Nfrkb (NM_001108133) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR213386 representing NM_001108133 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTCCTTGGATCACATGCTGACAGATCCCCTAGAACTTGGTCCTTGTGGAGATGGTCACAGTACAG GGATCATGGAGGACTGCCTTCTGGGTGGAACCAGGGTTAGTCTGCCTGAAGACCTGCTGGAGGATCCTGA AATATTCTTTGACGTCGTCAGCCTCTCCACATGGCAGGAAGTACTAAGTGACTCTCAGCGTGAACATCTC CAGCAGTTTCTGCCGCAGTTTCCTACAGACACTGTAGAGCAGCAGAATGAGCTCATCCTTGCCCTATTCA GTGGGGAGAACTTCCGCTTCGGAAACCCTCTGCACATCGCCCAGAAGCTCTTCCGAGATGGACACTTTAA CCCTGAGGTGGTCAAGTATCGGCAGCTCTGCTTCAAGTCACAGTACAAGCGGTACCTCAGCTCCCAACAG CAGTATTTCCACCGGCTGCTGAGACAGATTCTTGCTTCCAGAAGTGATCTCCTGGAGATGGCTCGCACGA GCGGCCCGGCTCTTCCCTTTCGACACAAGCACCATTCACCATCCCGAAGCCCGGAGGAGCGGGAGTGGCG GACCCAGCAGCGTTACCTGAAAGTCTTGAGGGAAGTGAAGGAGGAGTGTGGCGACACTGCCCTGTCGTCT GATGAGGAGGATCTCAGCTCATGGCTTCCAAGCTCTCCAGCACGCTCTCCTAGTCCTGCGGTGCCCCTGA GGGTGGTGCCCACGCTTTCCACCACGGATATGAAAACTGCAGATAAAATAGAACTGGGGGACAGTGACCT GAAGTTAATGTTAAAGAAACACCATGAGAAGCGGAAACATCAACCAGATCACCCAGACCTTTTGACAGGG GATCTGACTCTCAGCGACATCATGACTCGAGTAAATGCCGGCAGGAAGGGCTCTCTAGCAGCCTTGTATG ATTTGGCTGTTCTTAAAAAAAAGGTGAAGGAAAAGGAGGAGAAGAAAAAGAAGAAAATAAAGTTGATTAA ATCGGAGGCAGAGGATCTGGCTGAGCCCCTAAGCAATACTGAAGGGGTCCCAACGCTCTCCCAGGCCCCC TCTCCCCTGGCAATATCGTCTATCAAGGAAGAACCCCTGGAAGACATCAAGCCTTGTCTTGGAATCAATG AAATATCTTCCAGTTTTTTCTCTCTTCTGTTAGAGATCTTGCTGCTGGAGAGTCAGGCTAGCCTTCCTAT GCTGGAGGACCGGGTTTTGGATTGGCAGTCTTCTCCAGCCAGCTCCCTCAACAGCTGGTTCTCTGCTGCC CCCAACTGGGCTGAGTTGGTGTTGCCTGCTCTGCAGTATCTTGCTGGAGAAAGCCGAGCAGTTCCTTCCA GTTTTTCTCCATTTGTTGAATTCAAAGAGAAAACCCAGCAGTGGAAATTGCTTGGTCAATCTCAAGATAA TGAAAAGGAATTAGCCGCTCTCTTCCACTTGTGGCTAGAAACCAAAGACCAGGCTTTCTGTAAGGAAAAT GAAGACAGCTCAGATGCCATGACACCTGTGCCTCGAGTAAGAACTGACTATGTGGTTCGGCCTAGCACAG GAGAGGAGAAACGGGTTTTTCAAGAGCAGGAGCGTTACAGGTATAGCCAACCCCATAAGGCATTCACCTT TCGCATGCATGGCTTTGAGTCTGTGGTGGGGCCAGTGAAGGGCGTGTTTGACAAGGAGACCTCCCTCAAC AAGGCTCGTGAGCACTCACTGCTGCGCTCTGACCGGCCTGCCTATGTCACCATTCTGTCTCTTGTTCGGG