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Chrm3 (NM_012527) Rat Tagged ORF Clone
CAT＃: RR215296
- TrueORF®
Chrm3 (Myc-DDK-tagged ORF) - Rat cholinergic receptor, muscarinic 3 (Chrm3), (10 ug)
"NM_012527" in other vectors (3)
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CNY 7,510.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR215296 representing NM_012527
Red=Cloning site Blue=ORF Green=Tags(s) CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC GCCC ATGACCTTGCACAGTAACAGTACAACCTCGCCTTTGTTTCCCAACATCAGCTCTTCCTGGGTGCACAGTC CCTCGGAGGCAGGGCTGCCCTTGGGGACAGTCACTCAGTTGGGCAGCTACAACATTTCACAAGAAACTGG GAATTTCTCCTCAAACGACACCTCCAGCGACCCTCTCGGGGGTCACACCATCTGGCAAGTGGTCTTCATT GCCTTCTTAACCGGCTTCCTGGCATTGGTGACCATCATTGGCAACATCCTTGTCATTGTGGCCTTCAAGG TCAACAAACAGCTGAAGACAGTCAACAACTACTTCCTCTTAAGCCTGGCCTGTGCAGACCTGATCATCGG GGTCATTTCCATGAACCTGTTCACTACCTACATCATTATGAACCGTTGGGCACTGGGGAACTTAGCCTGC GACCTCTGGCTTTCCATTGACTATGTGGCCAGCAATGCCTCTGTCATGAATCTGCTGGTCATCAGCTTTG ACAGGTACTTTTCCATCACTAGACCACTCACCTACCGAGCCAAAAGAACAACAAAACGAGCTGGTGTGAT GATTGGTCTGGCTTGGGTCATCTCCTTTGTCCTATGGGCTCCTGCCATCTTGTTCTGGCAATACTTTGTA GGGAAGAGAACTGTGCCCCCAGGAGAATGTTTCATTCAGTTTCTGAGTGAGCCCACCATCACCTTCGGCA CGGCGATCGCTGCCTTTTACATGCCTGTCACCATCATGACTATTTTATACTGGAGGATCTATAAGGAAAC TGAGAAGCGTACCAAAGAGCTGGCTGGCCTACAGGCCTCTGGGACAGAAGCGGAGGCAGAAAACTTTGTC CACCCCACAGGCAGTTCTCGAAGCTGTAGCAGCTATGAACTGCAACAGCAAGGCGTGAAACGATCATCCA GGAGGAAGTACGGTCGCTGTCACTTCTGGTTCACCACCAAGAGCTGGAAGCCCAGTGCCGAGCAGATGGA CCAAGACCACAGCAGCAGCGACAGTTGGAACAACAACGATGCTGCTGCCTCCCTGGAAAACTCTGCTTCC TCCGATGAAGAGGACATTGGCTCAGAGACCAGGGCCATCTATTCCATTGTCCTCAAGCTTCCAGGCCATA GCTCCATCCTCAACTCTACCAAGCTACCGTCCTCAGATAACCTGCAGGTGTCCAACGAGGACCTGGGGAC TGTGGATGTGGAGAGAAATGCTCACAAGCTTCAGGCCCAGAAGAGCATGGGTGATGGTGACAACTGTCAG AAGGATTTCACCAAGCTTCCCATCCAGTTAGAGTCTGCCGTGGACACAGGCAAGACCTCTGACACCAACT CCTCGGCAGACAAGACCACGGCTACTCTACCTCTGTCCTTCAAGGAGGCCACGCTGGCTAAGAGGTTTGC TCTCAAGACCAGAAGTCAGATCACCAAGCGGAAGAGGATGTCGCTCATCAAGGAGAAGAAGGCCGCCCAG ACGCTCAGTGCCATCTTGCTAGCCTTCATCATCACGTGGACCCCCTACAACATCATGGTCCTGGTGAACA CCTTCTGTGACAGCTGCATACCCAAAACCTATTGGAATCTGGGCTACTGGCTGTGCTATATCAACAGCAC CGTGAACCCTGTGTGCTATGCCCTGTGCAACAAAACATTCAGAACCACCTTCAAGACGCTCCTCTTGTGC CAGTGTGACAAAAGGAAGAGGCGCAAACAGCAGTACCAGCAGAGACAGTCGGTCATTTTTCACAAGCGAG TGCCGGAACAGGCCTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR215296 representing NM_012527
Red=Cloning site Green=Tags(s) MTLHSNSTTSPLFPNISSSWVHSPSEAGLPLGTVTQLGSYNISQETGNFSSNDTSSDPLGGHTIWQVVFI AFLTGFLALVTIIGNILVIVAFKVNKQLKTVNNYFLLSLACADLIIGVISMNLFTTYIIMNRWALGNLAC DLWLSIDYVASNASVMNLLVISFDRYFSITRPLTYRAKRTTKRAGVMIGLAWVISFVLWAPAILFWQYFV GKRTVPPGECFIQFLSEPTITFGTAIAAFYMPVTIMTILYWRIYKETEKRTKELAGLQASGTEAEAENFV HPTGSSRSCSSYELQQQGVKRSSRRKYGRCHFWFTTKSWKPSAEQMDQDHSSSDSWNNNDAAASLENSAS SDEEDIGSETRAIYSIVLKLPGHSSILNSTKLPSSDNLQVSNEDLGTVDVERNAHKLQAQKSMGDGDNCQ KDFTKLPIQLESAVDTGKTSDTNSSADKTTATLPLSFKEATLAKRFALKTRSQITKRKRMSLIKEKKAAQ TLSAILLAFIITWTPYNIMVLVNTFCDSCIPKTYWNLGYWLCYINSTVNPVCYALCNKTFRTTFKTLLLC QCDKRKRRKQQYQQRQSVIFHKRVPEQAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
AscI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012527 |
| ORF Size | 1767 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012527.2, NP_036659.2 |
| RefSeq Size | 4086 bp |
| RefSeq ORF | 1770 bp |
| Locus ID | 24260 |
| UniProt ID | P08483 |
| MW | 66.1 kDa |
| Gene Summary | G-protein coupled receptor, muscarinic receeptor subtype that mediates cholinergic-induced contraction in most smooth muscle [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN215296 | Chrm3 (untagged ORF) - Rat cholinergic receptor, muscarinic 3 (Chrm3), (10 ug) |
CNY 7,024.00 |
|
| RR215296L3 | Lenti ORF clone of Chrm3 (Myc-DDK-tagged ORF) - Rat cholinergic receptor, muscarinic 3 (Chrm3), (10 ug) |
CNY 9,410.00 |
|
| RR215296L4 | Lenti ORF clone of Chrm3 (mGFP-tagged ORF) - Rat cholinergic receptor, muscarinic 3 (Chrm3), (10 ug) |
CNY 10,360.00 |
