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Hcn2 (NM_053684) Rat Tagged ORF Clone
CAT＃: RR213404
- TrueORF®
Hcn2 (Myc-DDK-tagged ORF) - Rat hyperpolarization activated cyclic nucleotide-gated potassium channel 2 (Hcn2), (10 ug)
"NM_053684" in other vectors (3)
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CNY 11,120.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR213404 representing NM_053684
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGCGCGCGGGGGCGGCGGGCGGCCGGGCGATAGTCCGGGCGCGACCCCTGCGCCGGGGCCGCCGC CACCGCCGCCGCCGCCCGCGCCCCCTCAGCCTCAGCCACCACCCGCGCCACCCCCGAACCCCACGACCCC CTCGCACCCGGAGTCGGCGGACGAGCCCGGCCCGCGCTCCCGGCTCTGCAGCCGCGACAGCTCCTGCACT CCTGGCGCGGCCAAGGGCGGCGCGAATGGCGAGTGCGGGCGCGGGGAGCCGCAGTGCAGCCCCGAGGGCC CCGCGCGCGGCCCCAAGGTTTCGTTCTCATGTCGCGGGGCGGCCTCGGGGCCCGCGGCGGCCGAGGAGGC GGGCAGCGAGGAGGCGGGCCCGGCGGGTGAGCCGCGCGGCAGCCAGGCCAGCTTCCTGCAGCGCCAATTC GGGGCGCTCCTGCAGCCGGGCGTCAACAAGTTCTCCCTGCGGATGTTTGGCAGCCAGAAGGCCGTGGAGC GCGAGCAGGAACGCGTGAAGTCGGCGGGGGCCTGGATCATCCACCCCTACAGCGACTTCAGGTTCTACTG GGACTTCACCATGCTGTTGTTCATGGTGGGAAATCTCATCATCATCCCTGTGGGCATCACTTTCTTCAAG GACGAGACCACGGCGCCCTGGATCGTCTTCAACGTGGTCTCGGACACTTTCTTCCTCATGGACTTGGTGC TGAACTTTCGCACCGGCATTGTTATTGAGGACAACACGGAGATCATCCTGGACCCCGAAAAGATAAAGAA AAAGTACCTGCGTACGTGGTTCGTGGTAGACTTCGTGTCATCCATCCCGGTGGACTACATCTTCCTCATC GTGGAGAAGGGAATCGACTCCGAGGTCTACAAGACGGCCCGTGCACTACGCATCGTGCGTTTCACCAAGA TCCTCAGTCTGCTGCGGTTGCTGCGGCTATCCCGGCTCATCCGATATATCCACCAATGGGAGGAGATTTT CCACATGACCTACGACCTGGCAAGCGCGGTGATGCGCATCTGTAACCTGATCAGCATGATGCTGCTGCTC TGCCACTGGGACGGCTGCCTGCAGTTCCTGGTGCCCATGCTGCAAGACTTCCCCAGCGACTGCTGGGTGT CCATCAACAACATGGTGAACCACTCGTGGAGCGAACTCTATTCGTTCGCGCTCTTCAAGGCCATGAGCCA CATGCTCTGTATTGGCTACGGGCGGCAGGCTCCCGAGAGCATGACGGACATCTGGCTCACCATGCTCAGC ATGATCGTGGGCGCCACCTGCTACGCTATGTTCATTGGGCACGCCACGGCGCTTATCCAGTCCCTGGACT CGTCACGGCGCCAGTACCAGGAGAAGTACAAGCAAGTGGAGCAGTACATGTCCTTCCACAAACTGCCGGC TGACTTCCGCCAGAAGATCCACGATTACTATGAACACCGGTACCAGGGGAAGATGTTTGACGAGGACAGC ATCCTGGGGGAACTCAACGGCCCACTGCGTGAGGAGATTGTGAACTTCAACTGCCGGAAGCTGGTGGCTT CCATGCCGTTGTTTGCCAACGCAGACCCCAACTTCGTCACCGCCATGCTGACAAAGCTCAAATTTGAGGT CTTCCAGCCTGGAGACTACATCATCCGAGAGGGGACCATCGGGAAGAAGATGTACTTCATCCAGCACGGG GTGGTGAGCGTGCTCACCAAGGGCAACAAGGAGATGAAGCTGTCAGATGGCTCCTATTTTGGGGAGATCT GCCTGCTCACGAGGGGCCGGCGCACAGCCAGTGTGCGGGCTGACACCTACTGTCGCCTCTACTCACTGAG CGTGGACAACTTCAACGAGGTGCTGGAGGAGTACCCCATGATGCGGCGTGCCTTTGAGACCGTGGCCATT GACCGCCTGGACCGCATAGGCAAGAAGAACTCCATCTTGCTACACAAGGTTCAGCATGATCTCAGCTCGG GTGTGTTCAACAACCAGGAGAACGCCATCATCCAGGAGATTGTCAAATATGACCGTGAGATGGTGCAGCA