Prrx1 (NM_153821) Rat Tagged ORF Clone
CAT#: RR214196
- TrueORF®
Prrx1 (Myc-DDK-tagged ORF) - Rat paired related homeobox 1 (Prrx1), (10 ug)
"NM_153821" in other vectors (3)
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CNY 3,990.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Pmx1; Prx-1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR214196 representing NM_153821
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCTCCAGCTACGGGCACGTTCTGGAGCGGCAACCGGCTCTGGGCGGCCGCTTGGATAGCCCTGGCA ACCTCGACACCCTGCAGGCGAAAAAGAACTTCTCCGTCAGTCACCTGCTAGACCTGGAGGAGGCCGGGGA CATGGTGGCGGCACAGGCGGACGAAAGCGTGGGCGAGGCGGGCCGGAGCCTGCTGGAGTCACCGGGACTG ACCAGTGGCAGCGACACCCCTCAGCAGGACAATGATCAGCTGAACTCTGAGGAGAAGAAGAAGAGAAAGC AACGGAGAAACAGGACAACCTTCAATAGCAGCCAGCTGCAGGCCTTGGAGCGTGTCTTCGAGAGGACCCA TTACCCGGATGCTTTTGTACGGGAAGATCTTGCACGTCGGGTGAACCTCACGGAGGCCAGAGTGCAGGTG TGGTTTCAGAACCGAAGAGCCAAGTTCCGCAGGAATGAGCGAGCCATGCTGGCCAATAAAAACGCTTCTC TCCTCAAGTCCTACTCAGGAGACGTGACTGCCGTGGAACAACCCATTGTACCTCGTCCTGCTCCCAGACC CACCGATTATCTCTCCTGGGGGACAGCCTCTCCGTACAGCGCCATGGCTACTTATTCTGCCACATGTGCC AACAATAGCCCTGCGCAGGGTATCAACATGGCCAACAGCATTGCCAACCTGAGACTGAAGGCCAAGGAAT ATAGTTTACAGAGGAACCAGGTGCCAACAGTCAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR214196 representing NM_153821
Red=Cloning site Green=Tags(s) MTSSYGHVLERQPALGGRLDSPGNLDTLQAKKNFSVSHLLDLEEAGDMVAAQADESVGEAGRSLLESPGL TSGSDTPQQDNDQLNSEEKKKRKQRRNRTTFNSSQLQALERVFERTHYPDAFVREDLARRVNLTEARVQV WFQNRRAKFRRNERAMLANKNASLLKSYSGDVTAVEQPIVPRPAPRPTDYLSWGTASPYSAMATYSATCA NNSPAQGINMANSIANLRLKAKEYSLQRNQVPTVN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_153821 |
ORF Size | 735 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_153821.1, NP_722543.1 |
RefSeq Size | 1375 bp |
RefSeq ORF | 738 bp |
Locus ID | 266813 |
UniProt ID | P63014 |
MW | 27.3 kDa |
Gene Summary | binds the collagen I alpha 1 and the osteocalcin promoters; acts as a negative regulator of transcription [RGD, Feb 2006] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
---|
Gatekeeper transcription factors regulate switch between lineage preservation and cell plasticity
,Ray, T;Shah, A;Bulla, GA;Ray, PS;,
bioRxiv
[PRRX1]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN214196 | Prrx1 (untagged ORF) - Rat paired related homeobox 1 (Prrx1), (10 ug) |
CNY 3,990.00 |
|
RR214196L3 | Lenti ORF clone of Prrx1 (Myc-DDK-tagged ORF) - Rat paired related homeobox 1 (Prrx1), (10 ug) |
CNY 6,080.00 |
|
RR214196L4 | Lenti ORF clone of Prrx1 (mGFP-tagged ORF) - Rat paired related homeobox 1 (Prrx1), (10 ug) |
CNY 6,650.00 |