Med13 (NM_001107035) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Thrap1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR215174 representing NM_001107035 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACCGAAACTTTGTACGAATTGGCAAGTGGTTTGTGAAGCCATATGAGAAAGATGAAAAACCTATAA ATAAAAGTGAACACTTGTCCTGCTCTTTCACCTTTTTCTTGCATGGAGACAGCAATGTTTGTACCAGTGT GGAAATTAACCAACATCAACCTGTGTACCTTCTCAGTGAAGAGCATGTCACCCTTGCTCAGCAGTCTAAT AGCCCATTTCAAGTTATCTTAAGTCCATTTGGACTAAATGGCACTCTCACGGGACAGGCTTTCAAGATGT CTGATTCAGCTACGAAAAAATTAATTGGTGAATGGAAACAGTTCTATCCTATTTCATGTGGTTTGAAGGA GATGTCTGAAGAAAAACAGGAAGACATGGATTGGGAAGATGATTCTTTAGCTGCAGTAGAAGTTCTTGTT GCGGGTGTCCGAATGATCTACCCAGCATGTTTTGTTCTAGTCCCTCAATCAGACATTCCTACCCCTAGTT CTGTGGGATCCTCTCACTGTTCAGCTTCCTGCTTGGGTATCCATCAAGTGCCTGCTTCCACAAGAGATCC TGCTATGTCTTCAGTTACTCTCACTCCACCCACATCTCCTGAGGAAGTCCAATCAGTTGATCCTCAGTCT GCACAGAAGTGGGTCAAATTTTCTTCAGTATCCGATGGCTTCAGCACTGACAGTACTAGCCATCATGGTG GGAAAATACCAAGAAAATTAGCAAATCATGTGGTGGATCGAGTCTGGCAAGAATGTAATATGAACAGATT ACAGAACAAGAGGAAGTATTCTGCTACATCCAGTGGCCTGTGTGAAGAAGAGACAGCTGATAAAATAGGA AGCTGGGACTTTGTTGAAGCCACACAAAGAACAAGCTGTAGTTGTTTGAGGCATAAAAGTCTTAAAACAA GAAATACTGGGCAACAAGGACAGGCACCATCTTTAGGGCAACAACAGCAAATACTTCCTAAGCACAAGAC CAATGAGAAGCAAGAAAAGAGTGAGAAGCCACAAAAGCGCCCCTTGACCCCTTTTCACCACCGTGTATCA ATTAGTGATGATATTGGCATGGACACAGATTCAGCCAGCCAAAGACTTGTGATCTCTGCTGCAGATAGTC AAGTGAGATTTACAAACATCCGAACTAATGATGTAGCAAAGACTCCTCAGATGCATGGCACTGAACTGGC AAATTCACCTCAGCCTCCCCCACTTAGTCCTCATCCATGTGATGTGGTTGATGAAGGAGTGACTAAAACG CCTTCAACTCCCCAGAGTCAACATTTTTATCAAATGCCAACACCGGATCCCTTGGTTCCTACCAAACCAA TGGAAGATAGGATAGACAGTTTGTCCCAGTCTTTCCCACCTCCATTTCAGGAAGCTGTAGAACCTACCGT GTATGTTGGTACAGCAGTAAGCTTGGAAGAAGATGAAGCTAATGTAGCCTGGAAGTATTACAAAGTCCCA AAGAAAAAAGATGTAGAATTTTTACCACCTCAACTTCCAAATGATAAATTCAAGGATGATCCAGTTGGAC CTTTTGGACAGGAAAGTGTAACATCAGTTACAGAGTTAATGGTGCAGTGTAAGAAGCCTTTAAAAGTTTC TGATGAAATAGTCCAGCAATATCAAATTAAAAATCAGTATCTTTCAGCAATAGCATCTGATACAGAACAA GAACCTAAAATTGATCCATATGCGTTTGTTGAAGGAGATGAGGAATTCATTTTTACTGATAAAAAAGATA GACAAAATAGCGAGAGAGAAGCTGGGAAGAAACACAAGGTGGAAGATGGGACATCTGCTGTCACAGTGTT ATCTCATGAAGAAGATGCTATGTCATTATTTAGTCCCTCTAAGCAAGATGCTCCACGCCCTGCTAATCAT GCCCGTCCTCCATCAACCAGTTTGATTTATGACTCAGATCTGGCTGTGTCTTATACTGACCTTGATAACC