GGCAGAGCTGGGCCAGCGTGTGGGGCTCTTCCCACCACCGCCACCACCGCAGGTCACGTCGGCCATCGCC ACACTGCAGCAGGCCGTGGCCATGAGCTTCTGCCCGCAGGTGGCCCGCCCGCTCGTGGGGCCCCTGGCGC TAGGGTCCCCACGCCTCGTGCGCCGCGCGCCCCCAGGGCCTCTGCCTCCTGCAGCCTCACCAGGGCCACC CGCAGCGAGCCCCCCGGCTGCACCCTCGAGCCCTCGGGCACCGCGGACCTCACCCTACGGTGTGCCTGGC TCTCCGGCAACGCGTGTGGGGCCCGCATTGCCCGCACGCCGCCTGAGCCGCGCCTCGCGCCCACTGTCCG CCTCGCAGCCCTCGCTGCCCCACGGCGCGCCCGCACCCAGCCCCGCGGCCTCTGCGCGCCCGGCCAGCAG CTCCACCCCGCGTCTGGGACCCGCACCCACCACCCGGACCGCGGCACCCAGTCCGGACCGCAGGGACTCA GCCTCGCCGGGCGCTGCCAGTGGCCTCGACCCACTGGACTCTGCGCGCTCGCGCCTCTCTTCCAACTTG ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA TTACAAGGATGACGACGATAAGGTTTAA >RR213404 representing NM_053684
Red=Cloning site Green=Tags(s) MDARGGGGRPGDSPGATPAPGPPPPPPPPAPPQPQPPPAPPPNPTTPSHPESADEPGPRSRLCSRDSSCT PGAAKGGANGECGRGEPQCSPEGPARGPKVSFSCRGAASGPAAAEEAGSEEAGPAGEPRGSQASFLQRQF GALLQPGVNKFSLRMFGSQKAVEREQERVKSAGAWIIHPYSDFRFYWDFTMLLFMVGNLIIIPVGITFFK DETTAPWIVFNVVSDTFFLMDLVLNFRTGIVIEDNTEIILDPEKIKKKYLRTWFVVDFVSSIPVDYIFLI VEKGIDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVMRICNLISMMLLL CHWDGCLQFLVPMLQDFPSDCWVSINNMVNHSWSELYSFALFKAMSHMLCIGYGRQAPESMTDIWLTMLS MIVGATCYAMFIGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPADFRQKIHDYYEHRYQGKMFDEDS ILGELNGPLREEIVNFNCRKLVASMPLFANADPNFVTAMLTKLKFEVFQPGDYIIREGTIGKKMYFIQHG VVSVLTKGNKEMKLSDGSYFGEICLLTRGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMRRAFETVAI DRLDRIGKKNSILLHKVQHDLSSGVFNNQENAIIQEIVKYDREMVQQAELGQRVGLFPPPPPPQVTSAIA TLQQAVAMSFCPQVARPLVGPLALGSPRLVRRAPPGPLPPAASPGPPAASPPAAPSSPRAPRTSPYGVPG SPATRVGPALPARRLSRASRPLSASQPSLPHGAPAPSPAASARPASSSTPRLGPAPTTRTAAPSPDRRDS ASPGAASGLDPLDSARSRLSSNL TRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-NotI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_053684 |
| ORF Size | 2589 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_053684.1, NP_446136.1 |
| RefSeq Size | 2633 bp |
| RefSeq ORF | 2592 bp |
| Locus ID | 114244 |
| UniProt ID | Q9JKA9 |
| MW | 94.7 kDa |
| Gene Summary | may play a role in generation of neuronal pacemaker activity [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN213404 | Hcn2 (untagged ORF) - Rat hyperpolarization activated cyclic nucleotide-gated potassium channel 2 (Hcn2), (10 ug) |
CNY 11,120.00 |
|
| RR213404L3 | Lenti ORF clone of Hcn2 (Myc-DDK-tagged ORF) - Rat hyperpolarization activated cyclic nucleotide-gated potassium channel 2 (Hcn2), (10 ug) |
CNY 12,830.00 |
|
| RR213404L4 | Lenti ORF clone of Hcn2 (mGFP-tagged ORF) - Rat hyperpolarization activated cyclic nucleotide-gated potassium channel 2 (Hcn2), (10 ug) |
CNY 14,060.00 |
