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Fzd8 (NM_001044251) Rat Tagged ORF Clone
CAT＃: RR213682
- TrueORF®
Fzd8 (Myc-DDK-tagged ORF) - Rat frizzled homolog 8 (Drosophila) (Fzd8), (10 ug)
"NM_001044251" in other vectors (3)
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CNY 8,840.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC112790 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR213682 representing NM_001044251
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTGGGGTTACCTGTTGGAAGTGACCTCGCTACTAGCCGCCTTGGCGGTGCTACAGCGCTCGAGCG GCGCTGCCGCGGCTTCGGCCAAGGAGCTGGCGTGCCAAGAGATCACAGTGCCGCTGTGCAAGGGCATCGG TTACAACTACACCTACATGCCCAACCAGTTCAACCACGACACGCAAGATGAGGCGGGCCTAGAGGTACAC CAGTTCTGGCCGCTGGTGGAGATCCAGTGCTCCCCGGACCTCAAGTTCTTTCTGTGTAGCATGTACACGC CCATCTGCCTGGAGGACTACAAGAAACCTCTGCCGCCTTGCCGCTCTGTGTGTGAACGCGCCAAGGCCGG CTGCGCGCCGCTCATGCGCCAGTACGGCTTTGCCTGGCCTGACCGAATGCGCTGCGATCGGCTGCCGGAG CAGGGCAACCCCGACACTCTGTGCATGGACTACAACCGCACCGACCTCACCACGGCCGCGCCCAGCCCAC CGCGCCGCCTGCCGCCGCCGCAGCCTGGCGAGCAGCCGCCCTCCGGCAGCGGCCACAGCCGCCCGCCAGG GGCCAGGCCCCCACATCGTGGCGGCAGCAGTAGGGGCAGCGGGGACACGGCGGCTGCGCCCCCCTCTCGC GGCGGGAAGGCGAGGCCCCCTGGTGGCGGCGCCGCTCCCTGCGAGCCCGGGTGCCAGTGCCGCGCGCCCA TGGTGAGTGTGTCCAGCGAACGCCACCCGCTCTACAACCGCGTCAAGACCGGCCAGATCGCCAACTGTGC GCTGCCCTGCCACAACCCCTTCTTTAGCCAGGATGAGCGCGCCTTCACTGTCTTCTGGATCGGCTTGTGG TCGGTGCTCTGCTTCGTCTCCACCTTCGCCACTGTCTCTACCTTCCTCATCGATATGGAGCGCTTTAAGT ACCCGGAACGGCCCATTATATTCCTCTCTGCCTGCTACCTCTTCGTGTCTGTCGGGTACCTGGTGCGCCT GGTGGCTGGACACGAGAAAGTGGCCTGCAGCGGCGGCGCGCCGGGTGCAGGAGGAGCTGGGGGTGCTGGC GGCGCGGCGACCGCTGGCGCGGGGGCGGCGGGAGCGGGGGCGAGCAGCCCGGGCGCGCGCGGCGAGTACG AGGAGCTGGGTGCAGTGGAGCAGCACGTGCGCTATGAGACCACTGGCCCCGCGCTGTGCACGGTAGTCTT CCTCCTTGTCTACTTTTTTGGCATGGCTAGCTCCATCTGGTGGGTAATCCTGTCTCTCACGTGGTTCTTG GCGGCTGGTATGAAATGGGGCAACGAGGCCATAGCAGGCTACTCGCAATACTTCCACCTTGCCGCGTGGC TCGTGCCCAGCGTCAAGTCCATAGCGGTGCTGGCGCTCAGCTCCGTGGACGGCGACCCGGTGGCGGGCAT CTGCTACGTGGGCAACCAGAGCCTTGACAACCTACGCGGCTTTGTACTGGCGCCACTGGTCATCTACCTC TTCATTGGGACTATGTTTCTGTTAGCTGGCTTCGTGTCGCTGTTCCGAATCCGCTCAGTCATCAAGCAAC AAGGAGGCCCAACCAAGACTCACAAGCTAGAAAAGCTCATGATCCGCTTGGGCCTCTTCACCGTGCTCTA CACGGTGCCCGCCGCCGTCGTTGTCGCCTGCCTTTTCTATGAGCAGCACAACCGACCGCGCTGGGAGGCC ACGCACAACTGCCCATGCCTTCGCGACCTGCAGCCAGACCAGGCTCGCAGGCCCGACTACGCGGTCTTCA TGCTCAAGTACTTCATGTGCCTAGTGGTGGGCATCACATCAGGCGTGTGGGTCTGGTCCGGCAAGACTCT GGAGTCCTGGCGTGCGTTGTGTACTCGCTGCTGCTGGGCCAGCAAGGGCGCTGCAGTTGGCGCGGGCGCC GGAGGCAGCGGCCCTGGGGGCGGTGGCCCCGGGCCCGGTGGGGGTGGGGGGCACGGCGGAGGTGGGGGAT CCCTCTACAGCGACGTCAGCACCGGCCTGACGTGGCGGTCTGGCACGGCCAGCTCTGTATCTTACCCTAA GCAAATGCCATTGTCCCAAGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR213682 representing NM_001044251
Red=Cloning site Green=Tags(s) MEWGYLLEVTSLLAALAVLQRSSGAAAASAKELACQEITVPLCKGIGYNYTYMPNQFNHDTQDEAGLEVH QFWPLVEIQCSPDLKFFLCSMYTPICLEDYKKPLPPCRSVCERAKAGCAPLMRQYGFAWPDRMRCDRLPE QGNPDTLCMDYNRTDLTTAAPSPPRRLPPPQPGEQPPSGSGHSRPPGARPPHRGGSSRGSGDTAAAPPSR GGKARPPGGGAAPCEPGCQCRAPMVSVSSERHPLYNRVKTGQIANCALPCHNPFFSQDERAFTVFWIGLW SVLCFVSTFATVSTFLIDMERFKYPERPIIFLSACYLFVSVGYLVRLVAGHEKVACSGGAPGAGGAGGAG GAATAGAGAAGAGASSPGARGEYEELGAVEQHVRYETTGPALCTVVFLLVYFFGMASSIWWVILSLTWFL AAGMKWGNEAIAGYSQYFHLAAWLVPSVKSIAVLALSSVDGDPVAGICYVGNQSLDNLRGFVLAPLVIYL FIGTMFLLAGFVSLFRIRSVIKQQGGPTKTHKLEKLMIRLGLFTVLYTVPAAVVVACLFYEQHNRPRWEA THNCPCLRDLQPDQARRPDYAVFMLKYFMCLVVGITSGVWVWSGKTLESWRALCTRCCWASKGAAVGAGA GGSGPGGGGPGPGGGGGHGGGGGSLYSDVSTGLTWRSGTASSVSYPKQMPLSQV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001044251 |
| ORF Size | 2052 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001044251.2, NP_001037716.1 |
| RefSeq Size | 3012 bp |
| RefSeq ORF | 2055 bp |
| Locus ID | 364754 |
| UniProt ID | Q498S8 |
| MW | 73 kDa |
| Gene Summary | Receptor for Wnt proteins. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalsomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Coreceptor along with RYK of Wnt proteins, such as WNT1 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN213682 | Fzd8 (untagged ORF) - Rat frizzled homolog 8 (Drosophila) (Fzd8), (10 ug) |
CNY 8,840.00 |
|
| RR213682L3 | Lenti ORF clone of Fzd8 (Myc-DDK-tagged ORF) - Rat frizzled homolog 8 (Drosophila) (Fzd8), (10 ug) |
CNY 10,550.00 |
|
| RR213682L4 | Lenti ORF clone of Fzd8 (mGFP-tagged ORF) - Rat frizzled homolog 8 (Drosophila) (Fzd8), (10 ug) |
CNY 11,690.00 |
