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Ncam1 (NM_031521) Rat Tagged ORF Clone
CAT＃: RR213674
- TrueORF®
Ncam1 (Myc-DDK-tagged ORF) - Rat neural cell adhesion molecule 1 (Ncam1), (10 ug)
"NM_031521" in other vectors (3)
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CNY 10,930.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Cd56; N-CAM; N-CAM-1; Ncam; NCAM-1; NCAM-C; NCAMC |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR213674 representing NM_031521
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCGAACTAAGGATCTCATCTGGACTTTGTTTTTCCTGGGAACTGCAGTTTCCCTGCAGGTAGATA TTGTTCCCAGCCAAGGAGAAATCAGCGTTGGAGAGTCCAAATTCTTCCTGTGTCAAGTGGCAGGAGATGC CAAAGATAAGGACATCTCCTGGTTCTCCCCCAACGGGGAGAAACTGAGCCCAAACCAGCAGCGGATCTCA GTGGTGTGGAACGATGATGACTCCTCTACCCTCACCATCTACAACGCCAACATTGATGATGCCGGCATTT ACAAGTGCGTGGTCACCGCTGAAGACGGCACCCAGTCCGAGGCCACTGTCAATGTGAAGATCTTCCAGAA GCTCATGTTCAAGAATGCGCCAACCCCACAGGAGTTTAAGGAAGGGGAAGATGCTGTGATTGTCTGTGAT GTGGTCAGCTCTCTGCCCCCAACCATCATCTGGAAACACAAAGGCCGAGATGTCATCCTGAAAAAAGATG TCCGGTTCATAGTCCTATCCAACAACTACCTGCAGATCCGAGGCATCAAGAAAACAGATGAGGGCACTTA CCGCTGTGAAGGCAGGATCCTGGCCCGGGGGGAGATCAACTTCAAGGACATTCAGGTCATTGTGAATGTA CCACCCACTGTCCAGGCCAGACAGAGCATCGTGAATGCCACTGCCAACCTGGGCCAGTCTGTCACCCTGG TGTGTGATGCCGATGGCTTCCCAGAGCCCACCATGAGCTGGACAAAGGATGGGGAACCCATAGAGAATGA GGAGGAAGATGACGAGAAGCACATCTTCAGTGACGACAGTTCGGAGCTGACCATCAGGAATGTGGACAAA AATGACGAAGCCGAATACGTCTGCATCGCTGAGAACAAGGCTGGCGAGCAGGATGCCTCCATCCACCTCA AGGTCTTCGCAAAGCCCAAAATCACCTATGTAGAGAATCAGACAGCCATGGAACTAGAGGAGCAAGTCAC TCTGACATGTGAAGCCTCCGGAGACCCCATTCCTTCCATCACCTGGAGAACGTCCACCCGAAACATCAGC AGTGAAGAAAAGGCATCGTGGACTCGACCAGAGAAGCAAGAGACTCTAGATGGGCACATGGTGGTACGCA GCCATGCTCGTGTGTCCTCCCTGACCCTGAAGAGCATCCAGTACACAGATGCTGGAGAATACATCTGCAC TGCCAGCAACACCATCGGCCAGGACTCCCAGTCCATGTACCTTGAAGTTCAATATGCTCCCAAGCTCCAG GGCCCTGTAGCTGTGTACACTTGGGAAGGGAACCAGGTGAACATCACCTGTGAGGTCTTTGCCTACCCAA GTGCCACAATCTCCTGGTTCCGAGATGGCCAGTTGCTGCCAAGCTCCAACTACAGCAATATCAAGATCTA CAACACCCCATCTGCGAGCTATCTGGAGGTAACCCCTGATTCCGAAAATGACTTTGGAAACTACAACTGC ACAGCGGTGAACCGTATTGGACAGGAGTCCTTGGAATTCATCCTGGTTCAAGCAGATACACCATCTTCCC CATCCATCGACCGGGTGGAACCATACTCCAGCACAGCACAGGTACAATTTGATGAGCCAGAAGCCACAGG TGGAGTTCCCATCCTCAAATACAAGGCTGAGTGGAAGTCGCTGGGTGAAGAAGCATGGCATTCCAAGTGG TATGATGCCAAAGAAGCCAACATGGAAGGGATTGTCACCATCATGGGCCTGAAGCCTGAGACAAGGTACG CGGTACGACTGGCGGCCCTCAACGGCAAGGGGCTGGGCGAGATCAGTGCAGCCACTGAGTTCAAGACACA GCCAGTCCGGGAACCCAGCGCACCCAAGCTGGAAGGGCAGATGGGAGAGGACGGGAACTCCATCAAGGTG