Apc2 (NM_001106769) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR215150 representing NM_001106769 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGCTCCGTGGCCTCGTACGAGCAGCTGGTGCGCCAGGTGGAGGCCTTGAAGGCGGAGAACACTC ACTTAAGACAGGAGCTGAGGGATAACTCTAGCCATCTCTCCAAGCTGGAGACAGAGACTTCTGGCATGAA GGAGGTCCTGAAACACCTTCAGGGCAAGCTGGAGCAGGAGGCGAGAGTGCTGGTATCCTCCGGGCAGACA GAAGTGTTAGAGCAACTGAAAGCTCTTCAGACTGACATCAGTAGCCTCTATAACCTGAAGTTCCATGCCC CGGCCCTGGGCCCTGAGCCTGCTGCCCAGACTCCAGAGGGAAGCCCAGTACATGGCCCTGCACCGTCCAA GGACAGCTTCGGAGAGCTGAGCCGGGCCACCATCCGCCTGCTGGAAGAACTAGACCAGGAGAGATGCTTC CTGCTGAGCGAGATAGAGAAAGAGGAGAAGGAGAAGCTGTGGTATTACTCTCAGCTCCAGGGCCTCTCTA AGCGCTTGGATGAACTGCCACACGTAGACACGCAGTTTTCGATGCAGATGGATCTAATTCGGCAGCAGCT GGAGTTCGAAGCCCAGCACATCCGCTCGTTGATGGAGGAGCGCTTCGGTACCTCAGACGAGATGGTGCAG CGGGCTCAGATCCGGGCTTCACGCCTGGAGCAGATTGACAAGGAGCTGTTGGAAGCCCAGGACCGGGTGC AGCAGACGGAGCCCCAGGCGCTGCTGGCGGTGAAGCCTGTGGCAGTGGAGGAGCAGGAGGCAGAAGTTCC CACACACCCTGAGGATGTCACCCCCCAGCCTGGCAACAGCAAGGTGGAGGTCGTGTTCTGGCTTCTATCT ATGTTGGCAACGCGCGACCAGGAAGATACTGCGCGCACGCTGCTCGCCATGTCCAGCTCGCCAGAGAGCT GCGTAGCCATGCGCCGCTCGGGATGTCTGCCACTGCTGCTCCAGATCCTTCATGGCACTGAGGCTGGGTC TGTGGGGCGCACAGGAACCCCCGGAGCGCCTGGTGCCAAAGATGCACGCATGCGTGCCAACGCGGCCCTG CACAACATAGTCTTCTCCCAGCCGGATCAGGGCCTGGCACGCAAGGAGATGCGTGTGCTGCACGTGCTGG AGCAGATCCGAGCCTACTGCGAGACCTGCTGGGACTGGCTTCAGGCGCGGGACAGCGGGACAGAGAGTGG TACAGGAGACACTCCTGTCCCTATCGAGCCTCAGATCTGCCAGGCCGCCTGTGCAGTGATGAAGCTGTCA TTTGACGAGGAATACCGTCGGGCTATGAATGAGCTAGGGGGGCTGCAGGCTGTGGCCGAGCTACTGCAGG TGGATTATGAGATGCACAAGATGACCCAGGATCCACTCAACCTCGCCCTGCGCCGCTACGCTGGCATGAC CCTCACCAACCTCACCTTCGGAGACGTTGCCAACAAGGCCACTCTGTGTGCCCGCCGAGGCTGCATGGAA GCCATTGTGGCCCAGCTGGCCTCTGAGAGCGAGGAGCTGCATCAGGTTGTTTCCAGTATTCTGCGTAACC TGTCGTGGAGGGCGGACATCAACAGCAAGAAGGTGCTGAGGGAGGTTGGCAGCATGACCGCTTTGATGGA ATGTGTGCTGCGGGCCTCCAAGGAGTCCACTCTAAAGAGCGTGCTCAGTGCTCTGTGGAACCTGTCGGCA CACAGCACAGAGAACAAGGCAGCTATCTGCCAGGTGGATGGCGCGCTGGGTTTCCTGGTGAGCACCCTAA