Oplah (NM_053904) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	MGC105359
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR213448 representing NM_053904 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAGCCCAGAAGGGCGCTTCCATTTCGCCATCGACCGCGGTGGCACCTTCACAGATGTCTTTGCCC AGTGCCCTGGAGGGCATGTGCGTGTCCTGAAGCTGCTCTCAGAGGACCCTGCCAACTATCCAGATGCACC CACAGAGGGCATCCGCCGAATTCTAGAGCAGGAGGAGGGTGTGCTGCTGCCTCGAGGCCGACCGCTAGAC ACCAGTCGCATTGCCAGCATCCGCATGGGTACCACGGTGGCCACCAATGCACTGTTGGAACGACAGGGAG AACGGGTGGCACTGCTGGTGACTCGGGGTTTCCGAGACCTGCTGCATATTGGCACTCAGGCCCGCCCGGA CCTCTTTGACTTGGCTGTGCCCATGCCAGAGGTTCTGTATGAGGAAGTGCTGGAGGTAGATGAGCGAGTG GTGCTGTATCGCGGAGAACCAGGTGCCGGCTCTCCTGTCAAAGGCCGCACAGGGGACCTGCTAGAGATAC AGCAGCCTGTGGACCTGGAAGCCCTGCGTGGGAAGCTGGAGGGGCTCTTGTCTCGGGGCATTCACAGTCT GGCAGTGGTGCTCATGCATTCGTACACGTGGGCCCAGCATGAGCAGCAGGTGGGCACGCTGGCCCGGGAG CTGGGCTTCACGCACGTCTCCTTGTCCTCGGAAGTCATGCCCATGGTACGAATTGTTCCTCGGGGCCATA CAGCCTGTGCTGACGCTTACCTTACTCCCACCATCCAGCGCTATGTGCAGGGCTTCCGCCGAGGCTTCCA GGGCCAGCTAAAGAATGTGCAAGTTCTCTTCATGCGCTCTGATGGTGGCCTCGCACCCATGGATGCTTTC AGTGGTTCCCGGGCTGTGCTCTCTGGCCCTGCTGGGGGTGTGGTTGGCTACTCAGCTACCACCTACCATC TGGAAGGCGGTCAGCCTGTCATTGGCTTTGACATGGGAGGCACATCCACAGACGTGAGCCGCTATGCTGG AGAATTTGAGCATGTCTTTGAGGCTAGCACAGCAGGCGTTACCCTTCAGGCACCCCAGTTGGACATCAAC ACAGTGGCAGCTGGCGGGGGTTCCCGCCTCTTCTTCAGATCTGGCCTCTTTGTGGTTGGTCCAGAGTCAG CAGGTGCCCACCCAGGTCCTGCCTGCTACCGTAAAGGGGGTCCTGTGACAGTGACAGATGCTAATCTGGT CCTGGGTCGCCTGCTGCCTGCCTCCTTCCCCTGCATTTTTGGGCCAGGAGAAGACCAGCCACTGTCTCCT GAGGCTTCCCGAAAGGCTCTAGAGGCTGTGGCCATGGAGGTCAACAGTTTCTTGACCAATGGACCGTGCC CAGCTTCCCAACTAAGTCTGGAAGAGGTGGCCATGGGGTTTGTGCGTGTTGCCAATGAAGCCATGTGCCG GCCTATCCGTGCCCTCACACAGGCACGAGGCCATGACCCCTCAGCCCATGTATTGGCTTGCTTTGGAGGA GCTGGTGGGCAACACGCTTGTGCCATTGCCCGGGCCCTGGGGATGGATACTGTGCACATTCACAGGCACA GCGGGCTGCTGTCAGCACTAGGACTGGCCTTGGCAGATGTGGTTCACGAAGCACAGGAGCCCTGTTCCCT GTCTTACACACCTGAAACCTTTGCACAACTGGACCAGAGACTGAGCCGCCTGGAGGAGCAGTGTGTGGAT GCCTTGCAGGTCCAGGGCTTCCCTAGGTCTCAGATCAGCACCGAGAGCTTCCTGCATCTTCGCTACCAAG