Col2a1 (NM_012929) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CG2A1A; COLLII
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR214632 representing NM_012929 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCCGCCTCGGGGCTCCCCAGTCGCTGGTGCTGCTGACGCTGCTCATCGCCACGGTCCTACAATGTC AGGGCCAGGATGCCCGAAAATTAGGGCCAAAGGGGCAGAAAGGAGAACCTGGAGATATCAAAGATATCAT AGGACCTAAAGGACCTCCTGGCCCTCAGGGACCTGCCGGTGAACAAGGACCCAGAGGTGACCGTGGTGAC AAGGGAGAGAGGGGTGCACCTGGACCCCGTGGCAGAGATGGAGAACCTGGTACCCCTGGAAATCCTGGTC CCCCTGGCCCTCCAGGCCCCCCTGGTCCCCCTGGCCTTGGTGGAGGAAACTTTGCAGCCCAGATGGCTGG AGGATTTGACGAGAAGGCTGGTGGTGCCCAGATGGGAGTCATGCAAGGCCCCATGGGCCCCATGGGACCT CGTGGACCCCCAGGCCCCGCCGGTGCCCCCGGCCCTCAAGGATTTCAAGGCAATCCTGGTGAACCTGGCG AGCCTGGTGTCTCTGGTCCCATTGGTCCCCGAGGTCCTCCAGGTCCTGCTGGAAAACCTGGTGATGATGG TGAAGCTGGAAAGCCCGGAAAGGCCGGAGAAAGAGGCCTCCCTGGTCCTCAGGGTGCTCGTGGATTCCCG GGAACCCCCGGTCTTCCTGGTGTCAAGGGTCACAGAGGTTACCCAGGCCTCGATGGTGCTAAGGGAGAAG CTGGTGCTCCAGGTGTGAAGGGTGAGAGCGGTTCCCCTGGTGAGAACGGATCCCCAGGCCCAATGGGTCC CCGTGGCCTGCCTGGCGAGAGAGGACGGACTGGCCCTGCTGGTGCTGCTGGTGCTCGAGGCAACGACGGC CAGCCAGGCCCTGCTGGACCTCCGGGTCCTGTTGGTCCTGCAGGTGGTCCTGGCTTCCTTGGTGCTCCTG GTGCCAAGGGCGAAGCTGGTCCCACTGGTGCCCGTGGTCCTGAAGGTGCTCAAGGTTCTCGTGGCGAACC CGGCAATCCCGGATCCCCCGGGCCCGCAGGCGCTTCTGGTAACCCAGGGACTGATGGTATTCCCGGAGCC AAAGGATCTGCTGGCGCTCCTGGAATTGCTGGTGCCCCTGGCTTCCCTGGGCCCCGTGGCCCTCCCGGTC CTCAAGGTGCAACTGGTCCTCTGGGCCCCAAAGGTCAGACGGGTGAGCCCGGCATCGCTGGCTTCAAAGG TGAACAAGGCCCCAAGGGAGAGACTGGACCTGCTGGGCCCCAGGGAGCCCCTGGCCCTGCTGGTGAAGAA GGAAAACGAGGTGCTCGAGGAGAGCCCGGTGGCGCTGGACCTATCGGCCCCCCTGGAGAGAGAGGTGCTC CTGGCAACCGCGGTTTCCCAGGTCAAGATGGTCTGGCAGGTCCCAAGGGTGCCCCTGGAGAGCGAGGGCC TAGTGGCCTGGCTGGTCCTAAGGGTGCCAATGGTGACCCGGGTCGTCCTGGAGAACCTGGTCTTCCTGGA GCCAGGGGTCTTACTGGCCGCCCTGGTGATGCTGGTCCTCAAGGCAAAGTTGGTCCTTCTGGAGCCCCTG GAGAAGACGGTCGCCCTGGTCCTCCTGGTCCTCAGGGAGCTCGTGGGCAGCCTGGCGTCATGGGTTTCCC TGGCCCCAAAGGAGCCAATGGCGAGCCTGGCAAAGCTGGTGAGAAAGGACTGGCTGGTGCTCCTGGTCTG AGAGGTCTTCCTGGCAAAGATGGTGAGACAGGAGCCGCAGGACCCCCCGGCCCCAGCGGACCTGCTGGTG AACGAGGCGAGCAGGGTGCTCCTGGGCCATCAGGGTTCCAGGGACTTCCTGGCCCTCCTGGTCCCCCGGG TGAAGGTGGAAAGCAAGGTGACCAGGGTATTCCTGGTGAAGCTGGAGCCCCTGGCCTCGTTGGTCCCCGG