CATACCGTTGCCAAGGGAACTCCCTAGCAGTCATCGAGAGTGGCGGTGGGATCCTGCGCAACGTGTCAAG CCTCATTGCCACGCGGGAGGACTACAGGCAGGTGCTCCGTGACCACAACTGCCTACAGACACTGCTGCAG CACCTCACGTCGCACAGCTTGACTATCGTGAGCAATGCCTGCGGCACCCTTTGGAACCTGTCTGCCCGCA GCCCCCGCGACCAGGAACTGTTGTGGGACCTGGGGGCTGTGGGCATGCTACGAAACCTCGTCCACTCCAA ACACAAGATGATCGCCATGGGTAGCGCTGCCGCTCTGCGGAACCTGCTGGCCCACCGACCGGCCAAGTAT CAGGCCGCAGCCATGGCAGTCTCCCCAGGCACCTGCGTGCCCAGTCTGTACGTCCGCAAGCAGAGGGCCC TGGAGGCCGAGCTGGACACTCGGCACCTGGTGCATGCGCTCGGTCACTTGGAGAAGCAGGGTCTGCCTGA GGCAGAGACCACTTCAAAGAAGCCCCTGCCACCCCTCCGCCACCTGGACGGGCTGGTGCAGGACTATGCC TCTGATTCTGGCTGCTTTGATGATGATGATGCACCATCCCTGGCTGCTGCGGCCACCACAGCTGAGCCCG CCAGCCCAGCAGTGATGTCTATGTTCCTTGGTGGTCCCTTCCTTCAGGGCCAGGCACTGGCCCGCACCCC ACCTGCCCGCCAGGGTGGCCTAGAAGCTGAGAAGGAGACTGGTGGGGAGGCAGCTGTGGCTGCTAAAGCC AAGGCCAAGCTAGCGTTGGCTGTGGCTCGCATCGACCGACTGGTGGAGGACATCTCTGCCCTGCACACCT CATCAGACGACAGCTTCAGTCTCAGCTCGGGGGACCCGGGGCAGGAGGCGCCAAGGGAGGGCCGTGCGCA GTCCTGCTCTCCATGCCGGGGCACAGAGGGTGGGCGGCGTGAGGCTGGCAGCCGGGCGCACCCTCTGCTG AGGCTCAAGGCGGCCCACACCAGCCTCTCCAACGACAGCCTGAACAGTGGTAGCACCAGCGATGGCTACT GTACCCGGGAACACATGACGCCTTGCCCACTGGCCGTGTTGACCGAGCACCGCGATGGCCCAGTATGCGG ACAGACTCGGCCCAGCCGACTGGACCTAGACCTTCCCAGCCGGGCTGAGCTTCCTGCCCGGGACACAGCA GCCACTGATGCCCGAGTGCGCACCATTAAGTTATCCCCAACCTATCAGCACGTGCCACTGCTTGATGGGG CCCCGGGGGCAGGTGTCAGACCCCTGGTTGGGCTTGGAACCTCCCCGGGGACTCGGAAACAGGCATGGAT ACCTGCAGATAGTTTGAGTAAAGTCCCCGAGAAGCTGGTGGCCTCCCCACTGCCGGTGACTAGCAAGGTG CTGCAGAAGCTGGTGGCACAGGATGGGCCGATGTCCCTCTCCAGGTGCAGTTCCCTGTCTTCTCTGTCTT CCACGGGCCATGCAGTCCCCAGCCAGGTGGAGAACCTTGATGACAGCGATTCATCCCTGGAAGAGCTTGA GGAGGCTGGTCCTGGCGAGGCCGAGCTAGACAGGGCGTGGAGAGCATCCGGGTCCACCTCTCTTCCGGTG TCCATCCCGGCCCCACAGCGGGGTCGAAGTCGAGGTCTTGGGGTGGAGGATGCTACACCATCCAGTTCAT CTGAGAACTGCGTCCAGGAGACACCCTTGGTCCTGAGCCGCTGTAGCTCTGTGAGCTCCCTGGGCAGCTT CGAGAGCCGTTCCATCGCCAGCTCCATCCCGAGCGACCCCTGCAGTGCGCTGGGCAGTGGTACAGTGAGT CCCAGCGAGCTGCCAGACAGCCCCGGCCAGACAATGCCACCCAGCCGCAGCAGGACTCCACCGGCACCTC CCGGGCAGCCCGAGGCCAGCCAGTTCAGCCTGCAGTGGGAAAGTTACGTGAAGCGCTTCCTGGACATCGC