GCACTGACTGCGCCCTAATGGTGTCTGCCCATCAGCATCCGGCCACAGCCTGCTCACCCCGAGCTGGTGA CTTTGGAGCCGCATTTGTGGAGAGGTACATGAGAGAGTTTGGCTTCATTATCCCCGAGCGGCCGGTGGTG GTAGATGATGTACGTGTGAGGGGAACTGGCCGTAGTGGACTTCAGCTGGAGGACACCCCCAAAATCCAGA CTGGACCTCCCCACGTGGAAAAGGTGACCCAGTGCTACTTTGAAGGGGGTTATCAGGAGACTCCCGTGTA CCTTTTAGGAGAACTAGGCTACGGGCACCAGCTCCAAGGGCCCTGCCTTATCATCGACAACAACAGCACC ATCCTTGTAGAACCGGGTTGCCAAGCAGAGGTGACTGATACAGGGGACATCCGCATTTCTGTGGGAGCTG AGGGTCCTAGTATGGCAGATACCAGGCTTGACCCCATCCAGCTGTCTATTTTCTCACACCGCTTCATGAG CATTGCTGAGCAGATGGGCCGCATCCTACAGCGCACAGCCATCTCTACCAACATCAAGGAACGCCTCGAC TTCTCCTGTGCCCTCTTTGGGCCAGATGGGGGCCTCGTCTCCAATGCTCCCCACATTCCTGTGCACCTGG GTGCCATGCAAGAGACTGTACAGTTCCAGATTCAGCACTTAGGAGCCGACCTCCATCCTGGTGATGTGTT GCTCAGCAACCATCCCAGCGCAGGGGGCAGCCATCTTCCTGACCTGACTGTCATTACACCGGTGTTTTGG CCAGGCCAGACGAGGCCTGTGTTCTACGTGGCTAGCCGAGGGCACCACGCAGACATTGGAGGAATCACAC CGGGCTCTATGCCGCCTCACTCCACCACGCTGCAACAGGAGGGTGCCGTTTTTCTGTCCTTCAAACTGGT CCAGGGAGGCGTCTTCCAGGAAGAGGCAGTGACAGAGGCCCTACGGGCACCAGGCAAGATCTCTGGCTGT AGTGGAACCAGGAACCTGCATGACAACCTGTCGGATCTTCGTGCCCAGGTGGCAGCTAACCAGAAAGGCA TCCAGCTGGTGGGAGAGCTGATCGGACAGTATGGCTTAGATGTGGTGCAGGCCTATATGGGCCATATTCA GGCGAATGCTGAGCTAGCAGTGAGAGACATGCTCCGGGCTTTTGGAACTTCCCGGCAGGCCAGGGGCCTG CCCCTGGAGGTGTCTGCAGAGGATCACATGGATGATGGCTCTCCCATCTGTCTGCGTGTTCAGATCAACC TGAGTCAGGGCAGTGCGGTATTTGACTTTACTGGTTCCGGGTCTGAGGTGTTTGGCAATCTCAATGCCCC GAGAGCCATAACACTGTCTGCTCTCATCTATTGCTTACGCTGTCTAGTGGGCCGTGACATCCCACTTAAC CAGGGTTGCCTGGCTCCTGTGCGTGTCATAATTCCCAAAGGCTCCATATTGGATCCATCCCCAGAGGCAG CAGTGGTCGGCGGCAACGTGCTCACATCTCAGCGAGTAGTGGATGTCATTCTGGGGGCTTTTGGGGCCTG TTCAGCCTCCCAGGGCTGCATGAACAATGTGACCCTGGGCAATGCCCGTATGGGCTACTATGAGACAGTG GCTGGTGGTGCCGGTGCGGGCCCTGGCTGGCATGGGCGCAGTGGTGTACACAGTCACATGACCAACACAC GCATTACGGATCCAGAGATTCTGGAGAGTCGGTATCCAGTTATCCTGCGCCGCTTTGAGCTGAGGCCAGG CTCCGGGGGCCGAGGTCGCTTCCGGGGAGGTGATGGCGTAGTCCGAGAGCTGGTCTTTCGGGAAGAGGCG CTGTTGTCTGTGCTCACCGAGCGCCGGGCCTTCCAGCCTTACGGCCTCCACGGGGGAGAGCCTGGTGCGC GTGGCTTAAACCTCCTGATCAGAAAAGATGGGCGCACAGTGAATTTGGGCGGCAAGACATCTGTGACCGT