GGTGAACGAGGTTTCCCAGGTGAACGTGGCTCTCCCGGTGCTCAGGGCCTCCAGGGTCCCCGAGGCCTCC CTGGCACTCCTGGTACTGATGGTCCCAAAGGTGCGGCTGGCCCAGATGGTCCCCCTGGGGCTCAGGGCCC TCCAGGTCTACAGGGAATGCCTGGTGAGAGGGGAGCAGCTGGTATTGCTGGACCCAAGGGAGACAGAGGT GATGTTGGCGAGAAAGGCCCAGAGGGAGCTCCTGGAAAAGATGGTGGCCGAGGTCTTACTGGGCCCATCG GGCCCCCAGGACCAGCAGGTGCCAATGGCGAGAAGGGAGAAGTCGGACCTCCTGGTCCTTCAGGAAGTAC TGGAGCTCGAGGCGCCCCGGGTGAGCGCGGAGAGACTGGGCCACCTGGACCTGCTGGATTCGCTGGCCCT CCTGGTGCTGATGGCCAGCCTGGTGCCAAGGGCGATCAAGGAGAAGCTGGACAGAAAGGTGACGCTGGTG CCCCTGGCCCACAAGGCCCCTCAGGAGCTCCCGGGCCACAGGGTCCTACTGGAGTGACTGGTCCTAAGGG AGCCCGGGGCGCCCAAGGCCCACCGGGAGCCACCGGATTCCCTGGAGCTGCTGGCCGAGTTGGACCCCCA GGTTCTAATGGCAACCCTGGGCCCGCCGGTCCCCCTGGTCCTGCTGGAAAAGATGGTCCCAAAGGTGCTC GAGGAGACACTGGTGCCCCTGGCAGAGCTGGTGACCCTGGACTTCAAGGACCCGCAGGAGCTCCTGGAGA GAAAGGCGAACCTGGAGATGACGGTCCCTCTGGTTCTGATGGTCCCCCAGGTCCCCAGGGGCTGGCTGGT CAAAGGGGCATTGTTGGTCTGCCTGGTCAGCGTGGTGAGAGAGGATTCCCAGGCCTTCCCGGCCCATCGG GTGAGCCCGGCAAGCAGGGTGCACCTGGTGCATCTGGAGACAGAGGTCCTCCTGGTCCTGTGGGGCCTCC TGGCTTGACAGGACCTGCAGGTGAACCTGGACGAGAGGGCAGCCCTGGTGCTGATGGACCCCCTGGCAGA GATGGTGCGGCTGGAGTCAAGGGTGATCGTGGTGAGACTGGTGCACTTGGTGCTCCTGGAGCTCCTGGGC CCCCAGGCTCTCCTGGTCCTGCTGGCCCAACTGGCAAACAAGGAGACAGAGGAGAGGCTGGTGCACAAGG TCCTATGGGCCCCTCAGGACCTGCTGGAGCCCGTGGAATTGCTGGCCCTCAAGGCCCCCGAGGTGACAAA GGAGAAGCTGGAGAGCCTGGCGAGAGAGGACTGAAGGGGCACCGAGGTTTCACTGGACTGCAGGGTCTGC CTGGCCCCCCGGGTCCTTCTGGAGATCAGGGTACTTCTGGCCCTGCTGGTCCTTCCGGCCCTAGAGGTCC ACCTGGCCCTGTTGGTCCCTCTGGCAAAGATGGCTCTAATGGAATCCCTGGCCCCATCGGGCCTCCAGGT CCCCGTGGACGCTCAGGAGAAACTGGCCCTGCTGGTCCTCCTGGAAATCCTGGTCCCCCTGGCCCTCCGG GTCCTCCTGGTCCTGGCATCGACATGTCAGCCTTTGCTGGCTTAGGACAGAGAGAGAAGGGCCCCGATCC CCTGCAGTACATGCGGGCCGACGAGGCAGACAGTACCTTGAGACAGCATGACGTCGAGGTGGACGCCACG CTCAAGTCGCTGAACAACCAGATCGAGAGCATCCGCAGCCCTGATGGCTCCCGCAAGAATCCCGCTCGCA CCTGCCAGGACCTGAAACTCTGCCACCCAGAGTGGAAGAGCGGAGACTACTGGATTGATCCCAACCAGGG CTGCACCTTGGACGCCATGAAAGTCTTCTGCAACATGGAGACTGGCGAGTCTTGCGTCTACCCCAACCCA GCGACTGTGCCTCGGAAGAACTGGTGGAGCAGCAAGAGCAAGGAGAAGAAGCACATCTGGTTTGGAGAGA CCATGAACGGCGGCTTCCACTTCAGCTACGGCGACGGCAACCTGGCTCCCAACACCGCTAACGTCCAGAT GACTTTCCTCCGTCTACTGTCCACTGAGGGCTCCCAGAACATCACCTACCACTGTAAGAACAGCATTGCC TACCTGGACGAAGCAGCCGGCAACCTCAAGAAGGCCTTGCTCATCCAGGGCTCCAATGATGTGGAGATGA