Hk1 (NM_012734) Rat Tagged ORF Clone
CAT#: RR214718
- TrueORF®
Hk1 (Myc-DDK-tagged ORF) - Rat hexokinase 1 (Hk1), nuclear gene encoding mitochondrial protein, (10 ug)
"NM_012734" in other vectors (3)
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CNY 11,880.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | HEXOKIN; HKI |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR214718 representing NM_012734
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCGCCGCGCAACTACTGGCCTATTACTTCACCGAGCTGAAGGATGACCAAGTCAAAAAGATTGACA AGTATCTGTACGCCATGCGGCTCTCTGATGAGATTCTGATAGATATCCTGACACGATTCAAGAAAGAGAT GAAGAATGGCCTCTCCCGGGATTATAATCCAACAGCCTCCGTCAAGATGCTGCCCACCTTCGTCCGGTCC ATTCCGGACGGCTCAGAAAAGGGGGATTTCATTGCCCTGGATCTCGGCGGGTCTTCCTTTCGAATCCTGC GGGTGCAGGTGAACCACGAGAAGAACCAGAACGTCAGCATGGAGTCTGAGATCTACGACACCCCAGAGAA CATCGTGCATGGCAGTGGAACCCAGCTTTTCGATCATGTCGCTGACTGCCTGGGAGACTTCATGGAGAAA AAGAAGATCAAGGACAAGAAGTTACCCGTGGGATTCACATTTTCCTTCCCCTGCCGACAATCCAAGATAG ATGAGGCTGTACTGATCACGTGGACAAAGCGGTTCAAAGCCAGTGGCGTGGAAGGAGCGGATGTGGTCAA GTTGCTGAATAAAGCCATTAAGAAGCGAGGGGACTATGATGCTAACATTGTCGCCGTGGTGAATGACACA GTAGGGACCATGATGACCTGCGGTTATGATGACCAACAGTGTGAAGTCGGCCTGATCATTGGCACAGGCA CCAATGCTTGCTACATGGAGGAACTGCGACACATCGACCTGGTGGAAGGCGACGAGGGGAGGATGTGTAT TAACACGGAATGGGGAGCCTTTGGGGATGATGGGTCCCTGGAAGACATCCGAACCGAGTTTGACAGAGAG TTAGACCGTGGATCTCTCAACCCTGGGAAGCAGCTGTTCGAGAAGATGGTGAGCGGCATGTACATGGGGG AGCTGGTCCGGCTAATCCTGGTGAAGATGGCCAAGGAAGGCCTCTTATTCGAAGGGCGCATCACTCCAGA GCTGCTCACGAGGGGAAAGTTCAACACTAGTGACGTGTCCGCCATTGAAAAGGATAAGGAAGGCATTCAA AATGCCAAGGAAATCTTAACCCGCTTGGGAGTGGAGCCGTCTGATGTTGACTGTGTGTCGGTCCAGCACA TCTGCACGATCGTCTCCTTCCGATCAGCCAACCTGGTGGCCGCCACGCTCGGTGCCATCTTGAACCGCCT GCGGGACAACAAGGGCACACCACGCCTGCGGACCACGGTTGGCGTGGACGGTTCTCTCTACAAGATGCAC CCACAGTACTCCCGGCGGTTCCACAAGACCCTGAGGCGCCTGGTGCCTGACTCCGACGTCCGTTTCCTCC TCTCAGAGAGTGGCACGGGCAAGGGGGCCGCCATGGTGACGGCAGTAGCCTACCGCCTGGCTGAGCAGCA CCGGCAGATTGAGGAAACCCTGGCCCACTTCCGCCTCAGCAAGCAGACGCTGATGGAGGTGAAGAAGAGG CTACGGACAGAGATGGAAATGGGGCTGAGGAAGGAGACCAACAGCAAAGCTACTGTCAAAATGCTGCCTT CTTTTGTCCGGAGCATCCCGGATGGGACTGAACACGGTGACTTCCTGGCCTTGGATCTTGGAGGAACGAA TTTCCGGGTTCTGCTGGTAAAGATCCGCAGTGGGAAAAAGAGAACAGTGGAAATGCACAACAAGATCTAC TCCATTCCCCTGGAAATCATGCAGGGCACCGGGGATGAGCTGTTTGACCACATCGTCTCCTGCATCTCTG ACTTCCTGGACTACATGGGGATCAAAGGCCCCCGGATGCCTCTGGGCTTCACCTTCTCATTTCCCTGCCA TCAGACGAACCTGGACTGTGGAATCTTGATCTCATGGACAAAGGGTTTCAAAGCCACTGACTGTGAGGGC CATGATGTAGCCTCCTTACTGAGGGATGCGGTGAAGAGGAGAGAGGAATTTGACTTGGATGTGGTGGCTG TGGTCAACGACACCGTGGGCACCATGATGACCTGTGCGTATGAAGAACCCACTTGCGAAATTGGACTCAT CGTGGGGACGGGCACCAATGCCTGCTACATGGAGGAGATGAAGAATGTGGAGATGGTGGAGGGGAACCAG