AGACTGTCGGGAAAGATGCCAGCCGCCCTCGGAGCTGGACGCGGGCAGCGTACGCTTCACGGTGGAGAAG CCGGACGAGAATTTCTCTTGTGCTTCCAGCCTCAGCGCACTGGCCCTGCATGAGCTGTATGTCCAGCAGG ACGTGGAACTGCGGCTGAGGCCACCAGCCTGTCCAGAACGTGCGGTGGGTGGTGGTGGGGGCCACCGTCG GAGGGACGAGGCTACCAGCCGCCTGGATGGCCCAGCACCAGCTGGCTCTAGGGCTCGGTCAGCGGCTGAT AAAGAACTAGAGGCGCTGCGTGAATGTCTGGGGGCAGCCATGCCGGCCCGGCTCCGCAAGGTGGCCTCGG CCTTGGTGCCTGGCCGCCGTGCACTGCCGGTCCCCGTGTACATGTTAGTGCCCGCCCCGGCTCGGGGTGA TGACTCGGGCACAGACTCTGCAGAGGGCACGCCTGTCAACTTTTCCAGTGCAGCCTCTCTCAGTGACGAG ACCTTACAGGGACCCTCCAGGGATAAGCCGGCCAGGCCTGGGGACAGGCAGAAACCTACAGGCCGAGCTG CTCCTGCCAGGCAGACCCGTGGGCACCGGCCCAAGGCAGCAGGAGCAGGTGCTGGTAAGAGCACAGAGCA CACACGGGGGGCTAATAGGAACCGGGCAGGATTGGAACTACCCCTCAGCCGGCCCCAGAGTGCTCGGTCC AACAGGGATGGCTCATGCCAGACCCGGACCCGTGGAGACGGTGCCCTGCAGTCGCTATGCCTCACGACAC CCACAGAGGAAGCTGTGTACTGCTTCTATGACTCTGACGAGGAGCCACCAGCCACAGCACCACCAACTCG GCGGGCGTCCGCCATCCCTCGGGCTCTTAAACGTGAGAAACCCGCAGGCAGGAAAGAGACCCCAACCAGG GCGACACAGCCTGCCACACTCCCTGTGAGGGCGCAGCCGAGACTGATAGTAGATGAGACCCCACCCTGCT ATTCCCTGACTTCCTCGGCTAGTTCCCTCAGCGAGCCTGAGGCCTCTGAACAGCCAGCCTGCCACCCTCG AGTGGAGGAGCAGGGCAGTAAACAAGATAGTAGCCCAAGGGCAGAAGAGGAGCTCCTGCAGAGATGTATC AGTTTAGCTATGCCCAGGCGCCGGACCCAGGTACCCAGCTCACGGCGTCGCAAGCCCAGAGCTGTGAGGT CAGACATAAGGCCCACTGAGTTACCACAGAAATGCCGTGAAGAGGTTCCTGGTTCTGATCCAGCCTCTGA CCTAGATAGCGTCGAGTGGCAGGCTATCCAAGAGGGCGCAAACTCCATTGTCACGTGGCTGCATCAGGCA GCGGCCAAAGCCAGCCTGGAAGCTTCTTCTGAGTCTGACTCCCTCTTGTCTTTGGTGTCTGGGCTGTCAG CAAGCTCCACCCTCCAGCCCTCCAAGCTCAGAAAAGGGCGAAAGCCAGTGGCTGAGGCTGGGGGTGCCTG GCGTCCTGAGAAACGGGGCACAACTTCCACTAAGGGCAGTGGGAGTCCCCGGTTTCCTAGTGGCCCTGAG AAGGCAAAGGGTACCCAGAAAACGATGGCAGGGGAGTCAGCCATGCTCCGGGGAAGGACAGTGATCTACA CTGCCAGCCCTGCTTCCCGGGCTCAGTCCAAAGGTATTTCTGGACCTTGTTCCGCACCTAAGAAGATGGG GACATCTGGTACCACTCAGCCAGAAACTGCCACCAAAACCCCCAGCCCTGAGCAACAACGTTCTCGGAGC CTGCACCGACCAGGCAAGATCTCTGAGTTGGCAGCCTTGAGCCACCCGCCCAGGAGTGCTACTCCTCCAG CCCGCCTCACCAAGACCCCGTCTTCAAGCTCTTCACAGACCTCCCCGGCATCCCAGTCCCTGCCTAGGCG GTCCCCACTGGCCACTCCAACAGGAGGGCCTCTGCCTGGACCTGGGGGGTCCCCGGTGCCCAAGTCACCA