Ppid (NM_001004279) Rat Tagged ORF Clone

CAT#: RR214228

  • TrueORF®

Ppid (Myc-DDK-tagged ORF) - Rat peptidylprolyl isomerase D (cyclophilin D) (Ppid), (10 ug)



  "NM_001004279" in other vectors (5)

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CNY 4,024.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Synonyms Cyp-40; CypD
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR214228 representing NM_001004279
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCCACCCATCCCCAGCAGGCAAGCCCTCCAATTCCAAGAACCCGCGAGTCTTCTTTGACGTGGACA
TCGGCGGGGAGCGAGTTGGACGAATTGTTTTAGAATTGTTTGCAGATATTGTTCCTAAAACTGCAGAAAA
TTTTCGTGCATTGTGTACAGGAGAAAAGGGCACTGGACCGACAACTGGGAAACCTCTGCATTTTAAAGGA
TGCCCTTTCCACCGAATTATTAAGAAATTTATGATTCAGGGTGGAGACTTCTCAAATCAGAATGGGACAG
GTGGAGAAAGTATTTATGGTGAAAAATTTGAAGATGAGAATTTTCATTATAAGCATGATCGGGAGGGTTT
GCTGAGCATGGCAAATGCAGGCCCCAATACAAATGGTTCTCAGTTCTTTATCACAACAGTTCCAACTCCT
CATTTGGATGGGAAACATGTGGTATTTGGTCAAGTAATAAAAGGACTAGGTGTGGCAAGGATGCTTGAAA
ATGTAGAAGTGAATGGTGAAAAACCTGCCAAACTCTGTGTTATTGCAGAATGTGGAGAATTGAAGGAAGG
GGATGAATGGGGAATATTCCCCAAAGATGGCTCTGGGGATAGTCATCCAGATTTCCCTGAGGATGCAGAC
ATAGACTTAAAGGATGTAGATAAAATTTTATTAATATCTGAAGACTTAAAAAACATTGGAAATACTTTTT
TCAAGTCTCAAAACTGGGAGATGGCTATTAAAAAATATGCAAAGGTTTTAAGGTACTTGGATAGTTCAAA
GGCTGTTATTGAGAAAGCAGATGTATCCAGACTGCAACCCATAGCCTTAAGTTGTGTGCTGAATATTGGT
GCTTGTAAATTGAAGATGTCAAATTGGCAGGGGGCAATTGACAGTTGCTTGGAGGCTCTTGAAATGGACC
CTTCAAACACTAAAGCACTATACCGAAAAGCACAAGGATGGCAAGGATTGAAAGAATATGATCAAGCATT
GGCTGATCTTAAGAAGGCGCAGGAGATAGCCCCAGGAGATAAAGCTATCCAGGCAGAATTGCTTAAAGTC
AAACAAATGATAAAGGCACAGAAAGATAAAGAGAAGGCAGTGTATGCAAAAATGTTTGCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR214228 representing NM_001004279
Red=Cloning site Green=Tags(s)

MSHPSPAGKPSNSKNPRVFFDVDIGGERVGRIVLELFADIVPKTAENFRALCTGEKGTGPTTGKPLHFKG
CPFHRIIKKFMIQGGDFSNQNGTGGESIYGEKFEDENFHYKHDREGLLSMANAGPNTNGSQFFITTVPTP
HLDGKHVVFGQVIKGLGVARMLENVEVNGEKPAKLCVIAECGELKEGDEWGIFPKDGSGDSHPDFPEDAD
IDLKDVDKILLISEDLKNIGNTFFKSQNWEMAIKKYAKVLRYLDSSKAVIEKADVSRLQPIALSCVLNIG
ACKLKMSNWQGAIDSCLEALEMDPSNTKALYRKAQGWQGLKEYDQALADLKKAQEIAPGDKAIQAELLKV
KQMIKAQKDKEKAVYAKMFA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001004279
ORF Size 1110 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001004279.1, NP_001004279.1
RefSeq Size 1353 bp
RefSeq ORF 1113 bp
Locus ID 361967
UniProt ID Q6DGG0
MW 40.8 kDa
Gene Summary PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding. Proposed to act as a co-chaperone in HSP90 complexes such as in unligated steroid receptors heterocomplexes. Different co-chaperones seem to compete for association with HSP90 thus establishing distinct HSP90-co-chaperone-receptor complexes with the potential to exert tissue-specific receptor activity control. May have a preference for estrogen receptor complexes and is not found in glucocorticoid receptor complexes. May be involved in cytoplasmic dynein-dependent movement of the receptor from the cytoplasm to the nucleus. May regulate MYB by inhibiting its DNA-binding activity. Involved in regulation of AHR signaling by promoting the formation of the AHR:ARNT dimer; the function is independent of HSP90 but requires the chaperone activity region. Involved in regulation of UV radiation-induced apoptosis.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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