TCTTCAATTCTGATGAAGATGAATTAACACCTGGATCTAAAAAATCAGCAAGTGGATCAGATGATAAAGC CAGCTCCAAAGAATCAAAGACAGGAAGCCTGGATCCGTTGTCTTGCATAAGCACTGCAGACCTTCACAAA ATGTACCCCACGCCTCCTTCACTGGAGCAGCACATTATGGGCTTCTCTCCAATGAACATGAATAACAAGG AATACGGTAGTATGGACCCCACACCTGGAGGAACTGTGCTGGAAGGAAGTAGCTCTAGTGTAGGAACACA GTTCAGAATTGAGGTTGAGGAAGGGTTCTGTAGCCCTAAACCTTCTGAAATTAAAGATTTTTCTTACGTC TATAAGCCTGAAAATTGTCAAGTTCTAGTAGGTTGTTCCATGTTTGCACCTTTAAAAACTCTACCAAGCC ATTGTCTGCCTCCTATCAAATTGCCGGAAGAGTGTGTTTACCGGCAGAGCTGGACTGTTGGGAAATTGGA CTTGCTTCCTTCAGGCCCTTCAATGCCATTCATCAAAGAGGGTGATGGCAGTAATTTGGATCAAGACTAT GGTCCTGCCTATACACCACAAATCCATACATCTTTTGGGATGCCTCCGAGCAGTGCACCTCCTAGTAATG GTGGCGCAGGAATTCTTCCTTCCCCATCCACCCCTAGGTTTCCGACTCCAAGAACTCCAAGGACTCCAAG GACTCCTCGTGGGGCTGGTGGACCTGCTAGTGCTCAAGGTTCAGTCAAGTATGAGAATTCAGACTTGTAT TCACCAGCTTCCACACCATCTACATGCAGACCCCTCAATTCTGTTGAACCTGCAACTGTCCCTTCCATCC CTGAAGCGCACAGTCTTTATGTAAACCTCATCCTTTCAGAATCAGTAATGAACTTGTTTAAAGACTGTAA TTTTGATAGTTGCTGTATTTGTGTCTGCAACATGAATATCAAGGGTGCCGATGTTGGAGTTTACATCCCA GACCCAACGCAGGAAGCACAGTACCGGTGTACCTGTGGCTTCAGTGCTGTCATGAACCGAAAGTTTGGAA ATAATTCAGGATTATTTCTTGAAGATGAACTTGACATCATAGGACGTAACACAGACTGTGGCAAAGAAGC AGAAAAACGTTTTGAAGCTCTCAGGGCTTCCTCTGTGGAAAATGTTAATGGAAGCCTAAAGGAATCTGAA AAGGTGCCTGATGAGTTGATATTACTGCTACAAGATCAATGCACTAATTTATTTTCACCCTTTGGAGCAG CAGACCATGATCCCTTTCCCAAAGCGGGTATAAGTAGCAATTGGGTGCGTGTTGAGGAGCGGGACTGTTG TAGTGACTGTTGTCTCGCATTGGAACATGGGCGTCAGTTCATGGATAATATGTCGGGAGGAAAAGTTGAT GAAGCACTTGTCAGAAGCTCATGCTTACACCCATGGGCCAAGCAAAATGATGCGAGTGTGCAGTGTTCAC AGGATATACTTCGGATGCTCCTTTCTCTTCAGCCAGTTCTTCAAGATGCTATTCAGAAAAAAAGAACAGT GAGGCCTTGGGGGGTTCAAGGTCCTCTCACATGGCAACAATTTCATAAAATGGCTGGCCGAGGCTCTTAT GGAACTGATGAATCTCCAGAACCACTGCCGATTCCCACGTTTTTGTTGGGTTATGATTATGATTTTCTGG TGCTTTCTCCATTTGCTCTCCCTTACTGGGAGAAACTTATGCTGGAACCTTATGGATCTCAAAGAGATAT AGCCTATGTTGTACTCTGTCCAGAGAATGAAGCTTTGTTAAATGGAGCCAGAAGCTTTTTTAGAGATCTC ACAGCAATATACGAGTCCTGTCGATTGGGCCAGCACAGACCTATTTCTCGACTGTTGACTGATGGCATCA TGAAAGTGGGAGCTACTGCATCGAAGAAACTTTCGGAGAAGTTTGTAACAGAATGGTTTTCTCAGGCAGC TGATGGTAACAATGAAGCCTTTTCTAAATTGAAGCTGTATGCACAAGTCTGCAGATACGATCTAGGTCCA TATCTTGCTTCTCAGCCGCTGGACAGTTCTCTGCTCTCCCAGCCAAATTTAGTTGCCCCTCCAAATCAGT CTTTGGTTACTGCACCTCAGATGACAAATACTGGAAACGCTAATGCTCCATCTGCCACCTTAGCATCTGC GGCAAGTAGCACTATGACACTGACTCCAGGTGTTGCCATATCTACTTCAGTTGCCACAGCTAATTCAACT