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Mapk3 (NM_017347) Rat Tagged ORF Clone
CAT＃: RR214231
- TrueORF®
Mapk3 (Myc-DDK-tagged ORF) - Rat mitogen activated protein kinase 3 (Mapk3), (10 ug)
"NM_017347" in other vectors (3)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Erk-1; ERK1; ERT2; Esrk1; MAPK1; MNK1; p44; p44erk1; p44mapk; Prkm3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR214231 representing NM_017347
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGCGGCGGCGGCTCCGGGGGGCGGGGGCGGGGAGCCCAGGGGAACTGCTGGGGTCGTCCCGG TGGTCCCCGGGGAGGTGGAGGTGGTGAAGGGGCAGCCATTCGACGTGGGCCCACGCTACACGCAGCTGCA GTACATCGGCGAGGGCGCGTACGGCATGGTCAGCTCAGCATATGACCACGTGCGCAAGACCAGAGTGGCT ATCAAGAAGATCAGCCCCTTCGAGCATCAAACCTACTGTCAGCGCACGCTGAGAGAAATCCAGATCTTGC TCGGATTCCGCCATGAGAATGTCATAGGCATCCGAGACATCCTCAGAGCACCCACCCTGGAAGCCATGAG AGATGTTTACATTGTTCAGGACCTCATGGAGACGGACCTGTACAAGCTGCTAAAGAGCCAGCAGCTGAGC AATGACCACATCTGCTACTTCCTCTACCAGATCCTCCGGGGCCTCAAGTACATACACTCGGCCAATGTGC TGCACCGGGACCTGAAGCCCTCCAATCTGCTTATCAACACCACCTGCGACCTTAAGATCTGTGATTTTGG CCTTGCCCGGATTGCTGACCCTGAGCACGACCACACTGGCTTTCTGACCGAGTATGTGGCCACACGCTGG TACCGAGCCCCAGAGATCATGCTTAACTCCAAGGGCTACACCAAATCCATTGACATCTGGTCTGTGGGCT GCATTCTGGCTGAGATGCTCTCCAACCGGCCTATCTTCCCCGGCAAGCACTACCTGGACCAGCTCAACCA CATTCTAGGTATACTGGGTTCCCCATCCCAAGAGGACCTAAATTGTATCATTAACATGAAGGCCCGAAAC TACCTACAGTCTCTGCCCTCTAAAACCAAGGTGGCTTGGGCCAAGCTTTTTCCCAAATCTGACTCCAAAG CTCTTGACCTGCTGGACCGGATGTTAACCTTTAACCCAAACAAGCGCATCACAGTAGAGGAAGCACTGGC TCACCCTTACCTGGAACAGTACTATGATCCGACAGATGAACCAGTGGCTGAGGAGCCATTCACCTTTGAC ATGGAGCTGGATGATCTCCCCAAGGAGCGGCTGAAGGAGCTGATCTTCCAAGAGACAGCCCGCTTCCAGC CAGGGGCACCAGAGGCCCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR214231 representing NM_017347
Red=Cloning site Green=Tags(s) MAAAAAAPGGGGGEPRGTAGVVPVVPGEVEVVKGQPFDVGPRYTQLQYIGEGAYGMVSSAYDHVRKTRVA IKKISPFEHQTYCQRTLREIQILLGFRHENVIGIRDILRAPTLEAMRDVYIVQDLMETDLYKLLKSQQLS NDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLINTTCDLKICDFGLARIADPEHDHTGFLTEYVATRW YRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINMKARN YLQSLPSKTKVAWAKLFPKSDSKALDLLDRMLTFNPNKRITVEEALAHPYLEQYYDPTDEPVAEEPFTFD MELDDLPKERLKELIFQETARFQPGAPEAP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017347 |
| ORF Size | 1140 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017347.2, NP_059043.1 |
| RefSeq Size | 1875 bp |
| RefSeq ORF | 1143 bp |
| Locus ID | 50689 |
| UniProt ID | P21708 |
| MW | 43 kDa |
| Gene Summary | kinase involved in intracellular signalling; component of Mapk signalling pathway [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN214231 | Mapk3 (untagged ORF) - Rat mitogen activated protein kinase 3 (Mapk3), (10 ug) |
CNY 3,990.00 |
|
| RR214231L3 | Lenti ORF clone of Mapk3 (Myc-DDK-tagged ORF) - Rat mitogen activated protein kinase 3 (Mapk3), (10 ug) |
CNY 6,080.00 |
|
| RR214231L4 | Lenti ORF clone of Mapk3 (mGFP-tagged ORF) - Rat mitogen activated protein kinase 3 (Mapk3), (10 ug) |
CNY 6,650.00 |