GGGCCGAGGGCAACAGCAGGTTCACGTACACTGCCCTGAAGGATGGCTGCACGAAACACACCGGTAAGTG GGGCAAGACCATCATCGAGTACCGATCACAGAAGACCTCACGCCTTCCCATTGTTGACATTGCACCCATG GACATCGGAGGGCCTGATCAGGAATTTGGTGTGGACATAGGGCCTGTCTGTTTCTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR214632 representing NM_012929 Red=Cloning site Green=Tags(s) MIRLGAPQSLVLLTLLIATVLQCQGQDARKLGPKGQKGEPGDIKDIIGPKGPPGPQGPAGEQGPRGDRGD KGERGAPGPRGRDGEPGTPGNPGPPGPPGPPGPPGLGGGNFAAQMAGGFDEKAGGAQMGVMQGPMGPMGP RGPPGPAGAPGPQGFQGNPGEPGEPGVSGPIGPRGPPGPAGKPGDDGEAGKPGKAGERGLPGPQGARGFP GTPGLPGVKGHRGYPGLDGAKGEAGAPGVKGESGSPGENGSPGPMGPRGLPGERGRTGPAGAAGARGNDG QPGPAGPPGPVGPAGGPGFLGAPGAKGEAGPTGARGPEGAQGSRGEPGNPGSPGPAGASGNPGTDGIPGA KGSAGAPGIAGAPGFPGPRGPPGPQGATGPLGPKGQTGEPGIAGFKGEQGPKGETGPAGPQGAPGPAGEE GKRGARGEPGGAGPIGPPGERGAPGNRGFPGQDGLAGPKGAPGERGPSGLAGPKGANGDPGRPGEPGLPG ARGLTGRPGDAGPQGKVGPSGAPGEDGRPGPPGPQGARGQPGVMGFPGPKGANGEPGKAGEKGLAGAPGL RGLPGKDGETGAAGPPGPSGPAGERGEQGAPGPSGFQGLPGPPGPPGEGGKQGDQGIPGEAGAPGLVGPR GERGFPGERGSPGAQGLQGPRGLPGTPGTDGPKGAAGPDGPPGAQGPPGLQGMPGERGAAGIAGPKGDRG DVGEKGPEGAPGKDGGRGLTGPIGPPGPAGANGEKGEVGPPGPSGSTGARGAPGERGETGPPGPAGFAGP PGADGQPGAKGDQGEAGQKGDAGAPGPQGPSGAPGPQGPTGVTGPKGARGAQGPPGATGFPGAAGRVGPP GSNGNPGPAGPPGPAGKDGPKGARGDTGAPGRAGDPGLQGPAGAPGEKGEPGDDGPSGSDGPPGPQGLAG QRGIVGLPGQRGERGFPGLPGPSGEPGKQGAPGASGDRGPPGPVGPPGLTGPAGEPGREGSPGADGPPGR DGAAGVKGDRGETGALGAPGAPGPPGSPGPAGPTGKQGDRGEAGAQGPMGPSGPAGARGIAGPQGPRGDK GEAGEPGERGLKGHRGFTGLQGLPGPPGPSGDQGTSGPAGPSGPRGPPGPVGPSGKDGSNGIPGPIGPPG PRGRSGETGPAGPPGNPGPPGPPGPPGPGIDMSAFAGLGQREKGPDPLQYMRADEADSTLRQHDVEVDAT LKSLNNQIESIRSPDGSRKNPARTCQDLKLCHPEWKSGDYWIDPNQGCTLDAMKVFCNMETGESCVYPNP ATVPRKNWWSSKSKEKKHIWFGETMNGGFHFSYGDGNLAPNTANVQMTFLRLLSTEGSQNITYHCKNSIA YLDEAAGNLKKALLIQGSNDVEMRAEGNSRFTYTALKDGCTKHTGKWGKTIIEYRSQKTSRLPIVDIAPM DIGGPDQEFGVDIGPVCFL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_012929
ORF Size	4257 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_012929.1, NP_037061.1
RefSeq Size	4538 bp
RefSeq ORF	4260 bp
Locus ID	25412
UniProt ID	P05539
MW	134.6 kDa
Gene Summary	structural component of the cartilage extracellular matrix [RGD, Feb 2006]

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