Cep290 (NM_001135755) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1311640
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR215476 representing NM_001135755 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCACCTAATATAAAATGGAAAGAATTAATCAAAGTTGATCCAGATGAACTGCCACGTCAAGAAGAGT TGGCAGATAAATTATTGATATCCTTATCCAAGGTGGAAGTAAATGAACTAAAAAATGAAGATCAAGAAAA CATGATACATCTGTTCAGAATTACTCAGTCACTAATGAAGATGAAAGCTCAAGAAGTGGAGCTGGCCTTG GAAGAAGTTGAAAAGGCTGGGGAAGAACAAGCAAAATTTGAAAATCAATTAAAGACAAAAGTAATGAAAC TGGAAAATGAACTGGAGATGGCTCAGCAGTCTGCAGGTGGACGTGACACTCGGTTTTTACGTGATGAAAT TCGCCAACTTGAAAAGCAGTTGGAACAAAAAGATAGAGAATTAGAGGATATGGAAAAAGAACTGGATAAA GAAAAGAAAGTTAATGAACAATTGGCCCTTCGAAACGAGGAGGCAGAAAATGAAAACAGCAAATTAAGAA GAGAGAACAAACGTCTAAAGAAAAAGAATGAACAGCTTCGTCAGGACATTATTGACTACCAGAAACAAAT AGATTCACAAAAAGAATCACTTCTGTCAAGGAGAGGAGAGGACAGTGACTACCGATCACAGTTATCTAAA AAGAACTATGAACTTGTTCAATATCTGGACGAAATACAGACCTTAACAGAAGCTAATGAGAAAATTGAAG TTCAGAACCAAGAAATGAGAAAAAACCTAGAAGAGTCTGTACAGGAAATGGAGAAGATGACTGATGAATA TAACAGGATGAAAGCGATTGTGCATCAGACAGATACTGTTATGGACCAGATCAAAAAAGAGAATGAGCAC TACCGCCTTCAAGTGCGAGAACTCACAGATCTTCTGAAAGCAAAGGATGAAGAGGATGATCCCGTCATGA TGGCTGTCAATGCAAAAGTGGAGGAATGGAAGTTAATTTTGTCTTCTAAAGATGATGAAATCATTGAATA CCAGCAAATGTTACAGAGTCTGAGAGGGAAACTTAAAAATGCCCAGCTTGATGCTGATAAAAGTAACATT ATGGCTCTGAAGCAGGGTATCCAGGAACGAGACAGTCAAATTAAGATGCTTACTGAACAAGTAGAACAGT ATACGAAAGAAATGGAAAAAAATACTTTTATTATTGAAGATTTGAAAAATGAGCTCCGAAAAGACAAAGG TACTTCAAACATTTATCAGCAGACTCATTATATGAAAATCCACTCAAAGGTACAAATTTTAGAAGAGAAA ACAAAAGAGGCCGAGAGAACAGCTGAGCTGGCTGAGGCTGATGCTAGGGAGAAGGACAAAGAATTGGTTG AGGCTCTGAAGAGATTAAAAGATTATGAATCTGGAGTATATGGCTTAGAAGATGCGGTTATTGAAATAAA GAATTATAAAGCCCAAATTAAAATAAGAGATGGAGAGATTGAAGTGTTGACCAAGGAGATCAATAAGCTT GAGATGAAGATCAATGATGTCCTTGATGAAAATGAAGCTCTTAGAGAGCGGGCCGGCCTTGAACCCAAGA CAATGATTGATCTGACTGAATTTAGAAATAGTAAACGCGTAAAGCAGCAGCAGTACAGAGCTGAGAACCA GATTCTTTTGAAAGAGATCGAAAGTCTAGAGGAAGAACGTCTTGACTTGAAGAGAAAAATTCGTCAAATG GCTCAAGAAAGAGGCAAAAGGAGTGCAGCCTCAGGATTAACCATTGATGATCTGAACCTAACTGAAACCT TTTCTCACGGGGATAAAATAGGAGAAAGAAAGCTAAATTTTATAAGCTTCAATAATATGAATGAAACACA ATCAAAGAATGAATTTCTTTCAAGAGAATTGATTGAAAAGGAAAAAGATTTAGAAAGAAGTAGGACAGTA