Hipk2 (NM_001108622) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR213998 representing NM_001108622 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCCCGTGTACGAAGGTATGGCCTCACATGTGCAAGTTTTCTCCCCTCACACCCTTCAATCAAGTG CCTTCTGTAGTGTGAAGAAACTAAAAGTAGAGCCAAGTTCCAACTGGGACATGACTGGGTACGGCTCCCA CAGCAAAGTGTACAGCCAGAGCAAGAACATACCACCTTCTCAGCCAGCCTCCACAACCGTCAGCACCTCC TTGCCAATCCCAAACCCAAGCCTACCTTACGAGCAGACCATCATCTTCCCAGGAAGTACTGGACACATAG TTGTAACATCAGCAAGTAGCACTTCTGTCACCGGGCAAGTCCTCGGTGGACCACATAACCTAATGCGTCG AAGCACTGTGAGCCTCCTTGACACCTACCAAAAATGTGGACTCAAGCGCAAGAGTGAGGAGATTGAGAAC ACAAGCAGCGTGCAGATCATTGAGGAGCATCCACCCATGATTCAGAATAATGCCAGCGGGGCCACTGTCG CCACTGCCACCACATCCACTGCCACCTCCAAAAACAGTGGCTCCAACAGTGAAGGTGACTATCAGCTGGT GCAGCATGAAGTGCTGTGCTCTATGACAAACACCTATGAGGTTTTAGAGTTCTTGGGGCGAGGGACATTT GGGCAAGTGGTCAAGTGCTGGAAACGGGGCACCAATGAAATTGTGGCCATTAAGATCCTAAAGAACCACC CATCCTATGCCCGGCAAGGCCAGATTGAAGTGAGCATCCTGGCCCGGCTGAGCACGGAGAGCGCTGATGA CTACAATTTTGTGCGGGCCTATGAGTGCTTCCAGCACAAGAATCACACATGCTTAGTCTTTGAGATGTTG GAGCAGAACCTCTATGACTTTCTGAAACAGAACAAGTTTAGTCCCTTGCCCCTCAAGTATATTCGCCCAG TCCTCCAGCAGGTAGCCACAGCCCTGATGAAACTCAAAAGCCTGGGTCTTATCCATGCTGACCTCAAACC AGAAAACATCATGCTGGTGGATCCATCCAGACAACCATACCGAGTGAAGGTCATTGACTTTGGTTCAGCT AGTCATGTGTCCAAAGCTGTCTGTTCTACGTACTTGCAATCCAGATACTACAGGGCCCCTGAAATCATCC TTGGTTTGCCATTCTGTGAGGCAATTGACATGTGGTCCCTGGGTTGTGTCATTGCTGAGCTGTTCCTGGG CTGGCCATTATACCCAGGTGCTTCTGAGTATGATCAGATTCGGTATATTTCACAAACACAGGGTTTACCA GCTGAATATTTATTAAGTGCAGGGACAAAGACAACTAGGTTTTTCAACCGTGACACAGACTCACCGTATC CTTTGTGGAGGCTAAAGACACCAGATGACCATGAAGCAGAGACGGGGATTAAATCAAAAGAAGCAAGAAA GTACATTTTCAACTGTTTGGATGACATGGCCCAGGTGAACATGACAACAGATTTGGAAGGGAGTGACATG TTAGTAGAGAAGGCAGACCGGCGGGAGTTCATTGACCTGTTAAAGAAGATGCTGACCATAGATGCTGATA AGAGGATCACTCCCATTGAAACTCTGAACCACCCCTTTGTCACCATGACGCACCTACTGGATTTTCCTCA TAGTACTCATGTCAAATCTTGTTTCCAAAACATGGAGATTTGCAAGCGCCGGGTGAATATGTATGACACA GTGAACCAGAGCAAAACACCTTTCATCACACATGTGGCCCCCAGCACATCTACCAACTTGACCATGACCT TTAACAACCAGCTGACCACTGTCCACAACCAGGCTCCCACCTCCACCAGTGCCACTCTTTCCTTAGCCAA TCCCGAAGTCTCCATACTAAACTACCAATCTACACTCTACCAGCCCTCAGCGGCATCCATGGCCGCAGTG GCCCAGCGGAGCATGCCCCTGCAGACGGGAACAGCCCAAATTTGTGCCCGGCCTGACCCTTTCCAGCAAG CTCTCATCGTGTGTCCCCCTGGCTTCCAAGGCCTGCAGGCCTCTCCCTCCAAGCACGCTGGCTACTCAGT GCGGATGGAAAATGCTGTTCCCATCGTCACTCAGGCTCCGGGCGCTCAGCCTCTTCAGATCCAGCCAGGT CTGCTTGCTCAGGCCTGGCCAGGTGGGGCCCAACAGATCCTCCTTCCTCCAGCATGGCAGCAGCTCACTG GCGTGGCTACCCACACATCCGTACAGCACGCAGCTGTGATTCCCGAGACCATGGCAGGCACTCAGCAACT GGCTGACTGGAGAAACACACATGCTCACGGAAGCCATTACAATCCCATCATGCAGCAGCCTGCACTCTTG ACTGGTCATGTGACCCTTCCAGCTGCCCAGCCCTTAAATGTGGGTGTAGCCCACGTGATGAGACAGCAAC CAACCAGCACCACCTCCTCCAGGAAGAGTAAGCAGCACCAATCATCAGCGAGAAATGTCTCCACCTGTGA AGTGACCTCTTCCCAGGCTATCAGCTCCCCTCAGCGATCCAAGCGTGTCAAGGAGAACACTCCCCCGAGG