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Hcn1 (NM_053375) Rat Tagged ORF Clone
CAT＃: RR213992
- TrueORF®
Hcn1 (Myc-DDK-tagged ORF) - Rat hyperpolarization-activated cyclic nucleotide-gated potassium channel 1 (Hcn1), (10 ug)
"NM_053375" in other vectors (3)
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CNY 11,780.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR213992 representing NM_053375
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGGCGGCGGCAAGCCCAACTCCGCTTCCAACAGCCGCGACGATGGCAACAGCGTCTACCCCTCCA AGGCGCCCGCGACGGGGCCGGCGGCGGCCGACAAGCGCCTGGGGACCCCGCCGGGGGGCGGCGCGGCCGG GAAGGAACACGGCAACTCCGTGTGCTTCAAGGTGGACGGCGGCGGAGGAGAGGAGCCGGCGGGCAGCTTC GAGGATGCCGAGGGGCCCCGGCGACAGTACGGTTTCATGCAGAGGCAGTTCACCTCCATGCTGCAGCCTG GGGTCAACAAATTCTCCCTCCGCATGTTCGGGAGCCAGAAGGCGGTGGAGAAGGAGCAGGAAAGGGTTAA AACTGCAGGCTTCTGGATTATCCATCCGTACAGTGACTTCAGGTTTTATTGGGATTTAATAATGCTTATA ATGATGGTTGGAAATTTGGTCATCATACCAGTTGGAATCACATTCTTCACAGAGCAAACAACAACACCGT GGATTATTTTCAATGTGGCATCAGATACAGTTTTCCTGTTGGACCTAATCATGAATTTTAGGACTGGGAC TGTCAACGAAGACAGCTCTGAAATCATCCTGGACCCTAAAGTAATCAAGATGAATTATTTAAAAAGCTGG TTCGTGGTGGACTTCATCTCCTCGATCCCGGTGGATTATATCTTTCTTATTGTAGAGAAAGGAATGGATT CGGAAGTTTACAAGACCGCCAGAGCACTTCGGATCGTGAGGTTTACAAAAATTCTCAGTCTCTTGCGTTT ATTACGCCTTTCAAGGTTAATTAGATACATACACCAGTGGGAAGAGATATTCCACATGACATATGATCTC GCCAGTGCAGTGGTGAGAATCTTCAACCTCATTGGCATGATGCTGCTCCTGTGTCACTGGGATGGCTGTC TTCAGTTTCTGGTCCCCCTGCTGCAGGACTTCCCACCGGATTGCTGGGTTTCTCTAAATGAAATGGTTAA TGATTCATGGGGGAAACAGTATTCCTACGCACTCTTCAAAGCTATGAGTCACATGCTGTGCATTGGTTAT GGCGCCCAGGCCCCCGTCAGCATGTCTGACCTCTGGATTACCATGCTGAGCATGATTGTTGGGGCCACCT GCTATGCCATGTTTGTCGGCCATGCCACAGCTTTGATCCAGTCTCTGGATTCTTCAAGGAGGCAGTATCA AGAGAAGTACAAGCAAGTAGAGCAATACATGTCATTCCACAAGTTACCAGCTGACATGCGCCAGAAGATA CATGATTACTATGAGCACCGATACCAAGGCAAGATCTTCGATGAGGAAAATATTCTCAGTGAACTTAATG ATCCTCTGAGAGAGGAAATAGTCAACTTCAACTGCCGGAAACTGGTGGCCACCATGCCTCTCTTTGCTAA CGCGGATCCCAATTTCGTGACGGCCATGCTGAGCAAGCTGAGATTTGAGGTGTTCCAGCCCGGAGACTAT ATCATTCGAGAAGGAGCTGTGGGGAAGAAAATGTATTTCATCCAGCATGGTGTGGCTGGTGTCATCACCA AGTCCAGTAAAGAAATGAAGTTGACAGACGGCTCTTACTTTGGAGAAATATGCCTGCTGACCAAGGGCCG GCGCACTGCCAGTGTTCGAGCTGATACATACTGTCGCCTTTACTCCCTTTCGGTGGACAATTTCAACGAG GTCTTGGAGGAATATCCAATGATGAGAAGAGCCTTTGAGACAGTTGCTATTGACCGACTAGATCGGATAG GCAAGAAAAACTCTATTCTCCTGCAGAAGTTCCAGAAGGATCTGAACACTGGTGTTTTCAACAACCAGGA GAATGAGATCCTGAAGCAGATTGTGAAGCATGACAGAGAGATGGTACAAGCGATCCCTCCAATCAACTAT CCTCAAATGACAGCCCTGAATTGCACATCTTCAACCACCACCCCAACGTCGCGCATGAGGACCCAATCTC CACCAGTCTACACAGCGACCAGCCTCTCTCACAGCAACCTGCACTCACCCAGCCCCAGCACACAGACGCC TCAACCCTCAGCCATCCTTTCACCCTGCTCCTACACCACAGCAGTCTGCAGTCCTCCTATACAGAGCCCC