ATTACTAAATTTCAGAATAAACTAAAAGAATTAGTTGAAGAAAATAAGCAACTTGAAGAAGGTATGAAGG AAATTTTGCAAGCCATTAAGGACATGCCAAAGGATTCTGATGTGAAAGGAAGTGAAACATCTTTAATCAT CCCAAGTCTTGAAAGACTGGTTAATGCTATGGAATCAAAGAATGCAGAAGGAATCTTCGATGCTAGTTTG CATCTGAAAGCCCAAGTTGATCAACTTACAGGAAGGAATGAAGAATTAAGGCAAGAACTCAGGGAATCTC GGAAAGAAGCAGTAAATTATTCCCAGCAGTTGGTAAAAGCAAACTTAAAGATTGATCACCTTGAGAAAGA AACAGACCTTCTGCGTCAATCTTCAGGCTCCAATGTAGTTTACAAAGGCATAGACTTACCTGACGGGATA GCACCATCTAGTGCCTACATCATCAACTCTCAGAATGAGTATTTAATACATCTTTTGCAGGAACTAGACA ATAAAGAAAAGAAATTAAAGCACTTAGAAGATTCTCTTGAAGATTATAACAGAAAATTTGCAGTGATTCG ACATCAACAAAGTTTATTATATAAAGAATATCTAAGTGAAAAGGATGTTTGGAAAGCAGATTCTGAGATG ATAAAAGAGGAGAAGAGAAAGCTGGAGGATCAGGCTCAGCAGGACGCTGTGAAAGTAAAAGAGTATAATA ATTTGCTCAATGCCCTTCAGATGGACTCGAATGAAATGAAGAAAATGCTTTCAGAAAACAGTAGGAAAAT CACGGTTCTGCAAGTGAATGAGAAGTCCCTCATCCGGCAGTATACAACCTTAGTGGAGATAGAGCGGCAA CTTAGAAAAGAAAACGGGAAACACAAGAATGACGTCATATCCATGGAAGCCGAAGTTACTGAAAAAATTG GAAATTTGCAGAGATTTAAGGAAATGGCCACCTTCAGGATTGCAGCTCTTCAGAAAGTTGTAGATAACAG TGTTTCTTTGTCTGAACTAGAACTAGCCAATAAACAGTACAGTGAGCTGACTGCGAAGTACAGGGACGTC TTGCAGAAAGATAACATGCTTGTTCAGAGAGCAAGTAACTTAGAGCACCTAGCGTGTGAAAATGCATCGC TGAAAGAACAGATGGAGGGGCTCAGTAAAGAGCTGGAGATCACAAAGGAAAAGCTCCATACCATCGAACA AGCCTGGGAACAAGAAACGAAGTTAGGAAATGAATCAAACATGGATAAGGCAAAGAAATCAATGACAAAT AGTGACATTGTTTCTATTTCAAAAAAAATCACTGTGTTGGAGATGAAAGAATTAAATGAAAGGCAGAGGG CTGAACACTGTCAGAAAATGTATGAGCACCTAAGAACTTCATTGAAGCACATGGAAGAACGAAACTTTGA ATTGGAAACCAAGTTCACTGAGCTTACCAAAATCAACCTGGATGCACAGAAGGTGGAGCAGATGCTGAGA GATGAATTAGCTGATAGTGTGAACAAGGCAGTGAGCGACGCCGATAGACAGCGGATTCTAGAACTGGAGA AGCGTGAAGTGGAGCTCAAAGTGGAAGTGTCTAAACTGAGAGAGATTTCTGATATTGCCAAAAGACAAGT TGATTTTTTGAATTCACAACAACAGTCCAGGGAAAAGGAAGTGGAATCCCTCAGAATGCAGCTGCTGGAC TTCCAGGCACAATCTGATGAAAAGGCGCTAATTGCCAAATTGCACCAACACGTTGTCTCTCTTCAAATTA GTGAGGCCACAGCTCTTGGTAAGTTAGAGTCAGTTACGTCTAAACTCCAGAAGATGGAAGCCTGCAACTT GCGCTTAGAACAGAAACTGGACGAAAAGGAGCAGGCTCTCTACTACGCCCGCTTGGAGGGTAGAAACAGA GCCAATCACCTGCGCCAGACCATTCAGTCTCTACGACGACAGTTCAGTGGCGCTTTACCCTTGGCTCAGC AGGAAAAGTTCTCCAAAACAATGATTCAGTTGCAAAATGACAAACTTAAGATAATGCAAGAAATGAAAAA TTCTCAACAAGAACACAGAAATATGGAAAACAAAACATTGGAAATGGAATTAAAATTAAAAGGCTTAGAA