGTCCGCAGGTCCAGTGTGTCCACCCCTACGTGGCCCAGCAGCCCGATGAGCT GACACTGGAACTGGCAGACATCCTGAACATCCTAGAGAAGACGGAGGATGGGTGGATCTTTGGTGAGCGG CTGCATGACCAGGAGAGAGGCTGGTTCCCCAGTTCCATGACAGAGGAGATCCTGAACCCCAAGATCCGCT CCCAAAACCTCAAGGAATGTTTCCGAGTGCATAAGATGGAGGACCCTCAGCGCAGCCAGAATAAGGACCG CAGGAAGTTGGGCAGCCGGAATCGTCAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR215599 representing NM_001136241
Red=Cloning site Green=Tags(s) MELLAAAFSAACAVDHDSSTSESDTRDSAAGHLPGSESSSTPGNGTTPEECPALTDSPTTLTEALQMIHP IPADSWRNLIEQIGLLYQEYRDKSTLQEIETRRQQDAEIQGNSDGSQAGEDNAEEEEEEEEEPASPPERR ALPQICLLSNPHSRFNLWQDLPEIQSSGVLDILQPEETKLQEAMFELVTSEASYYKSLNLLVSHFMENER LKKILHPSEAHILFSNVLDVMAVSERFLLELEHRMEENIVISDVCDIVYRYAADHFSVYITYVSNQTYQE RTYKQLLQEKTAFRELIAQLELDPKCKGLPLSSFLILPFQRITRLKLLVQNILKRVEEGSEREGTALDAH KELEMVVKACNEGVRKMSRTEQMISIQKKMEFKIKSVPIISHSRWLLKQGELQQMSGPKTSRTLRTKKLF REIYLFLFNDLLVICRQIPGDKYQVFDSAPRGLLRVEELEDQGQTLANVFILRLLENADDREATYMLKAS SQSEMKRWMTSLAPNRRTKFVSFTSRLLDCPQVQCVHPYVAQQPDELTLELADILNILEKTEDGWIFGER LHDQERGWFPSSMTEEILNPKIRSQNLKECFRVHKMEDPQRSQNKDRRKLGSRNRQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001136241 |
| ORF Size | 1848 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001136241.1, NP_001129713.1 |
| RefSeq Size | 2675 bp |
| RefSeq ORF | 1851 bp |
| Locus ID | 246217 |
| MW | 70.8 kDa |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN215599 | Ngef (untagged ORF) - Rat neuronal guanine nucleotide exchange factor (Ngef), (10 ug) |
CNY 7,890.00 |
|
| RR215599L3 | Lenti ORF clone of Ngef (Myc-DDK-tagged ORF) - Rat neuronal guanine nucleotide exchange factor (Ngef), (10 ug) |
CNY 9,790.00 |
|
| RR215599L4 | Lenti ORF clone of Ngef (mGFP-tagged ORF) - Rat neuronal guanine nucleotide exchange factor (Ngef), (10 ug) |
CNY 10,640.00 |
