Arhgef28 (NM_001108542) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Rgnef
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR214009 representing NM_001108542 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTGAGCTGCAGTGAAGTGCCTCTTTACGGACAGAGGACGGTCTATGCAAAGTTCGGCAAAAACG TTTATCTTCCTGAAGATGCGGAGTATTACTTTATTTATGATGGGTCGCATCAGAGGCACGTGGTGATTGC GGATCGTGTTGAGGATAATGTTCTCCAGTCTATCATTCCAGGCCATTGGCTTCAAGAGACGGTGATGGTA TCTGTGTGCCTCTGCTCAGAAGGGTATTCCCCGGTGACCATGGGCTCTGGCTCAGTGACCTATGTGGACA ACATGGCCTGCAGGCTGGCTCGTCTGCTGGTCACTCAGGCTGACCGACTCACGCCCTGTAGTCACCAGAC ACTGCTGACCCCGTTTGCCCTGACAGCAGAAGCGCTGCCTGCCTTGGATGAGGAACTCGTGCTGGCTCTG ACCCAACTGGAGCTGCCTTTGGGTTGGACAGTCCTGGGAAATTCCTCACTTGAAGTGTGTCTACACAGAG AGTCTCTCCTGCACCTGGCAGTGAGGTGGGCCCTGCCTAAGCTATTCCATTTCCTCTTGTGCCTCCCTGG AGGAGTCAAGGCCTTGGAGCTACCTAATGAAGAGGCCACCACACCATTAGACTTAGCCTTGCATGGCGGA CACTCTATGCTGGTGGAAGACATCTCAAATTTTCAGGGTCGCCGCTCCCCAGGCTTCTCCCGACTGCGTC TTAATGAAGATGCCACGCTGCAGTTCGATCGGTCATCAGAAACGTTGACGCTGACCGTTAACCACACAGC TGAGCATCTGTTGGAAGCAGATATCAAACTCTTCCGGAAGTATTTCTGGGACAGAGCCTTTCTTGTCAAG GCTCTTGATCAAGAAGCCAAGACAGAGAGAGCAACCATGCCTTCTGGAGCTGCGGAAACTGAAGAAGAGG TTAGGAATCTGGAGTCTGGCAGATCACCCTCAGAAGAGGAGGAGGATGGACAGCTCGTCAAAAGCCAGGC CGACGGGCCGAGTGAACAGGAAGACCAGGACCGCCTAGTTTTGGATCACTCCTTTGATGGCCTCAAAAAG TCCAAGCACGCCCCAGCGTCCCTTGCTGCAGGCCAGCTCTCTGACGTGCCGAATGGGGGTGACGAAGTCT ACGCTAACTGTATGGTGATTGATCAGGTTGGCGATTTGGACATCAACTATATAAACATAGAGGGACTCTC CACCCATACTAGCCCGGAGTCCATGAGGTCCACTCTGGGGCCTCAGGCTTGTAAGCACATCCTTCCTCCT GACACCAGTCCCTGCGGGCACCTGCTCTGTGAGGACAGTGACAGGACCCTGGATGCTGCAGCCTCTCAGT CCTGCATGTCACCACCCAGTTCTCACACTTCCAACTTGAACCTTTCTTTTGGTCTCCATGGATTTGTAAA GGAGCAGAGTCATCTCAAGAAAAGAAGTTCTAGCCTGGATGCCCTCGTTGCCGACAGTGAAGAGGAAGGG CGTTCTGAGCCCCCCATCTGCTATGCTGTCGGCTCTCAGAGTTCCCCCAGAACTGGGCTCCCCGGCGGAG ATGAACTGGATTCTTTTGATGCGAACACCGAACCAGACTGTAACATCTCTAGGACCGAATCACTTTCCCT GTCCAGCACTCTGCATTCGAAGGAGTCCCTGCTTTCTGGGATCCGATCACGCTCCTACTCCTGCTCATCA CCCAAAATCTCTTCAGGAAAATCCCGGCTAGTGCGCGATTTCACAGTGTGCAGTTCCAGTGAAGAACAGA