GAATTAATAAGCACTTTAAAGGATGCCAGGGGAGCCCAGAAGGTAATCAATTGGCATGTGAAAATAGAAG AACTTCGCCTTCAAGAACTTAAGCTAAATAGAGAGCTAGTCAAGGGTAAAGAAGAAATCAAATATTTGAA TAATATCATCTCGGAATATGAACATACAATCAGCAGTCTGGAGGAAGACATTGTTCAGCAGAGCAAGTTT CATGAAGAAAGACAAATGGCTTGGGATCAAAGAGAAGTTGAGCTGGAACGCCAGTTAGACATTTTTGATC GTCAGCAAAATGAAATACTCAGTGCAGCACAAAAGTTTGAAGACGCGACAGGATCAATGCCTGACCCCAG CTTGCCCCTTCCAAACCAACTTGAAATTGCTCTAAGAAAAATTAAGGAGAATATTCAAATAATTCTTAAA ACACAAGCAACTTGCAAGTCACTAGAAGAGAAACTAAAAGAAAAAGAATCTGCTTTGCGGTTGGCAGAGC AAAATATTCTGTCAAGAGACAAAGTAATCAATGAACTGAGGCTTCAATTGCCTGCCACAGCTGATCGAGA AAAACTTATAGCTGAGCTCAACAGAACAGAGCTGGAGCCAAAATCTCATCACACGATGAAAATTGCCCAT CAAACCATTGCCAACATGCAGGCGAGGTTAAATCACAAGGAAGAAGTATTGAAGAAGTACCAGCATCTTT TGGAGAAAGCCAGAGAGGAGCAAAGAGAAATTGTTAAGAAACATGAGGAAGACCTTCATGTTCTTCATCA CAAATTAGAGCAACAGGCCGATAATTCACTCAGTAAATTCAAACAGACAGCTCAGGATTTAATTAAGCAG TCTCCTGCTCCAGTACCCACCAACAAGCATTTCATTCGCCTGGCCGAGATGGAACAGACAGTAGCAGAAC AAGATGACTCTCTCTCTTCACTTTTGACCAAACTGAAGAGAGTATCAAGAGATTTGGAAAGACAAAAAGA AGTCACTGAGTTCAAAGTCAGAGAATTTGAAACTACCAAATTACGGCTCCAAGAAACTCATGCCAACGAA GTAAAGAAAGTGAAAGCAGAGGTAGAGGACTTAAGGCATGCTCTAGCCCAAGCACAGAAGGACTCCCAGA ATTTAAAGTCTGAACTCCAGGCTCAGAAAGAAGCAAACTCCAGGGCACCAACAACCACAATGAGGAATCT AGTAGACAGGCTAAAGAGCCAGCTAGCTTTGAAAGAGAAGCAGCAAAAGGCACTTAGCCGAGCCCTGTTG GAACTTCGGTCAGAAATGACAGCAGCAGCTGAAGAACGTATAATTGCTGTAACTTCTCAAAAAGAGGCAA ATCTCAATGTTCAACAAGTTGTTGAGCGGCATACTAAAGAACTAAAGTCACAAATTGAAGATTTAAATGA AAATCTTTTGAAATTAAAAGAAGCTCTTAAGACAAGTAAAAACAAAGAAAATTCACTAGCTGATGACTTA AATGATTTAAATAATGAACTGCAAAAAAAGCAAAAAGCTTATAATAAAATCCTTAGAGAGAAAGATGGAA TTGATCAAGAAAATGATGAACTGAGACGACAGATTAAAAGACTGTCCAGTGGGCTACAGAGCAAAACTCT GATAGATAATAAGCAAAGTTTAATCGATGAACTTCAGAAGAAAGTTAAAAAACTTGAAAGCCAACTGGAA AGACAGGTGGATGACGTAGACATAAAGCCTATAAAGGAAAAGAGTGGTAAAGAAGAATTAATTAGGTGGG AAGAAGGTAAGAAATGGCAAACCAAAGTAGAGGGAATGCGCAACAAATTAAAGGAGAAAGAAGGAGAGGT CCACAGCCTGACAAAGCAGCTGAGCACCTTAAAGGAACTTTTTGCCAAAGCTGATAAAGAGAAGCTTACT TTGCAGAAGAAACTGAAAACAACAGGAATGACTGTTGACCAGGTCTTAGGAGTGCGAGCTTTGGAGTCGG AAAAGGAGTTGGAAGAGCTAAAAAGGAGAAATCTGGACTTAGAAAATGACATAGTATATATGAGGACCCA CCAGGCTCTTCCACGAGATTCTGTTGTAGAAGACTTACACTTACAAAATAGATACCTTCAAGAAAAACTT