CTGGCCACGCGAACTTTCCATTATGCCTCTCCCACTGCATCCCAATTGTCACTCATGCAGCAGCCTCAGC CGCAGCTACAGCAATCCCAGGTACAGCAGACTCAGACTCAGACTCAGCAGCAGCAGCAGCAACAGCAGCC GCAGCCGCAGCCGCAGCAGCCGCAACAGCAACAACAGCAGCAACAGCAGCAGCAGCAGCAGCAGCAACAA CAGCAGCAGCAACAGCCACAGACACCTGGTAGTTCCACACCGAAAAATGAAGTGCACAAGAGCACTCAAG CTCTTCATAACACCCACCTGACCAGAGAAGTCAGGCCCCTCTCTGCCTCGCAGCCTTCGCTGCCCCATGA GGTCTCCACTATGATCTCCAGACCGCATCCCACTGTGGGCGAGTCCCTGGCTTCCATCCCTCAACCCGTG GCAACAGTCCACAGCACTGGCCTTCAGGCAGGGAGCAGGAGCACCGTGCCACAGCGTGTCACCTTGTTCA GACAGATGTCCTCGGGAGCCATTCCCCCCAACCGAGGAGTGCCTCCAGCACCCCCGCCACCAGCAGCTGT GCAGAGAGAGTCTCCCTCAGTCTTAAATAAAGACCCAGATGCAGAAAAACCCCGTTTTGCTTCGAATTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR213992 representing NM_053375
Red=Cloning site Green=Tags(s) MEGGGKPNSASNSRDDGNSVYPSKAPATGPAAADKRLGTPPGGGAAGKEHGNSVCFKVDGGGGEEPAGSF EDAEGPRRQYGFMQRQFTSMLQPGVNKFSLRMFGSQKAVEKEQERVKTAGFWIIHPYSDFRFYWDLIMLI MMVGNLVIIPVGITFFTEQTTTPWIIFNVASDTVFLLDLIMNFRTGTVNEDSSEIILDPKVIKMNYLKSW FVVDFISSIPVDYIFLIVEKGMDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDL ASAVVRIFNLIGMMLLLCHWDGCLQFLVPLLQDFPPDCWVSLNEMVNDSWGKQYSYALFKAMSHMLCIGY GAQAPVSMSDLWITMLSMIVGATCYAMFVGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPADMRQKI HDYYEHRYQGKIFDEENILSELNDPLREEIVNFNCRKLVATMPLFANADPNFVTAMLSKLRFEVFQPGDY IIREGAVGKKMYFIQHGVAGVITKSSKEMKLTDGSYFGEICLLTKGRRTASVRADTYCRLYSLSVDNFNE VLEEYPMMRRAFETVAIDRLDRIGKKNSILLQKFQKDLNTGVFNNQENEILKQIVKHDREMVQAIPPINY PQMTALNCTSSTTTPTSRMRTQSPPVYTATSLSHSNLHSPSPSTQTPQPSAILSPCSYTTAVCSPPIQSP LATRTFHYASPTASQLSLMQQPQPQLQQSQVQQTQTQTQQQQQQQQPQPQPQQPQQQQQQQQQQQQQQQQ QQQQQPQTPGSSTPKNEVHKSTQALHNTHLTREVRPLSASQPSLPHEVSTMISRPHPTVGESLASIPQPV ATVHSTGLQAGSRSTVPQRVTLFRQMSSGAIPPNRGVPPAPPPPAAVQRESPSVLNKDPDAEKPRFASNL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_053375 |
| ORF Size | 2730 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_053375.1, NP_445827.1 |
| RefSeq Size | 2807 bp |
| RefSeq ORF | 2733 bp |
| Locus ID | 84390 |
| UniProt ID | Q9JKB0 |
| MW | 102.4 kDa |
| Gene Summary | may generate neuronal pacemaker activity [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN213992 | Hcn1 (untagged ORF) - Rat hyperpolarization-activated cyclic nucleotide-gated potassium channel 1 (Hcn1), (10 ug) |
CNY 11,780.00 |
|
| RR213992L3 | Lenti ORF clone of Hcn1 (Myc-DDK-tagged ORF) - Rat hyperpolarization-activated cyclic nucleotide-gated potassium channel 1 (Hcn1), (10 ug) |
CNY 13,400.00 |
|
| RR213992L4 | Lenti ORF clone of Hcn1 (mGFP-tagged ORF) - Rat hyperpolarization-activated cyclic nucleotide-gated potassium channel 1 (Hcn1), (10 ug) |
CNY 14,820.00 |