TTGACCACAACTTCATCTTCATCATCTTCCAGTATGAGTAGTGGAGTATCATCAAATAAACTGCCTTCGT TTCCACCCTTTGGCAGTATGAACACTAGTGGTACAGGATCCATGTCTGCACAAGGAAGTACGGTTCAGAG TGGCCAGCTAGGAGGACAGCAGTCATCATCTCTGCAGGCAGCAGGAATATCGGGGGAGTCGGCTTCTCTG CCCACTCAGCCACATCCTGACGTGTCTGAAAGCACAATGGATCGGGATAAAGTGGGGATTCCCACAGATG GTGATTCACATGCAATTACCTACCCACCTGCAATTGTTGTTTATATAATTGATCCTTTTACATACGAGAA TAAAGATGAGAGCACCAGCTCTTCTAATGTATGGACTTTGGGGCTCCTTCGATGCTTCTTGGAAATGCTC CAGACTCTTCCTCCTCATATCAAAAGTACTGTTTCTGTACAGATTGTTCCTTGTCAGTACCTGTTACAAC CTGTGAAACATGACGACAGACAAATCTACTCCCAGCATTTAAAATCTTTGGCTTTTTCGGCATTTACCCA GTGTCGGCGACCACTTCCAACATCAACCAATGTCAAAACACTGACTGGGTTTGGTCCAGGCTTAGCTATG GAAACTGCTCTTAAAAGTCCTGATAGACCAGAGTGTATTCGACTTTATACGCCGCCTTTTATTCTGGCTC CTGTGAAGGACAAACAGACAGAGCTAGGAGAAACCTTTGGCGAAGCTGGACAGAAATACAATGTTCTCTT TGTGGGCTACTGTTTATCACATGATCAAAGATGGATTCTTGCATCTTGCACAGATCTATATGGAGAACTT CTAGAAACATGTATCATTAACATCGATGTTCCAAATAGGGCTCGTCGGAAAAAGGGTTCTGCTAGAAGAT TTGGTCTACAGAAACTTTGGGAGTGGTGCTTAGGACTTGTTCAGATGAGCTCTTTACCATGGAGAGTTGT AATTGGTCGCCTGGGTAGGATTGGACATGGAGAATTGAAAGACTGGAGTTGTTTGCTGAGTCGTCGAAAC TTGCAGTCTCTAAGCAAAAGGCTCAAAGACATGTGTCGGATGTGTGGTATATCTGCTGCAGACTCTCCGA GTATCCTTAGTGCTTGTTTGGTTGCAATGGAACCTCAGGGTTCTTTTGTTATCATGCCAGATTCTGTGTC AACTGGTTCTGTTTTTGGAAGAAGCACCACTCTAAATATGCAGACGCCCCAGCTAAACACCCCACAAGAT ACATCCTGTACTCATATACTTGTGTTTCCTACTTCTGCTTCTGTGCAAGTAGCCTCAGCTACTTACACCA CTGAAAATTTGGATTTAGCTTTCAATCCCAACAACGATGGAGCAGATGGAATGGGTATCTTTGATTTATT AGACACAGGAGATGATCTTGATCCTGATATCATTAATATCCTTCCTGCATCTCCAACTACATCTCCTGTA CATTCTCCAGGCTCTCATTACCCCCATGGAGGTGATGCTGGCAAGGGTCAGGGTACTGATCGGCTACTTT CAACAGAATCGCACGATGAAGTAACTAATATTCTTCAACAACCATTGGCCCTTGGATACTTTGTATCAAC TGCCAAAGCGGGTCCATTACCTGACTGGTTCTGGTCAGCATGTCCTCAAGCACAATATCAATGCCCCCTT TTTCTTAAGGCCTCTTTGCACCTCCACGTGCCTTCAGTGCAATCTGACGAACTGCTTCACAGTAAACACT CCCACCCACTTGACTCAAATCAGACTTCAGATGTCCTCAGGTTTGTTTTGGAACAGTACAATGCACTCTC GTGGCTAACCTGTGACCCTGCAATCCAGGACAGACGCTCATGTCTCCCAATTCATTTTGTGGTGCTGAAT CAGTTATATAACTTTATCATGAATATGCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR215174 representing NM_001107035 Red=Cloning site Green=Tags(s) MNRNFVRIGKWFVKPYEKDEKPINKSEHLSCSFTFFLHGDSNVCTSVEINQHQPVYLLSEEHVTLAQQSN SPFQVILSPFGLNGTLTGQAFKMSDSATKKLIGEWKQFYPISCGLKEMSEEKQEDMDWEDDSLAAVEVLV