CATACTTTAGAAAATAAACTTTCCAAGGATGCATATTCTCAGTCTTTGACTTCAGAAATAGAATCAGATG ATCATTGTCAAAAAGAACAAGAGCTTCAGAGGGAAAATTTGAAATTGTCATCTGAAAATATTGAACTGAA ATTTCAACTGGAACAAGCAAAGAAAGACTTGCCGAGACTAAAGAATCAAGTAAGAGATTTGAAGGAAATG TGTGAAATTCTTAAGAAAGGGAAAATGGAACTTGAACGGAAGCTTGGTCAGGTCAGAGGGTCTGGTAGAA GTGGGAAGACAATCCCAGAGCTAGAAAAAACTATTGGGTTAATGAAGAAAGTTGTTGAAAAAGTCCAAAG AGAAAACGAACAATTGAAAAAGGCATCAGGACTACTGACTAGTGAAAAAATGGCTACTCTTGAGGAAGAA AATGGAAAATTAAAGGCTGAATTAGAAAAGCTGAAAGCTCACTTTGGACGTCAGTTGAGCATGCAGTTTG AATCTAAGAGCAAAGGGACTGAGAGAATTATGGCCGAAAATGAGCGGCTTCGGAAAGAACTTAAGAAAGA AATGCAAGCTTCTGAGAAACTGCGCATAGCTAAGAACAACTTAGAGCTGGTGAATGACAAGATGGCGGGT CAACTAGAAGAGACTGGGAAGAGATTGCAGTTTGCAGAAAGTAGAGGTCCACAGCTGGAAGGTGCTGACA GCCAGAGCTGGAAGTCAATTGTGGTTTCAAGAATATATGACACCAAGATAAAAGACCTCGAAAGTGACAT TGCCAAAAAGAACCAAAGTATCACCGACCTTAAACAGCTTGTAAGAGAAGCAACAGAGAGAGAACAGAAA GCCAAGAAGTACACTGAAGACCTTGAACAACAGATTGAGATCCTCAAAAATGTTCCTGAAGGAGCTGAAA CAGAGCAAGAGCTTATACAGCAGCTCCAGCTTCTTAGACTAGCCAATAACCAGCTGGATAAAGAAAAAGC AGAGCTAATCCATCAGATAGAAGTTATCAAGGACCAAACTGGAGCCGAAAGTAGCACACCTGATTCTGAT CAACTGAAGAAAAAGATAAATGACCTGGAGACACACCTCAGAAAGTCAGAGCCAGAAAAGCAACATTTGA AGGAGGAAATTAAAAAGCTGAAAAAAGAACTGGAAAATTTTGACCCTTCATTCTTTGAAGAAATTGAAGA CCTGAAGTACAATTATAAGGAAGAAGTGAAGAAAAATATTCTCTTAGAAGAGAAGTTAAACAAACTTTCT GAACAATTTGGATTTGAATTGCCTAGCCCACTTGCTGCTTCTGAACAACGGGAAGATGGAGAAAGTCCTA ATGGCGTCCCGATTTAT AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR215476 representing NM_001135755 Red=Cloning site Green=Tags(s) MPPNIKWKELIKVDPDELPRQEELADKLLISLSKVEVNELKNEDQENMIHLFRITQSLMKMKAQEVELAL EEVEKAGEEQAKFENQLKTKVMKLENELEMAQQSAGGRDTRFLRDEIRQLEKQLEQKDRELEDMEKELDK EKKVNEQLALRNEEAENENSKLRRENKRLKKKNEQLRQDIIDYQKQIDSQKESLLSRRGEDSDYRSQLSK KNYELVQYLDEIQTLTEANEKIEVQNQEMRKNLEESVQEMEKMTDEYNRMKAIVHQTDTVMDQIKKENEH YRLQVRELTDLLKAKDEEDDPVMMAVNAKVEEWKLILSSKDDEIIEYQQMLQSLRGKLKNAQLDADKSNI MALKQGIQERDSQIKMLTEQVEQYTKEMEKNTFIIEDLKNELRKDKGTSNIYQQTHYMKIHSKVQILEEK TKEAERTAELAEADAREKDKELVEALKRLKDYESGVYGLEDAVIEIKNYKAQIKIRDGEIEVLTKEINKL EMKINDVLDENEALRERAGLEPKTMIDLTEFRNSKRVKQQQYRAENQILLKEIESLEEERLDLKRKIRQM AQERGKRSAASGLTIDDLNLTETFSHGDKIGERKLNFISFNNMNETQSKNEFLSRELIEKEKDLERSRTV