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Tsc1 (NM_021854) Rat Tagged ORF Clone
CAT＃: RR213270
- TrueORF®
Tsc1 (Myc-DDK-tagged ORF) - Rat tuberous sclerosis 1 (Tsc1), (10 ug)
"NM_021854" in other vectors (3)
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CNY 18,720.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR213270 representing NM_021854
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCAGCTAGCCAACATTGGGGAGCTACTCTCCATGCTGGACTCTTCCACACTGGGTGTGCGTGATG ACGTGACAACCATCTTCAAGGAGTCCCTCAATTCTGAACGTGGGCCTATGCTTGTAAACACGTTGGTGGA TTATTACCTGGAAACCAATTCTCAGCCGGTCTTGCACATCCTGACCACCCTGCAAGAGCCACATGATAAG CACCTCTTGGACAAAATGAATGAGTATGTAGGCAAAGCTGCCACCCGCTTATCCATCCTCTCGCTGCTGG GGCATGTTGTGAGGCTGCAGCCATCTTGGAAGCATAAGCTCTCTCAAGCACCTCTTCTGCCTTCTTTACT GAAATGTCTCAAGATGGACACTGATGTTGTGGTTCTCACAACTGGTGTCTTGGTGTTGATCACCATGCTA CCGATGATCCCGCAGTCAGGGAAGCAGCACCTTCTCGACTTCTTTGACATCTTTGGCCGTCTTTCATCAT GGTGCCTGAAGAAACCAGGCCATGTGACAGAAGTCTACCTGGTCCACCTCCATGCCAGTGTTTATGCCCT CTTCCATCGCCTTTATGGAATGTACCCTTGTAACTTCGTCTCCTTCCTGCGCTCTCACTACAGCATGAAG GAAAACGTGGAGACTTTTGAAGAAGTGGTCAAGCCAATGATGGAGCATGTGCGAATTCACCCGGAATTAG TGACTGGATCCAAGGACCATGAACTGGACCCTCGAAGGTGGAAGACATTAGAAACTCATGATGTTGTAAT AGAGTGTGCCAAAATCTCTCTGGACCCTACCGAAGCCTCATATGAAGATGGCGATGCTGTGTCTCACCAG CTCTCTGCCTGCTTCCCTCACCGTTCAGCTGATGTCACCACCAGCTCTTACGTGGACACACAGAATAGCT ATGGGGGCGCTACTTCCACCCCTTCCTCCACCTCTCGGCTGATGTTGTTCAGTACCCCCGGGCAGCTACC TCAGAGTTTGAGTTCACTATCAACACGGCCGTTACCTGAGCCGCTGCAAGCTAGTCTTTGGAGCCCATCT GCAGTCTGTGGTATGACCACTCCTCCTACGTCTCCTGGAAATGTCCCGGCTGATTTGTCACATCCATACA GTAAAGCCTTTGGTACTACCACTGGTGGAAAAGGAACTCCTTCAGGAACCCCAGCGACCTCTCCTCCCCC AGCCCCGCCTTGTCCCCAAGACGACTGTGCGCACGGTCCAGCCTCCCAGGCCTCAGCCACACCCCCCAGG AAGGAAGAAAGAGCAGATTCTTCAAGGCCTTACCTACCCAGACAGCAGGATGTTCCGAGTGACCGCGGAT TAGAGGATCTACCTGGAAGCAAAGGTTCCGTCACTCTGAGGAATCTACCAGATTTCCTAGGTGACCTGGC TTCAGAGGAAGACAGTATAGAGAAAGATAAGGAAGAAGCTGCGATATCTAAAGAGCTTTCTGAGATCACC ACTGCAGAGGCGGATCCTGTAGCTCCTCGAGGGGGCTTTGACTCTCCCTTCTACCGAGACAGTCTCTCTG GTTCTCAGCGGAAGACTCACTCAGCAGCCTCTGGGACTCAGGGCTTCAGCGTGAACCCTGAGCCTTTGCA CTCCTCCCTGGACAAACATGGGCCTGACACACCAAAGCAAGCCTTTACTCCCATAGACCCACCCTCTGGC AGTGCTGATGCCAGTCCCGCTGGGGACAGGGATCGCCAGACTTCTCTGGAGACCAGTATCCTCACTCCCA GCCCTTGCAAAATTCCACCTCAGAGGGGAGTGAGCTTTGGAAGTGGGCAGCTTCCCCCATATGATCATCT