Dip2c (NM_001107360) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1560155
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR213575 representing NM_001107360 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACCGCAGCCTGGAAGGCATGGCTCTGCCGTTGGAGGTGAGGGCCCGGCTGGCCGAGCTGGAGC TGGAGCTGTCGGAAGGTGACATTACACAAAAGGGATATGAAAAGAAGAGGTCAAAATTAATTGGAGCCTA CCTTCCCCAGCCTCCAACAGCGAATGGAGCTGCCGTGGTTCGGTGTAGACTGCAGCCCGGTGAAGGCGTT CCGAGGAGACCTTTCCGCTCTGCCCACATCGGGGTGTGCGACATCCGAGAAGCTGCAGCCCGGGAGCGGG CGGCCAGTGCAGCGGGGAACCGGCCTCTGTTTTACTTTCGTTTCGGAGTGGACCAAGCTCTGCCTCAAGA GCGACGTGCACCCGTCACTCCTTCCTCAGCTTCTCGCTACCACCGCCGAAGATCCTCAGGGTCTCGAGAT GAACGTTATCGGTCAGATGTTCATACAGAAGCTGTCCAGGCAGCTCTTGCTAAACACAAAGAAAGGAAGA TGGCAGTCCCCATGCCTTCCAAACGAAGATCCCTGGTTGTACAGACCTCCATGGATGCCTACACTCCCCC AGATACCTCTTCCGGTTCAGAAGATGAAGGCTCAGTGCAGGGTGATTCCCAGGGAACCCCCACTTCTAGC CAGGGCAGCATCAACATGGAGCATTGGATCAGTCAGGCCATCCATGGCTCTACCACATCAACAACCTCCT CCTCCTCCACTCAGAGTGGGGGCAGTGGGGCAGCACACCGGCTGGCAGATGTCATGGCCCAGACACACAT AGAAAATCATTCTGCACCTCCTGATGTAACCACATACACCTCAGAACACTCAATACAAGTGGAAAGGCCA CAGGGCTCTACCACATCCCGGACAGCACCAAAGTATGGCAATGCTGAGCTCATGGAGACTGGGGATGGTG TACCAGTAAGTAGCCGGGTATCAGCAAAAATTCAGCAGCTTGTCAATACCCTTAAACGACCGAAGAGACC ACCATTACGGGAATTCTTTGTTGATGACTTTGAGGAATTGTTAGAAGTTCAACAACCAGATCCAAACCAA CCAAAGCCAGAGGGCGCCCAGATGCTGGCCACAAGAGGGGAGCAGTTGGGAGTCGTCACAAACTGGCCAC CCTCTTTAGAAGCTGCACTACAACGATGGGGGACCATCTCTCCGAAGGCTCCCTGCCTGACTACCATGGA CACAAATGGCAAACCCCTGTACATCCTCACTTACGGCAAACTATGGACAAGAAGTATGAAGGTTGCTTAC AACATTCTACATAAATTAGGTACAAAGCAAGAACCTATGGTACGGCCTGGAGACAGGGTGGCGCTAGTGT TCCCAAACAATGACCCTGCTGCTTTCATGGTGGCCTTCTATGGTTGCTTGCTGGCTGAAGTTGTTCCTGT CCCCATTGAAGTGCCACTCACAAGAAAGGATGCAGGGAGCCAACAGATAGGTTTCTTGCTTGGAAGCTGT GGAGTAACTGTTGCCTTGACTAGTGATGCCTGTCACAAAGGACTGCCAAAAAGCCCAACAGGAGAGATCC CACAGTTCAAAGGTTGGCCAAAGCTGCTATGGTTTGTCACAGAATCAAAACACCTCTCTAAACCTCCTCG GGATTGGTTTCCACACATCAAAGATGCTAATAACGACACAGCTTATATTGAGTATAAGACGTGTAAAGAC GGAAGTGTTCTTGGTGTGACAGTGACGAGGATAGCGCTGCTGACACACTGCCAGGCACTTACACAGGCCT GCGGCTATACAGAAGCTGAAACCATTGTGAATGTTCTAGACTTCAAGAAGGATGTGGGACTCTGGCATGG CATACTGACAAGTGTCATGAACATGATGCACGTCATCAGCATCCCATACGCGCTGATGAAGGTAAACCCC CTCTCCTGGATCCAGAAGGTCTGCCAGTATAAAGCAAAAGTGGCTTGTGTGAAATCAAGAGACATGCACT GGGCACTTGTAGCACACAGAGATCAGAGAGATGTCAACCTGTCCTCCCTGCGCATGTTAATAGTGGCCGA TGGAGCAAATCCCTGGTCTATTTCTTCTTGTGATGCGTTTCTCAATGTCTTCCAGAGTAAAGGCCTTCGA CAGGAGGTCATCTGTCCCTGTGCCAGCTCACCAGAGGCCCTCACTGTGGCAATCCGGAGGCCTACAGATG