AGVRMIYPACFVLVPQSDIPTPSSVGSSHCSASCLGIHQVPASTRDPAMSSVTLTPPTSPEEVQSVDPQS AQKWVKFSSVSDGFSTDSTSHHGGKIPRKLANHVVDRVWQECNMNRLQNKRKYSATSSGLCEEETADKIG SWDFVEATQRTSCSCLRHKSLKTRNTGQQGQAPSLGQQQQILPKHKTNEKQEKSEKPQKRPLTPFHHRVS ISDDIGMDTDSASQRLVISAADSQVRFTNIRTNDVAKTPQMHGTELANSPQPPPLSPHPCDVVDEGVTKT PSTPQSQHFYQMPTPDPLVPTKPMEDRIDSLSQSFPPPFQEAVEPTVYVGTAVSLEEDEANVAWKYYKVP KKKDVEFLPPQLPNDKFKDDPVGPFGQESVTSVTELMVQCKKPLKVSDEIVQQYQIKNQYLSAIASDTEQ EPKIDPYAFVEGDEEFIFTDKKDRQNSEREAGKKHKVEDGTSAVTVLSHEEDAMSLFSPSKQDAPRPANH ARPPSTSLIYDSDLAVSYTDLDNLFNSDEDELTPGSKKSASGSDDKASSKESKTGSLDPLSCISTADLHK MYPTPPSLEQHIMGFSPMNMNNKEYGSMDPTPGGTVLEGSSSSVGTQFRIEVEEGFCSPKPSEIKDFSYV YKPENCQVLVGCSMFAPLKTLPSHCLPPIKLPEECVYRQSWTVGKLDLLPSGPSMPFIKEGDGSNLDQDY GPAYTPQIHTSFGMPPSSAPPSNGGAGILPSPSTPRFPTPRTPRTPRTPRGAGGPASAQGSVKYENSDLY SPASTPSTCRPLNSVEPATVPSIPEAHSLYVNLILSESVMNLFKDCNFDSCCICVCNMNIKGADVGVYIP DPTQEAQYRCTCGFSAVMNRKFGNNSGLFLEDELDIIGRNTDCGKEAEKRFEALRASSVENVNGSLKESE KVPDELILLLQDQCTNLFSPFGAADHDPFPKAGISSNWVRVEERDCCSDCCLALEHGRQFMDNMSGGKVD EALVRSSCLHPWAKQNDASVQCSQDILRMLLSLQPVLQDAIQKKRTVRPWGVQGPLTWQQFHKMAGRGSY GTDESPEPLPIPTFLLGYDYDFLVLSPFALPYWEKLMLEPYGSQRDIAYVVLCPENEALLNGARSFFRDL TAIYESCRLGQHRPISRLLTDGIMKVGATASKKLSEKFVTEWFSQAADGNNEAFSKLKLYAQVCRYDLGP YLASQPLDSSLLSQPNLVAPPNQSLVTAPQMTNTGNANAPSATLASAASSTMTLTPGVAISTSVATANST LTTTSSSSSSSMSSGVSSNKLPSFPPFGSMNTSGTGSMSAQGSTVQSGQLGGQQSSSLQAAGISGESASL PTQPHPDVSESTMDRDKVGIPTDGDSHAITYPPAIVVYIIDPFTYENKDESTSSSNVWTLGLLRCFLEML QTLPPHIKSTVSVQIVPCQYLLQPVKHDDRQIYSQHLKSLAFSAFTQCRRPLPTSTNVKTLTGFGPGLAM ETALKSPDRPECIRLYTPPFILAPVKDKQTELGETFGEAGQKYNVLFVGYCLSHDQRWILASCTDLYGEL LETCIINIDVPNRARRKKGSARRFGLQKLWEWCLGLVQMSSLPWRVVIGRLGRIGHGELKDWSCLLSRRN LQSLSKRLKDMCRMCGISAADSPSILSACLVAMEPQGSFVIMPDSVSTGSVFGRSTTLNMQTPQLNTPQD TSCTHILVFPTSASVQVASATYTTENLDLAFNPNNDGADGMGIFDLLDTGDDLDPDIINILPASPTTSPV HSPGSHYPHGGDAGKGQGTDRLLSTESHDEVTNILQQPLALGYFVSTAKAGPLPDWFWSACPQAQYQCPL FLKASLHLHVPSVQSDELLHSKHSHPLDSNQTSDVLRFVLEQYNALSWLTCDPAIQDRRSCLPIHFVVLN QLYNFIMNML TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001107035
ORF Size	6120 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107035.1, NP_001100505.1
RefSeq Size	7024 bp
RefSeq ORF	6123 bp
Locus ID	303403
MW	223.5 kDa

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