ATGCTGCGGCTCGGCTGCCTAATGGAGAAGGCACTCGGGCAGAGATCTGTGAGCTGCTCAAGGACTCTCA GTTTCTTGCTCCTGATGTCACCAGCACTCAGGTGAACACGGTAGTGAGTGGCGCACTGGATCGATTGCAT TATGAGAAGGACCCTTGTGTGAAATACGACATTGGTCGGAAGCTGTGGATCTACCTGCATCGTGACCGGA GTGAGGAAGAGTTTGAACGGATCCATCAAGCTCAAGCAGCAGCAGCTAAAGCCAGAAAAGCTCTTCAGCA AAAACCCAAACCGCCATCCAAGGTGAAGTCCAGTAATAAGGAGGGCTCCGTGAAGGGCCTTAGTGGTCCT TCTGAGCCCAGCCAGATGAGCCTCAGTGACTCCAGCATGCCACCTACCCCAGTTACGCCTGTAACCCCCA CCACGCCAGCATTGCCTACCCCTATCTCTCCTCCACCTGTGTCAGCGGTGAGCAGAAGTGGCTCTACCAC TGTCTCTGAGCCAGCCAAGTCTAGTTCAGGTGTTCTTCTGGTGTCCTCACCAACAATGCCACAGCTAGGA ACGATGCTTTCCCCTGCTTCCATCCAGACTCCGCCCAGTTCTCAGACCACTGCTCGGGTTGTCAGCCATT CCAGCTCAGCAGGACTGCCCCAGGTTCGGGTAGTAGCCCAGCCCAGCCTTCCTGCTGTTTCCCAGCAGTC AGTAGGGCCAGCACAGCCACTACCCCAGATGCCAGCAGGACCACAGATCCGTGTGCCAGTCACCGCTACC CAAACCAAAGTAGTACCCCAGGCAGTTGTGGCTACAGTTCCAGTCAAGGGGCAGACTACAGCGGCCTCTG TGCAACGGCCTGGACCTGCACAGACAGGACTTACAGTGACGAGTCTTCCTGCTGCAGTTAGCCCCGGGAG CAAGACAGCCATGAGTTCTCCTGGGAGCTCTGCTCCAAGTGCCTCCACAACCGCCGTCATTCAGAATGTC ACAGGACAGAACATCATCAAGCAGGTGTCAATCACTGGACAGCTTGGTGTGAAGCCCCAGACAGGCAACA GCATTCCACTCACAGCCACAAACTTCCGTATCCAGGGTAAAGATGTACTGCGCCTGCCGCCTTCTTCCAT CACCACAGATGCCAAGGGCCAGACGGTTCTGAGAATCACTCCAGACATGATGGCCACACTGGCCAAGTCT CAGGTCACCACAGTCAAGTTGACTCAGGACCTCTTTGGGGCAGGTAGCGGCACTGCAGGCAAAGGCATCT CTGCTACCTTGCATGTGACTTCCAACCCTGTCCATGCGGCTGACAGCCCTGCCAAGGCCCCTTCAGCCAG TGTTCCTTCATCAGCTCCAGCGGGCACTACCGTGGTCAAAGTAACTCCTGATCTCAAGCCAACAGAAACC TCCAGTTCAGCTTTTCGCTTGATGCCAGCTCTTGGCGTGAGTGTGGCAGATCAGAAGGGGAAGAACACAG TGGCCTCTTCAGAAGCAAAACCTGCTGCCACAATCCGCATTGTGCAGGGGCTGGGAGTGATGCCTCCTAA AGCAGGCCAGACCATTACTGTTGCAGCACATGCAAAGCAAGGAGCCTCTGTTGCTGGTGGGTCTGGAACT GTCCATTCTTCAACTGTGTCCTTACCCAGTATCAATGCCACTGTGTCTAAGACTGTGGCTGTGGCTTCTG GAGCAACAAGCACCCCCATCAGCATCGGGACTGGAGCCCCCACCGTGCGACAGGTTCCTGTTAACACTAC AGTTGTGTCCACATCCCAGTCTGGGAAGCTGCCTACCAGGATCACAGTTCCTCTCTCTGTGATTAGCCAG CCAATGAAGGGCAAGAGCGTGGTCACAGCCCCCATCATCAAAGGCAACCTTGGAGCCAATCTCAGTGGGC