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Tpcn1 (NM_139332) Rat Tagged ORF Clone
CAT＃: RR213405
- TrueORF®
Tpcn1 (Myc-DDK-tagged ORF) - Rat two pore segment channel 1 (Tpcn1), (10 ug)
"NM_139332" in other vectors (3)
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CNY 10,450.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR213405 representing NM_139332
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGTAATTTTAGACGACGACGTGCTGCTCATCCTGACCTTGGACGAGGAGCTGAGTGCGCCGCTGA CTCCTTCCAACGGCCTGGGCCAAGAAGACCTGCCCAGCAAAAATGGGGGCGGCCAAAGCGGCCCCAACTC CCAAGTTCCCAGTCTGGTCTCTGGAGCCGACAGTCCCCCCTCCAGTCCCCCCGGACACAACTGGGAGATG AATTATCAAGAGGCAGCAATCTACCTCCAGGAAGGCCAGAACAATGACAAGTTCTTCACCCACCCCAAGG ATGCCAGAGCGCTGGCTGCCTACCTCTTCGTACACAACCACTTCTTTTATATGATGGAGCTGCTCACAGC CCTGCTTCTGCTGCTGCTGTCACTGTGCGAGTCACCCGCTGTCCCTGCGCTCAAGCTCCGAACTTACGTC CACGCCACCCTGGAGCTGTTCGCCCTCATGGTGGTGGTATTTGAACTCTGCATGAAATTGCGGTGGCTGG GCTTCCACACATTCGTCAGGCACAAACGCACCATGGTCAAGACGTCTGTTTTGGTGGTGCAGTTCATCGA GGCCATTGTGGTGCTGGTGCGGCAGACGTCCCACGTGCGGGTGACCCGGGCACTACGCTGCATTTTCCTG GTGGACTGTCGGTACTGTGGTGGCGTGCGGCGCAACCTGCGGCAGATCTTCCAGTCGCTGCCGCCTTTCA TGGACATCCTCCTGCTGCTGCTCTTCTTTATGATCATCTTCGCCATCCTCGGTTTCTACTTATTCTCTAC GAACCCTTCCGACCCTTACTTCAACACCCTGGAGAACAGCATCGTCAACCTGTTTGTTCTCCTGACCACA GCCAACTTTCCAGACGTCATGATGCCCTCCTACTCCCGGAACCCCTGGTCCTGTGTCTTCTTTATCGTGT ACCTCTCCATTGAGCTGTACTTCATCATGAACCTGCTCCTAGCCGTGGTGTTCGACACCTTCAACGACAT CGAAAAGCACAAGTTCAAGTCCTTGCTGCTGCATAAGCGAACAGCCATCCAGCACGCATACCACCTGCTC GTCAGCCAACGGAGGCCGGCCGGCATCTCCTACAGGCAGTTTGAAGGCTTGATGCGCTTCTACAAGCCCC GGATGAGCGCTAGGGAGCGCTTCCTGACTTTCAAGGCCCTGAATCAGAGCAACACACCTCTGCTTAGCCT GAAGGACTTCTATGATATTTATGAAGTTGCTGCTCTGCAGTGGAAGGCAAAGAAGAACAGACAGCACTGG TTTGACGAGCTTCCCCGGACAGCCTTCCTCATCTTCAAAGGGATTAACATCCTTGTGAATTCCAAGGCCT TCCAGTATTTCATGTACCTGGTGGTGGCTGTCAACGGTGTCTGGATCCTAGTGGAGACATTCATGTTGAA AGGTGGGAATTTCATCTCAAAGCACGTGCCCTGGAGTTACCTCGTGTTTCTTACCATCTATGGAGTTGAG CTGTTCATGAAGGTGGCTGGCCTGGGCCCTGTGGAGTACTTGTCCTCTGGATGGAACCTGTTCGATTTCT CAGTCACAGCATTTGCCTTCCTGGGACTGCTCGCGCTGACGCTCAACATGGAGCCCTTCTACTTTATTGT GGTCCTGCGTCCCCTTCAGCTGCTGAGGTTATTCAAGTTGAAGAAACGCTACCGCAATGTGTTGGACACC ATGTTTGAGCTGCTGCCACGAATGGCCAGCCTCGGCCTCACACTGCTCACCTTCTACTACTCCTTCGCCA TCGTGGGCATGGAGTTCTTCAGTGGGCGACTGTCCCCCAACTGCTGCAACTCCAGCACGGTGGCTGACGC CTATCGATTCATCAATCACACGGTGGGCAATAAGACCAAGGTGGAGGAAGGCTACTACTATCTCAATAAC