GCCCGGGCTCTTCTGGCTAAGCAACACAAGACCCAGAAGTCACCCGTGCGTATCCCATTCATGCAAAGGC CAGCCAGGCGAGTGCCACCTCCACTGGCCAGGCCATCCCCAGAGCCTGGCTCCAGGGGCCGAGCTGGGGC TGAGGGAACTCCTGGGGCACGTGGCAGCCGCCTGGGCCTGGTACGTGTGGCCTCAACTCGCTCTAGCGGC AGTGAGTCCTCAGACCGCTCCGGCTTCAGAAGGCAGCTGACCTTCATTAAGGAGTCCCCAGGTCTCCTCC GGCGCCGTAGATCGGAGCTGTCCTCTGCGGACTCCACGGTCTCCACCTCCCAGACCGCTTCGCCCTGCCG CGGACGGCCCGCACTCCCCGCTGTCTTTCTCTGCTCCTCTCGCTGTGATGAGCTGCGAGCGTCTCCACGG CAGCCCCTGGCAGCACAGAGGGTCCCGCAGGCCAAGCCAGGTCTTGCCCCACGTGCGCCCAGACGCACCA GCTCCGAGAGCCCCTCACGCCTGCCTGTACGGGCGACCCCTGGGCGGCCCGAGACAGTCAAGAGGTACGC CTCCCTGCCACATATTAGTGTGTCCCGCAGGCCCGATAGCGCTGTCTCTGTGCCCACCACCCAGGCCAAT GCCACTCGCAGGGGAAGTGATGGCGAGGCCAGGCCGCTGCCCAGGGTGGCTGCGCCGGGTACGACCTGGC GTCGTATCAAAGATGAAGATGTCCCCCACATCCTGCGCAGCACACTGCCGGCCACTGCCCTGCCTCTCAG GGGCTCATCACCCGAAGACAGTCCTGCTGGAACTCCACACCGCAAGACTAGCGACGCAGTGGTACAGACA GAGGATGTGGCCACCTCTAAAACCAACTCCAGCACGTCGCCCAGCCTGGAGAGCAGGGATCCCCCACAGG CCCCGATCAGTGGCCCTGTTGCTCCACTGGGCAGCGATGTGGATGGACCAGTACTTGCCAAGCCTCCTGC CTCAGCGCCCTTCACCCATGAGGGTCTGAGTGTTGTCACGGGGGGCTTTCCTACTAGCAGGCATGGCTCC CCCAGTAGGGCTGCACGGGTTCCCCCCTTCAACTACGTGCCCAGCCCTATGGTAGTGGCTACAATGACCA GTGACTCAGCTGTGGAGAAAGCCCCTGTTACCTCTCCAGCCAGCCTCCTGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR215150 representing NM_001106769 Red=Cloning site Green=Tags(s) MASSVASYEQLVRQVEALKAENTHLRQELRDNSSHLSKLETETSGMKEVLKHLQGKLEQEARVLVSSGQT EVLEQLKALQTDISSLYNLKFHAPALGPEPAAQTPEGSPVHGPAPSKDSFGELSRATIRLLEELDQERCF LLSEIEKEEKEKLWYYSQLQGLSKRLDELPHVDTQFSMQMDLIRQQLEFEAQHIRSLMEERFGTSDEMVQ RAQIRASRLEQIDKELLEAQDRVQQTEPQALLAVKPVAVEEQEAEVPTHPEDVTPQPGNSKVEVVFWLLS MLATRDQEDTARTLLAMSSSPESCVAMRRSGCLPLLLQILHGTEAGSVGRTGTPGAPGAKDARMRANAAL HNIVFSQPDQGLARKEMRVLHVLEQIRAYCETCWDWLQARDSGTESGTGDTPVPIEPQICQAACAVMKLS FDEEYRRAMNELGGLQAVAELLQVDYEMHKMTQDPLNLALRRYAGMTLTNLTFGDVANKATLCARRGCME AIVAQLASESEELHQVVSSILRNLSWRADINSKKVLREVGSMTALMECVLRASKESTLKSVLSALWNLSA HSTENKAAICQVDGALGFLVSTLTYRCQGNSLAVIESGGGILRNVSSLIATREDYRQVLRDHNCLQTLLQ HLTSHSLTIVSNACGTLWNLSARSPRDQELLWDLGAVGMLRNLVHSKHKMIAMGSAAALRNLLAHRPAKY QAAAMAVSPGTCVPSLYVRKQRALEAELDTRHLVHALGHLEKQGLPEAETTSKKPLPPLRHLDGLVQDYA SDSGCFDDDDAPSLAAAATTAEPASPAVMSMFLGGPFLQGQALARTPPARQGGLEAEKETGGEAAVAAKA KAKLALAVARIDRLVEDISALHTSSDDSFSLSSGDPGQEAPREGRAQSCSPCRGTEGGRREAGSRAHPLL RLKAAHTSLSNDSLNSGSTSDGYCTREHMTPCPLAVLTEHRDGPVCGQTRPSRLDLDLPSRAELPARDTA ATDARVRTIKLSPTYQHVPLLDGAPGAGVRPLVGLGTSPGTRKQAWIPADSLSKVPEKLVASPLPVTSKV LQKLVAQDGPMSLSRCSSLSSLSSTGHAVPSQVENLDDSDSSLEELEEAGPGEAELDRAWRASGSTSLPV SIPAPQRGRSRGLGVEDATPSSSSENCVQETPLVLSRCSSVSSLGSFESRSIASSIPSDPCSALGSGTVS PSELPDSPGQTMPPSRSRTPPAPPGQPEASQFSLQWESYVKRFLDIADCRERCQPPSELDAGSVRFTVEK PDENFSCASSLSALALHELYVQQDVELRLRPPACPERAVGGGGGHRRRDEATSRLDGPAPAGSRARSAAD KELEALRECLGAAMPARLRKVASALVPGRRALPVPVYMLVPAPARGDDSGTDSAEGTPVNFSSAASLSDE TLQGPSRDKPARPGDRQKPTGRAAPARQTRGHRPKAAGAGAGKSTEHTRGANRNRAGLELPLSRPQSARS NRDGSCQTRTRGDGALQSLCLTTPTEEAVYCFYDSDEEPPATAPPTRRASAIPRALKREKPAGRKETPTR ATQPATLPVRAQPRLIVDETPPCYSLTSSASSLSEPEASEQPACHPRVEEQGSKQDSSPRAEEELLQRCI SLAMPRRRTQVPSSRRRKPRAVRSDIRPTELPQKCREEVPGSDPASDLDSVEWQAIQEGANSIVTWLHQA AAKASLEASSESDSLLSLVSGLSASSTLQPSKLRKGRKPVAEAGGAWRPEKRGTTSTKGSGSPRFPSGPE KAKGTQKTMAGESAMLRGRTVIYTASPASRAQSKGISGPCSAPKKMGTSGTTQPETATKTPSPEQQRSRS LHRPGKISELAALSHPPRSATPPARLTKTPSSSSSQTSPASQSLPRRSPLATPTGGPLPGPGGSPVPKSP ARALLAKQHKTQKSPVRIPFMQRPARRVPPPLARPSPEPGSRGRAGAEGTPGARGSRLGLVRVASTRSSG SESSDRSGFRRQLTFIKESPGLLRRRRSELSSADSTVSTSQTASPCRGRPALPAVFLCSSRCDELRASPR QPLAAQRVPQAKPGLAPRAPRRTSSESPSRLPVRATPGRPETVKRYASLPHISVSRRPDSAVSVPTTQAN ATRRGSDGEARPLPRVAAPGTTWRRIKDEDVPHILRSTLPATALPLRGSSPEDSPAGTPHRKTSDAVVQT EDVATSKTNSSTSPSLESRDPPQAPISGPVAPLGSDVDGPVLAKPPASAPFTHEGLSVVTGGFPTSRHGS PSRAARVPPFNYVPSPMVVATMTSDSAVEKAPVTSPASLLE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001106769
ORF Size	6843 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001106769.2, NP_001100239.2
RefSeq Size	9594 bp
RefSeq ORF	6846 bp
Locus ID	299611
MW	243.9 kDa

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