GTACCCCGGGGACGTGTTCTGCCTCCACACGCCTGGGGGTGGGGGCTACGGAGACCCGGAGGATCCAGCG CCACCACCAGGCTCGCCCCCGCTATTTCCAGCCTTCCCCGAGCGCGGCAGTGTATTCGAGTACCGCCGCG CCCAGGAAGCCGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR213448 representing NM_053904 Red=Cloning site Green=Tags(s) MGSPEGRFHFAIDRGGTFTDVFAQCPGGHVRVLKLLSEDPANYPDAPTEGIRRILEQEEGVLLPRGRPLD TSRIASIRMGTTVATNALLERQGERVALLVTRGFRDLLHIGTQARPDLFDLAVPMPEVLYEEVLEVDERV VLYRGEPGAGSPVKGRTGDLLEIQQPVDLEALRGKLEGLLSRGIHSLAVVLMHSYTWAQHEQQVGTLARE LGFTHVSLSSEVMPMVRIVPRGHTACADAYLTPTIQRYVQGFRRGFQGQLKNVQVLFMRSDGGLAPMDAF SGSRAVLSGPAGGVVGYSATTYHLEGGQPVIGFDMGGTSTDVSRYAGEFEHVFEASTAGVTLQAPQLDIN TVAAGGGSRLFFRSGLFVVGPESAGAHPGPACYRKGGPVTVTDANLVLGRLLPASFPCIFGPGEDQPLSP EASRKALEAVAMEVNSFLTNGPCPASQLSLEEVAMGFVRVANEAMCRPIRALTQARGHDPSAHVLACFGG AGGQHACAIARALGMDTVHIHRHSGLLSALGLALADVVHEAQEPCSLSYTPETFAQLDQRLSRLEEQCVD ALQVQGFPRSQISTESFLHLRYQGTDCALMVSAHQHPATACSPRAGDFGAAFVERYMREFGFIIPERPVV VDDVRVRGTGRSGLQLEDTPKIQTGPPHVEKVTQCYFEGGYQETPVYLLGELGYGHQLQGPCLIIDNNST ILVEPGCQAEVTDTGDIRISVGAEGPSMADTRLDPIQLSIFSHRFMSIAEQMGRILQRTAISTNIKERLD FSCALFGPDGGLVSNAPHIPVHLGAMQETVQFQIQHLGADLHPGDVLLSNHPSAGGSHLPDLTVITPVFW PGQTRPVFYVASRGHHADIGGITPGSMPPHSTTLQQEGAVFLSFKLVQGGVFQEEAVTEALRAPGKISGC SGTRNLHDNLSDLRAQVAANQKGIQLVGELIGQYGLDVVQAYMGHIQANAELAVRDMLRAFGTSRQARGL PLEVSAEDHMDDGSPICLRVQINLSQGSAVFDFTGSGSEVFGNLNAPRAITLSALIYCLRCLVGRDIPLN QGCLAPVRVIIPKGSILDPSPEAAVVGGNVLTSQRVVDVILGAFGACSASQGCMNNVTLGNARMGYYETV AGGAGAGPGWHGRSGVHSHMTNTRITDPEILESRYPVILRRFELRPGSGGRGRFRGGDGVVRELVFREEA LLSVLTERRAFQPYGLHGGEPGARGLNLLIRKDGRTVNLGGKTSVTVYPGDVFCLHTPGGGGYGDPEDPA PPPGSPPLFPAFPERGSVFEYRRAQEAV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_053904
ORF Size	3864 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053904.1, NP_446356.1
RefSeq Size	4003 bp
RefSeq ORF	3867 bp
Locus ID	116684
UniProt ID	P97608
MW	137.7 kDa
Gene Summary	catalyzes cleavage of 5-oxo-L-proline to form L-glutamate and is coupled to the hydrolysis of ATP to ADP and inorganic phosphate [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.