TTTGACAACATCCTCAACAGCTTTGTGACCTTGTTTGAGCTCACCGTTGTCAACAACTGGTACATCATCA TGGAAGGCGTCACCTCGCAGACTTCCCACTGGAGCCGCCTGTACTTCATGACCTTTTACATCGTGACCAT GGTGGTGATGACCATTATCGTGGCCTTCATCCTGGAGGCCTTCGTCTTCCGCATGAACTACAGCCGCAAG AGCCAGGAATCGGAAGTGGACAGTGGCATCGTCATCGAGAAGGAGATGTCCAAGGAGGAGCTTCTGGCCA TCCTGGAGCTTCATCGAGAGGCGCGAGGCACCTCCTCTGACATCACCCGGCTGCTCGACACCCTCTCTCA GATGGAGAAATACCAGCAAAACTCCATGGTGTTTCTGGGACGGCGATCGAGAACCAAAAGTGACCTGAGC CTGAAGATGTACCAGGAGGAGATCCAGGAGTGGTACGAGGAGCATGCCCGGGAACAGGAGCAGCAGCAGC TCAGGGGCAGCGCGCCAAGCCCTGCAGCCCAGCAGACCCCCGGCAGTCGCCAGCGCTCCCAGACCGTCAC C ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR213405 representing NM_139332
Red=Cloning site Green=Tags(s) MSVILDDDVLLILTLDEELSAPLTPSNGLGQEDLPSKNGGGQSGPNSQVPSLVSGADSPPSSPPGHNWEM NYQEAAIYLQEGQNNDKFFTHPKDARALAAYLFVHNHFFYMMELLTALLLLLLSLCESPAVPALKLRTYV HATLELFALMVVVFELCMKLRWLGFHTFVRHKRTMVKTSVLVVQFIEAIVVLVRQTSHVRVTRALRCIFL VDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSTNPSDPYFNTLENSIVNLFVLLTT ANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKHKFKSLLLHKRTAIQHAYHLL VSQRRPAGISYRQFEGLMRFYKPRMSARERFLTFKALNQSNTPLLSLKDFYDIYEVAALQWKAKKNRQHW FDELPRTAFLIFKGINILVNSKAFQYFMYLVVAVNGVWILVETFMLKGGNFISKHVPWSYLVFLTIYGVE LFMKVAGLGPVEYLSSGWNLFDFSVTAFAFLGLLALTLNMEPFYFIVVLRPLQLLRLFKLKKRYRNVLDT MFELLPRMASLGLTLLTFYYSFAIVGMEFFSGRLSPNCCNSSTVADAYRFINHTVGNKTKVEEGYYYLNN FDNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRK SQESEVDSGIVIEKEMSKEELLAILELHREARGTSSDITRLLDTLSQMEKYQQNSMVFLGRRSRTKSDLS LKMYQEEIQEWYEEHAREQEQQQLRGSAPSPAAQQTPGSRQRSQTVT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_139332 |
| ORF Size | 2451 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_139332.3, NP_647548.2 |
| RefSeq Size | 4735 bp |
| RefSeq ORF | 2454 bp |
| Locus ID | 246215 |
| UniProt ID | Q9WTN5 |
| MW | 94.4 kDa |
| Gene Summary | two-pore voltage-gated sodium and calcium channel [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN213405 | Tpcn1 (untagged ORF) - Rat two pore segment channel 1 (Tpcn1), (10 ug) |
CNY 10,450.00 |
|
| RR213405L3 | Lenti ORF clone of Tpcn1 (Myc-DDK-tagged ORF) - Rat two pore segment channel 1 (Tpcn1), (10 ug) |
CNY 12,260.00 |
|
| RR213405L4 | Lenti ORF clone of Tpcn1 (mGFP-tagged ORF) - Rat two pore segment channel 1 (Tpcn1), (10 ug) |
CNY 13,490.00 |
