Glrx2 (NM_001013034) Rat Tagged ORF Clone
CAT#: RR214006
- TrueORF®
Glrx2 (Myc-DDK-tagged ORF) - Rat glutaredoxin 2 (Glrx2), (10 ug)
"NM_001013034" in other vectors (3)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR214006 representing NM_001013034
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAAACAGCACATCCTCGTTTTGGGGGAAGTCAGCCACTACTCCTGTGAACCAGATCCAAGAAACAA TTTCTAATAATTGTGTGGTGATTTTCTCAAAATCATCCTGCTCTTACTGTTCAATGGCCAAGAAGATTTT CCATGACATGAATGTCAACTATAAAGTCGTGGAGTTGGATATGGTGGAATATGGTAGCCAGTTTCAAGAG GCTCTTTACAAGATGACTGGAGAAAGAACTGTTCCCAGGATATTTGTGAATGGAATATTTATCGGAGGTG CGGCCGACACTCACAGGCTTCACAAAGAAGGGAAATTGCTGCCTCTGGTTCACCAGTGCTATTTAAACAA AAGCAAGAGGAAAGACGTCGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR214006 representing NM_001013034
Red=Cloning site Green=Tags(s) MGNSTSSFWGKSATTPVNQIQETISNNCVVIFSKSSCSYCSMAKKIFHDMNVNYKVVELDMVEYGSQFQE ALYKMTGERTVPRIFVNGIFIGGAADTHRLHKEGKLLPLVHQCYLNKSKRKDVE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001013034 |
ORF Size | 372 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001013034.1, NP_001013052.1 |
RefSeq Size | 1432 bp |
RefSeq ORF | 375 bp |
Locus ID | 114022 |
UniProt ID | Q6AXW1 |
MW | 14 kDa |
Gene Summary | Glutathione-dependent oxidoreductase that facilitates the maintenance of mitochondrial redox homeostasis upon induction of apoptosis by oxidative stress. Involved in response to hydrogen peroxide and regulation of apoptosis caused by oxidative stress. Acts as a very efficient catalyst of monothiol reactions because of its high affinity for protein glutathione-mixed disulfides. Can receive electrons not only from glutathione (GSH), but also from thioredoxin reductase supporting both monothiol and dithiol reactions. Efficiently catalyzes both glutathionylation and deglutathionylation of mitochondrial complex I, which in turn regulates the superoxide production by the complex. Overexpression decreases the susceptibility to apoptosis and prevents loss of cardiolipin and cytochrome c release (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN214006 | Glrx2 (untagged ORF) - Rat glutaredoxin 2 (Glrx2), (10 ug) |
CNY 3,990.00 |
|
RR214006L3 | Lenti ORF clone of Glrx2 (Myc-DDK-tagged ORF) - Rat glutaredoxin 2 (Glrx2), (10 ug) |
CNY 6,080.00 |
|
RR214006L4 | Lenti ORF clone of Glrx2 (mGFP-tagged ORF) - Rat glutaredoxin 2 (Glrx2), (10 ug) |
CNY 6,650.00 |