TGTGCAATGGTACACAGCAGCCCAGCTTGCAGCACCTCACTCACCTGTGGGTGGGGTGACGGGGCCTCCA GCACCACCCGGGACCGACAGCGGCAGACCATTGTCATCCCCGACACCCCGAGCCCCACAGTCAGTGTCAT CACCATCAGCAGCGACACAGATGAGGAGGAGGAACAGAAGCACGCCCCCACCAGCACAGTCTCCAAGCAA AGAAAAAATGTCATCAGCTGTGTCACAGTCCATGATTCTCCCTACTCTGACTCCTCCAGCAACACCAGCC CCTACTCGGTGCAGCAGCGCACAGGGCACAACGGCACCAACACCTTGGACACCAAGGGGGGCCTAGAGAA CCACTGCACCGGCAACCCCCGCACCATCATCGTACCCCCACTGAAGACGCAGGCCAGTGAAGTGTTGGTA GAATGTGACAGCCTAGGGCCAGCAATCAGTACCGGTCACCATTCATCCTCCTTCAAGTCCAAGTCCTCCA GCACCGTGACCTCCACCAGTGGCCACTCTTCAGGGAGCTCGTCAGGAGCCATCGCCTACCGTCAACAGCG GCCAGGCCCCCACTTCCAGCAGCAGCAGCCCCTCAATCTCAGCCAGGCTCAGCAGCACATGGCTGCAGAC CGCACTGGGAGTCACCGTCGGCAGCAGGCCTACATCACTCCTACCATGGCTCAGGCTCCGTACACCTTCC CGCACAGCAGCCCCAGCCATGGCACTGTTCACCCCCACCTGGCTGCGGCTGCCCACCTCCCTGCCCAGCC TCACCTCTACACCTACACAGCGCCCACAGCCCTAGGCTCCACCAGCACTGTAGCCCACCTGGTGGCATCC CAAGGCTCAGCACGACACACCGTGCAGCACACTGCCTATCCGGCCAGCATCGTCCACCAGGTCCCCGTGA GCATGGGGCCCCGGGTCCTGCCCTCGCCCACCATTCACCCCAGTCAGTATCCGGCCCAGTTTGCCCACCA GACCTATATCAGCGCCTCGCCAGCCTCCACCGTCTACACTGGATATCCACTGAGTCCTGCCAAGGTCAAC CAATACCCTTACATA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR213998 representing NM_001108622 Red=Cloning site Green=Tags(s) MAPVYEGMASHVQVFSPHTLQSSAFCSVKKLKVEPSSNWDMTGYGSHSKVYSQSKNIPPSQPASTTVSTS LPIPNPSLPYEQTIIFPGSTGHIVVTSASSTSVTGQVLGGPHNLMRRSTVSLLDTYQKCGLKRKSEEIEN TSSVQIIEEHPPMIQNNASGATVATATTSTATSKNSGSNSEGDYQLVQHEVLCSMTNTYEVLEFLGRGTF GQVVKCWKRGTNEIVAIKILKNHPSYARQGQIEVSILARLSTESADDYNFVRAYECFQHKNHTCLVFEML EQNLYDFLKQNKFSPLPLKYIRPVLQQVATALMKLKSLGLIHADLKPENIMLVDPSRQPYRVKVIDFGSA SHVSKAVCSTYLQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGASEYDQIRYISQTQGLP AEYLLSAGTKTTRFFNRDTDSPYPLWRLKTPDDHEAETGIKSKEARKYIFNCLDDMAQVNMTTDLEGSDM LVEKADRREFIDLLKKMLTIDADKRITPIETLNHPFVTMTHLLDFPHSTHVKSCFQNMEICKRRVNMYDT VNQSKTPFITHVAPSTSTNLTMTFNNQLTTVHNQAPTSTSATLSLANPEVSILNYQSTLYQPSAASMAAV AQRSMPLQTGTAQICARPDPFQQALIVCPPGFQGLQASPSKHAGYSVRMENAVPIVTQAPGAQPLQIQPG LLAQAWPGGAQQILLPPAWQQLTGVATHTSVQHAAVIPETMAGTQQLADWRNTHAHGSHYNPIMQQPALL TGHVTLPAAQPLNVGVAHVMRQQPTSTTSSRKSKQHQSSARNVSTCEVTSSQAISSPQRSKRVKENTPPR CAMVHSSPACSTSLTCGWGDGASSTTRDRQRQTIVIPDTPSPTVSVITISSDTDEEEEQKHAPTSTVSKQ RKNVISCVTVHDSPYSDSSSNTSPYSVQQRTGHNGTNTLDTKGGLENHCTGNPRTIIVPPLKTQASEVLV ECDSLGPAISTGHHSSSFKSKSSSTVTSTSGHSSGSSSGAIAYRQQRPGPHFQQQQPLNLSQAQQHMAAD RTGSHRRQQAYITPTMAQAPYTFPHSSPSHGTVHPHLAAAAHLPAQPHLYTYTAPTALGSTSTVAHLVAS QGSARHTVQHTAYPASIVHQVPVSMGPRVLPSPTIHPSQYPAQFAHQTYISASPASTVYTGYPLSPAKVN QYPYI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001108622
ORF Size	3585 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001108622.1, NP_001102092.1
RefSeq Size	4851 bp
RefSeq ORF	3588 bp
Locus ID	362342
MW	130.4 kDa

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