CTTTGAGGTGGCCTTGCCAAAGACTGCCTGTCACTTTGTCAGCAAAAAGACTGAAGAGCTGTTGAAGAAA GCGAAAGGAAACCCTGAGGAAGACTGTGTGCCCTCTACCTCCCCAATGGAAGTACTGGACAGACTGCTAG AGCAGGGAGCAGGGGCACACAGCAAGGAACTGAGCAGGTTGTCCTTGCCCAGCAAGTCTGTTGACTGGAC CCACTTTGGAGGCTCTCCCCCCTCAGATGAGATCCGGACCCTCCGAGACCAGTTGCTCTTACTGCACAAT CAGCTGCTGTACGAACGCTTTAAGCGGCAGCAGCATGCACTGAGGAACAGAAGGCTGCTGCGCAAGGTGA TCCGAGCTGCGGCCCTGGAGGAGCACAACGCAGCGATGAAAGATCAGTTGAAGTTACAAGAGAAGGACAT CCAGATGTGGAAGGTGAGTCTGCAGAAAGAACAAGCCCGATACAGTCAGCTTCAGCAACAACGTGACACC ATGGTGACCCAGCTGCATAGCCAAATCAGACAGCTGCAGCATGACCGAGAAGAATTCTACAACCAGAGTC AGGAGTTACAGACGAAGCTGGAGGACTGCAGAAGCATGATCGCGGAGCTTCGAGTGGAGCTGAAGAAGGC CAACAGCAAGGTGTGCCACACCGAGCTGCTGCTCAGCCAGGTCTCTCAGAAGCTCTCCAATAGTGAGTCA GTGCAGCAGCAGATGGAGTTCTTGAATAGGCAGCTCCTGGTGCTCGGGGAGGTCAATGAGCTGTACCTGG AGCAGCTGCAGAGCAAGCATCCTGACACCACCAAGGAAGTAGAAATGATGAAAACTGCTTATCGGAAAGA ACTAGAGAAAAACAGAAGCCACCTTCTCCAGCAGAACCAGAGGCTGGATGCCTCACAGAGGCGAGTTTTA GAACTGGAATCTCTTCTGGCCAAGAAAGACCACCTTCTCCTAGAGCAGAAGAAGTACCTTGAGGATGTCA AGAGCCAGGCGAGTGGACAGCTGCTGGCTGCAGAGAGCAGGTATGAGGCTCAGAGAAAGATCACCCGGGT GTTGGAACTGGAGATCCTAGACTTGTATGGTAGATTGGAGAAAGATGGCCGCCTACAGAAACTAGAAGAG GACAGAGCAGAGGCAGCAGAGGCAGCTGAAGAGAGGCTTGACTGTTGTACTGATGGATGCTCAGATTCCT TGTTAGGACATAATGAAGAGGCTGCTGGTCACAATGGTGAGACCAGGACCTCCAGACCTGGTGGCACCCG GGCCAGCTGTGGAGGTAGAGTCACTGGAGGCAGCAGCAGCAGCAGCAGTGAGCTTTCCACTCCAGAGAAA CCCCCGAACCAGAGGTTCAGCAGCCGGTGGGAGCCCACCATGGGTGAGCCCTCCAGCAGCATCCCCACCA CTGTTGGCTCACTTCCCAGTTCCAAAAGCTTCTTGGGCATGAAGACCCGGGAGCTGTTCCGTAATAAGAG TGAGAGCCAGTGTGATGAGGATGGCATGACCATGAGTAGCTTTTCTGAGACCCTGAAGACAGAACTGGGC AAAGACTCGGCGGGCATGGAAAACAAGACTCCCCCAAGTCTAGACGCCCCACACCCATCTTCCCCAAGCT CAGACAGTATGGGGCAGCTCCATATCATGGACTACAATGAGACTCATCACGAACACAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR213270 representing NM_021854
Red=Cloning site Green=Tags(s) MAQLANIGELLSMLDSSTLGVRDDVTTIFKESLNSERGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDK HLLDKMNEYVGKAATRLSILSLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVVVLTTGVLVLITML PMIPQSGKQHLLDFFDIFGRLSSWCLKKPGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMK ENVETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKTLETHDVVIECAKISLDPTEASYEDGDAVSHQ LSACFPHRSADVTTSSYVDTQNSYGGATSTPSSTSRLMLFSTPGQLPQSLSSLSTRPLPEPLQASLWSPS AVCGMTTPPTSPGNVPADLSHPYSKAFGTTTGGKGTPSGTPATSPPPAPPCPQDDCAHGPASQASATPPR KEERADSSRPYLPRQQDVPSDRGLEDLPGSKGSVTLRNLPDFLGDLASEEDSIEKDKEEAAISKELSEIT TAEADPVAPRGGFDSPFYRDSLSGSQRKTHSAASGTQGFSVNPEPLHSSLDKHGPDTPKQAFTPIDPPSG SADASPAGDRDRQTSLETSILTPSPCKIPPQRGVSFGSGQLPPYDHLFEVALPKTACHFVSKKTEELLKK AKGNPEEDCVPSTSPMEVLDRLLEQGAGAHSKELSRLSLPSKSVDWTHFGGSPPSDEIRTLRDQLLLLHN QLLYERFKRQQHALRNRRLLRKVIRAAALEEHNAAMKDQLKLQEKDIQMWKVSLQKEQARYSQLQQQRDT MVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRSMIAELRVELKKANSKVCHTELLLSQVSQKLSNSES VQQQMEFLNRQLLVLGEVNELYLEQLQSKHPDTTKEVEMMKTAYRKELEKNRSHLLQQNQRLDASQRRVL ELESLLAKKDHLLLEQKKYLEDVKSQASGQLLAAESRYEAQRKITRVLELEILDLYGRLEKDGRLQKLEE DRAEAAEAAEERLDCCTDGCSDSLLGHNEEAAGHNGETRTSRPGGTRASCGGRVTGGSSSSSSELSTPEK PPNQRFSSRWEPTMGEPSSSIPTTVGSLPSSKSFLGMKTRELFRNKSESQCDEDGMTMSSFSETLKTELG KDSAGMENKTPPSLDAPHPSSPSSDSMGQLHIMDYNETHHEHS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021854 |
| ORF Size | 3489 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021854.1, NP_068626.1 |
| RefSeq Size | 3492 bp |
| RefSeq ORF | 3492 bp |
| Locus ID | 60445 |
| UniProt ID | Q9Z136 |
| MW | 129 kDa |
| Gene Summary | In complex with TSC2, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling (By similarity). Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling (PubMed:16707451). Acts as a co-chaperone for HSP90AA1 facilitating HSP90AA1 chaperoning of protein clients such as kinases, TSC2 and glucocorticoid receptor NR3C1 (By similarity). Increases ATP binding to HSP90AA1 and inhibits HSP90AA1 ATPase activity (By similarity). Competes with the activating co-chaperone AHSA1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (By similarity). Recruits TSC2 to HSP90AA1 and stabilizes TSC2 by preventing the interaction between TSC2 and ubiquitin ligase HERC1 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN213270 | Tsc1 (untagged ORF) - Rat tuberous sclerosis 1 (Tsc1), (10 ug) |
CNY 18,720.00 |
|
| RR213270L3 | Lenti ORF clone of Tsc1 (Myc-DDK-tagged ORF) - Rat tuberous sclerosis 1 (Tsc1), (10 ug) |
CNY 20,240.00 |
|
| RR213270L4 | Lenti ORF clone of Tsc1 (mGFP-tagged ORF) - Rat tuberous sclerosis 1 (Tsc1), (10 ug) |
CNY 22,230.00 |