AACCTGATCAAGCAGGATGACGGCGGCTCCCCCATCAGACACTATCTGGTCAAGTACAGAGCGCTCGCCT CCGAGTGGAAACCAGAGATCAGGCTCCCGTCCGGCAGTGACCACGTCATGCTCAAGTCCCTAGACTGGAA CGCCGAGTACGAAGTATATGTGGTAGCTGAGAACCAGCAAGGAAAATCCAAGGCAGCTCACTTCGTGTTC AGGACTTCAGCCCAGCCCACGGCCATCCCAGCCAATGGCAGCCCCACTGCAGGCCTGAGCACAGGCGCCA TTGTGGGCATCCTCATTGTCATTTTCGTCCTACTCCTGGTGGTCATGGACATCACCTGCTACTTCCTGAA CAAGTGTGGCCTGCTCATGTGCATCGCTGTTAACCTGTGCGGCAAAGCGGGGCCCGGAGCCAAGGGCAAA GACATGGAGGAGGGCAAGGCTGCTTTCTCGAAAGATGAGTCTAAAGAACCCATTGTAGAGGTCCGAACGG AGGAGGAACGGACTCCAAACCATGACGGAGGGAAGCACACAGAGCCCAACGAGACCACACCACTGACAGA GCCCGAGAAGGGTCCTGTAGAAACAAAGTCCGAGCCCCAGGAGTCAGAAGCAAAGCCAGCGCCAACTGAA GTCAAGACGGTCCCCAATGAAGCCACACAAACGAAAGAGAATGAGAGCAAAGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR213674 representing NM_031521
Red=Cloning site Green=Tags(s) MLRTKDLIWTLFFLGTAVSLQVDIVPSQGEISVGESKFFLCQVAGDAKDKDISWFSPNGEKLSPNQQRIS VVWNDDDSSTLTIYNANIDDAGIYKCVVTAEDGTQSEATVNVKIFQKLMFKNAPTPQEFKEGEDAVIVCD VVSSLPPTIIWKHKGRDVILKKDVRFIVLSNNYLQIRGIKKTDEGTYRCEGRILARGEINFKDIQVIVNV PPTVQARQSIVNATANLGQSVTLVCDADGFPEPTMSWTKDGEPIENEEEDDEKHIFSDDSSELTIRNVDK NDEAEYVCIAENKAGEQDASIHLKVFAKPKITYVENQTAMELEEQVTLTCEASGDPIPSITWRTSTRNIS SEEKASWTRPEKQETLDGHMVVRSHARVSSLTLKSIQYTDAGEYICTASNTIGQDSQSMYLEVQYAPKLQ GPVAVYTWEGNQVNITCEVFAYPSATISWFRDGQLLPSSNYSNIKIYNTPSASYLEVTPDSENDFGNYNC TAVNRIGQESLEFILVQADTPSSPSIDRVEPYSSTAQVQFDEPEATGGVPILKYKAEWKSLGEEAWHSKW YDAKEANMEGIVTIMGLKPETRYAVRLAALNGKGLGEISAATEFKTQPVREPSAPKLEGQMGEDGNSIKV NLIKQDDGGSPIRHYLVKYRALASEWKPEIRLPSGSDHVMLKSLDWNAEYEVYVVAENQQGKSKAAHFVF RTSAQPTAIPANGSPTAGLSTGAIVGILIVIFVLLLVVMDITCYFLNKCGLLMCIAVNLCGKAGPGAKGK DMEEGKAAFSKDESKEPIVEVRTEEERTPNHDGGKHTEPNETTPLTEPEKGPVETKSEPQESEAKPAPTE VKTVPNEATQTKENESKA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_031521 |
| ORF Size | 2574 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_031521.1, NP_113709.1 |
| RefSeq Size | 3170 bp |
| RefSeq ORF | 2577 bp |
| Locus ID | 24586 |
| UniProt ID | P13596 |
| MW | 94.7 kDa |
| Gene Summary | cell adhesion molecule; involved in neuronal cell adhesion [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN213674 | Ncam1 (untagged ORF) - Rat neural cell adhesion molecule 1 (Ncam1), (10 ug) |
CNY 10,930.00 |
|
| RR213674L3 | Lenti ORF clone of Ncam1 (Myc-DDK-tagged ORF) - Rat neural cell adhesion molecule 1 (Ncam1), (10 ug) |
CNY 12,830.00 |
|
| RR213674L4 | Lenti ORF clone of Ncam1 (mGFP-tagged ORF) - Rat neural cell adhesion molecule 1 (Ncam1), (10 ug) |
CNY 13,970.00 |