ACAGCAACCAGCCACCAGGGCGAGGTGTGCTATCCATGCATGGGCTGACCTATGGTGTGATCCGTGTGGA TTCCGAGGAGAAGCTGTCCGTGCTCACCGTGCAGGATGTCGGCCTTGTCATGCCTGGAGCCATCATGTGT TCAGTGAAGCCGGATGGGGTGCCTCAGCTGTGCAGGACGGATGAAATTGGGGAGCTCTGTGTCTGTGCAG TTGCCACAGGAACGTCATATTATGGTCTCTCGGGCATGACCAAGAACACCTTTGAGGTATTTCCCATGAC AAGCTCTGGGGCTCCAATCAGTGAATACCCATTCATAAGGACAGGTTTGCTGGGATTTGTTGGTCCTGGG GGCCTCGTCTTTGTTGTGGGTAAGATGGACGGCCTGATGGTAGTGAGTGGACGAAGACACAATGCAGATG ACATTGTGGCCACAGCCCTAGCTGTAGAGCCCATGAAGTTTGTCTACAGAGGAAGGATAGCTGTGTTCTC AGTGACTGTCCTTCATGATGAAAGGATAGTGATTGTGGCTGAGCAGAGGCCGGATTCCACAGAAGAAGAC AGTTTCCAGTGGATGAGCAGAGTACTCCAGGCAATTGACAGTATACATCAAGTTGGAGTTTATTGCCTGG CCTTAGTCCCAGCAAATACCCTTCCCAAAACCCCACTTGGTGGCATCCATTTGTCAGAAACAAAGCAGCT TTTCCTGGAGGGCTCCCTTCACCCCTGCAATGTCCTGATGTGCCCACACACCTGTGTCACAAATTTACCG AAACCTCGGCAGAAGCAGCCAGAAATTGGCCCTGCCTCAGTGATGGTGGGAAACCTAGTGTCTGGGAAGA GGATTGCCCAGGCTAGTGGCAGAGACCTGGGTCAGATAGAAGACAATGACCAGGCCCGGAAGTTCCTATT CCTCTCAGAGGTCTTACAGTGGAGAGCGCAGACCACTCCAGACCACCTGCTGTACACTCTGCTCAATTGT CGGGGAACAATCGCAAATTCCTTGACGTGTGTTCAACTCCACAAGCGTGCAGAAAAGATAGCAGTGATGC TGATGGAGAGGGGCCACCTGCAGGACGGAGACCACGTGGCATTAGTCTACCCTCCAGGAATAGACCTTAT TGCTGCATTTTATGGTTGCCTGTATGCGGGCTGTGTGCCAATCACCGTGCGACCTCCACATCCACAGAAC ATTGCAACAACATTGCCTACAGTCAAGATGATCGTAGAGGTGAGTCGGTCTGCTTGTCTCATGACAACAC AATTGATTTGTAAACTGCTTCGGTCCAGAGAGGCAGCAGCAGCTGTAGATGTCAGGACATGGCCCCTCAT ACTGGACACAGATGATTTGCCAAAGAAGCGGCCTGCCCAGATCTACAAACCTTCCAACCCAGACACACTG GCTTACCTTGACTTTAGTGTGTCCACAACTGGGATGCTAGCTGGTGTAAAGATGTCTCATGCAGCCACCA GTGCCTTTTGCCGTTCTATTAAGCTGCAGTGTGAACTTTACCCTTCTAGAGAAGTGGCCATCTGTCTGGA CCCTTACTGTGGACTTGGGTTTGTCCTCTGGTGTCTCTGCAGTGTATACTCAGGGCACCAGTCCATTCTG ATCCCACCTTCTGAGCTGGAGACCAATCCTGCCTTGTGGCTTCTTGCTGTGAGTCAGTACAAAGTCCGGG ATACCTTCTGTTCCTATTCAGTGATGGAGCTTTGCACCAAAGGGCTGGGCTCACAAACTGAGTCCCTTAA GGCACGAGGACTGGACTTGTCCCGAGTAAGAACCTGTGTGGTTGTGGCAGAAGAGCGTCCCCGAATCGCT CTTACACAGTCCTTCTCAAAGCTCTTTAAGGACTTGGGCCTCCACCCACGAGCTGTTAGCACTTCATTTG GCTGCAGAGTGAACCTGGCAATTTGCTTACAGGGGACATCAGGACCTGACCCCACCACTGTCTATGTTGA CATGAGAGCCCTGAGACATGACAGAGTCCGGTTGGTAGAAAGGGGATCCCCTCACAGCTTACCTCTGATG GAGTCTGGAAAAATACTTCCTGGAGTCCGGATCATTATTGCCAATCCAGAAACGAAAGGCCCCCTGGGGG ACTCCCACCTTGGAGAGATCTGGGTCCACAGCGCCCACAATGCCAGTGGCTATTTTACCATTTATGGAGA TGAATCTCTGCAGTCCGATCATTTCAACTCCCGGTTGAGTTTTGGAGACACCCAGACCATCTGGGCCCGA ACAGGCTACCTGGGCTTCCTGCGGAGAACTGAGCTCACAGATGCAAATGGAGAGCGCCATGATGCCCTCT