GATCATATAGTTTCCAGGAGCCACCGGGAGAAAAAAGAATTCAGGAAGAAGAATGGGACGACTACATCAT TCCTGCCAAAGCAGAGTCCGAAAAGCACAAAGTGAGCCGGACTTTCAGCTTCCTCATGAACAGGATGACC AGTCCTCGGAACAAATCTAAGATGAAAAACAAGGACACTAAAGATAAAGAAAAACTCAATCGACATCAGT TTGTCCCGGGAACGTTCTCTGGAGTTCTGCAGTGTTCCGGTTGTGACAAAACACTCCTGGGGAAAGAGTC ACTCCAGTGTGCCAACTGCAAGGCCAACACACACAAGGGCTGCAAAGACACTGTGCCTCCGTGTACCAAG AAATTCCAAGACAAATACAACAAGAACAAACCACAGACCATCCTCGGAAGCTCTTCAGTTAGAGACGTCC CACCGCCTGGGCTCTCCTTGCACCCTTCCCCCTCCATGCCTATTGGACTGCCAGCTGGAAGGAAGGAAAC TGCAGGGCAGGCACATCCGTTGTCCAGAAGTGTCCCAGGCACCACTTTGGAAAGCTTCAGGAGGGCAGTG ACATCCTTGGAGTCTGAGGGTGACAGTAACAGCTGGAGAAGCAGGTCTCATTCCGATGAGCTGTTCCAGT CCATGGGCTCGTCTCCTTCCACGGAGTCATTCATGATGGAAGATGTCGTGGATTCTTCTCTGTGGAGTGA GCTCAGCAGTGATGCTCAAGAGTTTGAAGCTGAGTCCTGGAGTCTCGTGGTGGACCCTTCCTTCTGCAGC AGACAGGAGAAGGATGTCATCAAAAGACAGGATGTCATTTTTGAGCTGATGCAGACGGAGGTGCACCACA TCCAGACACTGCTCATCATGTCTGAAGTCTTCAGGAAGGGCATGAAGGAGGAGCTGCAGCTGGACCACAG CACGGTGGACAGGATCTTCCCCTGCTTAGATGAGCTGCTGGAGACTCACAAGCATTTCTTCTTCAGCATG AAGGAGCGCAGGCAGGAGTCCTGCACCGGCAATGACAGGAACTTTGTGATCAACCAAATCGGAGACATTT TGGTACAACAGTTTTCAGAAGAAAATGCAAGCAAGATGAAGAGGATCTATGGAGATTTCTGCAGCCATCA CAAAGAAGCCATGAGCCTCTTTAAAGAGCTCCAGCAGAACAAAAAGTTTCAGAATTTTATTAAGATCCGA AATAGCAACCTGTTGGCCCGTCGCCGTGGAATCCCAGAGTGCATCCTGCTTGTCACCCAGCGCATCACGA AGTACCCGGTCTTGGTGGAGAGGATCCTGCAGTACACCAAGGAAAGGACCGAAGAGCACAGAGACCTGTG TAGAGCTCTTGGCTTAATTAAAGACATGATCGCAGCCGTGGACCTGAAAGTCAGTGAATATGAGAAGAAC CAAAAATGGCTCGAGATCCTAAATAAAATCGAGAACAAAACATACACAAAGCTCAAAAATGGCCACGTGT TTAGAAAGCAGGCGCTGATGAGCCAAGAAAGGGCTCTTCTGCACGACGGGCTCGTTTACTGGAAAACTGC GACAGGCCGTTTCAAAGACATCCTGGCCCTGCTTCTCACCGATGTGCTGCTCTTCTTGCAAGAAAAAGAC CAGAAGTATATCTTTGCAGCTGTTGACCAGAAGCCTTCAGTTATTTCCCTTCAGAAGCTCATCGCTAGGG AAGTCGCCAACGAGGAGAGAGGCATGTTTCTCATCAGTGCTTCGTCAGCGGGTCCCGAGATGTATGAAAT CCACACCAGTTCCAAAGAGGAACGGAATAACTGGATGAGGCGGATCCAGCAGGCAGTAGAAAGTTGTCCA GAAGAAGAAGGAGGAAGGACAAGTGAGTCTGATGAAGAGAGGCGGAAAGCTGACGCTAGAGTGGCCAAAA TTCAGCAATGTCAAGAGATTCTCAGCAACCAAGACCAGCAGATCTGCACATATTTGGAAGAAAAGCTGCA