GGCCAGATGTGCATCAACATGGAGTGGGGCGCCTTCGGTGACAATGGGTGTCTGGATGACATCAGAACAG ACTTTGACAAAGTGGTGGACGAATATTCTCTAAACTCTGGGAAACAAAGGTTTGAGAAAATGATCAGTGG GATGTACCTGGGTGAGATCGTCCGTAACATCCTGATTGACTTCACCAAGAAAGGCTTCCTCTTCCGGGGA CAGATCTCCGAACCACTCAAGACCCGAGGCATCTTTGAGACCAAGTTTCTCTCTCAGATTGAGAGTGACC GGTTAGCGCTGCTCCAGGTGCGGGCCATCCTTCAGCAGCTGGGTTTGAACAGCACGTGTGACGACAGTAT CCTGGTCAAGACCGTGTGTGGGGTGGTGTCCAAGAGGGCGGCTCAGCTGTGTGGTGCCGGCATGGCCGCC GTGGTGGAAAAGATCAGAGAGAACAGAGGCCTAGACCATCTGAATGTAACTGTGGGAGTGGATGGGACGC TCTACAAACTTCATCCACACTTCTCCAGAATCATGCACCAAACTGTGAAGGAACTGTCACCAAAGTGTAC CGTGTCCTTCCTCCTGTCTGAAGACGGCAGCGGCAAGGGGGCCGCCCTTATCACAGCTGTGGGCGTGCGG CTCAGAGGAGACCCTTCGATCGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR214718 representing NM_012734
Red=Cloning site Green=Tags(s) MIAAQLLAYYFTELKDDQVKKIDKYLYAMRLSDEILIDILTRFKKEMKNGLSRDYNPTASVKMLPTFVRS IPDGSEKGDFIALDLGGSSFRILRVQVNHEKNQNVSMESEIYDTPENIVHGSGTQLFDHVADCLGDFMEK KKIKDKKLPVGFTFSFPCRQSKIDEAVLITWTKRFKASGVEGADVVKLLNKAIKKRGDYDANIVAVVNDT VGTMMTCGYDDQQCEVGLIIGTGTNACYMEELRHIDLVEGDEGRMCINTEWGAFGDDGSLEDIRTEFDRE LDRGSLNPGKQLFEKMVSGMYMGELVRLILVKMAKEGLLFEGRITPELLTRGKFNTSDVSAIEKDKEGIQ NAKEILTRLGVEPSDVDCVSVQHICTIVSFRSANLVAATLGAILNRLRDNKGTPRLRTTVGVDGSLYKMH PQYSRRFHKTLRRLVPDSDVRFLLSESGTGKGAAMVTAVAYRLAEQHRQIEETLAHFRLSKQTLMEVKKR LRTEMEMGLRKETNSKATVKMLPSFVRSIPDGTEHGDFLALDLGGTNFRVLLVKIRSGKKRTVEMHNKIY SIPLEIMQGTGDELFDHIVSCISDFLDYMGIKGPRMPLGFTFSFPCHQTNLDCGILISWTKGFKATDCEG HDVASLLRDAVKRREEFDLDVVAVVNDTVGTMMTCAYEEPTCEIGLIVGTGTNACYMEEMKNVEMVEGNQ GQMCINMEWGAFGDNGCLDDIRTDFDKVVDEYSLNSGKQRFEKMISGMYLGEIVRNILIDFTKKGFLFRG QISEPLKTRGIFETKFLSQIESDRLALLQVRAILQQLGLNSTCDDSILVKTVCGVVSKRAAQLCGAGMAA VVEKIRENRGLDHLNVTVGVDGTLYKLHPHFSRIMHQTVKELSPKCTVSFLLSEDGSGKGAALITAVGVR LRGDPSIA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_012734 |
ORF Size | 2754 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_012734.1, NP_036866.1 |
RefSeq Size | 3653 bp |
RefSeq ORF | 2757 bp |
Locus ID | 25058 |
UniProt ID | P05708 |
MW | 102.4 kDa |
Gene Summary | a ATP:D-hexose 6-phosphotransferase involved in the phosphorylation of glucose to produce glucose-6-phosphate [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN214718 | Hk1 (untagged ORF) - Rat hexokinase 1 (Hk1), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 11,880.00 |
|
RR214718L3 | Lenti ORF clone of Hk1 (Myc-DDK-tagged ORF) - Rat hexokinase 1 (Hk1), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 13,490.00 |
|
RR214718L4 | Lenti ORF clone of Hk1 (mGFP-tagged ORF) - Rat hexokinase 1 (Hk1), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 14,920.00 |