ATGTGGTAGGAGCACTGGATGAAGCCATGGAGCTGCGGGGCATGAGGTACCACCCAATAGACATAGAGAC TTCAGTCATCAGAGCCCACAAAAGTGTCACAGAATGTGCTGTCTTTACCTGGACAAACCTGCTAGTGGTT GTGGTTGAACTGGATGGGTCAGAGCAGGAAGCCTTGGACTTGGTTCCCTTGGTGACCAATGTGGTCCTGG AAGAGCATTACCTGATTGTAGGCGTAGTAGTCGTGGTAGACATCGGTGTCATCCCCATCAACTCCCGGGG AGAAAAACAGCGGATGCACCTACGGGACGGGTTCTTGGCCGACCAGCTGGATCCCATCTACGTGGCCTAT AACATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR213575 representing NM_001107360 Red=Cloning site Green=Tags(s) MADRSLEGMALPLEVRARLAELELELSEGDITQKGYEKKRSKLIGAYLPQPPTANGAAVVRCRLQPGEGV PRRPFRSAHIGVCDIREAAARERAASAAGNRPLFYFRFGVDQALPQERRAPVTPSSASRYHRRRSSGSRD ERYRSDVHTEAVQAALAKHKERKMAVPMPSKRRSLVVQTSMDAYTPPDTSSGSEDEGSVQGDSQGTPTSS QGSINMEHWISQAIHGSTTSTTSSSSTQSGGSGAAHRLADVMAQTHIENHSAPPDVTTYTSEHSIQVERP QGSTTSRTAPKYGNAELMETGDGVPVSSRVSAKIQQLVNTLKRPKRPPLREFFVDDFEELLEVQQPDPNQ PKPEGAQMLATRGEQLGVVTNWPPSLEAALQRWGTISPKAPCLTTMDTNGKPLYILTYGKLWTRSMKVAY NILHKLGTKQEPMVRPGDRVALVFPNNDPAAFMVAFYGCLLAEVVPVPIEVPLTRKDAGSQQIGFLLGSC GVTVALTSDACHKGLPKSPTGEIPQFKGWPKLLWFVTESKHLSKPPRDWFPHIKDANNDTAYIEYKTCKD GSVLGVTVTRIALLTHCQALTQACGYTEAETIVNVLDFKKDVGLWHGILTSVMNMMHVISIPYALMKVNP LSWIQKVCQYKAKVACVKSRDMHWALVAHRDQRDVNLSSLRMLIVADGANPWSISSCDAFLNVFQSKGLR QEVICPCASSPEALTVAIRRPTDDSNQPPGRGVLSMHGLTYGVIRVDSEEKLSVLTVQDVGLVMPGAIMC SVKPDGVPQLCRTDEIGELCVCAVATGTSYYGLSGMTKNTFEVFPMTSSGAPISEYPFIRTGLLGFVGPG GLVFVVGKMDGLMVVSGRRHNADDIVATALAVEPMKFVYRGRIAVFSVTVLHDERIVIVAEQRPDSTEED SFQWMSRVLQAIDSIHQVGVYCLALVPANTLPKTPLGGIHLSETKQLFLEGSLHPCNVLMCPHTCVTNLP KPRQKQPEIGPASVMVGNLVSGKRIAQASGRDLGQIEDNDQARKFLFLSEVLQWRAQTTPDHLLYTLLNC RGTIANSLTCVQLHKRAEKIAVMLMERGHLQDGDHVALVYPPGIDLIAAFYGCLYAGCVPITVRPPHPQN IATTLPTVKMIVEVSRSACLMTTQLICKLLRSREAAAAVDVRTWPLILDTDDLPKKRPAQIYKPSNPDTL AYLDFSVSTTGMLAGVKMSHAATSAFCRSIKLQCELYPSREVAICLDPYCGLGFVLWCLCSVYSGHQSIL IPPSELETNPALWLLAVSQYKVRDTFCSYSVMELCTKGLGSQTESLKARGLDLSRVRTCVVVAEERPRIA LTQSFSKLFKDLGLHPRAVSTSFGCRVNLAICLQGTSGPDPTTVYVDMRALRHDRVRLVERGSPHSLPLM ESGKILPGVRIIIANPETKGPLGDSHLGEIWVHSAHNASGYFTIYGDESLQSDHFNSRLSFGDTQTIWAR TGYLGFLRRTELTDANGERHDALYVVGALDEAMELRGMRYHPIDIETSVIRAHKSVTECAVFTWTNLLVV VVELDGSEQEALDLVPLVTNVVLEEHYLIVGVVVVVDIGVIPINSRGEKQRMHLRDGFLADQLDPIYVAY NM TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001107360
ORF Size	4836 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107360.1, NP_001100830.1
RefSeq Size	6107 bp
RefSeq ORF	4839 bp
Locus ID	307067
MW	176.9 kDa

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