CATCTACGCTGAGCTTGGGGAACTAAGTGGCTTTGAGGATGTCCACCTAGAACCCCATCTGCTCATCAAG CCCGACCCCGGAGAACCTCCCCAGGCAGCCTCCCTGCTTGCAGCAGCCCTGAGAGAAGCTGAGAGTCTGC AAGTTGCTGTGAAGGCTTCAAAGCTGGCTGATGTAAGCCAGCCATCTGAAGAAGGCCCTGGAGGGGCTGT CCTGGCTGACACACTCAGTGCACAAGATGCACCAGCATCGCCGACTGCCTTCACAGAAGGGACAGAAGGA AGAGGCTGTTGGGATGTGGATCCCCGGCTCCAGGGTGTGGTAACGGACTTGGCGGTCTCTGATGCAGGGG AGAAGGTGGAATATAGAAGTTTTTCCGGTTCTTCACAATCAGAGATTATACAAGCGATACAGAACTTAAC GCGTCTCCTCTACAGCCTTCAGGCTGCCTTGACCATCCAGGACAGCCACATTGAGATCCACAAGCTAGTT CTACAGCAGCAGGAGAGCTTGGCCCCTAGCCACTCCTTCCGAGGTGGCCCCTTGCAAGACCAGGAGAAAT CCCGTTACCTGGAAAAGCATCGTGAAGAGCTGGCCAACATCCACAAGCTCCAGTACCAGTTCCAACAAGA GCAGCGGCGTTGGCACCGCACATGTGACCAGCAACAGCGTGAGCAGGAGGCCCAAGAGAGCTGGCTGCAG GCACGGGAACGTGAGTGCCAGTCGCAGGAGGAATTACTGCTGCGCCACCGTAGCGAACTGGACCACCAAC TCCAGGAGTACCAGCAGAACCTCGAGCGGTTGCGGGAGGGCCAGAGGATGGTGGAGAGGGAGAGGCAGAG GATGCGTGACCAGCAGGGTCTGCTGGGCCACTGTAAGCATAGTCGCCAGAGGAGCCTCCCAGCCGTCTTC TCTCCGGGGAGCAAAGAGGTAACGGAACTTAATCGAGCTGAGAGTTTGTGTCATGAAAAATCATTTTTCC TCAATGACGCCTTCACACACATGTCGCTTAACACATCCAATAAACCAAACCCATCAGGTGCCCCCTGGGA CGCTCACCCCCCAGGTGGGTCTCATCTTGATTTGGCAAGGACTAGTGATAGTCAGATAGAAGTGAAGATG GACGTTTCTCAGCCTTCCGATGTCAACTCTGAACTCTGGACAACAGGGCTGGGCCCTCAGAGACCTGCTC GCCAAGAAAGCATCAAGGATTCTTGTAAAAATGTTGCAGATTTGAACTCCTTCCAGACTGAATCCCCAGA TCCCCAGGACTCAAATCAACGTGGCCCTCAGCCACAGACACTCATAACAGAAGCAAAACTAACTCCCCCG ATGGCGGCCGGACATGGTGGAGATGCTGGGGATGGAGCTGAAGAGAACATTGTTTACCTC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR214009 representing NM_001108542 Red=Cloning site Green=Tags(s) MELSCSEVPLYGQRTVYAKFGKNVYLPEDAEYYFIYDGSHQRHVVIADRVEDNVLQSIIPGHWLQETVMV SVCLCSEGYSPVTMGSGSVTYVDNMACRLARLLVTQADRLTPCSHQTLLTPFALTAEALPALDEELVLAL TQLELPLGWTVLGNSSLEVCLHRESLLHLAVRWALPKLFHFLLCLPGGVKALELPNEEATTPLDLALHGG HSMLVEDISNFQGRRSPGFSRLRLNEDATLQFDRSSETLTLTVNHTAEHLLEADIKLFRKYFWDRAFLVK ALDQEAKTERATMPSGAAETEEEVRNLESGRSPSEEEEDGQLVKSQADGPSEQEDQDRLVLDHSFDGLKK SKHAPASLAAGQLSDVPNGGDEVYANCMVIDQVGDLDINYINIEGLSTHTSPESMRSTLGPQACKHILPP DTSPCGHLLCEDSDRTLDAAASQSCMSPPSSHTSNLNLSFGLHGFVKEQSHLKKRSSSLDALVADSEEEG