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Nefh (NM_012607) Rat Tagged ORF Clone
CAT＃: RR214621
- TrueORF®
Nefh (Myc-DDK-tagged ORF) - Rat neurofilament, heavy polypeptide (Nefh), (10 ug)
"NM_012607" in other vectors (3)
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CNY 17,100.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Nfh |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR214621 representing NM_012607
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGAGCTTCGGCGGCGCCGATGCGCTGCTGGGCGCCCCGTTCGCGCCGCTGCACGGAGGAGGCAGCC TGCACTACGCGCTGAGCCGCAAGGCAGGCGCCGGCGGCACCCGCTCCGCGGCCGGCTCCTCCAGCGGCTT CCATTCGTGGGCGCGGACGTCGGTGAGCTCCGTGTCCGCCTCGCCCAGCCGCTTCCGCGGCGCCGGCGCC GCCTCGAGCACCGACTCGCTAGACACCCTAAGCAATGGCCCAGAGGGCTGCGTGGTGGCGGCGGTGGCTG CGCGCAGCGAGAAGGAGCAGCTGCAGGCGCTCAACGACCGCTTCGCGGGCTACATCGATAAGGTGAGGCA GCTTGAGGCGCACAACCGCAGCCTGGAAGGCGAGGCGGCGGCGCTGCGGCAGCAGCAAGCCGGCCGCGCC GCCATGGGCGAGCTGTACGAGCGCGAGGTGCGCGAGATGCGCGGGGCCGTGCTGCGCCTGGGGGCGGCGC GCGGGCAGCTGCGCCTGGAGCAGGAGCACTTGCTGGAGGACATCGCGCACGTCCGCCAGCGGCTGGACGA GGAGGCCCGGCAGCGCGAGGAAGCGGAGGCGGCGGCCCGCGCCCTAGCCCGCTTCGCGCAGGAGGCGGAA GCGGCGCGCGTGGAGCTGCAGAAGAAGGCGCAGGCGCTGCAGGAAGAGTGCGGCTACCTGCGGCGCCACC ACCAGGAGGAGGTGGGCGAGCTGCTCGGTCAGATTCAGGGCTGCGGTGCCGCGCAGGCGCAGGCTCAGGC CGAGGCTCGGGACGCCCTCAAGTGCGACGTGACGTCGGCGCTGCGGGAGATCCGCGCGCAGCTCGAAGGA CACACGGTGCAGAGTACGCTGCAGTCAGAGGAGTGGTTCCGAGTGAGATTGGACCGACTCTCAGAGGCAG CCAAAGTGAACACGGATGCTATGCGCTCTGCCCAAGAGGAGATAACTGAGTACCGGCGGCAGCTGCAGGC CAGGACCACAGAGTTGGAGGCACTGAAAAGCACCAAGGAGTCACTGGAGAGGCAGCGCTCTGAGCTGGAG GACCGTCATCAGGTAGACATGGCCTCCTACCAGGATGCAATTCAGCAGCTGGACAATGAGCTGAGAAACA CCAAATGGGAGATGGCCGCGCAGCTCCGAGAGTACCAGGACCTGCTCAACGTCAAGATGGCCCTGGATAT TGAGATCGCTGCTTACAGAAAACTCCTGGAAGGCGAAGAGTGTCGGATTGGCTTTGGACCCAGTCCCTTC TCTCTTACTGAGGGACTCCCAAAAATTCCCTCCATGTCCACTCACATAAAAGTCAAAAGCGAAGAGAAGA TAAAAGTAGTAGAAAAATCGGAGAAGGAAACCGTCATTGTAGAGGAACAGACAGAAGAGATCCAGGTGAC AGAAGAAGTGACAGAAGAGGAGGACAAAGAGGCCCAAGGGGAGGAAGAAGAAGAGGCAGAAGAGGGAGGA GAAGAAGCAGCAACTACGTCTCCCCCTGCAGAAGAGGCTGCATCTCCAGAAAAGGAAACCAAGTCTCCTG TGAAAGAAGAGGCCAAGTCCCCAGCTGAGGCCAAGTCCCCAGCTGAGGCCAAGTCACCAGCTGAGGCCAA GTCCCCAGCTGAGGTCAAATCTCCAGCTGTGGCCAAGTCCCCAGCTGAAGTCAAATCTCCAGCTGAGGTC AAATCTCCAGCTGAGGCCAAGTCACCAGCTGAGGCCAAGTCACCAGCTGAGGTCAAATCTCCAGCTACAG TGAAGTCTCCAGGTGAGGCCAAGTCCCCAGCTGAGGCCAAGTCACCAGCTGAGGTCAAATCTCCAGTGGA GGCCAAGTCACCAGCTGAGGCCAAGTCTCCAGCTTCAGTGAAGTCCCCAGGTGAGGCCAAGTCACCAGCT GAGGCCAAGTCACCAGCTGAGGTCAAATCTCCAGCTACAGTGAAGTCCCCAGTTGAGGCCAAGTCACCAG CTGAGGTCAAATCTCCAGTTACAGTGAAGTCCCCAGCTGAGGCCAAGTCACCAGTTGAGGTCAAATCTCC AGCTTCGGTGAAGTCCCCAAGTGAAGCCAAGTCACCAGCTGGAGCCAAGTCACCAGCTGAGGCCAAGTCA CCAGTTGTGGCCAAATCACCAGCTGAGGCCAAGTCACCAGCTGAGGCCAAGCCTCCAGCTGAGGCCAAGT CACCAGCTGAGGCCAAGTCTCCAGCTGAGGCCAAGTCTCCAGCTGAGGCCAAGTCACCAGCTGAGGCCAA GTCACCTGTTGAGGTAAAATCTCCAGAGAAGGCCAAGAGCCCCGTGAAGGAAGGTGCAAAATCCCTAGCT