ITKFQNKLKELVEENKQLEEGMKEILQAIKDMPKDSDVKGSETSLIIPSLERLVNAMESKNAEGIFDASL HLKAQVDQLTGRNEELRQELRESRKEAVNYSQQLVKANLKIDHLEKETDLLRQSSGSNVVYKGIDLPDGI APSSAYIINSQNEYLIHLLQELDNKEKKLKHLEDSLEDYNRKFAVIRHQQSLLYKEYLSEKDVWKADSEM IKEEKRKLEDQAQQDAVKVKEYNNLLNALQMDSNEMKKMLSENSRKITVLQVNEKSLIRQYTTLVEIERQ LRKENGKHKNDVISMEAEVTEKIGNLQRFKEMATFRIAALQKVVDNSVSLSELELANKQYSELTAKYRDV LQKDNMLVQRASNLEHLACENASLKEQMEGLSKELEITKEKLHTIEQAWEQETKLGNESNMDKAKKSMTN SDIVSISKKITVLEMKELNERQRAEHCQKMYEHLRTSLKHMEERNFELETKFTELTKINLDAQKVEQMLR DELADSVNKAVSDADRQRILELEKREVELKVEVSKLREISDIAKRQVDFLNSQQQSREKEVESLRMQLLD FQAQSDEKALIAKLHQHVVSLQISEATALGKLESVTSKLQKMEACNLRLEQKLDEKEQALYYARLEGRNR ANHLRQTIQSLRRQFSGALPLAQQEKFSKTMIQLQNDKLKIMQEMKNSQQEHRNMENKTLEMELKLKGLE ELISTLKDARGAQKVINWHVKIEELRLQELKLNRELVKGKEEIKYLNNIISEYEHTISSLEEDIVQQSKF HEERQMAWDQREVELERQLDIFDRQQNEILSAAQKFEDATGSMPDPSLPLPNQLEIALRKIKENIQIILK TQATCKSLEEKLKEKESALRLAEQNILSRDKVINELRLQLPATADREKLIAELNRTELEPKSHHTMKIAH QTIANMQARLNHKEEVLKKYQHLLEKAREEQREIVKKHEEDLHVLHHKLEQQADNSLSKFKQTAQDLIKQ SPAPVPTNKHFIRLAEMEQTVAEQDDSLSSLLTKLKRVSRDLERQKEVTEFKVREFETTKLRLQETHANE VKKVKAEVEDLRHALAQAQKDSQNLKSELQAQKEANSRAPTTTMRNLVDRLKSQLALKEKQQKALSRALL ELRSEMTAAAEERIIAVTSQKEANLNVQQVVERHTKELKSQIEDLNENLLKLKEALKTSKNKENSLADDL NDLNNELQKKQKAYNKILREKDGIDQENDELRRQIKRLSSGLQSKTLIDNKQSLIDELQKKVKKLESQLE RQVDDVDIKPIKEKSGKEELIRWEEGKKWQTKVEGMRNKLKEKEGEVHSLTKQLSTLKELFAKADKEKLT LQKKLKTTGMTVDQVLGVRALESEKELEELKRRNLDLENDIVYMRTHQALPRDSVVEDLHLQNRYLQEKL HTLENKLSKDAYSQSLTSEIESDDHCQKEQELQRENLKLSSENIELKFQLEQAKKDLPRLKNQVRDLKEM CEILKKGKMELERKLGQVRGSGRSGKTIPELEKTIGLMKKVVEKVQRENEQLKKASGLLTSEKMATLEEE NGKLKAELEKLKAHFGRQLSMQFESKSKGTERIMAENERLRKELKKEMQASEKLRIAKNNLELVNDKMAG QLEETGKRLQFAESRGPQLEGADSQSWKSIVVSRIYDTKIKDLESDIAKKNQSITDLKQLVREATEREQK AKKYTEDLEQQIEILKNVPEGAETEQELIQQLQLLRLANNQLDKEKAELIHQIEVIKDQTGAESSTPDSD QLKKKINDLETHLRKSEPEKQHLKEEIKKLKKELENFDPSFFEEIEDLKYNYKEEVKKNILLEEKLNKLS EQFGFELPSPLAASEQREDGESPNGVPIY SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001135755
ORF Size	7437 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001135755.1, NP_001129227.1
RefSeq Size	8251 bp
RefSeq ORF	7440 bp
Locus ID	314787
MW	289.7 kDa

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