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Gdf1 (NM_001044240) Rat Tagged ORF Clone
CAT＃: RR213368
- TrueORF®
Gdf1 (Myc-DDK-tagged ORF) - Rat growth differentiation factor 1 (Gdf1), (10 ug)
"NM_001044240" in other vectors (3)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR213368 representing NM_001044240
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCCCTGTCTGCCACCGTTTTTGCGACCACCTCCTCCTTCTGCTCTTGCTGCCCTCAACGACCCTGG CCCACGCGCCAGCATCCTTGGGCCCCGCTGCCGCCCTGCTCCAGGTTCTCGGGCTTCCCGAAACGCCCCG GAGCGTCCCCACACTCCGACCTGTGCCTCCTGTCATGTGGCGCCTATTCCGCAGCCGCAACCCCCAGGAG GCCAGAGTGGGGCGCCCTCTGAAACCATGCCACGTGGAGGAACTGGGGGTTGCCGGAAACATTGTGCGCC ACATCCCCGACAGCGGTCTGTCCTCCAGGATCATACAGCCCGCCAGAACCTCAGGGCTGTGCCCCGAGTG GACCGTCGTCTTTGACCTGTCGAGCGTGGAGCCCATAGAGCGCCCAACACGCGCGCGCCTAGAGTTGCGG CTGGAGGCTGAGAGCGAAGATACACGAGGGTGGGAGCTAAGCGTGGCGCTGTTGGCCGAGGCTGAGCATC CTGGGCCTGAGCTACTGCGCGTGCAGGCGCCACCCGGGCTGCCACTGCGCGCAGACCTACTGGGGACTGC AGTAGCGGCTAACGCATCGGTGCCGCGCACTCTGCGCTTGGCGCTGGCGCTGCACCCTGGGGCCGCTGCA ACCTGTGGGCGCCTGGCTGAGGCCTCGTTGCTGCTGGTGACGCTGGACCCACGCCTGTGTCCCTTGCCGC GGTCACGGCGCCACACGGAGCCCAGGGTGGGAGGTGGTCCAGTGGGCACATGTCGCACCCGACGGTTGCA CGTGAGCTTCCGTGAGGTGGGCTGGCACCGCTGGGTGATTGCGCCGCGGGGCTTTCTAGCCAACTTCTGC CAGGGCACGTGTGCGCTACCTGAAACACTGAGGGGACCCGGCGGGCCGCCTGCACTTAACCACGCTGTGC TGCGCGCGCTCATGCACGCAGCTGCTCCCACCCCGGGTGTAGGCTCGCCCTGCTGCGTGCCAGAGCGTCT GTCGCCTATCTCCGTGCTCTTCTTCGATAATAGTGACAACGTGGTGCTGCGCCACTACGAGGACATGGTG GTGGATGAGTGTGGCTGTCGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR213368 representing NM_001044240
Red=Cloning site Green=Tags(s) MLPVCHRFCDHLLLLLLLPSTTLAHAPASLGPAAALLQVLGLPETPRSVPTLRPVPPVMWRLFRSRNPQE ARVGRPLKPCHVEELGVAGNIVRHIPDSGLSSRIIQPARTSGLCPEWTVVFDLSSVEPIERPTRARLELR LEAESEDTRGWELSVALLAEAEHPGPELLRVQAPPGLPLRADLLGTAVAANASVPRTLRLALALHPGAAA TCGRLAEASLLLVTLDPRLCPLPRSRRHTEPRVGGGPVGTCRTRRLHVSFREVGWHRWVIAPRGFLANFC QGTCALPETLRGPGGPPALNHAVLRALMHAAAPTPGVGSPCCVPERLSPISVLFFDNSDNVVLRHYEDMV VDECGCR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001044240 |
| ORF Size | 1071 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001044240.2, NP_001037705.1 |
| RefSeq Size | 2683 bp |
| RefSeq ORF | 1074 bp |
| Locus ID | 306351 |
| MW | 38.6 kDa |
| Gene Summary | This gene encodes a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site that is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. The mouse ortholog of this gene is involved in the establishment of left-right asymmetry in early embryogenesis and in neural development in later embryogenesis. This protein is transcribed from a bicistronic mRNA that also encodes the LAG1 homolog, ceramide synthase 1 gene. Mono-cistronic transcripts for this gene are also found in mouse, but it is not known if they exist in rat. [provided by RefSeq, Jul 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN213368 | Gdf1 (untagged ORF) - Rat growth differentiation factor 1 (Gdf1), (10 ug) |
CNY 3,990.00 |
|
| RR213368L3 | Lenti ORF clone of Gdf1 (Myc-DDK-tagged ORF) - Rat growth differentiation factor 1 (Gdf1), (10 ug) |
CNY 6,080.00 |
|
| RR213368L4 | Lenti ORF clone of Gdf1 (mGFP-tagged ORF) - Rat growth differentiation factor 1 (Gdf1), (10 ug) |
CNY 6,650.00 |