GAGGCCAAGTCCCCTGAGAAGGCCAAGTCCCCTGTGAAGGAAGAGATCAAGCCTCCAGCTGAGGTGAAAT CCCCCGAGAAGGCCAAGAGCCCCATGAAGGAGGAGGCCAAGTCTCCTGAGAAGGCCAAGACTCTGGATGT GAAGTCTCCAGAAGCCAAGACTCCAGCGAAGGAGGAAGCAAAGCGCCCCGCAGACATCAGATCCCCTGAG CAGGTCAAAAGTCCTGCCAAGGAGGAGGCCAAGTCCCCCGAGAAGGAAGAGACCAGGACTGAAAAGGTGG CTCCCAAGAAGGAAGAGGTGAAGTCCCCTGTGGAGGAGGTAAAAGCCAAAGAACCCCCAAAGAAGGTGGA GGAGGAGAAGACACCAGCCACACCAAAGACAGAGGTGAAGGAGAGCAAGAAAGATGAAGCTCCCAAGGAG GCCCAGAAGCCCAAGGCGGAGGAGAAGGAGCCTCTCACAGAAAAGCCCAAGGACTCTCCGGGGGAAGCCA AGAAGGAAGAGGCTAAAGAGAAGAAGGCGGCGGCCCCAGAGGAGGAGACGCCCGCCAAGTTGGGCGTGAA GGAAGAGGCTAAACCCAAAGAGAAGGCAGAAGACGCCAAGGCCAAAGAACCTAGCAAACCCTCAGAGAAG GAGAAACCGAAGAAGGAGGAGGTGCCGGCAGCACCAGAGAAGAAAGACACCAAGGAGGAGAAGACTACGG AGTCCAAGAAGCCTGAGGAGAAACCCAAAATGGAGGCCAAGGCCAAGGAGGAGGACAAGGGCCTTCCCCA AGAGCCTAGCAAACCCAAGACAGAAAAGGCTGAAAAGTCCTCTAGCACAGACCAAAAAGACAGCCAGCCC TCAGAGAAGGCCCCAGAGGACAAGGCTGCCAAGGGAGACAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR214621 representing NM_012607
Red=Cloning site Green=Tags(s) MMSFGGADALLGAPFAPLHGGGSLHYALSRKAGAGGTRSAAGSSSGFHSWARTSVSSVSASPSRFRGAGA ASSTDSLDTLSNGPEGCVVAAVAARSEKEQLQALNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRA AMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDEEARQREEAEAAARALARFAQEAE AARVELQKKAQALQEECGYLRRHHQEEVGELLGQIQGCGAAQAQAQAEARDALKCDVTSALREIRAQLEG HTVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEITEYRRQLQARTTELEALKSTKESLERQRSELE DRHQVDMASYQDAIQQLDNELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPSPF SLTEGLPKIPSMSTHIKVKSEEKIKVVEKSEKETVIVEEQTEEIQVTEEVTEEEDKEAQGEEEEEAEEGG EEAATTSPPAEEAASPEKETKSPVKEEAKSPAEAKSPAEAKSPAEAKSPAEVKSPAVAKSPAEVKSPAEV KSPAEAKSPAEAKSPAEVKSPATVKSPGEAKSPAEAKSPAEVKSPVEAKSPAEAKSPASVKSPGEAKSPA EAKSPAEVKSPATVKSPVEAKSPAEVKSPVTVKSPAEAKSPVEVKSPASVKSPSEAKSPAGAKSPAEAKS PVVAKSPAEAKSPAEAKPPAEAKSPAEAKSPAEAKSPAEAKSPAEAKSPVEVKSPEKAKSPVKEGAKSLA EAKSPEKAKSPVKEEIKPPAEVKSPEKAKSPMKEEAKSPEKAKTLDVKSPEAKTPAKEEAKRPADIRSPE QVKSPAKEEAKSPEKEETRTEKVAPKKEEVKSPVEEVKAKEPPKKVEEEKTPATPKTEVKESKKDEAPKE AQKPKAEEKEPLTEKPKDSPGEAKKEEAKEKKAAAPEEETPAKLGVKEEAKPKEKAEDAKAKEPSKPSEK EKPKKEEVPAAPEKKDTKEEKTTESKKPEEKPKMEAKAKEEDKGLPQEPSKPKTEKAEKSSSTDQKDSQP SEKAPEDKAAKGDK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012607 |
| ORF Size | 3192 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012607.2, NP_036739.2 |
| RefSeq Size | 3843 bp |
| RefSeq ORF | 3195 bp |
| Locus ID | 24587 |
| MW | 114.4 kDa |
| Gene Summary | an intermediate filament protein found primarily in neurons [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN214621 | Nefh (untagged ORF) - Rat neurofilament, heavy polypeptide (Nefh), (10 ug) |
CNY 17,100.00 |
|
| RR214621L3 | Lenti ORF clone of Nefh (Myc-DDK-tagged ORF) - Rat neurofilament, heavy polypeptide (Nefh), (10 ug) |
CNY 18,720.00 |
|
| RR214621L4 | Lenti ORF clone of Nefh (mGFP-tagged ORF) - Rat neurofilament, heavy polypeptide (Nefh), (10 ug) |
CNY 20,430.00 |