TGGGTCGCAACATCATCCTCACAACCATGCCAGCGGGTGCTAAGCTCATTGCTGGCAATAAGCCAGTGAG TTTCCTCACCGCCCAGCAGTTGCAGCAGCTTCAGCAGCAAGGTCAGGCTACACAGGTACGCATCCAGACT GTGCCAGCATCCCATCTGCAACAGGGCACAGCTTCCGGCTCCTCCAAAGCCGTGTCCACTGTTGTTGTGA CCACAGCTCCATCTCCTAAACAAGCACCTGAGCAGCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR213386 representing NM_001108133 Red=Cloning site Green=Tags(s) MDSLDHMLTDPLELGPCGDGHSTGIMEDCLLGGTRVSLPEDLLEDPEIFFDVVSLSTWQEVLSDSQREHL QQFLPQFPTDTVEQQNELILALFSGENFRFGNPLHIAQKLFRDGHFNPEVVKYRQLCFKSQYKRYLSSQQ QYFHRLLRQILASRSDLLEMARTSGPALPFRHKHHSPSRSPEEREWRTQQRYLKVLREVKEECGDTALSS DEEDLSSWLPSSPARSPSPAVPLRVVPTLSTTDMKTADKIELGDSDLKLMLKKHHEKRKHQPDHPDLLTG DLTLSDIMTRVNAGRKGSLAALYDLAVLKKKVKEKEEKKKKKIKLIKSEAEDLAEPLSNTEGVPTLSQAP SPLAISSIKEEPLEDIKPCLGINEISSSFFSLLLEILLLESQASLPMLEDRVLDWQSSPASSLNSWFSAA PNWAELVLPALQYLAGESRAVPSSFSPFVEFKEKTQQWKLLGQSQDNEKELAALFHLWLETKDQAFCKEN EDSSDAMTPVPRVRTDYVVRPSTGEEKRVFQEQERYRYSQPHKAFTFRMHGFESVVGPVKGVFDKETSLN KAREHSLLRSDRPAYVTILSLVRDAAARLPNGEGTRAEICELLKDSQFLAPDVTSTQVNTVVSGALDRLH YEKDPCVKYDIGRKLWIYLHRDRSEEEFERIHQAQAAAAKARKALQQKPKPPSKVKSSNKEGSVKGLSGP SEPSQMSLSDSSMPPTPVTPVTPTTPALPTPISPPPVSAVSRSGSTTVSEPAKSSSGVLLVSSPTMPQLG TMLSPASIQTPPSSQTTARVVSHSSSAGLPQVRVVAQPSLPAVSQQSVGPAQPLPQMPAGPQIRVPVTAT QTKVVPQAVVATVPVKGQTTAASVQRPGPAQTGLTVTSLPAAVSPGSKTAMSSPGSSAPSASTTAVIQNV TGQNIIKQVSITGQLGVKPQTGNSIPLTATNFRIQGKDVLRLPPSSITTDAKGQTVLRITPDMMATLAKS QVTTVKLTQDLFGAGSGTAGKGISATLHVTSNPVHAADSPAKAPSASVPSSAPAGTTVVKVTPDLKPTET SSSAFRLMPALGVSVADQKGKNTVASSEAKPAATIRIVQGLGVMPPKAGQTITVAAHAKQGASVAGGSGT VHSSTVSLPSINATVSKTVAVASGATSTPISIGTGAPTVRQVPVNTTVVSTSQSGKLPTRITVPLSVISQ PMKGKSVVTAPIIKGNLGANLSGLGRNIILTTMPAGAKLIAGNKPVSFLTAQQLQQLQQQGQATQVRIQT VPASHLQQGTASGSSKAVSTVVVTTAPSPKQAPEQQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001108133
ORF Size	3888 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001108133.1, NP_001101603.1
RefSeq Size	5179 bp
RefSeq ORF	3891 bp
Locus ID	315523
MW	138.7 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.