Mgll (NM_138502) Rat Tagged ORF Clone
CAT#: RR214599
- TrueORF®
Mgll (Myc-DDK-tagged ORF) - Rat monoglyceride lipase (Mgll), (10 ug)
"NM_138502" in other vectors (3)
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CNY 3,600.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | MAGL |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR214599 representing NM_138502
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGAGGCAAGTTCACCCAGGCGAACTCCACAGAACGTCCCCTACCAGGACCTTCCTCACCTGGTCA ATGCGGATGGACAGTACCTCTTTTGTAGGTACTGGAAGCCCAGTGGCACACCCAAGGCCCTCATCTTCGT GTCCCATGGAGCTGGGGAACACTGTGGCCGTTATGACGAGCTGGCTCAGATGTTGAAGAGGCTGGACATG CTGGTGTTTGCCCATGACCATGTTGGCCATGGGCAGAGCGAGGGAGAGAGGATGGTGGTATCGGACTTCC AAGTTTTTGTCAGAGATTTGTTGCAGCACGTGAACACCGTCCAGAAGGACTACCCCGAGGTCCCCGTCTT CCTCCTGGGCCACTCCATGGGCGGTGCCATCTCCATCCTAGCAGCTGCAGAGAGACCAACCCACTTTTCT GGCATGATCCTAATTTCACCTCTGATCCTTGCCAATCCGGAATCTGCATCGACTTTGAAGGTCCTTGCTG CCAAACTGCTCAATTTTGTCCTGCCAAACATATCCTTGGGGCGCATCGACTCCAGCGTGCTGTCTCGGAA CAAGTCGGAGGTTGACCTGTACAACTCCGACCCACTCATCTGCCACGCAGGGGTGAAGGTATGCTTTGGC ATCCAGCTGCTGAACGCTGTCTCGAGGGTGGAGCGAGCAATGCCCAGGCTGACACTGCCGTTCCTGCTGC TGCAGGGTTCTGCTGACCGGCTTTGCGACAGCAAAGGTGCCTACCTGCTCATGGAATCATCCCCGAGTCA GGACAAAACACTCAAGATGTATGAAGGTGCCTACCATGTTCTCCACAAGGAGCTTCCGGAAGTGACCAAC TCTGTCCTCCATGAAATAAATACGTGGGTGTCTCACAGGATAGCAGTGGCAGGAGCTAGGTGTCTACCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR214599 representing NM_138502
Red=Cloning site Green=Tags(s) MPEASSPRRTPQNVPYQDLPHLVNADGQYLFCRYWKPSGTPKALIFVSHGAGEHCGRYDELAQMLKRLDM LVFAHDHVGHGQSEGERMVVSDFQVFVRDLLQHVNTVQKDYPEVPVFLLGHSMGGAISILAAAERPTHFS GMILISPLILANPESASTLKVLAAKLLNFVLPNISLGRIDSSVLSRNKSEVDLYNSDPLICHAGVKVCFG IQLLNAVSRVERAMPRLTLPFLLLQGSADRLCDSKGAYLLMESSPSQDKTLKMYEGAYHVLHKELPEVTN SVLHEINTWVSHRIAVAGARCLP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_138502 |
ORF Size | 909 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_138502.2, NP_612511.1 |
RefSeq Size | 1234 bp |
RefSeq ORF | 912 bp |
Locus ID | 29254 |
UniProt ID | Q8R431 |
MW | 33.9 kDa |
Gene Summary | serine hydrolase that converts monoglycerides to fatty acid and glycerol; may play a role in retrograde signaling at central synapses and other forms of short-range neuronal communication [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN214599 | Mgll (untagged ORF) - Rat monoglyceride lipase (Mgll), (10 ug) |
CNY 3,990.00 |
|
RR214599L3 | Lenti ORF clone of Mgll (Myc-DDK-tagged ORF) - Rat monoglyceride lipase (Mgll), (10 ug) |
CNY 6,080.00 |
|
RR214599L4 | Lenti ORF clone of Mgll (mGFP-tagged ORF) - Rat monoglyceride lipase (Mgll), (10 ug) |
CNY 6,650.00 |