RSEPPICYAVGSQSSPRTGLPGGDELDSFDANTEPDCNISRTESLSLSSTLHSKESLLSGIRSRSYSCSS PKISSGKSRLVRDFTVCSSSEEQRSYSFQEPPGEKRIQEEEWDDYIIPAKAESEKHKVSRTFSFLMNRMT SPRNKSKMKNKDTKDKEKLNRHQFVPGTFSGVLQCSGCDKTLLGKESLQCANCKANTHKGCKDTVPPCTK KFQDKYNKNKPQTILGSSSVRDVPPPGLSLHPSPSMPIGLPAGRKETAGQAHPLSRSVPGTTLESFRRAV TSLESEGDSNSWRSRSHSDELFQSMGSSPSTESFMMEDVVDSSLWSELSSDAQEFEAESWSLVVDPSFCS RQEKDVIKRQDVIFELMQTEVHHIQTLLIMSEVFRKGMKEELQLDHSTVDRIFPCLDELLETHKHFFFSM KERRQESCTGNDRNFVINQIGDILVQQFSEENASKMKRIYGDFCSHHKEAMSLFKELQQNKKFQNFIKIR NSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKERTEEHRDLCRALGLIKDMIAAVDLKVSEYEKN QKWLEILNKIENKTYTKLKNGHVFRKQALMSQERALLHDGLVYWKTATGRFKDILALLLTDVLLFLQEKD QKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTSSKEERNNWMRRIQQAVESCP EEEGGRTSESDEERRKADARVAKIQQCQEILSNQDQQICTYLEEKLHIYAELGELSGFEDVHLEPHLLIK PDPGEPPQAASLLAAALREAESLQVAVKASKLADVSQPSEEGPGGAVLADTLSAQDAPASPTAFTEGTEG RGCWDVDPRLQGVVTDLAVSDAGEKVEYRSFSGSSQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHKLV LQQQESLAPSHSFRGGPLQDQEKSRYLEKHREELANIHKLQYQFQQEQRRWHRTCDQQQREQEAQESWLQ ARERECQSQEELLLRHRSELDHQLQEYQQNLERLREGQRMVERERQRMRDQQGLLGHCKHSRQRSLPAVF SPGSKEVTELNRAESLCHEKSFFLNDAFTHMSLNTSNKPNPSGAPWDAHPPGGSHLDLARTSDSQIEVKM DVSQPSDVNSELWTTGLGPQRPARQESIKDSCKNVADLNSFQTESPDPQDSNQRGPQPQTLITEAKLTPP MAAGHGGDAGDGAEENIVYL SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001108542
ORF Size	5100 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001108542.1, NP_001102012.1
RefSeq Size	6195 bp
RefSeq ORF	5103 bp
Locus ID	361882
MW	190.8 kDa
Gene Summary	Functions as a RHOA-specific guanine nucleotide exchange factor regulating signaling pathways downstream of integrins and growth factor receptors. Functions in axonal branching, synapse formation and dendritic morphogenesis. Functions also in focal adhesion formation, cell motility and B-lymphocytes activation. May regulate NEFL expression and aggregation and play a role in apoptosis.[UniProtKB/Swiss-Prot Function]

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